OBJECTIVE:To study the effect of Xiaoyao pill on animal uterus and to evaluate its efficacy in regulating menstruation to serve as theoretical basis for its clinical application.METHODS:The convulsion model of both ex vivo and in vivo uterus of rats was induced by oxytocin and the effects of Xiaoyao pill on the tension as well as the contraction frequency change of smooth muscle of uterus were observed.RESULTS:Xiaoyao pill could markedly inhibit the enhanced activities of either ex vivo or in vivo uterus of rats induced by oxytocin,inhibit the tension of smooth muscle and slow down the contraction frequency.CONCLUSION:Xiaoyao pill has satisfactory efficacy in regulating menstruation.

OBJECTIVE:To establish an HPLC method for determination of protocatechuic acid in Pterospermum heterophyllum.METHODS: The separation was performed on Agilent TC C18(250 mm?4.6 mm,5.0 ?m)column.Moblie phase consisted of acetonitrile-0.1% glacial acetic acid solution(pH 3.16,10 ∶ 90)with flow rate of 1.0 mL?min-1 and detection wavelength of 260 nm.The column temperature was set at 35℃ and injection volume was 10 ?L.RESULTS:The linear range of protocatechuic acid was 0.007 76～0.310 4 ?g(r=0.999 7) with an average recovery of 97.68% (RSD=1.51%,n=6).CONCLUSION:The method is simple,accurate and reproducible for the quality control of P.heterophyllum.

ObjectiveTo investigate the epidemiological trends of influenza in Yiwu City， Zhejiang Province from 2017 to 2022， and to provide references for local adjustments to influenza prevention and control strategies. MethodsReal-time fluorescence quantitative PCR was used to detect and type influenza viruses in nasal and pharyngeal swab specimens of the patients with influenza-like illness （ILI）. The results were statistically analyzed. ResultsFrom 2017 to 2022， a total of 106 661 ILI cases were reported in Yiwu City， with annual reported cases of 31 273， 33 522， 20 090， 9 965， 3 202， and 8 609， respectively. The majority of ILI cases were in the age group of ≤14 years， accounting for 89.16%. A total of 6 893 specimens were collected and tested， of which 945 were tested positive for influenza virus nucleic acid， with an overall positivity rate of 13.71%. The dominant subtypes were H3N2 and B⁃Victoria， accounting for 40.63% （384/945） and 35.03% （331/945） respectively. The highest positivity rate was in 2019， at 25.19% （265/1 052）. The positivity rates significantly decreased in 2020 and 2021， to 5.74% （66/1 149） and 5.77% （75/1 300）， respectively. The rate increased in 2022 to 13.85% （180/1 300）. The highest proportion of A/H3N2 positivity was in 2017， at 69.14% （168/243）. The highest proportion of A/H1N1 positivity was in 2018，at 50.86%（59/116）. The highest proportion of B/Victoria positivity was in 2021， at 100.00% （75/75）. The highest proportion of B/Yamagata positivity was in 2018， at 5.17% （6/116）. ConclusionInfluenza in Yiwu City exhibits obvious seasonal patterns， with two peaks in winter-spring and summer. Dominant strains such as A/H3N2， B/Victoria， and A/H1N1 alternate or co-circulate. During the COVID-19 pandemic， seasonal influenza significantly decreased， and a series of prevention and control measures had a positive effect on influenza prevention and control.