1.In vitro glycosylation of Vitamin C
Byambasuren Dorjsuren ; Bolor Buyanbadrakh ; Jae Kyung Sohng
Mongolian Medical Sciences 2014;167(1):7-9
BACKGROUND: Glycosylation process helps in stabilization and solubilization natural of compounds.
Glycosyltransferase (YjiC) provides for high efficient glycosylation product with an incredible variety
of sugar moieties, typically from UDP-glucose. Vitamin C (L-ascorbic acid) is an essential nutrient
for humans and certain other animal species. Vitamin C functions in many biological processes,
such as collagen synthesis, antoxidation, intestinal absorption of iron. UDP-glucose acts as a
starting material for glycosyltransferase (YjiC). In order to recycle UDP-glucose after glycosylation
with glycosyltransferase (YjiC), sucrose synthase (AtSUS1) carry out than reversible conversion of
sucrose and UDP to UDP-glucose and fructose.
MATERIALS AND METHODS: DNA was extracted than E.coli BL 21 and E.coli JM 109 hosts were used
for expression of proteins. The purified protein was then analyzed by 12% SDS-PAGE than used for
enzymatic recycle system. TLC analyse of the products were carried out to the test glycosylation.
RESULTS: In this study, we choose substrate vitamin C for the enhancement of enzymatic recycling
system glycosylation. In this recycle system due to the high concentration of sucrose and vitamin C
but low concentration of UDP-glucose with is relatively expensive made the system more economic.
TLC analyses of the products were carried out to the recycled system worked and glycosylation
product.
CONCLUSION: Based on proved function an enzymatic recycling system with glycosyltransferse (YjiC)
and sucrose synthesis (AtSUS1) to be applicable to enzymatic production of vitamin C glucoside
and resveratrol glucoside. Further analysis by HPLC and MS will elucidate the products.
Result Analysis
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