Objective To explore the correlation between hepatitis B virus large surface protein(HBV-LP)and hepatitis B virus deoxyribonucleic acid(HBV-DNA),analyze the clinical significance of detecting the two index dynamically for diagnosis and treatment of hepatitis B.Methods Enzyme linked immunosorbent assay(ELISA)and fluorescent quantitation polymerase chain reaction(FQ-PCR)were used to detect the levels of HBV-LP and HBV-DNA in serum specimen of 230 hepatitis B patients.Results There was a positive correlation between the content of HBV-LP and copy numbers of HBV-DNA in serum of hepatitis B positive cases(r=0.84,P＜0.01).The positive rate was 84.78％ in HBV-LP and 84.35％ in HBV-DNA of 230 HBV positive cases.As a result there was no significant difference(P＞0.05)in this study.Therefore,the positive rate was 63.63 ％(35/55)in HBV-LP and 58.18％(32/55)in HBV-DNA of 55 HBV negative cases.There was also no significant difference(P＞0.05)in this study.However,the positive rate of HBV-LP(82.47％)was higher than HBE(52.06％)in the 194 HBV-DNA positive cases.There was significantly different(P＜0.01).Conclusion HBV-LP and HBV-DNA are the significant index of the degree of hepatitis B virus replication in hepatitis B positive cases,especially in determining the virus replication and prognosis of treatment in HBe Ag negative,providing the reliable laboratory data for the clinical diagnosis and treatment.

Objective:To investigate the diagnosis and treatment of occupational acute methanol poisoning.Methods:Retrospective analysis of the clinical data of 5 cases of occupational acute me thanol poisoning admitted from October 11 to 12, 2018.Results:The first patient was diagnosed with severe acute methanol poisoning and died after treatment with mechanical ventilation, hemodialysis, and detoxification by ethanol and folic acid for 38 hours. The remaining four cases were all diagnosed with mild acute methanol poisoning and were discharged from hospital after active symptomatic support treatment for 63 to 69 hours. Fuhermore, all the four patients were followed up for one year and without sequelaes.Conclusion:Early evaluation of the disease, early combination with hemodialysis, and use of detoxification drugs are the key to rescue occupational acute methanol poisoning.

Objective:To investigate the related factors affecting the recovery of cholinesterase (ChE) activity in patients with acute chlorpyrifos poisoning.Methods:In February 2020, the clinical data of acute chlorpyrifos poisoning patients admitted in our hospital from January 2016 to December 2019 were retrospectively analyzed. The outcome variable was the time of ChE activity recovered to 50% lower limit of normal value, and multivariate linear regression analysis was performed to explore its influencing factors.Results:A total of 78 patients, 43 males and 35 females, with an average age (39.58±14.77) years were enrolled in this study. The average time of serum ChE activity recovered to 50% lower limit of normal value was (24.45±2.64) days. There was a correlation between hemoperfusion ( r=-0.644) , atropine dosage ( r=0.498) , chlorophosphorus dosage ( r=0.432) and the time of serum ChE activity recovered to 50% lower limit of normal value, in which hemoperfusion was significantly negatively correlated with the time of serum ChE activity recovered to 50% lower limit of normal value ( β=-4.222, P<0.05) . Conclusion:The recovery of serum ChE activity in patients with acute chlorpyrifos poisoning is very slow. Hemoperfusion can quickly remove chlorpyrifos, its metabolites and inflammatory mediators in the blood, thus effectively promoting the recovery of ChE activity.

Objective:To investigate the diagnosis and treatment of occupational acute methanol poisoning.Methods:Retrospective analysis of the clinical data of 5 cases of occupational acute me thanol poisoning admitted from October 11 to 12, 2018.Results:The first patient was diagnosed with severe acute methanol poisoning and died after treatment with mechanical ventilation, hemodialysis, and detoxification by ethanol and folic acid for 38 hours. The remaining four cases were all diagnosed with mild acute methanol poisoning and were discharged from hospital after active symptomatic support treatment for 63 to 69 hours. Fuhermore, all the four patients were followed up for one year and without sequelaes.Conclusion:Early evaluation of the disease, early combination with hemodialysis, and use of detoxification drugs are the key to rescue occupational acute methanol poisoning.

Objective:To investigate the related factors affecting the recovery of cholinesterase (ChE) activity in patients with acute chlorpyrifos poisoning.Methods:In February 2020, the clinical data of acute chlorpyrifos poisoning patients admitted in our hospital from January 2016 to December 2019 were retrospectively analyzed. The outcome variable was the time of ChE activity recovered to 50% lower limit of normal value, and multivariate linear regression analysis was performed to explore its influencing factors.Results:A total of 78 patients, 43 males and 35 females, with an average age (39.58±14.77) years were enrolled in this study. The average time of serum ChE activity recovered to 50% lower limit of normal value was (24.45±2.64) days. There was a correlation between hemoperfusion ( r=-0.644) , atropine dosage ( r=0.498) , chlorophosphorus dosage ( r=0.432) and the time of serum ChE activity recovered to 50% lower limit of normal value, in which hemoperfusion was significantly negatively correlated with the time of serum ChE activity recovered to 50% lower limit of normal value ( β=-4.222, P<0.05) . Conclusion:The recovery of serum ChE activity in patients with acute chlorpyrifos poisoning is very slow. Hemoperfusion can quickly remove chlorpyrifos, its metabolites and inflammatory mediators in the blood, thus effectively promoting the recovery of ChE activity.