Objective : To explore whether miR⁃9 affects the proliferation of neural stem cells (NSCs) by regulating β ⁃tubulin Ⅲ and glial fibrillary acidic protein (GFAP) . Methods : NSCs cells were isolated and cultured on the purchased healthy pregnant mice and the isolated cells were divided into NO group ( non⁃transfected NSCs cell line) , NN group (NSCs transfected with miR⁃9 ⁃NC) , NM group (NSCs transfected) transfected with miR⁃9 mimics) , NI group ( transfected with miR⁃9 inhibitor) . Nestin was identified by immunofluorescence , the contents of Mir⁃9 , β ⁃tubulin Ⅲ and GFAP were measured by QRT⁃PCR , the proliferation of NSCs was measured by MTT method , the apoptosis of NSCs was measured by flow cytometry , and the protein expressions of Mir⁃9 , β ⁃tubulin Ⅲ and GFAP were detected by Western blot. Results : The detection index Nestin of NSCs for 24 h after cell passage was positive in 90% of the NSCs , and co⁃localized with the nucleus , indicating the successful isolation of NSCs. The expression of miR⁃9 mRNA in NSCs of the NM group was the highest among the four groups , indicating that the transfection was successful. Among the four groups , β ⁃tubulin Ⅲ mRNA and protein expression was the highest in the NM group , and the GFAP mRNA and protein expression was the lowest. The NI group was the opposite (P < 0. 05) . In the four groups after 24 h , the cell activity of NSCs in the NM group was the strongest and showed a trend of gradually increasing with time (P < 0. 05) . The cell activity of NI was significantly weaker than that of the other three groups (P < 0. 05) . Among the four groups , the apoptosis rate of the NM group was lower than that of the other three groups (P < 0. 05) , and the apoptosis rate of NI was higher than that of the other three groups (P < 0. 05) . The difference in the above indicators between the NO group and the NN group was smaller. Conclusion Overexpressed miR⁃9 promotes the proliferation of NSCs by promoting the expression of β ⁃tubulin Ⅲ and inhibiting the expression of GFAP.

Objective To study the effect of fascia parotideomasseterica reconstruction in the prevention of Frey syndrome.Methods 45 patients who suffered parotidectomy with parotid fascia maintained and reconstructed were followed-up,the OccurrenCe rate of Frey syndrome wasobserved.Results The occurrence rate of the subjective symptom8 with Frey syndrome Was 6.7%(3/45)6 montes after surgery;the occurrence rate of Frey syndrome was 11.1%(5/45)by Minor test review 1 year after surgery;the remaining 41 cases were non-induced reactlons.Condusion Fascia parotidcomassetefica could reduce the development of Frey syndrome in the parotid gland surgery in pafients with benign tumors.