Objective: To approach a better solution for enhancing the therapeutic results of acupuncture therapy in the treatment of Tourette syndrome, by observing the clinical results of combined scalp with body acupuncture and mono-body acupuncture. Methods: Fifty-seven patients were randomized into a treatment group (31 cases) and a control group (26 cases). The patients in the treatment group all received combined scalp-body acupuncture treatment, while the patients in the control group were given mono-body acupuncture treatment, for 1 month as a treatment session. At the end of the third treatment session, the Yale Global Tic Severity Scale (YGTSS) would be compared between pre- and post-treatment. Results: In the treatment group, 2 patients were clinically cured, 4 showed markedly effective, 18 showed effective, and 7 failed, making a total therapeutic rate of 77.4%. In the control group, 0 were clinically cured, 3 showed markedly effective, 9 showed effective, 14 failed, making a total therapeutic rate of 46.2%. There was a significant difference between the two total therapeutic rates (P<0.05). Conclusion: The combination of scalp and body acupuncture had a better therapeutic result than the mono-body acupuncture therapy in the treatment Fifty-of Tourette syndrome.

The treatments for insomnia after stroke were various in recent years, including acupuncture with its distinguishing feature. It’s reviewed and summarized that experimental researches on Chinese and English databases about acupuncture treatment of insomnia after stroke in recent 10 years. Clinicians had chosen different acupuncture therapies for insomnia after stroke according to yinyang, zangfu, meridians, opening and closing of qi activity. Although those acupuncture therapies had achieved obvious clinical effects, they were deficient in experimental design and methodology.

Objective To investigate the effects and mechanisms of a disintegrin and metalloproteinase 17 (ADAM17) on high-glucose mediated permeability,proliferation and migration in human retinal microvascular endothelial cells (HRMECs).Methods HRMECs were divided into 4 groups:normal group (5 mmol · L-1 glucose),high glucose group (25 mmol · L-1 glucose),NC (Negative control for siRNA) + high glucose group and siADAM17 (ADAM17 siRNA) + high glucose group.The expression of ADAM17 was detected using real time PCR and Western blot.Horseradish Peroxidase (HRP) was used to detect the permeability of HRMECs.Cell Counting Kit-8 (CCK-8)and BrdU were used to evaluate cell proliferation.Cell migration was determined using Transwell assay.In addition,the expression of p-EGFR,p-ERK and MMP9 was assayed using Western blot.Results Compared with normal group,the mRNA and protein levels of ADAM17 were increased in high glucose group (P ＜ 0.01).ADAM17 expression of siADAM17 + high glucose group was markedly reduced compared with NC + high glucose group.High glucose increased the permeability of HRP comparison to normal group,whereas in siADAM17 + high glucose group the permeability of HRP was reduced compared with NC + high glucose group.The optical density of HRMECs was decreased in siADAM17 + high glucose group 1.53 ± 0.29 in comparison with NC + high glucose group 2.43 ± 0.25,as well as the content of BrdU-incorporation(P ＜ 0.05).The number of migrated cells in high glucose group,NC + high glucose group,siADAM17 + high glucose group and normal group were 157.00 ± 7.93,169.00 ± 10.12,121.00 ± 9.28,110.00 ±8.25,respectively.Moreover,the expression of p-EGFR,p-ERK and MMP9 in siADAM17 +high glucose group was decreased compared with NC + high glucose group (all P ＜0.01).Conclusion SiADAM17 can reduce the cell permeability,suppressed and migration induced by high glucose via EGFR/ERK/MMP9 signaling pathway.