With the method of fluid-filled stomach and intravenous administration of anisodaminal hydrochloridum(654-2), 94 cases of 11 gastroduodenal diseases including gastric cancer and others were examined with ultrasound. Comparison of the ultrasonography with the pathological changes was done. The finding rate of gastric foci which equal to or more than 5mm in diameter was 96.8%. The correct rate of diagnosis was 80.9%. The accuracy in differentiating early gastric cancer from advancing gastric cancer was 87.5%. The correct rate for judging invasive depth of stomach wall by gastric cancer was 82.9%. It can also show the distinct ultrasonic features for gastric submucosal diseases.

Humans ; Infant ; Milk, Human ; chemistry ; Paraquat ; poisoning ; Poisoning ; diagnosis

Objective: To detect the localization of HBV DNA in kidneys of HBV-associated glomerulonephritis, and investigate the pathogenesis of HBV-associated glomerulonephritis. Methods: 45 cases of renal biopsy specimens (38 cases had glomerular deposition of HBV antigens, 7 cases were negative for serologic and histologic HBV antigens) were examined for HBV DNA by in situ hybridization (ISH) and in situ PCR (IS-PCR). Results: The positive rate of HBV DNA in renal biopsy was 71% (27/38) in patients with glomerular deposition of HBV antigens. HBV DNA was found in 19 cases (19/26, 73%) of HBV-associated glomerulonephritis; and in 8 cases (8/12, 67%) of IgA nephropathy and lupus nephritis with glomerular deposition of HBV antigens. HBV DNA was detected mainly in the cytoplasm of tubular epithelia with ISH; IS-PCR showed that HBV DNA was localized not only in cytoplasm of tubular epithelia, but also in nuclei of tubular epithelia, in nuclei and cytoplasm of glomerular epithelia and mesangial cells, and in GBM. Conclusion: Our study suggested the presence of HBV infection and replication in glomerular cells and renal tubular epithelia, indicating an etiologic role of HBV in HBV-associated glomerulonephritis.

Objective To develop a HPLC method for the simultaneous content determination of three terpenoids inMelastoma dodecandrum Lour. (asiatic acid, betulinic acid, oleanolic acid).Methods The chromatographic column was set with waters SunFire C18 column (4.6 mm×150 mm, 5μm); the moving phase was acetonitrile -0.1%H3PO4; the column temperature was 30℃; the detection wavelength was 200 nm; the flow rate was 0.6 ml/min; and the sample volume was 25μl.Results A good linear relationship was observed in the range of 0.310-6.200μg (r=0.9999), 0.405-8.100μg (r=0.9999), 0.169-3.375μg (r=0.9998) for asiatic acid, betulinic acid, oleanolic acid respectively, with the average recovery rates of 102.08%, 101.81%, 102.22%. Conclusions The established method is convenient and sensitive, repeatability and stability, quality evaluation for Melastoma dodecandrum Lour.

Medical record writing may open up the clinical intern's clinical field of vision his,raise clinical thought,and improve his medical comprehensive ability.The clinical intern in medical record writing has the following questions:the delayedwriting,unstandard language and the unreal content and so on.Through the centralizing teaching,teachers'prompt counsel and interns'initiation and so on,clinical interns'medical record writing quality may be enhanced.

Objective To apply chaos characteristics to prediction of the unilateral mastication.Methods The paper calculated the Largest Lyapunov Exponent of the masseter muscle of some males and females with a method from small data sets,which then was processed by reusable two-factor analysis of variance.Results The results shows that the signal of the masseter muscle has chaos characteristics,the male's Largest Lyapunov Exponent is higher that the female's,and the chaos degree of the masseter muscle on both sides is consistent nearly.Conclusion The Largest Lyapunov Exponent can be used to characterize the signal of the masseter muscle.Comparative Analysis of the Largest Lyapunov Exponent on both sides can be used as reference when to predict and diagnose the unilateral mastication.

Objective To evaluate the clinical usefulness of MR imaging(MRI) and MR tomography angiography(MRTA) in demonstrating the presence and degree of neurovascular contact of the rostral ventrolateral medulla oblongata(RVLM) in root entry zone of the 9th and 10th cranial nerves in patients with essential hypertension(EHT) and normotensive health volunteers(NTHV).Methods(Patients with) EHT(group 1,n=100) and NTHV(group 2,n=88) underwent high-resolution(axial and coronal) brain stem MRI and MRTA.Images were interpreted consensually by tow radiologists who were blinded to the patients hypertensive status.Neurovascular contact was graded as vessel contact without RVLM deformity(gradeⅠ),clear vessel contact in continuity with the brain stem but without apparent associated deformity(graded Ⅱ), and displacement contact of the RVLM(graded Ⅲ).Results(Neurovascular) contact of RVLM was found in 52.0%(52/100) of EHT and in 43.2%(38/88) of NTHV(?~2=1.459,P=0.230).The compression rate(gradeⅠ—Ⅲ) and affected side(left or right) showed no statistically significant differences between the EHT and NTHV(?~2=0.879,P=0.350;?~2=0.238,(P=0.628);?~2=0.733,P=0.390).Conclusion Neurovascular contact is not more frequently seen in patients with EHT than in normotensive contact subject.This result does no support the hypothesis that neurovascular contact at the RVLM is an etiology of EHT.Furthermore,thin-slice(3 mm) MRI may not be a reliable good screening method for detecting patients with neurovascular contact.Therefore,MRI cannot aid patient selection among hypertension patients lacking symptomatic cranial neuralgias.

Cognition ; Cognition Disorders ; Humans ; Manganese ; Occupational Exposure

Objective To study the effects of high albumin, high glucose and low bovine serum on the phenotypic transformation of renal tubular epithelial cells. Methods The normal human kidney proximal tubular eel] line (HKC) was cultured for 30 days in the presence of high albumin (1.5 g/L), high glucose(25 mmol/L) and low bovine serum(2% ) . Morphological changes were observed by electronic microscopy. Immunohistochemistry stain was used to examine the expression of cytokeratin, vimentin, a-SMA, collagen Ⅰ and TGF-pl protein. Western blot was applied to further detect the process of collagen I protein expression, and in situ hybridization was used to examined the expression of collagen Ⅰ gene. Results Renal tubular epithelial cells cultured in high albumin, high glucose and low bovine serum showed obvious morphologic changes, including elongated shape, decrease of microvilli and mitochondria, and increase of rough endoplasmic reticulum under electronic microscopy. Immunohistochemistry stain revealed the reduction of cytokeratin, and enhancement of vimentin, ?-SMA, TGF-?1 and collagen Ⅰ. Western blot demonstrated that the expression of collagen Ⅰincreased in a time-dependent manner, and in situ hybridization showed that collagen type Ⅰ mRNA increased as well. Conclusion High albumin, high glucose and low bovine serum induce phenotypic transformation of renal tubular epithelial cells into mesanchymal cells.