With the method of fluid-filled stomach and intravenous administration of anisodaminal hydrochloridum(654-2), 94 cases of 11 gastroduodenal diseases including gastric cancer and others were examined with ultrasound. Comparison of the ultrasonography with the pathological changes was done. The finding rate of gastric foci which equal to or more than 5mm in diameter was 96.8%. The correct rate of diagnosis was 80.9%. The accuracy in differentiating early gastric cancer from advancing gastric cancer was 87.5%. The correct rate for judging invasive depth of stomach wall by gastric cancer was 82.9%. It can also show the distinct ultrasonic features for gastric submucosal diseases.

Humans ; Infant ; Milk, Human ; chemistry ; Paraquat ; poisoning ; Poisoning ; diagnosis

Objective To evaluate the clinical usefulness of MR imaging(MRI) and MR tomography angiography(MRTA) in demonstrating the presence and degree of neurovascular contact of the rostral ventrolateral medulla oblongata(RVLM) in root entry zone of the 9th and 10th cranial nerves in patients with essential hypertension(EHT) and normotensive health volunteers(NTHV).Methods(Patients with) EHT(group 1,n=100) and NTHV(group 2,n=88) underwent high-resolution(axial and coronal) brain stem MRI and MRTA.Images were interpreted consensually by tow radiologists who were blinded to the patients hypertensive status.Neurovascular contact was graded as vessel contact without RVLM deformity(gradeⅠ),clear vessel contact in continuity with the brain stem but without apparent associated deformity(graded Ⅱ), and displacement contact of the RVLM(graded Ⅲ).Results(Neurovascular) contact of RVLM was found in 52.0%(52/100) of EHT and in 43.2%(38/88) of NTHV(?~2=1.459,P=0.230).The compression rate(gradeⅠ—Ⅲ) and affected side(left or right) showed no statistically significant differences between the EHT and NTHV(?~2=0.879,P=0.350;?~2=0.238,(P=0.628);?~2=0.733,P=0.390).Conclusion Neurovascular contact is not more frequently seen in patients with EHT than in normotensive contact subject.This result does no support the hypothesis that neurovascular contact at the RVLM is an etiology of EHT.Furthermore,thin-slice(3 mm) MRI may not be a reliable good screening method for detecting patients with neurovascular contact.Therefore,MRI cannot aid patient selection among hypertension patients lacking symptomatic cranial neuralgias.

Objective: To detect the localization of HBV DNA in kidneys of HBV-associated glomerulonephritis, and investigate the pathogenesis of HBV-associated glomerulonephritis. Methods: 45 cases of renal biopsy specimens (38 cases had glomerular deposition of HBV antigens, 7 cases were negative for serologic and histologic HBV antigens) were examined for HBV DNA by in situ hybridization (ISH) and in situ PCR (IS-PCR). Results: The positive rate of HBV DNA in renal biopsy was 71% (27/38) in patients with glomerular deposition of HBV antigens. HBV DNA was found in 19 cases (19/26, 73%) of HBV-associated glomerulonephritis; and in 8 cases (8/12, 67%) of IgA nephropathy and lupus nephritis with glomerular deposition of HBV antigens. HBV DNA was detected mainly in the cytoplasm of tubular epithelia with ISH; IS-PCR showed that HBV DNA was localized not only in cytoplasm of tubular epithelia, but also in nuclei of tubular epithelia, in nuclei and cytoplasm of glomerular epithelia and mesangial cells, and in GBM. Conclusion: Our study suggested the presence of HBV infection and replication in glomerular cells and renal tubular epithelia, indicating an etiologic role of HBV in HBV-associated glomerulonephritis.

Objective To apply chaos characteristics to prediction of the unilateral mastication.Methods The paper calculated the Largest Lyapunov Exponent of the masseter muscle of some males and females with a method from small data sets,which then was processed by reusable two-factor analysis of variance.Results The results shows that the signal of the masseter muscle has chaos characteristics,the male's Largest Lyapunov Exponent is higher that the female's,and the chaos degree of the masseter muscle on both sides is consistent nearly.Conclusion The Largest Lyapunov Exponent can be used to characterize the signal of the masseter muscle.Comparative Analysis of the Largest Lyapunov Exponent on both sides can be used as reference when to predict and diagnose the unilateral mastication.

Objective To develop a HPLC method for the simultaneous content determination of three terpenoids inMelastoma dodecandrum Lour. (asiatic acid, betulinic acid, oleanolic acid).Methods The chromatographic column was set with waters SunFire C18 column (4.6 mm×150 mm, 5μm); the moving phase was acetonitrile -0.1%H3PO4; the column temperature was 30℃; the detection wavelength was 200 nm; the flow rate was 0.6 ml/min; and the sample volume was 25μl.Results A good linear relationship was observed in the range of 0.310-6.200μg (r=0.9999), 0.405-8.100μg (r=0.9999), 0.169-3.375μg (r=0.9998) for asiatic acid, betulinic acid, oleanolic acid respectively, with the average recovery rates of 102.08%, 101.81%, 102.22%. Conclusions The established method is convenient and sensitive, repeatability and stability, quality evaluation for Melastoma dodecandrum Lour.

Medical record writing may open up the clinical intern's clinical field of vision his,raise clinical thought,and improve his medical comprehensive ability.The clinical intern in medical record writing has the following questions:the delayedwriting,unstandard language and the unreal content and so on.Through the centralizing teaching,teachers'prompt counsel and interns'initiation and so on,clinical interns'medical record writing quality may be enhanced.

Objective To investigate the prognosis of two decompression approaches for cervical spondylotic myelopathy. Methods 86 cases were divided into two groups. 40 cases were underwent decompression by anterior decompression, fusion and internal fixation with titanium screws and plate and 46 cases underwent posterior single opendoor laminoplasty. To investigate the prognosis of two decompression approaches for single compressive segment, two compressive segments, three compressive segments and four compressive segments. Results All cases were followed up for 20 ～ 73 months with an average of 43 months. The mean JOA recovery rate was significant different between single compressive segment group (P ＜ 0.05), and no significant difference between two compressive segments and three compressive segments(P ＞ 0.05), and significant difference between four compressive segments groups (P ＜0.05). Conclusion For single compressive segment, the anterior surgery has a good surgical result,for two or three compressive segments, anterior and posterior surgery had same effect, for the four compressive segments, posterior surgical effect was good.

Purpose To investigate the expression of gelsolin-like actin-capping protein (CapG) in gastric cancer and adjacent normal gastric tissues and its relationship with clinicopathological features and prognosis.Methods The immunohistochemical (IHC) assay was performed to detect the expression of CapG protein in 125 specimens of gastric cancer (study group) and 30 specimens of matched adjacent normal gastric tissues (control group).No patients had received chemotherapy or radiotherapy before surgery.Immunostaining was scored semiquantitatively by two independent observers who were blinded to the patients' outcome and other clinicopathological parameters.The correlation of the expression of CapG with clinicopathological characteristics and prognosis was analyzed.Results Cytoplasmic and nuclear CapG protein staining was detected in all gastric cancer tissues.CapG protein expression was absent or low in matched adjacent normal gastric tissues but significantly higher in gastric cancer tissues.Expression of CapG in the tumor was not significantly associated with gender,age,tumor size,tumor location (P ＞ 0.05).However,elevated CapG expression was strongly correlated with the depth of invasion (P =0.044),lymph node metastasis (P =0.026),distant metastasis (P =0.001),and AJCC stage (P =0.012).Significant poorer overall survival was observed in the high CapG expression group compared with that of the low CapG expression group (P ＜0.001).Multivariate analysis showed overall survival correlated with the patients' tumor location,lymph node metastasis,distant metastasis,AJCC stage and the expression of CapG protein.Conclusion CapG is highly expressed in gastric cancer.Tumors with CapG up-regulation tend to have poorer prognosis,suggesting overexpression of CapG may contribute to the prediction of poorer prognosis and may be a potential therapeutic target for gastric cancer.

Objective　To display efficient folding hDAF on the surface of yeast. Methods　The hDAF open reading frame was cloned by PCR from DAF- pBluescript M13-（Amp＋）plasmid， then subcloned into the yeast surface displayed vector pYD1.The recombinant vector was transformed into yeast cells EBY100.Flow cytometric analysis was carried out to evaluate direct binding of anti-DAF mAbs onto the surface of yeast cells displaying DAF. Results　Three mAbs against DAF different epitopes could bind onto DAF displayed on the surface of yeast.Conclusion　Efficient folding DAF can be displayed on the surface of yeast potentially leading to the development of novel applications involving DAF yeast display.