Objective To prepare the ephedrine chitosan-modified liposomes gels, detect the particle diameter and the entrapment efficiency, and investigate the transdermal diffusion characteristics in vitro. Methods Ephedrine liposomes suspensions were prepared using thin film evaporation and slowly dropped by 0.35% chitosan solution with pH values 5-6 in order to prepare the chitosan-modified liposomes gels. The particle diameter of the modified liposomes was detected by Laser Scattering Particle Size Distribution Analyzer. The entrapment efficiency was determined by reverse dialysis method with HPLC. Using Franz Diffusion Cell to investigate transdermal diffusion characteristics of the modified liposomes gels in vitro. Results The mean particle diameter of the modified liposome prepared was from 1 400 to 1 900 nm. The average entrapment efficiency of the modified liposomes was up to (41 ± 0.94)%. Ephedrine in the modified liposomes could penetrate rat skin. The results of the transdermal diffusion test showed the cumulative penetration amount of the ephedrine chitosan-modified liposomes gels was lower than that in the ephedrine solutions with time variation. Conclusion This preparatiom is prepared with simple technology and stable character. The entrapment efficiency of the ephedrine is higher and the assay method is simple and accurate. The penetrating rate of ephedrine is slow and the drug releasing is stable.

A lipopeptide compound was isolated from the culture of Bacillus subtilis HSO121 by methods of acidic precipitation, solvent extract, fractional precipitation, adsorption and prepared thin-layer chromatography; and its molecular structure was determined by by ninhydrin assay and IR methods following the Amino analysis, MS-MS and ESI-MS. It shows that the isolated lipopeptide consists of two homologues with molecular mass 1,022D and 1,036D and bearing a cyclic structure with the amino acid sequence Leu-Leu-Asp-Val-Leu-Leu-Glu in the peptide chain, which indicates that the isolated lipopeptide falls into the analogs of surfactin.

Objective: To study the relationship among shame, persona li ty and mental health, and to establish a structural model of them Meth o d: Structural equation modeling was used for the data from 178 college s tudents (including 86 male and 92 female, mean age 21), who were tested with SCL -90, EPQ-R Short Scale (EPQ-RS)and shame scale Result: A theor etical model was established for the relationship of personality, shame and ment al health It wa s also confirmed through model comparison that shame has a part-mediating role b etween personality and mental health

Objective: To study social anxiety proneness in college students and factors having influence on it Method: 200 college students were assessed with Social Anxiety Inventory, Shame-Proneness Scale, EQQ-short form, Coping Style Questionnaire, Beck Depression Scale, and Trait Anxiety Scale Result: Social anxiety of college students was influenced by shame-proneness, psychoticism and extroversion of personality Students with high social anxiety proneness were also more easily ashamed by their own behaviors Lack of negative coping styles also contributed to their proneness Low social anxiety proneness could be explained partially by three factors: psychoticism and extroversion personality, self-appraised trait anxiety Conclusion: Shame-proneness, psychoticism and extroversion personality are main factors having influence on social anxiety proneness Positive self-evaluation and coping style are helpful in reducing social anxiety

An applied anatomical study on the blood supply of the flexor tendon was conducted in the upper extremities of fresh cadaver by means of arterial injection, transparence, maerodissection and histological preparation.The flexor digital tendon can be divided into two parts: non-synovial membrane part and synovial membrane part. The intrinsic vaseularization of non-synovial membrane part of the tendon which was wrapped in paratenon was major in the form of the longitudinal vascular bundles, while the transverse anastomotie branches were short and sparse. The vascularity of this part was simple and uniformly distributed. The intrinsic vascularity of the synovial part possessed distinct characteristics of segmentality and partiality. In the synovial sheath, the blood vessels distributed only onto the dorsal aspect, while the velar 1/3 to 1/2 of the tendon was devoid of vessels. The profundus and superfieialis tendons formed an avascular zone at the proximal interphalangeal and metacarpophalangeal joint separately. It was considered that the difference of the vascularization of tendon might be related to the mechanical force to which the tendon was subjected. The nutrition of tendon was discussed and the selection of tendon graft in the operation was suggested.

Objective To investigate the effects of dexamethasone on the growth of cervical cancer induced by HeLa cells transplanted into BALB/c mice. Methods HeLa cells were inoculated subcutaneously into BALB/c mice. Dexamethasone (15 mg?kg-1?d-1) was injected intraperitoneally from the 6th or the 16th day after tumor inoculation for 10 days continuously. Diameter of tumors was measured every two or three days. Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL. Results Dexamethasone repressed the growth of cervical cancer and induced tumor cell apoptosis, but the tumor volumes were of statistical difference between the two groups of different injection start time (P

Objective To investigate the effects of over physiological dose of dexamethasone on cervical carcinoma cell xenografted tumors in nude mouse.Methods HeLa cells were inoculated subcutaneously in BALB/c female mice aged 5-6 weeks.Over physiological dose of dexamethasone(62.5 mg?kg-1?d-1) was injected into the peritoneum from the 16th day after inoculation for 10 d.Diameters of tumor were measured every two or three days.The volumes and the inhibition rates of tumor were calculated.Apoptotic bodies were observed by transmission electron microscopy and counted by TUNEL.VEGF and TNF-? were detected by immunohistochemistry.Results In vivo,over physiological dose of dexamethasone can repress the growth of tumor and induce cell apoptosis.Compared with the control group,the tumor sizes were significantly smaller(P0.05).Conclusion Over physiological dose of dexamethasone can repress cervical tumor growth in vivo.This action may be related to apoptosis and VEGF,but have no relationship with TNF-?.

AIM: To select the macroresin used for purification of total saponins of Polygala Fallax Hemsl. METHODS: The adsorption and desorption capability of five different macroresins LSA-7,LSA-10,LSA-30,D-101A and HPD-100 in static state were studied by UV. RESULTS: nonpolar LSA-30 and HPD-100 macroporus resin had the optimum comprehensive properties of adsorption and desorption. CONCLUSION: The experimental results provide the basis for selection of macroporus resins used to purify total saponins of Polygala Fallax Hemsl.

BACKGROUND:Intertrochanteric fracture is one of the common complications in patients with senile osteoporosis.Internal fixation is a common method in clinical therapy.Different internal fixators are selected in different studies.However,there are few studies on influential factors for outcomes of internal fixation at present.OBJECTIVE:To investigate related influential factors for outcomes of internal fixation in elderly patients with osteoporotic intertrochanteric fracture.METHODS:A total of 86 elderly patients with osteoporotic intertrochanteric fracture,who were treated in the Guanghua Hospital from May 2011 to May 2014,were enroled in this study.They were divided into three groups according to different manners of fixation.There were dynamic hip screw group (n=34),proximal intramedulary nail group (n=41) and proximal femoral compression plate group (n=11).Rate of fixation failure was compared among the three groups.The relevant factors affecting fixation effects were analyzed using univariate and multivariate analysis.RESULTS AND CONCLUSION:Internal fixation failure was detected in 5 patients (15％) in the dynamic hip screw group,11 patients (26％) in the proximal intramedulary nail group,and 4 patients (36％) in the proximal femoral compression plate group.Results of univariate analysis revealed that Evanse genotyping of patients,whether fractures were found on outer arm side,degree of osteoporosis,quality of reduction,steeple distance,whether combined with internal medicine diseases and selection of fixator were associated with internal fixation effects (P＜ 0.05).However,the internal fixation effects were not correlated with gender,age,hospital stay and ambulation time (P＞ 0.05).Multivariate analysis results demonstrated that unstable fractures,severe osteoporosis,fractures of the lateral arm,medical ilness,steeple distance ＞ 25 mm,and different internal fixators are independent factors affecting internal fixation effects in patients.

Objective To compare the effect of two different dialysis modalities, hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) on insulin resistance in patients with adult end-stage renal disease (ESRD), and to identify the possible predictive factors for insulin resistance. Methods Fifteen non-diabetic patients with ESRD (ESRD group) were selected. Eight patients were treated with HD (HD group), and 7 patients were treated with CAPD (CAPD group). The insulin inhibition was examined by hyper insulin-euglycemic glucose clamp technique before and after dialysis treatment, and the glucose disposal rate (GDR) was used as an index of insulin sensitivity during the clamp technique. Meanwhile, 8 healthy controls were selected as control group. The biochemical parameters which might be associated with insulin resistance were determined by multiple linear regression. Results The GDR in control group was (9.93 ± 1.33) mg/(kg · min), in ESRD group was (6.44 ± 1.76) mg/(kg·min), and there was statistical difference (P<0.05). The GDR in HD group after treatment was increased from (6.53 ± 1.84) mg/(kg · min) to (9.74 ± 2.88) mg/(kg · min), and there was statistical difference (P<0.01). The GDR in CAPD group after treatment was increased from (6.35 ± 1.65) mg/(kg·min) to (8.18 ± 1.76) mg/(kg·min), and there was statistical difference (P<0.05). Multiple linear regression result showed that the levels of urea nitrogen, hematocrit and bicarbonate were significant predictive factors in insulin resistance (P<0.05). Conclusions CAPD and HD therapy can improve insulin resistance in adult patients with ESRD.