We have studied the induction of mice spleen suppressor cell activity by Trypterygium Wilfordii Hook f. both in vivo and in vitro. We detected the suppressor cell activity in mice which were administrated with the drug for different days & found that after giving the drug for 5 or 9 days, the suppressor cell activity elevated remarkly, but for 12 & 15 days, the suppressor cell activity varied indefinitely, some were higher & some were lower. The drug inhibited the response of spleen cell to Con A too. But the release of inhibition on Con A responsibility was earlier than the recovery of elevated suppressor cell activity. Cocultured with the drug, slight promotion on cell proliferation was observed. The normal spleen cells cocultured with the drug for two days suppressed the normal spleen cell proliferation stimulated by Con A, the suppressive rate was comparable to that of Con A induced suppressor celll.

Herpes simplex keratitis (HSK) is the first inflammatory cause for uniocular blindness.The clinical inspection methods commonly used are limited to general observation,while in vivo confocal microscopy (IVCM) can provide details of cellular changes in an intravital HSK.IVCM not only gives more clinical evidence for pathology studying of HSK,but also serves as a new technology for the whole progress of HSK epidemiology.This article reviewed current researches of applying IVCM for HSK clinical observation and summarized these latest results.Morphological changes of each layer of the cornea,the specificity of these changes and clinical application of IVCM had been discussed in this article.

According to the principles of cognitive psychology,the rational-emotional therapy has effectives on patients with emotional disorder in changing activating events,transferring their belief and then correcting their emotional experience.The content of emotional therapy and psychotherapy in Inner Canon of Huangdi are:joy prevailing over melancholy,anger prevailing over anxiety,fear prevailing over joy,sorrow prevailing over anger,anxiety prevailing over fear,and so on.It has practical clinic signifi cance.

OBJECTIVE:To study the method of preparing PEG-SOD enteric-coated capsuls and quality standard.ME_ THODS:Orthogonal design was used to select the optimal conditions for preparing PEG-SOD enteric-coated capsuls and the relevant quality standard of PEG-SOD enteric-coated capsuls was established according to the China Pharmacopoeia(2000). RESULTS:The optimal conditions were as follows:the content of PEG-SOD per capsule was40000IU;the weight ratio of stabilizer lactose was2.5%;filler was carboxymethyl starch;drying process was at60℃for20min.The preparation was ac?corded with the relevant quality standard.CONCLUSION:The technic of preparation is feasible,the method of quality control is reliable and the quality of preparation is stable.

Objective To evaluate the application efficacy of responsibility system for holistic nursing system in high-quality nursing service in the department of respiratory medicine.Methods Department of respiratory medicine unit which was the quality nursing service demonstrated unit in our hospital was selected.The nursing responsibility-bound grouping system was first performed and the nurse-incharge would provide the patients with whole and continuous nursing service.Before and 6 months after the application,the nursing job satisfaction was conducted and the nursing grouping system satisfaction was also evaluated among nurses and doctors.Results Six months after the application of nursing responsibility-bound grouping system,the patient satisfaction with nursing job was raised obviously in the unit.Conclusions The application of responsibility-bound grouping system can not only promote the activity but also mobilize the enthusiasm of nurses,and then high-quality nursing service was resulted.So the service system was worthy of clinical application.

Objective To observe the effect of carvedilol and motoprolol on the weight of cardiac muscle in patients with hypertensive heart disease.Methods One hundred patients with hypertensive heart disease were divided into two groups by random number and treated with carvedilol(n =54) and motoprolol(n =46) respectively for 12 months.Data were recorded (left ventricular end-diastolic dimension (LVEDD),left ventricular end-systolic dimension (LVESD),left ventricular ejection fraction (LVEF),interventricular septal thickness (IVST),and left ventricular posterior wall thickness (LVPWT)) before and after the treatment.Left ventricular muscle mass(LVW),left ventricular mass index(LVWI) were calculated.Results After treatment,both groups improved with declined LVEDD (motoprolol group:(43.60 ± 4.43) mm vs.(46.70 ± 3.21) mm,t =18.143,P ＜ 0.01 ; carvedilol group:(42.50 ± 2.56) mm vs.(46.5 ± 3.18) mm,t =18.232,P ＜ 0.01),thinner IVST (motoprolol group:(9.68 ± 1.65) mm vs.(12.01 ± 1.56) mm,t =12.785,P ＜ 0.01 ; carvedilol group:(9.05 ± 1.04) mm vs.(11.59 ± 1.54) mm,t =7.865,P ＜ 0.01),and increased LVEF (motoprolol group:(52.89 ± 8.78)％ vs.(50.23 ± 7.88)％; carvedilol group:(54.65 ± 8.87％)％ vs.(50.22 ±7.89)％).In the carvedilol treatment group,LVMI significantly (t =4.987,P ＜ 0.01) declined from (133.75 ±25.89) g/m2 to (109.25 ± 22.53) g/m2.In the motoprolol treatment group,LVMI declined from (134.76 ± 25.87) g/m2 to (119.78 ± 23.65.53) g/m2 (t =5.689,P ＜ 0.01).After the treatment,each index of carvedilol group improved significantly compared with metoprolol group (P ＜ 0.05).Conclusion Both medications are able to reduce the weight of cardiac muscle of patients with hypertensive heart disease,and carvedilol is better than motoprolol.

Transcription factorhypoxia inducible factor -1(HIF-1)plays a key role in cell adaption to hypoxia(from 21%O2 to 1%O2).Researches have shown that HIF -1 is closely related to occurrence and de-velopment in many malignant cancers .In this paper,we review the effect of HIF -1 in tumor growth,metastasis, apoptosis and angiogenesis .

Objective To investigate the effect of Tegafur, Gimeracil and Oteracil Potassium Capsules ( TS-1 ) on peripheral blood tissue polypeptide antigen (TPS), soluble MHC class I chain-related molecules A (sMICA), hypoxia-inducible factor-1 (HIF-1α) and human epidermal growth factor receptor 2 ( Her-2 ) in the treatment of patients with advanced cervical cancer.Methods 80 cases with advanced cervical cancer were selected and divided into two groups, 40 cases in the control group were treated with routine clinical therapy, 40 cases in the experimental group were treated with TS-1.Tumor markers, TPS,sMICA,HIF-1αand Her-2 were compared before and after the treatment.Results Compared with the control group, the levels of tumor antigen TA-4,carcinoembryonic antigen (CEA) and squamous cell carcinoma antigen (SCCA) in the experimental group were lower (P<0.05); the TPS, HIF-1, sMICA and Her-2 were lower (P<0.05), and the total effective rate in the treatment group was higher (P<0.05).Conclusion TS-1 has a obvious clinical efficacy in the treatment of advanced cervical cancer.It is speculated that the possible mechanism may reduce the serum tumor marker levels via reducing the TPS and SMICA, HIF-1 alpha, HER-2 levels.

Nitric oxide (NO) is the most important known endogenous vasodilator factor. The competitive inhibitor asymmetric dimethylarginine (ADMA) of endothelial nitric oxide synthase (NOS) inhibits NO synthesis, which makes NO/NOS pathway to be obstructed and the reduction of NO synthesis. The studies in recent years have suggested that ADMA is as-sociated with the occurrence and development of diabetes with cerebrovascular disease. It has provided new insights into further clarifying the mechanism of the disease.

Objective:To research on the radio frequency ablation (RFA) combined with the clinical effect of chemotherapy in patients with advanced non small cell lung cancer and lung metastasis. Methods:From 2011 May to 2012 in our hospital 06 months 60 cases of metastatic non-small cell lung cancer and lung cancer patients, divided into radio frequency ablation(RFA) combined with chemotherapy group of 30 cases and a simple chemotherapy group 30 cases, referred to as the treatment group and the control group. To observe the change of tumor volume in patients with poor survival rate, years, tumor recurrence rate, and postoperative complications. Results:Three months after treatment in treatment group tumor recession(CR) in 5 cases(16.7%), part of the recession(PRa) 18(60%), partial mild recession(PRb) 6(20%), no degradation(NR), 1(3.3%), tumor size change significantly better than the control group. The treatment group patients 12 months survival rate was 86.7%, higher than that of the control group(70%). The treatment group of 12 months of tumor recurrence rate of 6.7%, lower than that of the control group(26.7%). No obvious adverse complications of the treatment group of 30 patients after operation. Conclusion:Radiofrequency hyperthermia ablation combined with chemotherapy in treatment of advanced non small cell lung cancer and metastatic lung cancer clinical effect is obvious.