Objective To investigate the causes of failure of manually rotating fetal head technology and its delivery outcomes.Methods One hundred and Ninety-eight cases with persistent occipito transverse position or persistent occipitoposterior position admitted to our hospital form January 2008 to December 2010 were enrolled as our subjects.According to the result of manually rotating fetal head technology,these patients were divided into the success group (126 cases) and the failure group (72 cases).The information related to delivery was recorded.Results The proportion of fetal weight ≥ 3500 g in the failure group was 76.4％ (55/72),higher than that of the success group(31.7％ (40/126)),and there has statistic significant difference (x2 =30.177,P =0.001).The proportion of uterine inertia in the failure group(58.3％ (42/72)) was higher than that of the success group(38.1％ (48/126)),and there has statistic significant difference (x2 =7.569,P =0.006).The proportion of critical or low-grade narrow birth canal in the failure group(38.9％ (28/72)) was significantly higher than that of the success group (23.8％ (30/126)),and there has statistic significant difference (x2 =5.030,P =0.002).The proportion of the improper time for manually rotating fetal head (opening of cervix was less than 6 cm,and fetal head was above ischial spine and opening of cervix was 8-10 cm,fetal head reach to more than 2 cm lower of ischial spine) in the failure group was 61.1％ (44/72),which was significantly higher than that of the success group (38.9 ％ (49/126)),and there has statistic significant difference (x2 =9.084,P =0.003).The proportion of maternal and neonatal complications (postpartum hemorrhage,puerperal morbidity,fetal distress,neonatal asphyxia) in the failure group were significantly higher than those of the success group (x2 =9.586,P =0.002 ; x2 =9.334,P =0.002 ; x2 =5.910,P =0.015 ; x2 =5.240,P =0.022).The proportion of cesarean section in the failure group(72.2％ (52/72)) was significantly higher than that of the success group(34.1％ (43/126)),and there has statistic significant difference(x2 =26.641,P =0.001).Conclusion The manually rotating fetal head technology can transform from dystocia to normal delivery and decrease the rate of cesarean section.It is necessary to prevent and deal with the failure causes of manually rotating fetal head technology.And it must be cautious to perform the follow-up correction and trial production after the failure of previous correction.

Asian Continental Ancestry Group ; China ; Humans ; Laboratories ; organization & administration ; Measles ; prevention & control ; Measles Vaccine ; administration & dosage

Objective To evaluate the efficacy of MRCP in the determination of common bile duct (CBD) exploration in gallstone pancreatitis(GP). Methods The clinical data of the patients who underwent CBD exploration due to gallstone pancreatitis during two different periods were retrospectively reviewed. Between 1995 and 1999,46 patients with gallstone pancreatitis underwent CBD explorations based on the preoperative ultrasonographic examination of stones and dilatation of CBD . Between 2000 to 2002,the explorations were carried out in 30 patients based on MRCP findings. Results Negative exploration occurred in 28 patients (60.8%) during the first period and 7 patients (23.3%) during the second period. The difference of negative CBD exploration rate between the two groups was statistical significance(P

The blood brain barrier(BBB) is a barrier system between the blood and brain tissue,which can maintain relatively stable of internal environment in the central nervous system (CNS).Cerebral hypoxiaischemia and other conditions can lead to the change of the permeability of BBB.This paper reviews the latest research for the structural basis and function of BBB,and for the scope,time and mechanism of the change of BBB structure and function during hypoxic-ischemic brain injury.This theory in the regulation of BBB may play an important role in the treatment of hypoxic-ischemic brain injury and underlying diseases of CNS.

Objective To examine the relationships among measures of gait recovery and community integra-tion index in people with brain injury.Methods 142 brain injured patients were chosen in this study.Community Balance and Mobility Scale,Dynamic Gait Index,Ten-Meter Walk Test for gait speed,and the Community Integration Questionnaire (CIQ) was measured.Results Average walk speed was 0.31-5.30 (1.36 ±0.88)m/s;DGI score was 5-24 (18.6 ±5.44) points;CMBS score was 0 -96 (54.1 ±25.3) points;CIQ subscale 6 -29 (16.2 ± 5.1)points,CIQ family subscale was 0-10 (4.8 ±3.0)points;CIQ society subscale was 4-12(8.2 ±2.1)points;CIQ productivity subscale was 0-7(3.8 ±2.2).The correlation between age and Ten-Meter Walk Test was nega-tive significantly (r=-0.50,P<0.05).All 3 balance/gait measures correlated significantly with the CIQ Productiv-ity subscale (r=0.35-0.58,P<0.05).Conclusion The ability of people with brain injury to engage in work/school/volunteer activity may be reduced by impairments in balance and mobility.

Objective To study the adverse effect of omethoate on DNA in mouse sperm cells in vivo. Single cell gel electrophoresiss (SCGE) or comet assay was used to observe the damage of DNA. Methods 40 male kunming mice were randomly divided into 4 groups and were treated with omethoate by gavage at 0 mg/kg, 0.5 mg/kg, 1 mg/kg and 2 mg/kg respectively, once a day for 35 consecutive days. Results The movement and density of sperms decreased as the dose of omethoate increased, the length of comet tails in group of 1 mg/kg and group of 2 mg/kg was much longer than that in the control, the other indexes of comet assay also showed significant DNA damage. Conclusion Omethoate exposure can induce DNA damage in the sperm cells of male mice.

Purpose:To study LPS induced iNOS mRNA expression and NO production by murine peritoneal macrophages, and their relationship with cytotoxic function of activated macrophages.Methods:LPS induced iNOS mRNA expression, production of NO and effects of activated macrophages on anti tumor were investigated separately by RT PCR, nitrate reductase method and 3HTdR incorporation.Results:LPS can upregulates iNOS mRNA expression in a dose dependent manner. L NIL(specific inhibitors of iNOS) pretreatment significantly reduced cytotoxic effect on the proliferation of HL 60 cells, but had little effect on the K562 tumor target cells on condition that NO production is sufficiently inhibited. Conclusions:LPS can effectively induce iNOS mRNA expression and NO production in murine peritoneal macrophages. NO is one of the important effector molecules in unspecific immunity of activated macrophages, but it plays different roles in cytotoxic effects of macrophages on different target tumor cells.

Objective:To study the risk factors and treatment of lymphatic fistulas after the radical operation of gastric cancer.Methods:Observed the clinical data of 631 cases after the radical operation of gastric cancer that had been received in our hospital from February 1998 to February 2008.Multiple factor analysis with logistic regression was performed for the risk factors.Results:The level of post-operative serum albumin on the first day and Ultrasonically activated shears are protective facrors against lymphatic fistulas(P

OBJECTIVE:To study the clinical pharmacokinetics of Midazolam.METHODS:Fourteen patients undergoing general anesthesia with Midazolam in combination with remifentanil and isoflurane were enrolled in our study.The midazolam was administered by constant-rate infusion of 20 ?g?kg-1?min-1 for 20 min.The sampling of radial artery blood samples(3 mL/sample)were schedule before the infusion and at 1,3,5,7,10,15 and 20 min after the initiation of infusion and at 5,15,30,45 min and 1,2,4,6,12,18,24 h following the completion of midazolam.The plasma midazolam concentrations were determined by RP-HPLC.The pharmacokinetic analysis was performed using DAS2.1.1 program.RESULTS:The concentration-time curves of midazolam were best described by a simple three-compartment open model in which the weight coefficient was 1 and the typical pharmacokinetics parameters of midazolam were as follows:t1/2?=9.584 2 min,t1/2?=85.367 7 min,t1/2?=339.736 5 min,V1(the distribution volume of central compartment)=0.182 1 L?kg-1,CL=119.434 4 mL?h-1?kg-1,K10=0.045 8 min-1,K12=0.086 3 min-1,K21=0.017 5 min-1,K13=0.013 3 min-1,K31=0.002 4 min-1.CONCLUSION:t1/2?,t1/2?,t1/2? and CL were in line with those reported in the literature abroad,but V1 is on the lower side and it is easy for the medication to reach a balance.However,the rapid clearance of midazolam is more in line with the pharmacokinetic parameters of the Chinese subjects.

Objective To construct pEGFP-N1-TGF-β1 recombinant plasmid and transfect it into primary cultured neonatal piglet type Ⅱ alveolar epithelium cell (AEC-Ⅱ) by using lipofectamine 2000,in order to provide basis of methodology for producing recombinant plasmids for transplantation of transfected AEC-Ⅱ into ALI/ARDS animal model lungs.Methods PCR primers were designed to amplify the human TGF-β1 cDNA fragment from plasmid.XhoI and EcoRI were used for double digesting the empty plasmid pEGFP-N1 and cDNA fragment of human TGF-β1.Then the products of double enzyme digestion by using T4 DNA ligase were connected and transformed into DH5α and cultured over night for 16 hours.The structure of recombinant plasmid was identified by using PCR and base sequencing to verify the correctness of pEGFP-N1-TGF-β1 recombinant plasmid.It was then transfected into primarily cultured AEC-Ⅱ by lipofectamine2000 mediated transfection and cultured for another 48 hour.Plasmid DNA (pEGFP-N1-TGF-β1 recombinant plasmid) and lipofectamine 2000 were added into serum-free DMEM respectively,then DNA suspension and Lipofectamine 2000 suspension were blended together and added into cells.After 24-48 hours later,the expression level of enhanced green fluorescent protein (EGFP) was evaluated under fluorescence microscope.Results The structure of vector was verified as pEGFP-N1-TGF-β1 recombinant plasmid by using PCR and base sequencing.Green fluorescence found in some cells showed that the pEGFP-N1-TGF-β1 recombinant plasmids had been successfully transfected into primary cultured AEC-Ⅱ,however,the transfection efficiency still need tobe further improved such as repeating the transfection procedure once again or using adenovirus mediated transfection method to improve the efficiceny and to transplant the cells into animal lungs eventually.Conclusions pEGFP-N1-TGF-β1 recombinant plasmid was successfully constructed and,for the first time,transfected into primarily cultured AEC-Ⅱ of newborn piglets.This established method should be useful for investigation of therapeutic effect and outcomes of lung with experimental acute lung injury.