Objective To investigate the causes of failure of manually rotating fetal head technology and its delivery outcomes.Methods One hundred and Ninety-eight cases with persistent occipito transverse position or persistent occipitoposterior position admitted to our hospital form January 2008 to December 2010 were enrolled as our subjects.According to the result of manually rotating fetal head technology,these patients were divided into the success group (126 cases) and the failure group (72 cases).The information related to delivery was recorded.Results The proportion of fetal weight ≥ 3500 g in the failure group was 76.4％ (55/72),higher than that of the success group(31.7％ (40/126)),and there has statistic significant difference (x2 =30.177,P =0.001).The proportion of uterine inertia in the failure group(58.3％ (42/72)) was higher than that of the success group(38.1％ (48/126)),and there has statistic significant difference (x2 =7.569,P =0.006).The proportion of critical or low-grade narrow birth canal in the failure group(38.9％ (28/72)) was significantly higher than that of the success group (23.8％ (30/126)),and there has statistic significant difference (x2 =5.030,P =0.002).The proportion of the improper time for manually rotating fetal head (opening of cervix was less than 6 cm,and fetal head was above ischial spine and opening of cervix was 8-10 cm,fetal head reach to more than 2 cm lower of ischial spine) in the failure group was 61.1％ (44/72),which was significantly higher than that of the success group (38.9 ％ (49/126)),and there has statistic significant difference (x2 =9.084,P =0.003).The proportion of maternal and neonatal complications (postpartum hemorrhage,puerperal morbidity,fetal distress,neonatal asphyxia) in the failure group were significantly higher than those of the success group (x2 =9.586,P =0.002 ; x2 =9.334,P =0.002 ; x2 =5.910,P =0.015 ; x2 =5.240,P =0.022).The proportion of cesarean section in the failure group(72.2％ (52/72)) was significantly higher than that of the success group(34.1％ (43/126)),and there has statistic significant difference(x2 =26.641,P =0.001).Conclusion The manually rotating fetal head technology can transform from dystocia to normal delivery and decrease the rate of cesarean section.It is necessary to prevent and deal with the failure causes of manually rotating fetal head technology.And it must be cautious to perform the follow-up correction and trial production after the failure of previous correction.

Objective To construct pEGFP-N1-TGF-β1 recombinant plasmid and transfect it into primary cultured neonatal piglet type Ⅱ alveolar epithelium cell (AEC-Ⅱ) by using lipofectamine 2000,in order to provide basis of methodology for producing recombinant plasmids for transplantation of transfected AEC-Ⅱ into ALI/ARDS animal model lungs.Methods PCR primers were designed to amplify the human TGF-β1 cDNA fragment from plasmid.XhoI and EcoRI were used for double digesting the empty plasmid pEGFP-N1 and cDNA fragment of human TGF-β1.Then the products of double enzyme digestion by using T4 DNA ligase were connected and transformed into DH5α and cultured over night for 16 hours.The structure of recombinant plasmid was identified by using PCR and base sequencing to verify the correctness of pEGFP-N1-TGF-β1 recombinant plasmid.It was then transfected into primarily cultured AEC-Ⅱ by lipofectamine2000 mediated transfection and cultured for another 48 hour.Plasmid DNA (pEGFP-N1-TGF-β1 recombinant plasmid) and lipofectamine 2000 were added into serum-free DMEM respectively,then DNA suspension and Lipofectamine 2000 suspension were blended together and added into cells.After 24-48 hours later,the expression level of enhanced green fluorescent protein (EGFP) was evaluated under fluorescence microscope.Results The structure of vector was verified as pEGFP-N1-TGF-β1 recombinant plasmid by using PCR and base sequencing.Green fluorescence found in some cells showed that the pEGFP-N1-TGF-β1 recombinant plasmids had been successfully transfected into primary cultured AEC-Ⅱ,however,the transfection efficiency still need tobe further improved such as repeating the transfection procedure once again or using adenovirus mediated transfection method to improve the efficiceny and to transplant the cells into animal lungs eventually.Conclusions pEGFP-N1-TGF-β1 recombinant plasmid was successfully constructed and,for the first time,transfected into primarily cultured AEC-Ⅱ of newborn piglets.This established method should be useful for investigation of therapeutic effect and outcomes of lung with experimental acute lung injury.

Objective:To design a new rehabilitation system for cerebral apoplexy patients, and help patients complete rehabilitation treatment on the bed.Methods: Design the basic structure of the system through mechanical technology, make the realization of the function by using circuit and wireless control system, global-eye system was embedded in it by using the principle of the 3G to 4G intelligent technology for improving the humanized design of the system. Results:Through application in physical therapy, nursing, clinical rehabilitation, and clinical therapy in hospitals, it achieved good economic and social benefits, got the recognition of patients, clinicians and rehabilitation therapists. Conclusion: It can be used for the treatment of cerebral apoplexy, reduce medical expenditure, save amount of social resource and improve the work efficiency of medical staff.

Objective To investigate the effect of medication guide on IL-17,NO,IL-8 and the respiratory function in patients with COPD,and to provide evidence for the prevention and treatment of COPD.Methods From January 2014 to December 2015,100 patients with COPD in our hospital were selected,and 3 months medication guide was given to the patients.The IL-8,IL-17,NO and other oxidative stress index were observed before and after the intervention.Results Before the intervention,the IL-17,IL-8 and NO levels of patients were (77.48 ± 5.48) ng/mL,(216.48 ± 29.40) ng/mL,(59.74 ± 10.38) mol/L respectively.After the intervention,the IL-17,IL-8 and NO levels were (28.57 ± 3.94) ng/mL,(43.80 ± 3.69) ng/mL and (78.62 ± 11.49) μmol/L.The levels of IL-17 and IL-8 were significantly decreased after the intervention,the level of NO was significantly increased (t =7.49,4.83,6.59,all P ＜ 0.05).Before the intervention,the levels of FEV1,FVC and FEV1/FVC were (50.24 ± 3.27) ％,(1.24 ± 0.38) L and (53.52 ± 2.37) ％ respectively.After intervention,the levels of FEV1,FVC and FEV1/FVC were (74.46 ± 5.74) ％,(1.99 ± 0.41) L and (83.27 ± 3.25) ％ respectively.The respiratory parameters of the patients after intervention were significantly increased (t =6.38,3.89,7.02,all P ＜ 0.05).After 3 months of intervention,in 100 patients,7 cases of grade 0,33 cases of grade Ⅰ,54 cases of grade Ⅱ,6 cases of grade Ⅲ,through statistical analysis,compared with those before intervention,significantly improved (x2 =6.49,all P ＜ 0.05).Conclusion After 3 months of intervention,the patients' respiratory function limit improved,the condition improved significantly,and the medication guide for the patients with COPD has a positive effect.

ObjectiveTo explore the influence of cognitive behavioral intervention on hospitalized patients with schizophrenia.Methods100 hospitalized schizophrenia patients were randomly divided into experimental group and the control group.Routine treatment given to the patients in the control group and cognitive behavioral intervention was applied to the patients at the same time based on routine treatment in experimental group.The patients were assessed with the mini-mental state examination( MMSE),activities of daily living(ADL) and WHO battery of cognitive assessment instrument(WHO-BCAI) pre-and post-cognitive behavioral intervention.ResultsThe expenrentcl geoup and ntrol group before and after the intervention the average MMSE score was(21.8 ± 3.9),(24.4 ± 4.7 ),(21.5 ±3.4),(22.9 ±3.8) ;ADL score an average of(28.8 ±7.9),(25.1 ±4.5),(27.9 ±7.1),(25.2 ±4.8) (t =6.89,11.13,all P ＜ 0.05 ) ; cognitive function in the auditory vocabulary learning,language skills,visual identification,the connection test and cancellation tests were different ( P ＜ 0.05 or P ＜ 0.01 ).ConclusionCognitive behavioral intervention had positive influence on cognitive function in hospitalized patients with schizophrenia.

The blood brain barrier(BBB) is a barrier system between the blood and brain tissue,which can maintain relatively stable of internal environment in the central nervous system (CNS).Cerebral hypoxiaischemia and other conditions can lead to the change of the permeability of BBB.This paper reviews the latest research for the structural basis and function of BBB,and for the scope,time and mechanism of the change of BBB structure and function during hypoxic-ischemic brain injury.This theory in the regulation of BBB may play an important role in the treatment of hypoxic-ischemic brain injury and underlying diseases of CNS.

Histone deacetylase7 (HDAC7) belongs to Ⅱ a histone deacetylases.HDAC7 can alter chromosome structure and regulate gene transcription,and plays an important role in tumorigenesis and tumor angiogenesis.Increasing evidences show that HDAC7 can maintain the vascular integrity and continuity,and regulate the expression of angiogenic genes.HDAC7 also can regulate the migration and proliferation of vascular endothelial cells,and regulate angiogenic effect mediated by VEGF and other proangiogenenic factors contributing to tumor angiogenesis.Therefore,studies of the mechanisms in which HDAC7 contributes to tumor angiogenesis and the development of HDAC7 inhibitors could provide a new direction to tumor diagnosis and therapy.

Objective To study the adverse effect of omethoate on DNA in mouse sperm cells in vivo. Single cell gel electrophoresiss (SCGE) or comet assay was used to observe the damage of DNA. Methods 40 male kunming mice were randomly divided into 4 groups and were treated with omethoate by gavage at 0 mg/kg, 0.5 mg/kg, 1 mg/kg and 2 mg/kg respectively, once a day for 35 consecutive days. Results The movement and density of sperms decreased as the dose of omethoate increased, the length of comet tails in group of 1 mg/kg and group of 2 mg/kg was much longer than that in the control, the other indexes of comet assay also showed significant DNA damage. Conclusion Omethoate exposure can induce DNA damage in the sperm cells of male mice.

Objective:To study the risk factors and treatment of lymphatic fistulas after the radical operation of gastric cancer.Methods:Observed the clinical data of 631 cases after the radical operation of gastric cancer that had been received in our hospital from February 1998 to February 2008.Multiple factor analysis with logistic regression was performed for the risk factors.Results:The level of post-operative serum albumin on the first day and Ultrasonically activated shears are protective facrors against lymphatic fistulas(P

Objective:To investigate the inhibitory effect of 4'-methylether-scutellarein(4-MS),an extract from Verbena officinalis,on human choriocarcinoma JAR cell line and the possible mechanism.Methods:JAR cells were exposed to 4'-methylether-scutellarein of different concentrations for 48 h.MTT assay was used to examine the anti-proliferative effect of 4'-methylether-scutellarein.Flow cytometry was used to investigate the apoptosis and the changes of cell cycle.AO/EB double staining was applied to discriminate the apoptotic cells from dead ones.The changes of Survivin,p38-MAPK and Caspase 3 mRNA expressions were detected by RT-PCR in JAR cells treated with 4-MS.Furthermore,Western blotting assay was used to determine Survivin protein expression,phosphorylation level of p38 and Caspase 3 in JAR cells before and after 4-MS treatment.Results:4-MS inhibited the proliferation of JAR cells in a dose- and time-dependent manner(P