Objective:To establish an HPLC method for the content determination of viterxin in Stenoloma chusana( L. ) Citing. Methods:A Waters Symmetry C18 (250 mm × 4. 6 mm, 5 μm) column was used, and the mobile phase was acetonitrile-0. 1% glacial acetic acid solution (27∶73) at the flow rate of 1. 0 ml·min-1 . The detection wavelength was at 339nm, and the column temperature was 30℃. Results:The linear relationship of viterxin was good within the range from 10. 03 to 90. 27μg·ml-1 . The average recovery was 100. 48%(RSD=0. 98%). Conclusion:The established method is accurate and sensitive with good reproducibility, which can be applied in the determination of viterxin of Stenoloma chusana( L. ) Citing.

Objective To investigate the effects of long-term replacement therapy of estrogen or compound estrogen on the deposition of beta-amyloid protein (A?) in ovariectomy (OVX) rat hippocampal formation. Methods Fifty seven-month-old female Sprague-Dawley rats were randomly divided into five groups: normal control, SHAM, OVX, OVX plus 17beta-estradiol (OVX/ERT), and OVX plus compound nylestriol tablet (OVX/NL). The ovariectomy was performed in OVX, OVX/NL and OVX/ERT rats and drugs were administered orally in OVX/NL and OVX/ERT rats for 35 weeks after the ovariectomy. Then the rats in all groups were sacrificed. Immunohisto-chemistry of A?, cell counting and imaging system were used to determine the change of A? deposition levels in OVX rat hippocampal formation. Results The numbers and optical density of A?-positive neurons of all hippocampal subregions and dentate gyrus in OVX rats were markedly higher than those of normal control, SHAM,OVX/NL, and OVX/ERT rats( P

construction of a comprehensive,multifunctional and theory supported operation system is the organization basis on which occupation burnout can be scientifically and effectively interfered.Interference to occupation burnout is divided into nine subsystems by "participation" dimension which consists of organization,family and individual,and by "functional" matrix dimension which consists of the first grade prevention(development interfere),the second grade prevention(symptom interfere) and the third grade prevention(burnout interfere).Among these subsystems there exist factors of different target,content,mode and method etc.Their operation and interrelation form a complete working system via which an organization interferes occupation burnout.Promote the hospital management more scientificly and hominizaiton's in human resource.

OBJECTIVE:To investigate the status quo of the pharmaceutical care of drug consultation calls so as to facilitate the development of pharmaceutical care and promote rational drug use.METHODS:All the recordings on the pharmaceutical consultation calls from Jan.2001 to Sep.2008 were collected to analyze the compositions of consultants,problems consulted and the categories of the drugs consulted,etc.RESULTS:75.6% of the consultants were hospital employees;41.7% of the problems consulted were related to drug interactions and dosage and administration,and 22.8% of the drugs consulted were antibiotics.CONCLUSION:The drug consultation call model is convenient and feasible,and is proved to be a realistic professional pharmaceutical care.

Objective:To determine over-limit status of sodium nitrite in cooked meat products in Hunan Province,and to provide scientific evidence for making health supervision.Methods:In accordance with the national standards for sampling and testing,data were analyzed by classical and spatial statistic methods.Results:The total over-limit rate of sodium nitrite was 5.5％ in 731 samples.The relative higher sodium nitrite over-limit rates were Xiangxi Tujia and Miao autonomous prefecture and Zhangjiajie.October and February were the main months in nitrite over-limit.Five regions with over-limit of sodium nitrite in cooked meat products were detected in Hunan.Conclusion:With obvious space-time clustering,over-limit of sodium nitrite in cooked meat products is a common problem particularly in northwest Hunan and Zhuzhou City.Supervision in October should be further strengthened in above-mentioned areas to guarantee the consumers health.

Daxx is found in the nucleus where it localizes to PML oncogenic domains (PODs). Its multiple domains can interact proteins involved in transcriptional regulation and apoptotic signal transduction. In addition, Daxx is associated with viral infection、tumorigenesis and embryonic development.

Objective To evaluate the preoperative liver function and prognosis of laparoscopic cholecystectomy (LC) in patients with cirrhosis,using the Child-Turcotte-Pugh classification and the model for end-stage liver disease(MELD) score.Methods From January 2009 to June 2013,973 patients who were admitted to the Department of General Surgery of our hospital and the HuiZhou Municipal Central Hosptial were studied.Of the 373 patients with cirrhosis,38 patients were excluded because of Child C,MELD ＞ 30,or laparotomy.The remaining 335 patients who received laparoscopic cholecystectomy were randomly divided into two groups The Child grade and MELD score were retrospectively analyzed.Results There was no significant difference in intraoperative hemorrhage between the Child A group [(106 ± 11) ml] and the Child B group [(109 ± 11) ml] (P ＞ 0.05).The R ＜ 14 scores in the MELD group [(58 ± 15) ml] was significantly lower than that in the R≥ 14 group [(120 ± 28) ml] (P ＜ 0.01).There was no significant difference in postoperative complications between the Child group A (10 cases,12％) and the Child group B (17 cases,21％) (P ＞0.05).There was a significantly lower incidence in the R ＜ 14 scores in the MELD group (10 cases,12％) than the R ≥ 14 group (27 cases,33％) (P ＜ 0.05).There was also no significant difference in the hospital stay between the Child A group (9 ± 1) and the Child B group (10 ± 2)(P ＞0.05) ; the R ＜ 14 score of the MELD group (7 ± 1) was significantly less than that of the R≥ 14 group (11 ±2) (P ＜0.01).There was no significant difference in the cost of hospitalization between the Child A group (1.337 ± 0.063) and the Child B group (1.359 ± 0.089) (P ＞ 0.05) ; the R ＜ 14 group (MELD score 1.108 ± 0.123) was significantly less than that of the R ≥ 14 group (1.568-± 0.117)(P ＜ 0.01).Conclusion Compared with the Child-Turcotte-Pugh classification,the MELD score was more scientific,objective and accurate in judging the preoperative liver function.It helped to predict the amount of intraoperative hemorrhage and postoperative morbidity,reduced hospital stay and hospitalization expenses.Therefore,the MELD scoring system more objectively guided the treatment of patients with cholecystitis with cirrhosis.

OBJECTIVETo study the molecular mechanism of extracts from Euodia rutaecarpa on hepatotoxicity in mice.

METHODTotally 30 KM mice were divided into 3 groups and orally administrated extracts from E. rutaecarpa for consecutively 15 days. The expressions of Erkl/2, CDK8, CK1e, Stat3 and Src were detected by Western blotting method.

RESULTThe extracts from E. rutaecarpa could up-regulated Erkl/2, CDK8 and CK1e expressions (P <0.01) and down-regulate Stat3 and Src (P <0.01).

CONCLUSIONThe molecular mechanism of E. rutaecarpa on hepatotoxicity may be correlated with Erkl/2, CDK8, CKle, Stat3 and Src signal molecules.

Alanine Transaminase ; blood ; Animals ; Aspartate Aminotransferases ; blood ; Down-Regulation ; Drugs, Chinese Herbal ; toxicity ; Evodia ; chemistry ; Female ; Fruit ; chemistry ; Gene Expression Regulation ; drug effects ; Liver ; drug effects ; metabolism ; Male ; Mice ; Plants, Medicinal ; Signal Transduction ; drug effects ; Specific Pathogen-Free Organisms ; Triglycerides ; blood ; Up-Regulation

Objective To summarize the clinical experience of diagnosis and treatment of solid-pseudopapillary tumor of pancreas(SPTP),and to work out the diagnosis and treatment process which matches the conditions of China.Methods The clinical data of consecutive 50 patients with SPTP who had been admitted to our hospital from January 2001 to March 2007 were retrospectively analyzed.Of all patients,3 were male and 47 female.Their median age Was 24 years(13-60 years).Preoperative imaging examination revealed typical findings and tumor markers were negative.SPIP of 23 cases were located in the head of pancreas,3 in the neck of pancreas,2 in the uncinate process of pancreas.3 in the body of pancreas,18 in the tail of pancreas,and the location of SPTP of 1 case was uncertain.Forty-eight patients underwent the operation of SPTP resection,1 of exploratory laparotomy and 1 of CT-guided fine-needle aspiration biopsy of metastatic lesions.Results Forty-nine out of 50 patients were diagnosed"pancreatic mass"preoperatively.Eighteen of the 32 patients with intact tumor capsule and 11 of the 16 patients without intact tumor capsule underwent operation without pathological examination.Thirty-two patients were followed up for 3-55 months,and no metastasis or invasion of the SPTP was observed.Conclusions Yong females are in the high risk group of SPTP.CT is the most valuable preoperative imaging test.Complete surgical resection is the first choice treatment with good prognosis.Resection of the head of pancreas with the preservation of duodenum and resection of body and tail of pancreas+splenectomy are the commou modalities used for the treatment of SPTP.The intactness of the tumor capsule is an important factor in making surgical plan and deciding the necessity of intraoperative frozen section pathological examination.The most common postoperative complication of SPTP resection is pancreatic fistula.

To investigate the characteristics of acute respiratory distress syndrome(ARDS)in the casualties of a great earthquake in Wenchuan China on May 12,2008.Records of the hospitalized patients in ICU injured in the earthquakes were examined retrospectively.Among the total of 153 critical patients injured in the earthquake,52(34.0%)had ARDS.Among these 52 patients with ARDS,24(46.2%)had multiple organ dysfunction syndrome(MODS).9(17.3%)patients with ARDS dead.Approximate 34.0% of the casualties of a great earthquake in ICU had ARDS,MODS is a common associated conditions in these patients,infection phy a great role in these patients.