Objective:To investigate the effect of modified middle and upper approach for radical resection of thyroid carcinoma on neck and chest pain, cosmetic effect and postoperative quality of life in patients with early thyroid carcinoma.Methods:Ninety-seven patients with early thyroid carcinoma in the Third Hospital of Qinhuangdao City, Hebei Province from January 2017 to January 2020 were selected. According to the random digits table method, the patients were divided into the control group (traditional lateral approach for radical resection of thyroid carcinoma, 48 cases) and the observation group (modified middle and upper approach radical resection of thyroid carcinoma, 49 cases). The operation condition (operation time, intraoperative blood loss, dissection number of lymph node, drainage time, drainage volume and hospitalization time) and operative complication were compared between 2 groups; the degrees of neck and chest pain 1 d, 3 d, 1 month and 3 months after surgery were evaluated by visual analogue score (VAS); the cosmetic effect of the incision 3 months after surgery was evaluated by Vancouver scar scale; the thyroid cancer-specific quality of life (THYCA-QoL) scale was used to assess the changes in the quality of life before surgery and 3, 6 months after surgery.Results:There were no statistical differences in the dissection number of lymph node, drainage volume and hospitalization time between 2 groups ( P>0.05); the operation time, intraoperative blood loss and drainage time in observation group were significantly lower than those in control group: (116.57 ± 25.75) min vs. (129.87 ± 30.01) min, (31.25 ± 4.15) ml vs. (37.98 ± 6.34) ml and (2.57 ± 0.45) d vs. (3.32 ± 0.67) d, and there were statistical differences ( P<0.05 or <0.01). The total complication incidence in observation group was significantly lower than that in control group: 8.16% (4/49) vs. 45.83% (22/48), and there was statistical difference ( χ2 = 15.324, P<0.01). Neck VAS 1 d, 3 d and 1 month after surgery and chest VAS 3 d and 1 month after surgery in observation group were significantly lower than those in control group, and there were statistical differences ( P<0.01 or <0.05); there was no statistical difference in neck and chest VAS 3 months after surgery between 2 groups ( P>0.05). The Vancouver scar scale score 3 months after surgery in observation group was significantly lower than that in control group: (6.15 ± 1.35) scores vs. (8.43 ± 2.17) scores, and there was statistical difference ( P<0.01). The THYCA-QoL score 3 and 6 months after surgery in observation group were significantly higher than that in control group: (46.25 ± 9.87) scores vs. (40.14 ± 7.52) scores and (53.65 ± 15.14) scores vs. (46.58 ± 9.87) scores, and there was statistical difference ( P<0.01). Conclusions:Compared with the traditional lateral approach, the modified middle and upper approach radical resection of thyroid carcinoma in treatment of early thyroid carcinoma can shorten the operation time, reduce intraoperative blood loss, reduce the incidence of surgical complications and reduce early postoperative neck and chest pain. It has better cosmetic effects and improves the quality of life of the patients.

Objective To investigate the allngeneic bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs) transplantation on cardiac rehabilitation and cardiac function after acute myocardial infarction (AMI). Method Acute myocardial infarction models were made by ligating the left anterior descending coronary artery of rats. A total of 90 rats were randomly divided into the cell transplantation group and control group, 45 rars in each group. Rats in the cell transplantation group were subdivided into 3 groups according to the administration time of the allogeneic bone marrow mesenchymal stem cells (2 weeks, 4 weeks and 12 weeks), and the control group was subdivided the same as in the cell transplantation group. The success criteria included the anterior my-ocardial wall darked and the Sr-segment elevation. The rat femur bone marrow cells were separated using centrifu-gal method, mononuclear cells were isolated by lymphocyte fluid, and the third generation of mesenchymal stem cells were obtained by washing, culture, passage and marked by 4, 6 diacetyl-2-phenyl Indole (DAPI). The ani-mals' chest was opened again a week later, the stem cell suspension was injected into the border zone of infarct area, and the control group was injected with medium. Pre-operative,2 weeks, 4 weeks and 12 weeks after trans-plantation, cardiac function was tested by ultrasonic, myocardial biopsy was observed by HE conventional staining, cardiac stem cells division were observed by immunofluorescence. All data was analyzed by software SPSS 10.0, Independent-Samples T test and Chi-Square test was used, and P≤0.05 was considered as statistically signif-icant. Results The markered hone marrow mesenchymal stem cells were left in the myocardial cells and differen-tiated to eardiomyocyte-like cells. After 12 weeks, the left ventricular internal diameter of rats in cell transplanta-tion group was about (0.58±0.09) mm(P<0.05). Thickness of myocardial infarction zone was (0.25±0.01) mm (P<0.05),ejection fraction was (67.52±0.61) (P<0.05) and left ventricular fractional shortening was (39.86±3.00) (P<0.05). In the transplantation group, the DAPI marked nucleus presented as blue, and the cytoplasm of myocardial cell presented as green. HE staining showed that, in the cell transplantation group, there was less scar tissue and a large proportion of nuclear, similar to the fetal or newhom myocardium cells. Conclu-sions The homogeneus mesenchymal stem cell could repair myocardial infarction and improve cardiac function af-ter infarction.

Henoch-Schonlein purpura(HSP) is one of the most common blood capillary allergic disorder in children, and its pathogenesis has not been well explained. The studies show that the main mechanism of HSP is the abnormality of humoral immunity, and the role of IgA is emphaszed. Meanwhile, many agents such as changes in T cell function,participation of cytokine and mediators of intlammation, suscepti-bility gene and other factors also play an important role in the incidence of HSP. The pathogenesis of HSP can be better understood through extensive analysis of the immunology factors,genetic factors,and so on.

In order to investigate the effects of mouse CTLA4Ig gene-modified dendritic cells (DCs) on the survival of the corneal allografts in rats, the plasmid PG\CTLA4Ig was transfected into DCs of F344 rats mediated by Lipofectamine 2000. The expression of CTLA4Ig was detected by immunofluorescent microscopy. The effects of donor DCs on the proliferation of T cells in Lewis rats (recipients) were tested by by CCK8. Corneal transplantation was performed from F344 rats to Lewis rats. The DCs modified with CTLA4Ig gene were injected into the Lewis rats on the day 0 and 3 after transplantation. The movement of the DCs after modification in vivo was observed by immunofluorescent microscopy, and the survival of corneal allografts was evaluated by Holland criterion. The results showed that the CTLA4Ig-modified DCs could restrain the proliferation of allogenetic T cells. The CTLA4Ig-modified DCs prolonged survival of corneal allografts. (P < 0.01). It was suggested that the injection of CTLA4Ig gene-modified DCs could obviously inhabit the allograft rejection and prolong the survival of corneal allografts.

ObjectiveTo observe the clinical efficacy of bee-sting therapy in treating chronic colitis.MethodTotally 101 patients with chronic colitis were randomized into two groups, 39 cases in the control group and 62 cases in the treatment group.The treatment group was intervened by bee-sting therapy, while the control group was by medication. During the intervention, patients in the two groups were asked to keep bland diet and take more food rich in fiber instead of spicy and stimulating food. Abdominal pain, defecation pattern and frequency were observed during the study, and the clinical efficacies were compared a month later.Result The markedly effective rate was 82.3% in the treatment group versus 61.5% in the control group, and the therapeutic efficacyof the treatment group was significantly higher than that of the control group (P<0.05), and the improvements of pain relief and defecation occurred earlier in the treatment group than in the control group.ConclusionBee-sting therapy has advantages of faster pain relief and long-standing therapeutic efficacy.

Mammals have three genes with homology to the hh gene.These comprise Sonic hedgehog(Shh),Indian hedgehog(Ihh),and Desert hedgehog(Dhh).Shh has been shown to play a crucial role in embryogenesis and the development of ectoderm.Shh has the great relationship with the forming all the system.Shh-Patched-Smoothened signal pathway will lead the embryogenesis in the normal way.Otherwise,the abnormal embryogenesis,malformation,and tumor will be happened.

Polycomb group proteins Bmi-1 is the core subunits of the PRC1 complex,and also plays important roles in the regulation of neural development. This review introduces the construction of Bmi-1 and the recent study for Bmi-1 in the nervous system development and tumor generation. The important roles of Bmi-1 for sustain of the neural stem cell proliferation,regulation mechanism of neural development and tumor generation are discussed.

OBJECTIVE:To establish a method for determination of ionization equilibrium constant of sildenafil using ultraviolet spectrophotometry.METHODS:Methanol solutions of sildenafil with pH values of 2.26,8.56,9.31,9.83 and 12.38 were respectively scanned at the wavelength within the range of 200nm～400nm.The absorbability and pKa value of sildenafil were determined at the selected wavelength of 280,287,290,295 and 297nm respectively.RESULTS:The pKa value of sildenafil was 9.35?0.02.CONCLUSION:The present method is convenient and practicable.

Objective To explore the clinical , EMG and genetic characteristics of children with hereditary neuropathy with liability to pressure palsies phenotype (HNPP).Methods One case of HNPP diagnosed by gene were reported, and combined with the literature , the clinical, electromyography and genetic characteristics were summarized.Results Female patient, 11 years and 8 months, left foot could not be dorsal flexion and numbness appeared after the movement in 10 d before admission ,EMG showed multiple peripheral nerve injury .Patient's father and uncle were very similar to the history .Genetic examination showed 1363.2 kb heterozygous deletion on the chr17:14095421 -15458636, and the diagnosis was HNPP .Conclusions When limb weakness happened in children after slight stretch or compression , perform EMG examination as early as possible .Children with extensive peripheral nerve damage , limited limb paralysis , and a similar family history , should pay attention to HNPP , and gene examination should be given .

CD44 is a transmembrane receptor protein, belonging to the family of adhesion molecules, which is involved in cell-cell and cell-matrix interactions by binding with ligands such as hyaluronan. Recently a lot of researches reported that CD44 and its variant isoforms, especially CD44v6, are usually aberrantly expressed in many kinds of tumor cells, which is correlated with the invasion and metastasis of tumors and the prognosis of the patients.