AIM: To explore the surgical methods and clinical effects on young patients with congenital lower eyelid entropion.
METHODS: There were 27 patients ( 45 cases ) who suffered congenital lower eyelid entropion accepted the modified blepharosphincterectomy. The clinical effects and complications were evaluated.
RESULTS: After followed up for 6mo, 42 eyes were fully recovered, 3 eyes were unsuccessful and the cure rate was 93%, 5 eyes suffered minor lower eyelid skin folds, none had lower eyelid retraction and ectropion.CONCLUSION: Modified blepharosphincterectomy is an ideal cosmetic surgical treatment for young patients with congenital lower eyelid entropion. It is an effective surgical treatment with fewer complications.

10.3969/j.issn.2095-4344.2013.25.012

AIM: To evaluate the efficacy and complications of intravitreal injection of triamcinolone acetonide ( TA ) for the treatment of macular edema caused by pre-retinal membrane of the macular.
METHODS: Totally 23 patients ( 24 eyes ) with macular edema caused by pre-retinal membrane of the macular were treated withintravitreal injection of 4mg TA. Best-corrected visual acuity ( BCVA ) , intraocular pressure ( IOP ) , slit - lamp examination, fundus fluorescein angiography ( FFA ) and optical coherence tomography (OCT) were performed before and after treatment. The SPSS 12. 0 software was used for statistical analysis.
RESULTS: After 10, 30, 90d of treatment of TA, as compared with before treatment, visual acuity improved significantly ( P<0. 05 ), and central macular thickness (CMT) was significantly thinner (P<0. 01). The average central macular thickness decreased from 522 ± 126μm before treatment to 264±115μm, 245±128μm, 286±131μm at 10, 30, 90d after treatment. Macular edema reduced. IOP increased in 7 eyes ( 29%) , one cataract case, no other complications associated with vitreous injection.
CONCLUSION: Intravitreal injection of TA in the treatment of macular edema caused by pre - retinal membrane of the macular is simple, safe and easy to operate. It can quickly reduce macular edema, and improve the visual acuity in the short term. Part of patients may recur after injection in the first half of the year.

0.05).Conclusion: The method of constructing a highly invasive subline of bladder neoplasm is reliable,and EGFP transfection does not change the biological behavior of the cells.The obtained sublines may provide a valuable experimental platform for further study on the molecular mechanisms of bladder neoplasm metastasis.

Methylotrophy is a kind of widespread microbe which can use carbon compound as their only carbon and energy sources.It has been reported that methylotrophy can directly use one carbon com-pound to transform into their own metabolic one carbon unit,then these one metabolic one carbon units can be used as energy and carbon skeleton by organisms,which is a main part in one carbon metabolism.Because this is a novel metabolic system,it can be used in the study of biological metabolism and evo-lution.Based on the previous study about Methylobacterium sp.MB200 in our lab,here we summarized the research improvements about methylotrophy from their taxonomy,metabolism,genomics and ap-plications.

Objective To observe the effects of nebulized nitric oxide donors on pulmonary and systemic hemodynamics during pulmonary hypertension induced by hypoxia (FiO_2=0.1,for 1 hour)in newborn piglets. Methods Twenty-four anesthetized and mechanically ventilated piglets were randomly divided into four groups:(1)group S (n=6),control group;(2)group C (n=6),receiving saline after hypoxia;(3)group NTG,receiving nebulized nitroglycerin after hypoxia;(4)group SNP,receiving nebulized sodium nitroprusside after hypoxia. Mean arterial pressure (MAP)and mean pulmonary arterial pressure (MPAP)were monitored continuously. Arterial blood gas analysis was determined at baseline,1 hour after hypoxia,termination of nebulization and 0.5 h after nebulization. After nebulization,blood methemoglobin (MetHb)and plasma NO level were measured.Results Acute hypoxia resulted in a significant increase in MPAP. At 1 hour after hypoxia,MPAP in group C,group NTG and group SNP were significantly higher than that in group S(P0.05).After nebulization,there were no significant differences in plasma NO level and blood MetHb level between groups. No rebound pulmonary hypertension was observed after withdrawal of nebulization. Conclusion Both NTG and SNP nebulization could be used as selective pulmonary vasodilators for acute hypoxic pulmonary hypertension.

Objective To evaluate the influence of indoor air filtration by air purifier on the behavior of indoor radon progeny and estimate its effects on indoor radon exposure to residents.Methods The concentrations of indoor radon progeny with and without the air filtration were calculated by the theoretical model developed in this study.Based on the dose conversion factors of attached and unattached radon progeny,the mitigation effects of indoor radon exposure due to the air filtration were estimated.Verification experiments were conducted in an airtight room.Results With the air filtration rate increased,the removal efficiency of indoor radon progeny would appear more obvious.For an airtight room with its initial aerosol concentration of 10 000 cm-3,the concentration of total radon progeny would decrease by more than 90％ when the air filtration rate exceeded 10 h-1.But the concentrations of indoor unattached radon progeny would increase by using the air purifier in different extents.The variations of radon progeny concentrations and the decrease of internal doses were closely related to the filtration rate of air purifier.With the air filtration rates increased,the mitigation effects would be better and faster.The internal dose would reduce by more than 70％ when the filtration rate exceeded 5 h-1.Conclusions Although the indoor concentration of unattached radon progeny will increase by using the air purifier,it is still effective in reducing the exposure dose to residents due to the inhalation of radon progeny.

Objective To establish experimental radioactive wound model in miniature pig and observe its pathological changes in order to carry out in vivo study of radioactive wound-related diseases.Methods According to the random number table,eight adult Bama miniature pigs were divided into four groups after being anesthetized:normal control and experiment groups exposed to 0,10,20 and 30 Gy respectively using the X-ray linear accelerator.Then a puncher was use to prepare full-thickness skin defects (4 cm in length) in the irradiation site.Wound samples were collected every other week to observe wound healing.RT-PCR was used to measure expressions of tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β in wound tissues 35 days after irradiation.At the same time,Western blot was utilized to detect expressions of CD31 and collagen Ⅰ.HE staining was applied to observe histopathological changes of the radioactive wound.Results Skin wounds in normal control group healed in 35 days.Whereas in 20 and 30 Gy experiment groups,the wounds were not healed in 63 days and the healing rate was gradually weakened with the irradiation dose increasing.Expression ratio of TNF-α mRNA was 0.315 ±0.007 in normal control group,0.984 ±0.211 in 10 Gy experiment group,1.140 ±0.088 in 20 Gy experiment group,and 0.471 ±0.030 in 30 Gy experiment group.Expression ratio of TGF-β mRNA was 0.402 ± 0.018 in normal control group,1.707 ± 0.147 in 10 Gy experiment group,1.933 ± 0.078 in 20 Gy experiment group,and 0.968 ± 0.084 in 30 Gy experiment group.Expressions of TNF-α and TGF-β mRNA were significantly increased in 20 Gy experiment group compared to normal control group (P ＜ 0.05).Expressions of CD 31 and Collagen Ⅰ protein were inhibited in 20 Gy experiment group compared to normal control group (P＜0.05).Epithelialization and granulation tissue regeneration in 20 Gy experiment group were impaired.Conclusion An irradiation wound model in Bama miniature pigs can be efficiently and effectively simulated using the 20 Gy X-ray irradiation combined with full-thickness skin defect,and can be used to study the pathophysiology and therapeutic intervention of radioactive wound-related diseases in vivo.

Adolescent ; Cranial Nerve Neoplasms ; Facial Nerve ; Humans ; Male ; Neurilemmoma ; Parotid Region ; pathology

Diseases in Twins ; Epilepsy ; complications ; Epiphyses, Slipped ; etiology ; Femur Head ; Humans ; Infant ; Male