AIM: To explore the surgical methods and clinical effects on young patients with congenital lower eyelid entropion.
METHODS: There were 27 patients ( 45 cases ) who suffered congenital lower eyelid entropion accepted the modified blepharosphincterectomy. The clinical effects and complications were evaluated.
RESULTS: After followed up for 6mo, 42 eyes were fully recovered, 3 eyes were unsuccessful and the cure rate was 93%, 5 eyes suffered minor lower eyelid skin folds, none had lower eyelid retraction and ectropion.CONCLUSION: Modified blepharosphincterectomy is an ideal cosmetic surgical treatment for young patients with congenital lower eyelid entropion. It is an effective surgical treatment with fewer complications.

Objective To investigate the role of local aldosterone system in oncofetal fibronectin ( oncofetal FN) mRNA expression and reactive oxygen species (ROS) production in human mesangial cells (HMCs) exposed to short-term intermittent high glucose and the effect of Fenofibrate.Methods The HMCs were divided into 8 groups:normal glucose(NG) ;osmotic fluctuation(OF) ;mean glucose load (MGL) ;stable high glucose (SHG),short-term intermittent high glucose (IHG) ; intermittent high glucose plus eplerenone (IHGE) ; intermittent high glucose plus fenofibrate(IHGF) ; and normal glucose plus fenofibrate (NGF) groups.The mRNA expression levels of Aldosterone synthase ( CYP11 B2 ),11 β-hydroxysteroid dehydrogenase type 2 ( 11βHSD2 ) and oncofetal FN were determined by RT-PCR.The expression of CYP11B2 protein was determined by western-blot.Aldosterone level in cell culture supernatant was detected by radioimmunoassay.The expression and translocation of mineralocorticoid receptor (MR)protein were assayed with confocal laser scanning microscopy. ROS levels were determined by Fluorescence microscopy and fluorescence microplate reader.Results ( 1 ) MGL,SHG,and IHG groups showed a 2.41,3.63,and 4.45 times increase in CYP11B2 mRNA expression,and a 1.83,2.15,and 2.78 times increase in CYP11B2 protein expression,respectively,compared with NG group (P ＜ 0.05 ).The aldosterone levels of HMCs culture supernatant in MGL,SHG,and IHG groups were also increased,being 1.49,2.04,and 2.54 times of that in NG group ( P＜0.05 ),and the degree of elevation in IHG group was more marked than that in SHG group( P＜0.05 ).MR was activated and translocated from cytosol to nucleus in MGL,SHG,and IHG groups.Quantitative analysis showed the ratioes of cytosol/nucleus fluorescence intensity in MGL,SHG,and IHG groups were 15％,38％,and 53％ decreased as compared with that in NG group,and the decrease was more marked in IHG group ( P＜0.05 ).(2) Oncofetal FN mRNA expression and ROS levels in MGL,SHG,and IHG groups were increased,being 1.54,2.31,3.65 and 1.26,1.91,2.48 times of those in NG group,respectively ( P＜0.05 ),and this increase was more marked in group IHG ( P＜O.05 ).Compared with IHG group,oncofetal FN mRNA expression and ROS levels in group IHGE were significantly decreased by 54％ and 53％,and in group IHGF by 45％ and 39％. ( 3 ) CYP11B2 mRNA,protein,and aldosterone levels in IHGF group were decreased by 74％,59％,and 50％,and the activation of MR in group IHGF was inhibited when the ratio of cytosol/nuclear fluorescence intensity was increased 1.88 fold as compared with that in group IHG ( P＜0.05 ).Conclusions Increased expressions of oncofetal FN and ROS by HMCs induced by short-term intermittent high glucose were nore marked than those induced by stable high glucose.The mechanism was associated with activation of local aldosterone system.Fenofibrate may inhibit the activation of local aldosterone system and alleviate the injury to HMCs induced by intermittent high glucose.

Objective To establish experimental radioactive wound model in miniature pig and observe its pathological changes in order to carry out in vivo study of radioactive wound-related diseases.Methods According to the random number table,eight adult Bama miniature pigs were divided into four groups after being anesthetized:normal control and experiment groups exposed to 0,10,20 and 30 Gy respectively using the X-ray linear accelerator.Then a puncher was use to prepare full-thickness skin defects (4 cm in length) in the irradiation site.Wound samples were collected every other week to observe wound healing.RT-PCR was used to measure expressions of tumor necrosis factor (TNF)-α and transforming growth factor (TGF)-β in wound tissues 35 days after irradiation.At the same time,Western blot was utilized to detect expressions of CD31 and collagen Ⅰ.HE staining was applied to observe histopathological changes of the radioactive wound.Results Skin wounds in normal control group healed in 35 days.Whereas in 20 and 30 Gy experiment groups,the wounds were not healed in 63 days and the healing rate was gradually weakened with the irradiation dose increasing.Expression ratio of TNF-α mRNA was 0.315 ±0.007 in normal control group,0.984 ±0.211 in 10 Gy experiment group,1.140 ±0.088 in 20 Gy experiment group,and 0.471 ±0.030 in 30 Gy experiment group.Expression ratio of TGF-β mRNA was 0.402 ± 0.018 in normal control group,1.707 ± 0.147 in 10 Gy experiment group,1.933 ± 0.078 in 20 Gy experiment group,and 0.968 ± 0.084 in 30 Gy experiment group.Expressions of TNF-α and TGF-β mRNA were significantly increased in 20 Gy experiment group compared to normal control group (P ＜ 0.05).Expressions of CD 31 and Collagen Ⅰ protein were inhibited in 20 Gy experiment group compared to normal control group (P＜0.05).Epithelialization and granulation tissue regeneration in 20 Gy experiment group were impaired.Conclusion An irradiation wound model in Bama miniature pigs can be efficiently and effectively simulated using the 20 Gy X-ray irradiation combined with full-thickness skin defect,and can be used to study the pathophysiology and therapeutic intervention of radioactive wound-related diseases in vivo.

Objective To evaluate the influence of indoor air filtration by air purifier on the behavior of indoor radon progeny and estimate its effects on indoor radon exposure to residents.Methods The concentrations of indoor radon progeny with and without the air filtration were calculated by the theoretical model developed in this study.Based on the dose conversion factors of attached and unattached radon progeny,the mitigation effects of indoor radon exposure due to the air filtration were estimated.Verification experiments were conducted in an airtight room.Results With the air filtration rate increased,the removal efficiency of indoor radon progeny would appear more obvious.For an airtight room with its initial aerosol concentration of 10 000 cm-3,the concentration of total radon progeny would decrease by more than 90％ when the air filtration rate exceeded 10 h-1.But the concentrations of indoor unattached radon progeny would increase by using the air purifier in different extents.The variations of radon progeny concentrations and the decrease of internal doses were closely related to the filtration rate of air purifier.With the air filtration rates increased,the mitigation effects would be better and faster.The internal dose would reduce by more than 70％ when the filtration rate exceeded 5 h-1.Conclusions Although the indoor concentration of unattached radon progeny will increase by using the air purifier,it is still effective in reducing the exposure dose to residents due to the inhalation of radon progeny.

Diseases in Twins ; Epilepsy ; complications ; Epiphyses, Slipped ; etiology ; Femur Head ; Humans ; Infant ; Male

To improve the efficiency of medical research resource distribution in Beijing,based on the analysis of the existing problems we discussed the new model of resource integration.The model includes establishing all information-sharing platform and two operation modes of research project.By using this model it would be assured to achieve the best allocation efficiency and maximizing profits for Beijing medical research resources.

OBJECTIVETo establish an HPLC method for the content determination of baicalin, wogonin, chlorogenic acid, caffeic acid, cichoric acid, corynoline and adenosine in Pudilan Xiaoyan oral liquid.

METHODThe analysis was performed on a Phenomenex Luna C18 column (4.6 mm x 250 mm, 5 µm) with a gradient mobile phase of methanol-0.1% trifluoroacetic acid solution system at flow rate of 1.0 mL · min(-1). The detective wavelength was at 280 nm. The column temperature was 30 °C.

RESULTThe standard curves of seven studied components show good linearity in their concentration ranges with r ≥ 0.999 6. The average recovery was 98.73%-102.1% with RSD less than 2.6%.

CONCLUSIONThe method is rapid, simple and accurate, and can be applied for the quality control of Pudilan Xiaoyan oral liquid.

Caffeic Acids ; analysis ; Chlorogenic Acid ; analysis ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; Flavanones ; analysis ; Flavonoids ; analysis ; Succinates ; analysis

Successful pain management of perioperative shoulder arthroscopy may allow patients to go home earlier, improve the quality of life in perioperative period, and facilitate rehabilitation. A comprehensive method to perioperative pain control has three stages including preoperative, intraoperative and postoperative phase. Successful pain reduction should begin preoperatively because of an excellent communication between patient and physician, moreover, preoperative analgesia also should be administered. Intraoperative efforts should include local wound infiltration and the administration of anesthetic medication intra-articularly. Postoperative management should include oral analgesics, constant infusion devices, Patient Controlled Analgesia (PCA), sedative-hypnotic drug, continuous cryotherapy and vicarious treatment.
Acupuncture Analgesia ; Analgesia ; methods ; Analgesia, Patient-Controlled ; Arthroscopy ; Humans ; Pain, Postoperative ; therapy ; Perioperative Period ; Shoulder Joint ; surgery ; Transcutaneous Electric Nerve Stimulation

Spinal cord injury (SCI),which lead to sensory and motor function impairment,refers to traumatic injury to the spinal cord.The main pathophysiological changing process can be divided into two phases:the preliminary injury phase and the secondary injury phase.Organ dysfunction,tissue necrosis,sensory and motor function of irreversible lesions and even death can be led by SCI and the secondary injury phase.In order to prevent the secondary injury and repair the injured central nerve system,medication,surgery,biomaterials transplantation and autologous peripheral nerve transplantation have been widely studied.However,these strategies can only reach a certain level of symptoms relieve and neural restoration,which cannot reach clinical satisfaction.Therefore,SCI has been treated as a global difficult and attractive topic.Since stem cells have the potential ability to promote neurons regeneration and/or even differentiate into neural like cells,they may also have anti-inflammatory,anti-apoptotic,anti-oxidative stress,and promote secretion of trophic factors and vascularization,they brought new hope for SCI patients.Mesenchymal stem cells (MSCs) have attracted many researchers' attention for their multi characteristics including widely distribution,strong differentiation potential,easily isolating and storing and ethics avoiding.It has been proved that MSCs are effective for repairing SCI in vivo and vitro.In the latest 3 years,with the consummation of several clinical trials,a good prospect have been showed in the translation of MSCs transplantation into clinic.MSCs have been proved to have a significant prospect for clinical translation.At the same time,more specific issues were also raised.Thus,based on the latest 3 years clinical research and the author team's latest experimental results,we reviewed the MSCs on treating SCI from basic science to clinical trial and discussed its future development.

By sorting out the literature on circling acupuncture manipulation in past dynasties’ acupuncture books and famous doctors’ treatises and selecting representative doctors and works, this article summarizes, sorts out and analyzes the definition, performance, action and application of circling acupuncture manipulation and explores its essence in order to promote the inheritance of circling acupuncture manipulation as old and very effective therapy and better its clinical application.