We have studied the induction of mice spleen suppressor cell activity by Trypterygium Wilfordii Hook f. both in vivo and in vitro. We detected the suppressor cell activity in mice which were administrated with the drug for different days & found that after giving the drug for 5 or 9 days, the suppressor cell activity elevated remarkly, but for 12 & 15 days, the suppressor cell activity varied indefinitely, some were higher & some were lower. The drug inhibited the response of spleen cell to Con A too. But the release of inhibition on Con A responsibility was earlier than the recovery of elevated suppressor cell activity. Cocultured with the drug, slight promotion on cell proliferation was observed. The normal spleen cells cocultured with the drug for two days suppressed the normal spleen cell proliferation stimulated by Con A, the suppressive rate was comparable to that of Con A induced suppressor celll.

Herpes simplex keratitis (HSK) is the first inflammatory cause for uniocular blindness.The clinical inspection methods commonly used are limited to general observation,while in vivo confocal microscopy (IVCM) can provide details of cellular changes in an intravital HSK.IVCM not only gives more clinical evidence for pathology studying of HSK,but also serves as a new technology for the whole progress of HSK epidemiology.This article reviewed current researches of applying IVCM for HSK clinical observation and summarized these latest results.Morphological changes of each layer of the cornea,the specificity of these changes and clinical application of IVCM had been discussed in this article.

According to the principles of cognitive psychology,the rational-emotional therapy has effectives on patients with emotional disorder in changing activating events,transferring their belief and then correcting their emotional experience.The content of emotional therapy and psychotherapy in Inner Canon of Huangdi are:joy prevailing over melancholy,anger prevailing over anxiety,fear prevailing over joy,sorrow prevailing over anger,anxiety prevailing over fear,and so on.It has practical clinic signifi cance.

Objective:To research on the radio frequency ablation (RFA) combined with the clinical effect of chemotherapy in patients with advanced non small cell lung cancer and lung metastasis. Methods:From 2011 May to 2012 in our hospital 06 months 60 cases of metastatic non-small cell lung cancer and lung cancer patients, divided into radio frequency ablation(RFA) combined with chemotherapy group of 30 cases and a simple chemotherapy group 30 cases, referred to as the treatment group and the control group. To observe the change of tumor volume in patients with poor survival rate, years, tumor recurrence rate, and postoperative complications. Results:Three months after treatment in treatment group tumor recession(CR) in 5 cases(16.7%), part of the recession(PRa) 18(60%), partial mild recession(PRb) 6(20%), no degradation(NR), 1(3.3%), tumor size change significantly better than the control group. The treatment group patients 12 months survival rate was 86.7%, higher than that of the control group(70%). The treatment group of 12 months of tumor recurrence rate of 6.7%, lower than that of the control group(26.7%). No obvious adverse complications of the treatment group of 30 patients after operation. Conclusion:Radiofrequency hyperthermia ablation combined with chemotherapy in treatment of advanced non small cell lung cancer and metastatic lung cancer clinical effect is obvious.

BACKGROUND:Insulin-like growth factor-1 can promote mitosis of fibroblasts as wel as periodontal cellgrowth, differentiation and synthesis of extracelular matrix.
OBJECTIVE: To observe the effects of chitosan/colagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cels.
METHODS: The human periodontal ligament cels were seeded on chitosan/colagen composite scaffold carrying insulin-like growth factor-1 and ordinary colagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; celladhesion and proliferation were detected at days 1, 7 and 28.
RESULTS AND CONCLUSION:The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary colagen scaffold at 1, 24 hours and 1 week after cellseeding (P < 0.01). The celladhesion and proliferation showed no difference between two groups at day 1 after cellseeding, but became significantly higher in the composite scaffold than that in the ordinary colagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/colagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cels.

BACKGROUND:Adiponectin and periodontal disease are closely related with type 2 diabetes. OBJECTIVE:To detect the expression of adiponectin in periodontal tissue of Kunming mice of diabetes periodontitis, and explore the effects on periodontal tissue pathology and prognosis. METHODS:Sixty 3-week-old Kunming mice were divided into three groups according to the different modeling methods:control group (normal feeding), diabetes group (diabetic model was established by injection of al oxan), diabetes and periodontitis group (periodontitis model was established by local ligation and bacteria developed). Each group contained 20 rats. RESULTS AND CONCLUSION:At 20 days after modeling, the blood glucose, fasting insulin and insulin resistance index of mice in the diabetes group were higher than that in the control group (P<0.05). ELISA, RT-PCR and western blot analysis results showed that, adiponectin gene and protein expression levels in periodontal tissues were the highest in the control group, then in the diabetes group, and the lowest in the diabetes and periodontitis group, at 1, 3, 6, months postoperatively, respectively (P<0.05). The periodontal gum tissue inflammation and histopathological changes were observed at 1, 3, 6, months postoperatively. The results showed that, the inflammation in periodontal tissues was the most obvious in the diabetes and periodontitis group, and was visible in the diabetes group, but the inflammation was not present in the control group. Experimental findings indicate that, the down-regulation of adiponectin expression in the periodontal tissue is one of the important factors that trigger and aggravate the onset of periodontitis.

Nitric oxide (NO) is the most important known endogenous vasodilator factor. The competitive inhibitor asymmetric dimethylarginine (ADMA) of endothelial nitric oxide synthase (NOS) inhibits NO synthesis, which makes NO/NOS pathway to be obstructed and the reduction of NO synthesis. The studies in recent years have suggested that ADMA is as-sociated with the occurrence and development of diabetes with cerebrovascular disease. It has provided new insights into further clarifying the mechanism of the disease.

Objective:To systematically assess the value of 2 dimension color Doppler ultrasound(2D-CDUS) in diagnosis,surgical treatment and interventional therapy of carotid artery stenosis. Methods: Twenty seven carotid stenosis patients were examined before and after operation with 2D-CDUS. The morphology and hemodynamic of carotid artery were detected. Results: Pre-operative examination showed that there were 17 cases with stenosis degree being 50%-70%, 2 cases 71%-79%, 5 cases 80%-90%, 3 cases 90%-99%. Twelve cases were operated on with carotid endarterectomy(CEA), 15 cases with percutaneous transluminal angioplasty and stent(PTAS). After operation all the vessels with CEA were patent, colored well and blood velocity was close to normal. Residual intima was observed floating in the blood vessel in only one patient. In the patients treated by PTAS, the location and courser of the stents were normal,and the vessels were patent. Conclusion: 2D-CDUS is convenient to use and non-invasive, which can assess extracranial carotid stenosis accurately before operation, assess the curative effect and detect the complications after operation.

Objective:To assess the value of Transcranial Two Dimensional Color Doppler Ultrasound(TC-2D-CDUS) in diagnosis of carotid artery stenosis and in follow-up study after surgical treatment.Methods: Thirteen patients with carotid artery stenosis, who were excluded from vertebral or intracranial diseases, were detected before and after operation with TC-2D-CDUS. Results: TC-2D-CDUS indicated that 2 patients' image were not satisfactory, and the blood velocity of middle cerebral artery (MCA) in the same side of lesion was lowered to different extents in the other 11 patients. The waveform was blunted in 3 patients with their systolic peak disappeared, acceleration time prolonged, and the direction of the anterior cerebral artery(ACA) in the same side inverted.After operation,TC-2D-CDUS indicated that MCA blood velocity and the direction of ACA were normal. The blood distribution was symmetric in bilateral brains. Conclusion: TC-2D-CDUS can provide intracranial hemodynamic information. It can be used in the follow-up study after operation,helping to make accurate judgement and discover complications.

Objective To evaluate the clinical efficacy of cefotaxime combined with gamma globulin on neonatal septicemia. Methods The subjects of this study were selected from 88 cases of neonatal septicemia admitted in Jiangyou No.903 hospital from April 2014 to April 2017. They were randomly divided into two groups, each with 44 cases. The control group was given symptomatic treatment and cefotaxime, while the observation group symptomatic treatment, cefotaxime and gamma globulin as well. After 7days of treatment, the overall effective rates, case fatality rates, clinical symptoms (body temperature, resistance to milk, neurological symptoms), time for improvement and hospital stay were compared between the two groups. Results The overall effective rate of the observation group was 95.45％, much higher than 72.73％ of the control group (P<0.01). The observation group had much lower case fatality rate than the control group did (2.27％ vs 18.19％) (P<0.05). The time the observation group took for improvement in the condition of body temperature, resistance to milk, neurological symptoms, and hospital stay was (3.25±1.07) days, (4.93±1.96) days, (5.92±1.58) days, and (6.80±1.94) days respectively, all significantly shorter than the time the control group took (P<0.01). Conclusion The combination of cefotaxime and gamma globulin is effective in the treatment of neonatal septicemia. It can significantly reduce case fatality rate and shorten the time for clinical symptoms and hospital stay.