Herpes simplex keratitis (HSK) is the first inflammatory cause for uniocular blindness.The clinical inspection methods commonly used are limited to general observation,while in vivo confocal microscopy (IVCM) can provide details of cellular changes in an intravital HSK.IVCM not only gives more clinical evidence for pathology studying of HSK,but also serves as a new technology for the whole progress of HSK epidemiology.This article reviewed current researches of applying IVCM for HSK clinical observation and summarized these latest results.Morphological changes of each layer of the cornea,the specificity of these changes and clinical application of IVCM had been discussed in this article.

We have studied the induction of mice spleen suppressor cell activity by Trypterygium Wilfordii Hook f. both in vivo and in vitro. We detected the suppressor cell activity in mice which were administrated with the drug for different days & found that after giving the drug for 5 or 9 days, the suppressor cell activity elevated remarkly, but for 12 & 15 days, the suppressor cell activity varied indefinitely, some were higher & some were lower. The drug inhibited the response of spleen cell to Con A too. But the release of inhibition on Con A responsibility was earlier than the recovery of elevated suppressor cell activity. Cocultured with the drug, slight promotion on cell proliferation was observed. The normal spleen cells cocultured with the drug for two days suppressed the normal spleen cell proliferation stimulated by Con A, the suppressive rate was comparable to that of Con A induced suppressor celll.

According to the principles of cognitive psychology,the rational-emotional therapy has effectives on patients with emotional disorder in changing activating events,transferring their belief and then correcting their emotional experience.The content of emotional therapy and psychotherapy in Inner Canon of Huangdi are:joy prevailing over melancholy,anger prevailing over anxiety,fear prevailing over joy,sorrow prevailing over anger,anxiety prevailing over fear,and so on.It has practical clinic signifi cance.

Objective To evaluate the application efficacy of responsibility system for holistic nursing system in high-quality nursing service in the department of respiratory medicine.Methods Department of respiratory medicine unit which was the quality nursing service demonstrated unit in our hospital was selected.The nursing responsibility-bound grouping system was first performed and the nurse-incharge would provide the patients with whole and continuous nursing service.Before and 6 months after the application,the nursing job satisfaction was conducted and the nursing grouping system satisfaction was also evaluated among nurses and doctors.Results Six months after the application of nursing responsibility-bound grouping system,the patient satisfaction with nursing job was raised obviously in the unit.Conclusions The application of responsibility-bound grouping system can not only promote the activity but also mobilize the enthusiasm of nurses,and then high-quality nursing service was resulted.So the service system was worthy of clinical application.

BACKGROUND:Insulin-like growth factor-1 can promote mitosis of fibroblasts as wel as periodontal cellgrowth, differentiation and synthesis of extracelular matrix.
OBJECTIVE: To observe the effects of chitosan/colagen composite scaffold carrying insulin-like growth factor-1 on proliferation of human periodontal ligament cels.
METHODS: The human periodontal ligament cels were seeded on chitosan/colagen composite scaffold carrying insulin-like growth factor-1 and ordinary colagen scaffold. The release of recombinant human transforming growth factor-β1 was detected at 1, 24 hours and 1 week after culture; celladhesion and proliferation were detected at days 1, 7 and 28.
RESULTS AND CONCLUSION:The release rate of recombinant human transforming growth factor-β1 in the composite scaffold was significantly lower than that in the ordinary colagen scaffold at 1, 24 hours and 1 week after cellseeding (P < 0.01). The celladhesion and proliferation showed no difference between two groups at day 1 after cellseeding, but became significantly higher in the composite scaffold than that in the ordinary colagen scaffold at days 7 and 28 (P < 0.01). These findings indicate that chitosan/colagen composite scaffold carrying insulin-like growth factor-1 can significantly promote the proliferation of the human periodontal ligament cels.

Objective:To systematically assess the value of 2 dimension color Doppler ultrasound(2D-CDUS) in diagnosis,surgical treatment and interventional therapy of carotid artery stenosis. Methods: Twenty seven carotid stenosis patients were examined before and after operation with 2D-CDUS. The morphology and hemodynamic of carotid artery were detected. Results: Pre-operative examination showed that there were 17 cases with stenosis degree being 50%-70%, 2 cases 71%-79%, 5 cases 80%-90%, 3 cases 90%-99%. Twelve cases were operated on with carotid endarterectomy(CEA), 15 cases with percutaneous transluminal angioplasty and stent(PTAS). After operation all the vessels with CEA were patent, colored well and blood velocity was close to normal. Residual intima was observed floating in the blood vessel in only one patient. In the patients treated by PTAS, the location and courser of the stents were normal,and the vessels were patent. Conclusion: 2D-CDUS is convenient to use and non-invasive, which can assess extracranial carotid stenosis accurately before operation, assess the curative effect and detect the complications after operation.

OBJECTIVE:To study the method of preparing PEG-SOD enteric-coated capsuls and quality standard.ME_ THODS:Orthogonal design was used to select the optimal conditions for preparing PEG-SOD enteric-coated capsuls and the relevant quality standard of PEG-SOD enteric-coated capsuls was established according to the China Pharmacopoeia(2000). RESULTS:The optimal conditions were as follows:the content of PEG-SOD per capsule was40000IU;the weight ratio of stabilizer lactose was2.5%;filler was carboxymethyl starch;drying process was at60℃for20min.The preparation was ac?corded with the relevant quality standard.CONCLUSION:The technic of preparation is feasible,the method of quality control is reliable and the quality of preparation is stable.

Objective:To research on the radio frequency ablation (RFA) combined with the clinical effect of chemotherapy in patients with advanced non small cell lung cancer and lung metastasis. Methods:From 2011 May to 2012 in our hospital 06 months 60 cases of metastatic non-small cell lung cancer and lung cancer patients, divided into radio frequency ablation(RFA) combined with chemotherapy group of 30 cases and a simple chemotherapy group 30 cases, referred to as the treatment group and the control group. To observe the change of tumor volume in patients with poor survival rate, years, tumor recurrence rate, and postoperative complications. Results:Three months after treatment in treatment group tumor recession(CR) in 5 cases(16.7%), part of the recession(PRa) 18(60%), partial mild recession(PRb) 6(20%), no degradation(NR), 1(3.3%), tumor size change significantly better than the control group. The treatment group patients 12 months survival rate was 86.7%, higher than that of the control group(70%). The treatment group of 12 months of tumor recurrence rate of 6.7%, lower than that of the control group(26.7%). No obvious adverse complications of the treatment group of 30 patients after operation. Conclusion:Radiofrequency hyperthermia ablation combined with chemotherapy in treatment of advanced non small cell lung cancer and metastatic lung cancer clinical effect is obvious.

BACKGROUND:Adiponectin and periodontal disease are closely related with type 2 diabetes. OBJECTIVE:To detect the expression of adiponectin in periodontal tissue of Kunming mice of diabetes periodontitis, and explore the effects on periodontal tissue pathology and prognosis. METHODS:Sixty 3-week-old Kunming mice were divided into three groups according to the different modeling methods:control group (normal feeding), diabetes group (diabetic model was established by injection of al oxan), diabetes and periodontitis group (periodontitis model was established by local ligation and bacteria developed). Each group contained 20 rats. RESULTS AND CONCLUSION:At 20 days after modeling, the blood glucose, fasting insulin and insulin resistance index of mice in the diabetes group were higher than that in the control group (P<0.05). ELISA, RT-PCR and western blot analysis results showed that, adiponectin gene and protein expression levels in periodontal tissues were the highest in the control group, then in the diabetes group, and the lowest in the diabetes and periodontitis group, at 1, 3, 6, months postoperatively, respectively (P<0.05). The periodontal gum tissue inflammation and histopathological changes were observed at 1, 3, 6, months postoperatively. The results showed that, the inflammation in periodontal tissues was the most obvious in the diabetes and periodontitis group, and was visible in the diabetes group, but the inflammation was not present in the control group. Experimental findings indicate that, the down-regulation of adiponectin expression in the periodontal tissue is one of the important factors that trigger and aggravate the onset of periodontitis.

Nitric oxide (NO) is the most important known endogenous vasodilator factor. The competitive inhibitor asymmetric dimethylarginine (ADMA) of endothelial nitric oxide synthase (NOS) inhibits NO synthesis, which makes NO/NOS pathway to be obstructed and the reduction of NO synthesis. The studies in recent years have suggested that ADMA is as-sociated with the occurrence and development of diabetes with cerebrovascular disease. It has provided new insights into further clarifying the mechanism of the disease.