BACKGROUND:The creep performance of bone tissues during exercise is achieved through the microstrain,and the holosteous torsion can completely retiect the biomechanicel charactedstics of bone tissues.OBJECTIVE:To determine the creep characteristics of rabbit tibia under the torsion load following exercise.DESIGN,TIME AND SETTING:A parallel controlled animal observational experiment was performed at the Laboratory of Biomechanics in Orthopedic Hospital of Sichuan Province between May 2005 and July 2007.MATERIALS:A total of 32 Japanese white rabbits,irrespective of genders,aged 6 months,were divided into exercise group(n=24)and control group(n=8).METHODS:Exercise group animals were placed in cage to receive electrical stimulus.0.1-0.2seconds once every 20 seconds.Following the stimulation,the animals ran and jumped for several paces along with the cage and made a tum,15 minutes once,three times per day,totally 45 minutes,at the interval of 15 minutes.The exercise was carried out five days per week for 4successive weeks.The control group was fed as exercise group.but received no exercise.MAIN OUTCOME MEASURES:After 4 week exercise.the tibias were griped by the torsion clamping apparatus,the creep characterizes of anterior,posterior,medial and lateral sides of tibia under 0.52 N·m torsion load within 500 seconds were determined by strain gauge technique.RESULTS:Under torsion load,the creeps were present obviously in the rabbit tibia,reached the creep curve inflection point at 2seconds,and then gradually appeared.After 4 weeks,the creeps of tibias at anterior and posterior side in the exercise group were less than control group;in the control group,the anterior side of tibia was remarkably greater than the medial side.After exercise.the creep was the smallest in anterior side,which was less than the lateral side(P＜0.05).CONCLUSlON:The creep characteristics of tibia are anisotropic,the deformation at the anterior and posterior sides is greater than that at the medial and lateral sides.Exercisecan alter the creep charactedstice of rabbit tibia and reduces the creep at the anterior and posterior sides.

The delta-shaped anastomosis is an emerging operation method in digestive tract reconstruction following total laparoscopic distal gastrectomy (TLDG).With the advantages of small surgical trauma,short anastomosis time and simple manipulation,it has been gradually accepted by surgeons.However,restricted by small manipulation range,restrictive visual range and large anastomotic lumen,there are controversies in complete resection of tumor,intraoperative indexes,postoperative recovery and prevention and treatment of complications about TLDG with delta-shaped anastomosis.In this paper,the operative indications,intraoperative indexes and postoperative recovery of TLDG with delta-shaped anastomosis were reviewed.

Objective To facilitate a better understanding of the progress in the research, diagnosis and treatment of Peutz Jeghers syndrome (PJS).Methods Almost all the papers related to PJS from various magazines published in English and Chinese in recent years were reviewed. Current progresses in PJS research and related diagnosis and treatment were discussed in this review.Results PJS is a rare inherited disease with autosomal dominant trait, which is characterized by the presence of hamartomatous gastrointestinal polyps and mucocutaneous pigmentation of the lips, buccal mucosa, and digits. This syndrome is commonly complicated with intestinal obstruction, bleeding, or intussusception,and patients with this disease are at high risk for the development of both GI and extraintestinal malignancies. STK11 on chromosome 19p13.3 are responsible for most cases of PJS. The polyps of PJS tend to have a high incidence of malignant change, and the recurrence of malignancy after treatment is also high. Conclusion The STK11 has been identified as one of the main genes responsible for PJS and has close correlation with formation and development of tumors. Patients with PJS are at high risk for the development of both GI and extraintestinal malignancies.

(Ver)-induced human retinal pigment epithelium (RPE) cells apoptosis.hours to induce RPE cells apoptosis.The expression of apoptotic effector gene caspase-3 was assessed by reverse transcription polymerase chain reaction (RT-PCR).Single cell was measured using fluorescence indicator Fura-3/AM with MetaFluo4.5/coolsnapfx/IX70 intracellular Ca2+ fluorescence imaging system.RPE cells and it significantly increased after co-cultured with Ver.The fluorescence in resting RPE cells was strong and distributed throughout the cells.The nucleus appeared more fluorescent than the cytoplasm.Calcium fluorescence of RPE cells attenuated after co-cultured with Ver.[Ca2+]i homeostasis might play pivotal roles in Ver-induced RPE cells apoptosis.

Objective: To study the expressions of MMP-2 and TIMP-2 in pancreatic carcinoma and their relationship with tumor invasion, local metastasis and prognosis of the carcinoma. Methods: The expressions of MMP-2 and TIMP-2 were examined in 32 patients with pancreatic carcinomas by S-P immunohistochemical technique and the correlation with pathological tumor parameters were analyzed. Survival analysis was made by using the Kaplan-Meier method. Results: The positive rates of MMP-2, TIMP-2 in 32 patients with pancreatic carcinoma were 56. 25% and 75. 00%, which were significantly higher than those of the controls(P<0. 05). Expressions of MMP-2 and TIMP-2 were independent of sex, age, histological grading and type, but well correlated with the lymph node metastasis and TNM clinical staging (I and III, II and III). There was a significant association between MMP-2, TIMP-2 and prognosis in pancreatic carcinoma. Conclusion: MMP-2 and TIMP-2 might be useful markers for biological aggressiveness of this malignancy and might contribute to the invasive properties of pancreatic carcinoma, which can be used to evaluate the prognosis of patients.

Epicanthoplasty was performed in 98 patients with the epicanthal fold. Epicanthal fold was classified into three categories-minimal degree, moderate degree, severe degree-according to its severity. In accurate anatomic dissection in medial canthal area, the pathologic mechanism of the formation of the epicanthal fold, I think, are not only the redundancy of skin, but also the early downward insertion of the preseptal and pretarsal portion of orbicularis oculi muscle. A new surgical technique is described for the correction of the epicanthal fold. The procedure is simple to perform and uniformly gives good results.
Humans ; Skin

Objective IL-2,6 production by PBMNC (peripheral blood mononucleat cells) were ineaeured with an attempt to illustrate the immune function of children with bronchopneumonia.Methods The biological activities of IL-2,6 were measured by CTLL and CESS cell lines respectively In addition, mitogen-stimulated blastogenic transformation (MSBT) was also measured. The results were analysed statistically. Patients or Other Farticipants T enty-eight cases of children with bronshopneumonia, male, 16,female: 12. Age: 8 months to 7 years, average 3. 9 years. 15 normol controls, male: 8, female 7.Age: 2 to 6 years, average 3 .5 years.Interventions 5～6ml of venous blood was obtaincd, followed PBMNC were centrifugalized by. FicollHypaque. Wash three:times with RPMI-1640 medium. Resuspend cell at 5 ?106/ml. Add 1% PHA and culture for 48h. Horvest supernatant and store at - 20℃.Measurement and Main Rcsults The levels of IL-2 in children with bronchopneumonia and normal children were (CPM) 9 538 + 3 363, 16 152 ? 3 411 (t test, P

Objective To observe the changes of systolic time interval after neonatal asphyxia and explore its relationship with clinical manifestation and prognosis. Methods Tow-dimensional and Doppler echocardiography were employed to detect tow - dimension parameters and left and right ventricular STI in 27 mild and 18 severe asphyxiated neonates as well as 14 normal controls and its relationship with clinical manifestation and prognosis was analyzed. Results There was no difference in cavity and thickness of heart and great arteries between normal and asphyxiated neonates. In acute stage mild and severe asphyxiated neonates had a shorter right ventricular ejection time (RVET) than normal neonates and a longer right ventricular prejection period (RPEP) was found in severe neonates than that in normal and mild asphyxiated neonates. Left ventricular preejection time (LPEP) was prolonged in comparison with normal neonates in acute stage and convalescence. There were more cases with increased RPEP/RVET in severe asphyxiaed group than those in mild and normal group in acute stage. The incidence of heart failure in acute stage and disability in late period was higher in cases with increased RPEP/RVET than that with normal RPEP/RVET. Conclusion Asphyxia has more severe damage to right ventricle than that to left ventricle. Cases with increased RPEP/RVET are prone to suffering from heart failure in acute stage and more likely to undergo disability in late period.

In recent years the loss of humanity spirit in medicine has been the undeniable fact,which accordingly pro-vokes criticisms from patients and media.To reshape the perfect image of medical circle,it is pressing to transcend theutilitarianism of medical education , enhance the humanity education and develope the spirit of humanity.

Objective To study the expressions of MMP-2 and TIMP-2 in pancreatic carcinoma and their relationship with tumor invasion, local metastasis and prognosis of the carcinoma. Methods The expressions of MMP-2 and TIMP-2 were examined in 32 patients with pancreatic carcinomas by S-P immunohistochemical technique and the correlation with pathological tumor parameters were analyzed. Survival analysis was made by using the Kaplan-Meier method. Results The positive rates of MMP-2, TIMP-2 in 32 patients with pancreatic carcinoma were 56.25% and 75.00%, which were significantly higher than those of the controls(P＜0.05). Expressions of MMP-2 and TIMP-2 were independent of sex, age, histological grading and type, but well correlated with the lymph node metastasis and TNM clinical staging(Ⅰ and Ⅲ, Ⅱ and Ⅲ). There was a significant association between MMP-2, TIMP-2 and prognosis in pancreatic carcinoma. Conclusion MMP-2 and TIMP-2 might be useful markers for biological aggressiveness of this malignancy and might contribute to the invasive properties of pancreatic carcinoma, which can be used to evaluate the prognosis of patients.