0.05),an effective mA seconds in the experimental group(296.74?19.15)mAs,than in the control group 350 mAs was reduced about 15.4%(P

Arm lymphedema is a common and persistent complication of breast cancer surgery. Surgery or radiotherapy damages axillary lymphatic vessels and obstructs lymphatic reflux, leading to the accumulation of lymph fluid in the superficial soft tissues of the upper limbs and the subsequent formation of edema. Clinical treatment of breast cancer-related lymphedema remains a worldwide problem. Currently, there is still no cure.

BACKGROUND:Decidua tissue is one of the most important sources of endometrial stem cel s, from which stromal stem cel s can differentiate into various kinds of cel s under contain inductions. OBJECTIVE:To observe the differentiation of endometrial stromal stem cel s isolated from early-pregnancy decidua into endometrial epithelial cel s. METHODS:Endometrial stromal stem cel s from early-pregnancy decidua were cultured, identified and directional y induced. Then induced cel s were randomized into test group cultured in conditioned medium and control group cultured in normal culture medium containing fetal bovine serum. Expression of keratin was detected by immunofluorescence assay and western bolt assay in the two groups. RESULTS AND CONCLUSION:The endometrial stromal stem cel s from early-pregnancy decidua were successful y isolated and cultured, and presented with a S-shaped curve. Within 1-48 hours after inoculation, cultured cel s were in the adaptation phase;then, the cel s proliferated rapidly and began to grow logarithmical y at 48 hours after culture;after 60 hours of culture, the cel growth rate decreased, with a steady rise in the cel curve. Results from the flow cytometry detection showed that the endometrial stromal stem cel s could express CD73 and CD90, but not express CD34, CD45 and cytokeratin. Immunofluorescence findings showed the expression of keratin negative in the control group, and mostly positive in the test group. Besides, the relative expression of keratin in the test group was significantly higher than that in the control group (P<0.05). These results suggest that the endometrial stromal stem cel s can be isolated from the human decidua in early pregnancy, and differentiate into endometrial epithelial cel s by directional induction.

As a new learning method,mind map presents learning materials in a visual,radical and non-linear way.Application of mind map in teaching of pathophysiology could help teachers get the thread of textbook,enhance students'motivation for learning,help students get the main points and improve their efficiency.Mind map could make students think in a way of problem-based learning,and its advantage in training up divergent thinking could also develop students'innovation ability.

OBJECTIVE: To observe the curative effect of Rifampicin Injection-Capsule sequential therapy for retreatment of smear positive pulmonary tuberculosis(TB) at intensive stage.METHODS: 80 patients with smear positive pulmonary tuberculosis at intensive stage were enrolled: 40(treatment group) were randomly assigned to receive HZES plus Rifampicin Injection-Capsule sequential therapy,and another 40(control group) to receive HZES plus Rifampicin capsule.Follow-up of sputum negative conversion rate,pulmonary lesion,and toxcity were scheduled at the end of first and second month during intensified stage.RESULTS: At the end of first and second month during intensified stage,the sputum negative conversion rates were 82.5% and 97.5% respectively in the treatment group versus 65.0% and 85.0% respectively in control gorup;the X-ray imaging test of chests revealed that the responsive rates were 17.5% and 40.0% in the treatment groups versus 10.0%and 25.0% in the control group.At the end of second month,the cavity closure(or diminution) rate was 41.7% in the treatment group versus 22.7% in the control group;the incidence of hepatic lesion was 22.5% verus 25.0% and that of gastrointestinal tract toxcity was 20.0% versus 45.0%.CONCLUSION: Rifampicin Injection-Capsule sequential therapy was proved to be of rapid onset of effect,remarkable efficacy,less toxicity and satisfactory efficacy in controlling symptom,shortening bacterial clearance time,reducing side effects and treating severe TB.

Objective To investigate the variation of coagulation parameters in type 2 diabetes mellitus(T2DM)patients with microvascular complication. Methods Coagulation and fibrinolysis parameters were measured in 40 healthy controls (control group) and 80 T2DM patients (T2DM group) with (47 patients) and without (33 patients) microvasular complications. Results Compared with those in control group, the serum levels of fibrinogenand D-D dimmersin in T2DM group were found to be increased significantly:(4.29±1.70) mg/L vs. (3.12±0.49) mg/L, 0.395 (0.265-0.910) mg/L vs. 0.215 (0.163-0.300) mg/L, P<0.05;while the activity of antithrombin III and protein S levels were significantly decreased in T2DM group:(94.11±25.04)%vs. (103.90±12.48)%,(70.23±23.22)%vs. (90.35±17.35)%, P<0.05. Amongst the T2DM patients, the serum levels of APTT, fibrinogen,D-D dimmers, and the activity of protein S in patients with microvascular complication were found to be significantly higher than those in patients without microvascular complication:(38.09±5.73) s vs. (34.53±4.13) s,(4.60±1.93) mg/L vs.(3.86±1.21) mg/L, 0.630(0.320-1.200) mg/L vs 0.310(0.240-0.405) mg/L, (79.4± 22.16)%vs. (57.15±18.05)%, P<0.05. Conclusion Hypercoagulable state and decreased anti-coagulation ability may contribute to the risk of development of microvascular complication in T2DM patients.

Objective To investigate the perioperative health care to patients with perineal endometriosis (PME) involving anal sphincter.Method The clinical data of 17 patients diagnosed with PEM involving anal sphincter at the Peking Union Medical College Hospital from January 2009 to June 2013 were analyzed retrospectively.Results The perineal incisions in all cases were satisfactorily healed,without any fatal incontinence or other complications.There was only one case of recurrence,accounting for 5.9%.Conclusion Preoperative psychological nursing,postoperative close observation on the incisions,diet instructions,management of defecation and targeted health education can all contribute to patients’ recovery.

Objective:To evaluate the clinical outcome of transcrestal maxillary sinus floor elevation(TMSFE)in the presence of pseudocyst.Methods:14 cases with pseudocyst were treated by TMSFE.In the operation following osteotome,pseudocyst was not ex-cised and sinus floor membrane was elevated,15 implants were placed for the 14 cases.Definite prosthesis was delivered about 6 months after implant placement.CBCT examination was done before,immediately after and 1-year after surgery,the cyst condition, the gain of vertical bone height and implant stability over time were observed.Results:Pre-operation residual ridge height of the alveo-lar crest was (6.85 ±1.07)mm.The sinus membrane was successfully elevated in all sites without perforation (mean elevation height,6.93 ±2.07)mm.All 15 implants were ossteointegrated.At 1-year revisit the gained bone height was (12.76 ±2.03)mm. Cyst grew bigger in 3 cases,not changed in 3 cases,became smaller in 5 cases and disappeared in 3 cases.Conclusion:Pseudocyst excision is not necessary in TMSFE.

OBJECTIVE:To study the competitive condition and development strategy of China's pharmaceutical industry.METHODS:Applying the methods of industry economy theory.RESULTS & CONCLUSION:Features of pharmaceutical industry competition find expression in different products competition,relative monopolistic competition and scale dominant competition.China's pharmaceutical industry is developing rapidly.But its degree of centralization,profit,scale effect and ability of research and development is low,it is still in disperse and low level competitive condition.In the course of establishing new management system,we need setting up effective mechanism of industry policy,carrying out different competitive strategy,supporting good enterprises and pushing formward intensive development of China's pharmaceutical industry.

Acupuncture Therapy ; Adult ; Colitis, Ulcerative ; therapy ; Female ; Humans