No abstract available.
Blood Group Antigens* ; Humans

No abstract available.
Blood Group Antigens* ; Humans

No abstract available.
Blood Group Antigens*

OBJECTIVE: To explore the molecular biology of D variant blood group with RHD*95A genotype and the genetic mechanism of its generation. METHODS: A total of 6 samples from 3 generations of a family were analyzed. RHD blood group was identified by saline test tube and microcolumn gel card method. 10 exons of RHD gene were amplified by Polymerase Chain Reaction-Sequence Specific Primer (PCR-SSP) and analyzed by direct sequencing. Homology modeling was used to compare the structural differences between mutant RHD protein and wild-type RHD protein. RESULTS: The proband was identified as D variant by serological identification, RHD gene sequencing directly detected a c. 95 c > A mutation in exon 1 that leads to encoding the 32-bit amino acids by threonine Thr (T) into aspartic acid Asn (N), the rest of the exon sequences were normal compared with the normal RHD*01 gene. In the family, the proband's father, grandmather and uncle were all carried the same RHD*95A allele. Protein modeling results suggested that the hydrogen chain connected to the 32nd amino acid residue was changed after p.T32N mutation, which affected the structural stability of RHD protein. CONCLUSION The first genetic lineage of the RHD*95A gene was identified in a Chinese population. The c.95C>A mutation in RHD gene was found in the family, which resulted in reduced expression of RHD antigen and showed D variant, the mutation could be stably inheritable. Gene identification and protein structure analysis of D variant population is helpful to explore the molecular mechanism of its formation and ensure the safety of blood transfusion.
Humans ; Blood Group Antigens

Study on 12 transplanted couples at Hue Central Hospital from 2001 to now. Transplanted selections were based on the compatibility of blood groups and HLA (more than 3/8). Anti-interleukin 2 was used in cases of HLA compatibility of 3/6 to help expanding the selections of donor-receiver. Transplanted patient who had positive HbsAg but no symptoms, normal level of serum liver enzymes, can be transplanted and carefully followed up post-operative serum liver enzymes. Gradually decrease doses of corticoid therapy could help to limit side-effects of corticoid.
Kidney Transplantation ; Blood Group Antigens ; Blood ; Epidemiology

As a stable genetic marker of human, blood group is expressed in a polymorphic system in the population. Blood group and pathogens mainly produce effects through the interaction between antigens and antibodies. On the one hand, they can promote pathogen colonization, invasion or evasion of host clearance mechanism, and on the other hand, they can make some hosts less susceptible to corresponding pathogens. By exploring the molecular mechanism between the blood group system and pathogenic microorganisms, it can provide a scientific basis for the treatment of human related diseases and the development of vaccines.
Blood Group Antigens/genetics* ; Disease Susceptibility ; Humans

Currently, the cell surface antigen A,B,O(H) is thought to be an important indicator of malignant potential in bladder carcinoma. Herein, we performed SRCA test in 54 bladder carcinoma for detection of such an isoantigen, comparing the SRCA result to its tumor grade and stage. Also, various significances including the clinical application of SRCA test for the management of the bladder carcinoma were discussed. The results were as follows: 1. Of 54 patients, 34 patients were low stage(0-A) and low grade(1-2). 2. There is a significant correlation between tumor grade and SRCA test: Of 38 patients with low grade. 19 patients were SRCA positive, but of 16 patients with high grade. all were SRCA negative. 3. There is a significant correlation between tumor stage and SRCA test: Of 36 patients with low stage, 18 patients were SRCA positive, but of 18 patients with high stage(above B1), only one patient was SRCA positive. 4. There is a high possibility of false-negative results in detecting O(H) isoantigen: Of 36 patients with low stage, 6 patients were blood group 0 who were all SRCA negative. but 30 patients with other blood groups showed variable SRCA results. 5. There is a considerable correlation between tumor recurrence and SRCA result: Of 20 patients who were followed more than one year after initial TUR, 8 patients were SRCA positive, of these 4 patients were recurred, but 9 patients of 12 patients with SRCA negative were recurred.
Antigens, Surface* ; Blood Group Antigens ; Humans ; Isoantigens ; Recurrence ; Urinary Bladder*

In addition to the author's previous report (1960), a further analysis was made with more blood samples and more varieties of antisera. Those data with Rh-Hr, Kell, Duffy, MN systems and subgroups in the ABO system were compared with the previous data. As to some of the new antisera such as Cellarlo, Kpa, Kpb, S, s, P, Diego, Lutheran and Lewis, the first analysis on Korean blood samples was made of this time. The data were compared with the data on other racial groups as observed by others. In general the present data of Rh-Hr system confirmed our previous findings. The most frequent cell types were Rh1 Rh2 (CcdEe) and Rh1 Rh1(CDe). The frequency of rh (cde) cell was one in 332 or 0.3%. In addition to the eight phenotypes which were encountered in the author's (1960) previous series, two rare types were found in this study. Still two other phenotypes were identified in an Rh0(D) negative family's family-study. The close association of gene Rz (CDE) with Asiatic races was discussed. Kell factor seems even rarer than it was thought. Cellano and and Kpb (Rautenberg) antigens appeared to be prevalent in Koreans while Kpa (Penny) antigen appeared rare in Koreans as was the Kell factor. The Duffy factor seems more frequent than it was thought. The S-factor was relatively low in Koreans as compared with the English. It seemed more associated with the N factor than with the M factor. The s-factor was almost universal in Koreans. The rarity of the A2 and A2B Cell was again demonstrated. The frequency of the P-factor was lower than that found in the English and higher than that of the Chinese and Japanese. The Diego factor was certainly present in Korean blood samples and the frequency was even higher than that found in the Japanese as reported by others. Out of random blood samples of 117 Koreans studied, 17were found positive, a positivity of 14.5%. No Lu (a+) blood was found in 95 random samples and apparently Lu(b) is universal in Koreans. The frequency of Le (a+) was essentially the same range as in the English.
Asian Continental Ancestry Group ; *Blood Group Antigens ; Human ; Korea

There were three new blood group systems including the KANNO blood group system, the Sid blood group system and the CTL2 blood group system (provisional status), have been registered by the International Society of Blood Transfusion (ISBT) registered Science August 2019. The main reason for this update is that the significant SNPs of the KANNO blood group system (rs1800014) and the Sid blood group system (rs7224888) have been found through genome-wide association studies and whole exome sequencing. The new genetic evidences are consistent with the current immunological findings. In addition, although CTL2 antigen has been found on erythrocyte ghost (erythrocyte membrane) since 2017, CTL2 blood group system is still in provisional status due to lack of serological and genetic evidence. In this review, the experimental research advances of these three ISBT blood group systems and discuss the clinical value of the relevant researches was summarized briefly.
Blood Group Antigens ; Blood Transfusion ; Genome-Wide Association Study ; Humans

OBJECTIVE: To explore the causes and specific conditions of blood donation reaction under the collective emergency unpaid blood donation, and to provide theoretical basis and decision-making reference for drafting the collective emergency unpaid blood donation and blood donation safety. METHODS: Through a combination of prospective and retrospective models, and statistical methods were used to analyze the causes and conditions of the blood donation response of 10401 people participating in collective emergency unpaid blood donation during 2016.1-2018.8. RESULTS: A total of 10401 person-times donated blood in a sitting manner, and a total of 293 blood donation reactions occurred. By improving the blood donation services year by year, the moderate blood donation reaction during the year 2017 and 2018 was significantly lower than that in 2016 (P＜0.05). In the actual blood donation group of≤100, 200, 300 and 400 ml, the incidence of blood donation reaction was statistically significant (P＜0.05); the incidence of blood donation reaction in the blood donors for 1,2,3 and ＞3 drnations was also statistically significant (P＜0.05); the blood donation reactions rate of B antigen containers was significantly different from the donors without B antigen (P＜0.05); the incidence of blood donation reaction with related to the weight of the donor. CONCLUSION The blood donation reaction of collective emergency unpaid blood donation closely relates with mental factors, blood donation service, blood donation frequency and body weight of the blood donor. The first blood donation is more likely to produce blood donation reaction. The blood donation volum≤ 100 ml from blood donors is resulted mostly from blood donation reactions.
Blood Donors ; Blood Group Antigens ; Humans ; Prospective Studies ; Retrospective Studies