Aims: The present study is aimed at taxonomic characterization and isolation of active compound MS01 from Streptomyces sp. FACC-A032 which exhibited strong antitrypanosomal activity (IC50 0.02 μg/mL). Methodology and results: Isolate FACC-A032 was characterized based on its cultural, morphological, physiological and genomic properties. Isolate FACC-A032 was tentatively identified as Streptomyces sp. Biochemical analysis of diaminopimelic acid (DAP) isomer of whole-cell hydrolysates further confirmed the isolate FACC-A032 that contained LL-DAP isomer as species belonging to the genus Streptomyces. The inoculum for submerged cultures of isolate FACCA032 was prepared from cultures on ISP2 agar. After eight days of growth at 28  2 °C and 200 rpm in fermentation medium M3, fermentation broth was extracted with butanol and the crude extracts (solvent layer) were separated and dried in vacuo. Further studies were carried out to isolate the active compound from the culture extracts of isolate FACCA032. Using bioassay-guided isolation, crude extract was partitioned based on different polarity. After which, the resulting elutes were tested for antitrypanosomal activity. The active fraction was analyzed with HPLC-DAD analysis. Based on the analysis, major peak in the active fraction was collected using HPLC preparative. Active compound MS01 was isolated and structure elucidated using NMR spectroscopy. Conclusion, significance and impact of study: Bioassay-guided isolation techniques used in this study had discovered an active antitrypanosomal compound, staurosporine, from Streptomyces sp. FACC-A032. This is the first discovery of staurosporine, a protein kinase inhibitor, from Malaysian soil actinobacteria Streptomyces sp. Therefore, the study demonstrated the potential of Malaysian soil actinobacteria as antitrypanosomal therapeutic agent.
Biological Assay ; Actinobacteria

Subjects: Normal people without the benign or malignant tumor; standard sample of CA72-4 with concentrations: 0,3; 25; 50 100 u/ml. The results have shown that the standard chart has relatively same as theory; the change factor CV had value within allowed limit in the range of low concentration of CA 72-4 was 4,05+/- 3,06U/ml. The medium value was 4,92 u/ml. The normal range is from 0 to 9 u/ml. There is insignificant different between women and men.
Neoplasms ; Immunoradiometric Assay ; Tumor Markers, Biological

This study described methods to predict human health risk associated with exposure to environmental carciongens using animal bioassay data. Also, biological assumption for various dose-response models were reviewed. To illustrate the process of risk estimate using relevant dose-response models such as Log-normal, Mantel-Bryan, Weibull and Multistage model, we used four animal carcinogenesis bioassy data of chloroform and chloroform concentrations of tap water measured in large cities of korea from 1987 to 1995. As a result, in the case of using average concentration in exposure data and 95 % upper boud unit risk of Multistage model, excess cancer risk(RISK I) was about 1.9 x 10-6, in the case of using probability distribution of cumulative exposure data and unit risks, those risks(RISK II) which were simulated by Monte-Carlo analysis were about 2.4 x 10(-6) and 7.9 x 10(-5) at 50 and 95 percentile, respectively. Therefore risk estimated by Monte-Carlo analysis using probability distribution of input variables may be more conservative.
Animals ; Biological Assay ; Carcinogenesis ; Carcinogens, Environmental* ; Chloroform ; Humans ; Korea ; Water

It has been well established that the kidney is the main organ producing erythropoietin (E.S.F.). Erythropoietin activity was reported to be increased in cases of renal tumor. cyst, polycystic disease and hydronephrosis. To study erythropoietic activity in hydronephrosis and renal cell carcinoma, erythropoietin bioassay by DeGowin method were performed in 7cases of hydronephrosis and renal cell carcinoma. Erythropoietin activity is significantly increased in renal cell carcinoma but not significantly increasedin hydronephrosis.
Biological Assay* ; Carcinoma, Renal Cell* ; Erythropoietin* ; Hydronephrosis* ; Kidney

OBJECTIVE: The aqueous and pelletized admixture of Piper nigrum L. was evaluated for its oviposition response and larvicidal activity against Aedes aegypti mosquitoes.

METHODS: The aqueous and pelletized extract of Piper nigrum L. was prepared and first tested in the laboratory. Efficiency is evaluated using the mosquito-chamber test. A small-scale field test was also done to determine the oviposition response of the pepper extract to ovicidal-larvicidal (OL) traps. Larvicidal bioassay following the WHO standard protocols with slight modification at different concentrations was performed.

RESULTS: Results of the mosquito chamber test in the laboratory showed that the aqueous solution exhibited an increasing rate of oviposition attraction of female Aedes aegypti to increasing rate of concentration with an average of 70% attraction at 1000 ppm as compared to 30% attraction to OL traps with water alone. The aqueous pepper-based solution and pelletized pepper solution at 1000 and 2000 ppm are considered attractants to Aedes mosquitoes. Both solutions have oviposition activity index (OAI) of > +0.3. It was also field tested on the 10 buildings within the Department of Science and Technology (DOST) compound. Results showed an oviposition ratio two times better in both the 1000 ppm concentration of the aqueous pepper-based solution and pelletized pepper solution as compared to the control. The positive ovitrap index was in the range of 78%-84% for both the aqueous pepper-based and pelletized pepper against water which is 70.0%. Larvicidal activity of the aqueous pepper-based solution against 3rd larval instars of Aedes aegypti at increasing dosages from 75mg/1 to 600 mg/I had an LCso of 127 mg/I and 395 mg/I for LC90 The LCso for the solution with pelletized pepper at the same dosing concentration is 117 mg/I with LC90 of 285 mg/1. The results also showed that these can be used to control larval instars of the Aedes aegypti mosquitoes.

CONCLUSION: The overall results indicate that the aqueous and pelletized extracts of Piper nigrum L. are effective in attracting the Aedes aegypti mosquitoes for oviposition and exhibit a larvicidal activity.

Six new ent-abietane diterpenoids, abientaphlogatones A-F (1-6), along with two undescribed ent-abietane diterpenoid glucosides, abientaphlogasides A-B (7-8) and four known analogs were isolated from the aerial parts ofPhlogacanthus curviflorus (P. curviflorus). The structures of these compounds were determined using high-resolution electrospray ionization mass spectrometry (HR-ESI-MS), one-dimensional and two-dimensional nuclear magnetic resonance (NMR) spectroscopy, electronic circular dichroism (ECD) spectra, and quantum chemical calculations. Notably, compounds 5 and 6 represented the first reported instances of ent-norabietane diterpenoids from the genus Phlogacanthus. In the β-hematin formation inhibition assay, compounds 2, 4, 7-10, and 12 displayed antimalarial activity, with IC₅₀ values of 12.97-65.01 μmol·L^-1. Furthermore, compounds 4, 5, 8, and 10 demonstrated neuroprotective activity in PC12 cell injury models induced by H₂O₂ and MPP⁺.
Abietanes/pharmacology* ; Antimalarials ; Hydrogen Peroxide ; Biological Assay ; Plant Components, Aerial

This paper aimed to study the chemical constituents from the root bark of Schisandra sphenanthera. Silica, Sephadex LH-20 and RP-HPLC were used to separate and purify the 80% ethanol extract of S. sphenanthera. Eleven compounds were identified by ~1H-NMR, ~(13)C-NMR, ESI-MS, etc., which were 2-[2-hydroxy-5-(3-hydroxypropyl)-3-methoxyphenyl]-propane-1,3-diol(1), threo-7-methoxyguaiacylglycerol(2),4-O-(2-hydroxy-1-hydroxymethylethyl)-dihydroconiferylalcohol(3), morusin(4), sanggenol A(5), sanggenon I(6), sanggenon N(7), leachianone G(8),(+)-catechin(9), epicatechin(10), and 7,4'-dimethoxyisoflavone(11). Among them, compound 1 was a new compound, and compounds 2-9 were isolated from S. sphenanthera for the first time. Compounds 2-11 were subjected to cell viability assay, and the results revealed that compounds 4 and 5 had potential cytotoxicity, and compound 4 also had potential antiviral activity.
Schisandra ; Plant Bark ; Antiviral Agents ; Biological Assay ; Catechin ; Phenols

Asians ; Biological Assay ; Breast Neoplasms/surgery* ; China ; Female ; Humans ; Mastectomy

Androgens remain the most effective and widely abused ergogenic drugs in sport. Although androgen doping has been prohibited for over 3 decades with a ban enforced by mass spectrometric (MS)-based urine testing for synthetic and exogenous natural androgens, attempts continue to develop increasingly complex schemes to circumvent the ban. A prominent recent approach has been the development of designer androgens. Such never-marketed androgens evade detection because mass spectrometry relies on identifying characteristic chemical signatures requiring prior knowledge of chemical structure. Although once known, designer androgens are readily detected and added to the Prohibited List. However, until their structures are elucidated, designer androgens can circumvent the ban on androgen doping. To combat this, in vitro androgen bioassays offer powerful new possibilities for the generic detection of unidentified bioactive androgens, regardless of their chemical structure. Another approach to circumvent the ban on androgen doping has been the development of indirect androgen doping, the use of exogenous drugs to produce a sustained increase in endogenous testosterone (T) production. Apart from estrogen blockers, however, such neuroendocrine active drugs mostly provide only transient increases in blood T. Finally the ban on androgen doping must allow provision for rare athletes with incidental, proven androgen deficiency who require T replacement therapy. The Therapeutic Use Exemption mechanism makes provision for such necessary medical treatment, subject to rigorous criteria for demonstrating a genuine ongoing need for T and monitoring of T dosage. Effective deterrence of sports doping requires novel, increasingly sophisticated detection options calibrated to defeat these challenges, without which fairness in sport is tarnished and the social and health idealization of sporting champions devalued.
Androgens ; administration & dosage ; Biological Assay ; Designer Drugs ; Doping in Sports ; Humans

Selection of fungal strains with high virulence against the developmental stages of Bemisia tabaci was performed using internal transcribed spacer regions. The growth rate of hyphae was measured and bioassay of each developmental stage of B. tabaci was conducted for seven days. All of the fungal strains tested were identified as Lecanicillium spp., with strain 4078 showing the fastest mycelium growth rate (colony diameter, 16.3 +/- 0.9 mm) among the strains. Compared to strain 4075, which showed the slowest growth rate, the growth rate of strain 4078 was increased almost 2-fold after seven days. Strains 4078 and Btab01 were most virulent against the egg and larva stages, respectively. The virulence of fungal strains against the adult stage was high, except for strains 41185 and 3387. Based on the growth rate of mycelium and level of virulence, strains 4078 and Btab01 were selected as the best fungal strains for application to B. tabaci, regardless of developmental stage.
Adult ; Biological Assay ; Humans ; Hyphae ; Larva ; Mycelium ; Ovum ; Sprains and Strains