Aim To investigate a possible role for nitric oxide and neurogenic pathway in the protective effect of the limb preconditioning on the ischemic-reperfusion myocardium.Methods 64 Wistar rats were randomly divided into one of the four experimental groups.In Group Ⅰ,the rats underwent 30 min occlusion of the left anterior descending coronary artery,and 120 min reperfusion.In Group PL,the rats underwent four cycles of 5 min occlusion and reperfusion of both hind limbs using a tourniquet before the experiment was continued as in Group Ⅰ.In Group PL-N and Group PL-H,rats were administered with L-Nitro-Arginine Methyl Ester(L-NAME)10 mg?kg-1 or hexamethonium chloride 20 mg?kg-1,intravenously,20 min before IPC.Infarct size,as a percentage of the area at risk,was determined by triphenyltetrazolium chloride staining.And other 8 rats in each group,at the end of the experiment,all rats were killed and myocardium were stored in liquid nitrogen for the measurement of NO,NOS,iNOS and iNOS mRNA.Results The myocardial infarct size(IS)was decreased significantly in Group PL and Group PL-H compared with Group Ⅰ(P

Objective To investigate the postoperative analgesia efficacy of ultrasound-guided rectus abdominis sheath block (RSB) in patients with abdomen panhysterectomy.Methods Forty patients with abdomen panhysterectomy under general anesthesia were divided into RSB group and control group by random digits table method with 20 cases each.All the patients were given patient controlled intravenous analgesia.The visual analogue scale (VAS) score and Ramsay sedative score at 2,6,12 and 24 h after operation,sulfentanyl dosage and total number of analgesic pump press of 24 h,number of nausea and vomiting,analgesic satisfaction score were recorded.Results The VAS scores in RSB group at 2,6,12,24 h after operation were lower than those in control group,but there were no statistical differences (P＞ 0.05).The Ramsay sedative score of all patients was ＜ 4 scores.Sulfentanyl dosage of 24 h in RSB group was (73.1 ± 8.6) μ g,in control group was (91.7 ± 12.3) μ g,there was statistical difference (P ＜ 0.01).The number of analgesic pump press of 24 in RSB group was (6.5 ± 2.1) times,in control group was (12.3 ± 4.6)times,there was statistical difference (P ＜ 0.01).Analgesic satisfaction score in RSB group was (7.3 ± 0.7)scores,in control group was (6.8 ± 1.1) scores,there was statistical difference (P＜ 0.05).Four cases in RSB happened nausea and vomiting,3 cases in control group,there was no statistical difference (P ＞ 0.05).All patients did not happen the complication related with RSB operation.Conclusion RSB as the postoperative analgesia in patients with abdomen panhysterectomy can reduce the dosage of opioid drug,and increases the analgesic satisfaction.

Blood Gas Analysis ; Humans ; Infant, Newborn ; Neuromuscular Nondepolarizing Agents ; therapeutic use ; Respiration, Artificial ; adverse effects ; Vecuronium Bromide ; therapeutic use

Objective:To evaluate the clinical efficacy of Sanjiao Cidi therapy on acute cerebral infarction and its effect on levels of S100-β protein （S100-β）， tumor necrosis factor-α （TNF-α）， hypersensitive C-reactive protein （hs-CRP） and neuropeptide （NPY）. Method:One hundred and eighty patients were randomly divided into control group （90 cases） and observation group （90 cases） by random number table. Patients in control group got aspirin enteric-coated tablets， 100 mg/time， 1 time/day， edaravone injection （injected within 30 minutes） for 14 days， 30 mg/time， 2 times/day， simvastatin tablets， 20 mg/time， 1 time/day. In addition to the basic therapy of meloxicam tablets， patients in observation group were also treated with Sanjiao Cidi therapy. In the first step， patients got Guizhi therapy to dredge Zhongjiao and Shangjiao， 1 dose/day， for 8 days. In the second step， patients got Sini therapy to dredge Zhongjiao and Xiajiao， 1 dose/day， for 10 days. In the third step， patients got Tianjing Gubentherapy， 1 dose/day， for 10 days. The course of treatment was 4 weeks. Before the treatment， and at the first， second， third and fourth weeks after treatment， National Institutes of Health Stroke Scale （NIHSS） was scored. And before and after treatment， function scale of fuglmeyer （FMA）， ability of daily life activities （ADL）， mini-mental state examination （MMSE） and main symptoms of traditional Chinese medicine were scored. Comprehensive assessment of patient report outcome （PRO） was made. And levels of S100-β， hs-CRP， TNF-α and NPY were detected. And the incidence rate of pulmonary infection， urinary infection， skeletal myalgia， shoulder hand syndrome and shoulder subluxation of patients were recorded during hospitalization. Result:The clinical efficacy in observation group was better than that in control group （Z=2.141， P<0.05）. Scores of NIHSS in observation group were lower than those in control group at the first， second， third and fourth weeks after treatment （P<0.01）. Scores of upper limb， legs and the total scores from FMA were higher than those in control group （P<0.01）. Scores of the main symptoms of traditional Chinese medicine， symptoms， psychological and social scores， total scores of PRO， S100-β， hs-CRP， TNF-α and NPY were lower than those in the observation group （P<0.01）. And scores of ADL and MMSE were higher than those in control group （P<0.01）. Total incidence of complications in observation group was 27.27%（21/77）， which was lower than 46.15%（36/78） in control group （χ²=5.941， P<0.05）. Conclusion:In addition to conventional western medicine treatment， Sanjiao Cidi therapy can treat the patients with acute cerebral infarction， alleviate the degree of neurological deficit， improve the cognitive function， motor function of limbs and the ability of daily life， reduce the main symptoms of traditional Chinese medicine， the incidence of complications and the inflammatory response， protect the nerve cells， with a better clinical efficacy and comprehensive effect in patients than pure Western medicine.