Objective To observe the effect of inhibition of polo like kinasel (Plk-1) gene expression on 5-Fu and CDDP induced apoptosis of gastric cancer cell line-MKN45 in vitro. Methods The Plk-1 expression was inhibited by chemically synthesized siRNA, the Plk-1 mRNA and protein level were measured by real-time PCR and Western blotting, MKN45 cells proliferation was measured by MTT method, the apoptosis rate was detected by flow-cytometry. Results After treatment by siRNA targeting Plk-1, Plk-1 mRNA level decreased by 51.91% .89.45%.91.03% at 24 h.48 h and 72 h, and Plk-1 protein level decreased by 38.43% and 60.66% at 48 h and 72 h, compared with control group. The proliferation of MKN45 cells treated by a combination of 5-Fu, CDDP and Plk-1 siRNA significantly slowed down when compared with treatment of 5-Fu, CDDP or Plk-1 siRNA(P

Objective? To explore the effects of scenario simulation combined with case share on fall prevention education in elderly patients. Methods? A total of 80 elderly patients admitted in Shaanxi Provincial People's Hospital from January 2016 to December 2017 were selected by convenient sampling and equally divided into two groups according to the random number table. Patients in the control group received conventional fall prevention education, while patients in the observation group received fall prevention education which combined scenario simulation and case share. Falls of patients, effects of education and satisfaction were compared between the two groups. Results? The incidence rate of falls in the observation group during hospitalization was 10.0%, while that in the control group was 20.0% (χ2=4.325; P＜0.05); the knowledge of fall prevention, the awareness of fall prevention and fall prevention compliance scored (86.3±5.2), (84.2±5.3) and (91.3±8.4), higher than those in the control group (t=9.981, 9.040, 12.872; P＜ 0.01), which were (76.3±4.4), (75.2±3.4) and (71.3±5.1) respectively. The satisfaction with nursing in the observation group was 95.0%, higher than that in the control group (χ2=4.201; P＜0.05). Conclusions? Fall prevention education which combines scenario simulation and case share can effectively enhance the awareness of fall prevention, reduce the incidence of falls and improve the satisfaction with nursing in the patients, which is worth promoting in clinical practice.

Objective To evaluate the impact of the professional knowledge and skills of hospital attendants on the quality of hospital care and its training effect. Methods200 accompanists from a Class ⅢGrade A hospital in Xi'an from January 2017 to December 2018 were selected as the subjects by convenient sampling. 100 accompanists from January to December 2018 were used as observation groups to develop their professional knowledge and skills training; 100 accompanists who did not carry out professional knowledge and skills training during from January to December 2017 were the control group. The professional knowledge and skills, quality of care were compared between the two groups before and after training. ResultsThe scores of basic care, daily life care, psychological care, functional rehabilitation, cleansing care, and communication in the observation group were higher than those in the control group, and the differences between the two groups were statistically significant (P＜ 0.05). After training, the patients' health education awareness rate, complication rate, safety incident rate, and satisfaction were better than before training, and the differences were statistically significant (P＜0.05). ConclusionsDue to the limitation of professional knowledge and skill level, the comprehensive quality of hospital accompanists needs to be improved. Strengthening the training of accompanists can not only improve the comprehensive quality of the accompanists themselves, but also improve the quality of hospital care, which is beneficial to the health and rapidity of the medical care industry.