AIM To investigate the mices central nerve system function and the effect of drugs through independent activity pattern observation. METHODS By observing the influence of drugs on the independent activity patterns of mice of different testing time and testing times, sex, sound, and brightness. RESULTS The mices independent activity is related to testing times, sound and drugs applied, but not related to testing interval, sex, and brightness. CONCLUSION Individual difference of the independent activity of mice is great. The quietness of the experimental environment influences the result.