100 cases of patients with brain tumor were tested with lymphocyte transformation testand erythrocyte-rosette forming cell,the results showed the lymphocyte transfor-mation test and Erythrocyte-Rosette Forming Cell of the patients with brain tumorwas lower than that of the normal value,of the 100 postoperative brain tumor cases75 were treated with blood-enriching decoction of Angelica Sinensis and 25 were usedas control.The lymphocyte transformation test and erythrocyte-Rosette formingcell of the treated group showed a remarkable rise(P

Objective To investigate the disease occurrence-related factors and severity of alcoholic hepatitis (AH) and alcoholic liver cirrhosis (ALC).Methods According to the clinical diagnostic criteria that were passed by the 2010 Chinese Society of Fatty Liver Hepatology and alcoholic liver disease study group,the clinical data of 241 AH and ALC patients were analyzed.Results The amount of liquor taken daily,the time limit of drinking,and total amount of liquor taken in ALC patients were significantly different from those in AH patients (P ＜0.01),respectively.In ALC patients,the proportion (48.39％)of workers,peasants,and unemployed low-income alcoholics was significantly higher than that (25.58％)in AH patients (P ＜0.01).Laboratory tests showed the AST/ALT ratio in the ALC group (2.63 ±4.74)was significantly higher than that in the AH group (1.01 ±0.52) (P ＜0.01); TG [(1.33±1.24)mmol/L] and cholesterol (CHOL) [(3.14 ± 1.25) mmol/L] in ALC group were significantly lower than TG [(2.29 ± 1.60)mmol/L] and CHOL [(4.27 ±0.79)mmol/L] in the AH group (P ＜0.01).Conclusions Alcoholic liver disease occurrence and severity were related to the amount of liquor taken,the time limit of drinking,and the work ; AST/ALT ratio,TG and CHOL values were consistent with the severity of alcoholic liver disease.

Bone Diseases, Metabolic ; diagnosis ; etiology ; therapy ; Chronic Disease ; Humans ; Liver Diseases ; complications ; diagnosis ; therapy

Objective To study the relationships of fatty liver with the waist to hip ratio (WHR),obesity,blood glucose,blood lipid,so as to guide clinical treatment.Methods A total of 5827 persons who underwent the following examination,including liver function,kidney function,blood lipids,blood glucose,and ultrasound imaging examination were divided into fatty liver suffering group and non-fatty liver suffering group.Results The incidences of WHR,diabetes,hyperlipidemia,and hyperuricemia in the fatty liver suffering group were significantly higher than those in the non-fatty liver suffers group(t =24.6,13.2,21.9,19.6,10.3,P ＜ 0.01).The incidence (44.3％) of fatty liver in males was significantly higher than that (31％) in females (x2 =33.6,P ＜ 0.01).Conclusions There was obvious correction between fatty liver and abdominal obesity.Patients with fatty liver should be given early prevention in order to prevent from the occurrence of cardiovascular events and metabolic syndrome.

Objective To observe the effects of hydrogen sulfide on hepatic and renal injury induced by thioacetamide (TAA).Methods Twenty-four male SD rats were equally and randomly divided into TAA induced model group ,control group,TAA +sodium hydrogen sulfide group , and TAA +propargylglycine group .TAA was given by intraperitoneal injection to the model group , sodium hydrogen sulfide and propargylglycine groups at the dose of 600 mg/kg on the first day, and at the dose of 300 mg/kg 24 hours later.Hydrogen sulfide sodium at the dose of 0.15 mmol/kg and propargylg-lycine at the dose of 30 mg/kg were injected abdominally 1 h before TAA injection.The dose of these two agents was halved in the next 24 h.All the rats were sacrificed and specimens were collected to test hydrogen sulfide , and hepatic and renal function.Urine after the first 24 hours was collected after the administration of TAA .Results The structure of the liver in TAA group was disordered , especially in sodium hydrogen sulfide group .Liver function of TAA group was severely damaged (ALT:980.0 ±32.0 vs 38.3 ±10.6 U/L),and deteriorated with the application of sodium hydrogen sulfide (ALT:1095.6 ± 684.2 U/L), but recovered (ALT:66.3 ±8.32 U/L) with propargylglycine application.Compared with the normal group, the renal function in TAA group was significantly injured ,but the urea nitrogen and creatinine in sodium hydrosulfide group were reduced more remarkably than in TAA group (BUN:8.4 ±1.9 vs 11.62 ±6.0 mmol/L,Cr:32.6 ±8.2 vs 42.8 ±4.4 μmol/L, P<0.05).Nevertheless, creatinine was increased in propargylglycine group ( Cr 56.7 ±14.9 vs 30.8 ±4.4μmol/L, P<0.05).The urine content within 24 hours was more significantly decreased in TAA group than in the normal group (9.2 ±2.5 vs 20.5 ±6.7 ml, P<0.01), which was mildly recovered in sodium hydrogen sulfide group (11.7 ± 1.5 vs 9.2 ±2.5 , P<0.05) but further decreased (4.2 ±1.3 vs 9.2 ±2.5, P<0.01) in the propargylglycine group . Conclusion Hydrogen sulfide can alleviates renal injury while aggravating liver injury induced by thioacetamide .

Objective Through studying the apoptosis induced by stichopus japonicus acid mucopoly saccharide in the hepatocellular carcinoma cell line HepG2 in vitro, analysing the expression of Bcl-2 and nm-23in HepG2, to provide the theory foundation and its feasibility on whether it can be used for the chemotherapy of hepetocellular carcinoma. Methods The cells of HepG2 were cultured in vitro and treated with SJAMP at different doses(0.25,0. 5,1.0,2.0,4.0 g/L). MTT was used to observe the inhibitory effects of SJAMP on cell growth, Western blotting was used to detect apoptosis, and the apoptosis related change of expression of protein Bcl-2 and nm23-H1. Results (1) MTT identified that SJAMP produced an obvious time-and-dose-dependent inhibitory effect on the HlepG2 cells. (2) Western blot showed that SJAMP could induce the apoptosis of HepG2 cells through changing the expression of the protein of Bcl-2 and nn23-H1 (P<0.05). Conclusion (1)SJAMP produced obvious inhibitory effects on HepG2 cells and induce HepG2 apoptosis. (2)SJAMP can enduce the anti-tumor function in the method of changing the expression of protein Bcl-2 and nm23-H1.

Objective To provide reference for effective scientific literature novelty assessment by investigating its recognition in medical and health staff of a military area command and their use of information resources and need of information .Methods Knowledge of scientific literature novelty assessment , satisfaction of scientific literature novelty assessment report, need and use of information resources were investigated in 149 staff of 9 hospitals with self-de-signed questionnaire .Results The general recognition and satisfaction levels of scientific literature novelty assess-ment were good in medical and health staff of a military area command .However , the information resources were insufficiently used , which was related with the scientific research level and scientific literature novelty assessment popularization .Conclusion Scientific literature novelty assessment should be popularized , staff engaged in scientific literature novelty assessment should be trained, sharing of information resources should be strengthened , and infor-mation service should be expanded and deepened in military area commands.

Adult ; Bone Diseases, Metabolic ; etiology ; Female ; Fractures, Spontaneous ; etiology ; Humans ; Liver Diseases, Alcoholic ; complications

Objective To explore pathogenic bacteria distribution and drug susceptibility testing results in children with purulent meningitis in Jinan area. Methods A total of 54 children with purulent meningitis were selected from January 2010 to December 2014, the cerebrospinal fluid smear and culture, according to the national standard of clinical inspection technology for bacteria isolation and identification by disc diffusion method for drug sensitive test were retrospectively analyzed. Results A total of 54 strains of pathogenic bacteria including 36 strains of gram-positive coccus, and 17 strains of gram-negative bacillus and one strain of suspected Neisseria meningitides were found. A total of 31 strains gram positive coccus is Streptococcus pneumoniae, and most gram-negative bacilli is E. coli. In the Gram-positive coccus, 61.3% was sensitive to penicillin, and more than 90% was sensitive to ceftriaxone and cefepime, 83.3% was sensitive to meropenem, 94.7% resistant to azithromycin, and 58.1% resistant to oxazocilline. In Gram-negative bacilli, 60% was sensitive to ampicillin sulbactam 71.4% was sensitive to cephalosporin , 57.1% was sensitive to ceftriaxone , 66.6% was sensitive to cefepime. Conclusions From cerebrospinal fluid cultured of purulent meningitis, Streptococcus pneumoniae and E. coli were major pathogenic bacteria in children with purulent meningitis in Jinan area.

Objective To investigate expression of slug and E-cadherin in pancreatic cancer tissues and determine the inhibitory effects of anti-Slug, an anti-sense plasmid, on the invasion of pancreatic cancer cell lines in vitro. Methods Slug and E-cadherin protein and mRNA was analyzed by IHP and RT-PCR in 36 cases of pancreatic cancer. Then anti-Slug plasmid was transfected into herin and Slug expression. The inhibitory effects of anti-sense Slug were also detected by Transwell motility assay and Matrigel invasion assay. Results The expression of Slug and mRNA in metastatic pancreatic cancer tissue was higher than that in non-metastatic tissue. E-cadherin and mRNA was lower in metastasis tissues(P＜0.05). The inverse relationships were further observed by transient transfection of anti-Slug into SW1990H4 cells. The downregulated expression of Slug and re-expression of E-cadherin were found. The Slug mRNA levels were 0.985±0.016,0.973±0.014, 0. 554±0. 011 after 0, 48 h of transfection of anti-sense Slug, and that of E-cadherin were 0.120±0.001, 0.360±0.002, 0. 727±0. 006, respectively. The diference was significant between different time points (P＜0.05). The Slug mRNA levels were 0. 206±0.017, 0.968±0.015, and that of E-cadherin were 0. 18±0.002,0.727±0.006 after stable transfection of anti-sense Slug, and control plasmid, respectively. The diference was significant (P＜0.05). The motility activity(393±28, 352±24, 96 ±13 )and the invasion activity (223 ± 69, 202 ± 64, 65 ±19) of1 antisense Slug transfectant cells were significantly decreased as compared with those of control cells (P＜0.05). Conclusions Higher expression of slug and lower expression of E-cadherin is related to the invasion and metastasis in pancreatic cancer. A reverse corelation of E-cadherin and Slug expression exists in pancreatic cancer. Slug is possibly a potential target for cancer gene therapy blocking invasion and metastasis in human pancreatic cancer.