1.Prevalence and Risk Factors of Illness within Two-week among Rural Residents in Menglian:a Multilevel Model Analysis
Yong MAO ; Long CHEN ; Jia ZHOU ; Tao WEI ; Bingxian QI
Journal of Kunming Medical University 2013;(10):47-52
Objective To assess prevalence and risk factors of illness within the past 2 weeks among rural residents in Menglian,under the background of essential public health service implemented generally. Methods In February 2012, the questionnaire method was employed to investigate the two-week morbidity in 2011 rural residents sampled by multistage stratified random sampling from Menglian population. Results The prevalence, that of illness within two-week among 2011 rural residents in Menglian, was 97.5‰ (95%CI 84.5‰-110.5‰) . It was lower than that of the rural region IV in China (149.6‰, <0.05) .Its age trend was the same as that of the rural region IV in China,the prevalence in 0-14 years old children,however,was higher than the counterpart in the rural region IV in China. The diseases, which two-week morbidity ranked the top five, were acute upper respiratory infection, acute gastroenteritis, hyperostosis, acute tracheobronchitis and rheumatoid arthritis. The results of multilevel logistic regression model fitted by group-level explanatory variable mountainous area, individual-level random slopes variable gender and other individual background variables indicated that there were across-level interactions between mountainous area and gender ( <0.05), the family income lower than RMB 15000 (odds ration 3.2378, 95%CI 1.9014-5.5130) and age (odds ration 1.0163, 95%CI 1.0002-1.0327) had a positive effect to two-week morbidity, and contrasting to unmarried, married had a negative effect to two-week morbidity (odds ratio 0.4727, 95%CI 0.2534-0.8819) . Conclusions Comparing with that of the rural region IV in China before implementing essential public health service, the two-week morbidity in Menglian was lower. For further elevating the health level of population, the strategies, such as intensifying material and child hygiene in the mountain area, improving sanitary conditions of low income family, and updating health consciousness in single population,would be implemented.
2.Implementation and Influencing Factors of Essential Public Health Services in Menglian
Jia ZHOU ; Yong MAO ; Long CHEN ; Tao WEI ; Bingxian QI
Journal of Kunming Medical University 2013;(10):35-38,57
Objective To assess the implementation of essential public health services (EPHS), and determine the main influencing factors for EPHS in Menglian. Methods In September 2012, the questionnaire survey method was employed to collect the data of EPHS implementation in 2011 in three community medical institutes and the EPHS evaluation of health staff sampled by stratified random sampling in Menglian. Results In 2011, the report rates of infectious diseases epidemics, public health emergencies and health inspection were all 100%, the inoculation rates of most vaccines were over 90%,and the health management rates of the children aged 0 to 6 years,pregnant and lying-in woman,aged population,hypertensives, type 2 diabetes patients and serious psychotics were high (about 85%) . The establishment rate of heath archives (60%to 70%),the controlling rates of blood pressure in the hypertensive population (about 50%), the rates of glycemic control in type 2 diabetes patients (55%to 70%) and the steady rates of serious psychotics (50% to 60%), however, were low. The implementation of EPHS was unbalance among towns, suburbs and outer suburbs. The main factors that influenced the EPHS implementation were inadequate human resources, insufficient or unused health devices, ambiguous responsibilities among the health institutes, non-cooperative behaviors, and unhealthy living habits in rural residents. Conclusions The implementation of many EPHS items is good. For promoting EPHS,it is necessary to train human resources,activiate unused health devices,get support of residents and carry out health education.
3.Detection rate of hypertension in elderly patients of Deang nationality and multilevel model of its influencing factors
Yong MAO ; Jia ZHOU ; Tianshu CHU ; Songmei WANG ; Chenghuan SUN ; Jiahong LUO ; Chunmi LAI ; Bingxian QI ; Qian ZHANG
Chinese Journal of Geriatric Heart Brain and Vessel Diseases 2018;20(1):16-19
Objective To study the influencing factors of primary hypertension by investigating its prevalence in elderly patients of Deang nationality.Methods Nine hundred and thirty-nine elderly patients of Deang nationality were divided into hypertension group (n =353),prehypertension group (n=377) and control group (n=209).Their physical examination data were recorded and their blood pressure was measured.Results The detection rate of hypertension was significantly lower in elderly patients of Deang nationality than in those of the other nationalities in our country with their age ≥60 years (37.6% vs 49.0%,P<0.05).The detection rate of hypertension,isolated systolic hypertension and prehypertension increased with the increasing age (P<0.05).Multivariate logistic regression analysis showed a cross interaction between economic level and alcohol drinking (P<0.05) and a positive correlation of BMI,age,gender,divorce or widow,smonking,high salt diet with hypertension (P<0.05).Conclusion The detection rate of hypertension is lower in elderly patients of Deang nationality than in those of the other nationalities in our country.Economic level,alcohol drinking,BMI,age,gender,divorce or widow,smoking,high salt and fat diet are the major risk factors for hypertension.However,regular eating of vegetables can reduce the risk of hypertension.
4.Cigarette smoke aggravates sertoli/spermatogenic cells damage by activating the ROS/NLRP3 signaling pathway
Shuomin Wang ; Yifan Hu ; Bingxian Zhou ; Shiheng Fan ; Qisheng Wu ; Yayan Fan ; Chunyun Lu ; Dee Yu ; Jing Zhang
Acta Universitatis Medicinalis Anhui 2023;58(9):1557-1564
Objective :
To investigate the mechanism of sertoli/spermatogenic injury induced by cigarette smoke extract(CSE) based on ROS/NLRP3 signaling pathway.
Methods :
The sertoli/spermatogenic of rat were cultured in vitro , and they were divided into control group , cigarette Smoke extract (CSE) group and CSE + NLRP3 inflammosome inhibitor MCC950 group. Cell viability was detected by CCK⁃8 assay; Lactate dehydrogenase ( LDH) was used to detect cell membrane damage ; reactive oxygen species ( ROS) production was used to detect oxidative stress level ; Hoechst/PI fluorescent staining was used to detect cell pyroptosis. Real⁃time quantitative PCR ( RT⁃qPCR) was used to determine the mRNA level of NLRP3 , ASC , Caspase⁃1 , GSDMD , IL⁃1β and IL⁃18. Western blot was used to determine the protein level of NLRP3 , ASC , Caspase⁃1 , GSDMD , IL⁃1β and IL⁃18.
Results:
CCK⁃8 assay showed that cell viability decreased in CSE group (P < 0. 001) , LDH leakage rate (P < 0. 001) and ROS (P < 0. 001) production increased. Compared to the CSE group , cell viability increased (P < 0. 001) , LDH leakage rate (P < 0. 01) and ROS (P < 0. 001) production decreased in CSE + MCC950 group. The number of PI staining cells in CSE group was more than that in control group and CSE + MCC950 group , but staining cells in CSE + MCC950 group was less than that in CSE group. RT⁃qPCR and Western blot results showed that the expression levels of NLRP3 , ASC , Caspase⁃1 , GSDMD , IL⁃1β and IL⁃18 increased in CSE group but decreased in CSE+ MCC950 group.
Conclusion
The oxidative stress and inflammation of sertoli/spermatogenic induced by ciga⁃ rette smoke may be related to the activation of ROS/NLRP3 signaling pathway.
5.Current situation and related factors of occupational stress of employees of a petrochemical enterprise
Qisheng WU ; Bingxian ZHOU ; Shiheng FAN ; Shuomin WANG ; Yifan HU ; Yayan FAN ; Chunyun LU ; De´e YU ; Lei JIN ; Jing ZHANG
Journal of Environmental and Occupational Medicine 2023;40(7):817-822
Background Occupational stress has become one of the main factors affecting people's physical and mental health, and there are many sources of occupational stress in petrochemical enterprises. Objective To evaluate the current situation of occupational stress and its related factors among employees in a petrochemical enterprise, and to provide a scientific basis for reduing the risk of occupational stress among employees in petrochemical enterprises. Methods In June 2022, a cross-sectional questionnaire survey was conducted in a petrochemical enterprise in Hainan, including a general information questionnaire for basic information, the Effort-Reward Imbalance (ERI) for occupational stress, and the Pittsburgh Sleep Quality Index (PSQI) for sleep quality. Chi-square test was used to compare differences in positive occupational stress by demographic characteristics, occupational characteristics, behavior, and occupational disease hazards. Logistic regression was employed to evaluate factors associated with occupational stress. Results Of the