1.The value of P_(300) in judging the true and false coma in patients with closed craniocerebral trauma
Xiangdong ZHAO ; Meilian ZHANG ; Bingling ZHOU
Journal of Clinical Neurology 1992;0(01):-
Objective To study the value of P 300 in judging the true and false coma in patients with closed craniocerebral trauma. Methods The latency and amplitude of abnormal P 300 evoked by visual (image) stimulation were compared and analyzed between 237 cases with closed craniocerebral trauma.which were divided into coma,non coma,false coma and noclear group.Results There was very remarkable diffe rence in the latency and amplitude of P 300 between coma group and non coma group ( P 0 05). There was remarkable difference between the cases whose coma histories were unclear and non coma ( P
2.Expressions of Ki-67, c-myc and p53 in esophageal cancer and their significance
Peigang ZHANG ; Yonghong CHEN ; Gang CHEN ; Bingling MA ; Pirui HE
Cancer Research and Clinic 2014;26(8):544-546,549
Objective To investigate the role of Ki-67,c-myc,p53 in human in esophageal cancer.Methods 25 specimens of human esophageal cancer as observe group and 25 adjacent normal specimens as control group were selected.Expressions of Ki-67,c-myc,p53 protein were detected by immunohistochemical staining.Results Protein expressions levels of Ki-67,c-myc and p53 in human esophageal cancer were significantly higher than those in control group 5.0,2.0,3.0 vs 1.0,0.5,0.3,respectively (all P < 0.05).The expression level of Ki-67 protein in high differentiation group were significantly lower than that of moderate and low differentiation group (3.0 vs 6.0,P < 0.05).In human esophageal cancer specimens,the expression level of Ki-67 protein was positively correlated with that of c-myc (P < 0.05).Conclusion The expressions of Ki-67,c-myc and p53 are higher in esophageal cancer,which are increased with the higher differentiation.Ki-67,c-myc and p53 proteins may be taken as potential markers in human esophageal cancer.
3.Correlation between different scoring indexes of capsule endoscopy and disease activity in Crohn′s disease
Lingyan SHEN ; Juan DU ; Bingling ZHANG ; Chunxiao CHEN
Chinese Journal of Digestion 2015;35(10):654-658
Objective To investigate the correlation between capsule endoscopy Crohn′s disease activity index (CECDAI ) , capsule endoscopy Lewis score , simplified Crohn′s disease activity index (sCDAI) ,C reactive protein (CRP) ,erythrocyte sedimentation rate (ESR) and Crohn′s disease (CD) activity .Methods From April 2009 to April 2014 , 120 patients having received capsule endoscopy examination and diagnosed as CD were collected . The data of CRP and ESR were analyzed . The CD severity was scored by CECDAI ,Lewis score and sCDAI .Spearman correlation was performed to analyze the correlation between CECDAI ,Lewis score ,sCDAI ,CRP and ESR .Results Among all the patients , CECDAI had a good correlation with Lewis score (r=0 .645 , P< 0 .01) and also was correlated with sCDAI (r=0 .190 , P< 0 .05) ,CRP and ESR (r= 0 .315 and 0 .393 ,both P< 0 .01) .However ,the Lewis score was not correlated with sCDAI ,CRP and ESR (r=0 .052 ,0 .041 and 0 .021 ,all P>0 .05) . sCDAI had a good correlation with CRP and ESR (r=0 .438 and 0 .429 ,both P<0 .01) .Among patients whose capsule endoscope failed to pass through the whole small intestinal ,the CECDAI had no significant correlation with sCDAI ,CRP and ESR (r=0 .126 ,0 .181 and 0 .269 ,all P>0 .05) .Conclusions Lewis score ,sCDAI ,CRP and ESR can reflect disease activity of CD patients in a certain degree .CECDAI is the most suitable index in evaluating the disease activity of CD patients .Therefore ,it is necessary to attach importance to capsule endoscopy examination .
4.Preparation of Sustained-Release Tetramethylpyrazine Phosphate Tablet and its Bioavailability on Rabbits
Xilu JIN ; Bingling CHEN ; Weijiang WU ; Zheng GU ; Minghua JIANG ; Zhibo ZHANG ; Jichang DONG
Fudan University Journal of Medical Sciences 2000;27(3):178-180
Purpose To prepare the sustained-release tablet of tetramethylpyrazine phosphate with hydroxypropylmethylcelluose(HPMC) as matrix material. MethodsThe paddle method and the HPLC method were erspectively used determined the cumulative drug released in vitro and the serum concentration in vivo.ResultsThe cumulative drug released in the first hour was about 20%, while in 12 hours it was above 85%. Drug release behavior can be best described by Higuchi equation, and the release rate decreased as the viscosity and/or the amount of HPMC increased. Compared with the market tablet on the rabbits, the sustained release tablet had the decreased peak concentration (P < 0.05 ); the prolonged peak time and mean residence time (P< 0.05).ConclusionsThe matrix tablet was a good sustained-release dosage form and it had a good in vitro-in vivo correlation.
5.Study on the risk factors of measles among the 8-month-olds and children≥15 years of age in Gansu province
Ningjing ZHANG ; Xiaoshu ZHANG ; Hong FU ; Jing AN ; Ying CHEN ; Na JIN ; Bingling ZHANG ; Hui LI
Chinese Journal of Epidemiology 2014;(10):1095-1098
Objective To understand the risk factors of measles among babies under 8-months-old and people ≥15 years old,in Gansu province. Methods Laboratory-confirmed measles cases were divided into two groups among children below 8-months-old and those≥15 year olds. Descriptive epidemiology and 1∶3 case control study were conducted to find out those related risk factors as:hospital exposure,contact with measles cases,vaccination,history of measles etc. so as to determine the risk factors and appropriate control measures. Results Histories of hospital exposure,contact with measles cases and travelling to other cities were risk factors for measles among babies younger than 8-months and people≥15 year olds. Vaccination appeared a protective factor for people ≥15 years of age. From 42 cases and 126 controls,through multivariate analysis,results showed that hospital exposure was the only significant factor(OR=29.23,95%CI:2.82-302.89)for those babies younger than 8-months. Factors as hospital exposure and travelling among cities for 7-21 days before being infected,were with significant importance(OR=5.15,95%CI:2.28-11.63;OR=5.48,95%CI:1.38-21.69)for people ≥15 years of age,according to the observation from 74 cases and 222 controls. Conclusion In order to reduce the incidence of measles among babies under 8-month-old and people ≥15 years old, efforts on control of nosocomial infection should be strengthened,while the routine immunization coverage need to be increased for the children at higher risks,to reduce the source of infection.
6. Primary study on clinical applications of second generation colon capsule endoscopy
Jingjing ZHANG ; Lan LI ; Huajing SU ; Youming LI ; Bingling ZHANG
Chinese Journal of Digestion 2020;40(1):36-39
Objective:
To explore the clinical applications of second generation colon capsule endoscopy (CCE-2).
Methods:
From July 2017 to December 2018, at the First Affiliated Hospital, College of Medicine, Zhejiang University, 40 outpatients and hospitalized patients who underwent CCE-2 examination were enrolled. The examination results were analyzed by an expert gastroenterologist with rich experience in small intestinal and colon capsule endoscopy. The stomach, small bowel and colon transit time, the score of colon cleansing quality, the completion rate of colon capsule examination, lesion detection and adverse effects were observed. Chi-square test and Student′
7.Characterization of thyrotropin receptor molecule and its active peptide as immunogens in BALB/c mice
Yunjuan ZHU ; Ning CHEN ; Lijuan HUANG ; Xiaomei YAO ; Bingling SHEN ; Jing YE ; Yujie DAI ; Xin LIU ; Lijun ZHANG ; Lanying LI ; Fengxian LU
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To study the characterization of thyrotropin receptor (TSHR) and its active fragment TSHR aa352-366 as immunogens in BALB/c mice. METHODS: BALB/c mice were injected peritoneally with TSHR aa352-366-KLH (hemocyanin from keyhole limpets) and the mixture of TSHR aa352-366-KLH and guinea pig TSHR every 15 days, respectively. The levels of thyroid hormones and TSHR antibodies and TSHR mRNA were measured, and the pathological changes of thyroid tissue were observed. RESULTS: In the group injected with TSHR aa352-366-KLH, the serum levels of TT_3 and TT_4 decreased (P
8.Association between SNP rs10569304 on the second expressed region of hole gene and the congenital heart disease.
Yali, ZHANG ; Lin, XU ; Jian, QIU ; Zhiliang, LI ; Linhai, LI ; Guangli, REN ; Airong, DONG ; Bingling, LI ; Mingxiao, GE ; Shiren, MENG ; Jianqing, WANG
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(4):430-6
The correlation of single nucleotide polymorphism (SNP) rs10569304 on the second expressed region of hole gene and congenital heart disease (CHD) of human being, and the effect of hole gene on CHD were investigated. 179 patients with CHD as CHD group and 183 healthy people as control group were selected in the case-control study. DNA was abstracted from the peripheral blood by phenol-chloroform method. Primer was designed for the flanking sequence of SNP rs10569304 on the second expressed region of hole gene. The genotype was identified by PCR degenerative acrylamide electrophoresis with amplification products. Then the three amplification products received sequencing. By chi-square test, the genotype frequency and allele frequency in CHD group and control group were analyzed. There was insertion-deletion (GCC/-) of SNP rs10569304 which corresponded to alleles of A and B in Southern Chinese people. The genotype frequency and allele frequency in control group and CHD group were met the Hardy-Weinberg equilibrium. By chi-square test, in control group and CHD group, the genotype frequency of AA (insertion homozygous), AB (insertion-deletion heterozygous) and BB (deletion homozygous) was 21.31%, 54.09%, 24.59% and 16.75%, 46.36%, 36.87%, respectively. The distributional difference of genotype frequency had statistical significance (chi (2)=6.51, P<0.05); The allele frequency of A and B was 48.36% and 51.64% in control group, 39.94% and 60.06% in CHD group, respectively. The distributional difference of allele frequency had statistical significance (chi (2)=5.20, P<0.05). Meanwhile, by contrast with the control group, the BB genotype frequency and B allele frequency in CHD group was higher, but the AA and AB frequency was lower. There was higher risk to suffer from CHD involving B allele. BB genotype had 1.907-fold increased risk of developing CHD according to AA genotype (P<0.05). It is concluded that there is insertion-deletion (GCC/-) of SNP rs10569304 in the Southern Chinese people, and the people whose hole gene involving BB genotype have higher risk to suffering from CHD.
9.Association between SNP rs10569304 on the Second Expressed Region of Hole Gene and the Congenital Heart Disease
ZHANG YALI ; XU LIN ; QIU JIAN ; LI ZHILIANG ; LI LINHAI ; REN GUANGLI ; DONG AIRONG ; LI BINGLING ; GE MINGXIAO ; MENG SHIREN ; WANG JIANQING
Journal of Huazhong University of Science and Technology (Medical Sciences) 2010;30(4):430-436
The correlation of single nucleotide polymorphism (SNP) rs 10569304 on the second ex-pressed region of hole gene and congenital heart disease (CHD) of human being, and the effect of hole gene on CHD were investigated. 179 patients with CHD as CHD group and 183 healthy people as control group were selected in the case-control study. DNA was abstracted from the peripheral blood by phenol-chloroform method. Primer was designed for the flanking sequence of SNP rs10569304 on the second expressed region of hole gene. The genotype was identified by PCR de-generative acrylamide electrophoresis with amplification products. Then the three amplification products received sequencing. By chi-square test, the genotype frequency and allele frequency in CHD group and control group were analyzed. There was insertion-deletion (GCC/-) of SNP rs10569304 which corresponded to alleles of A and B in Southern Chinese people. The genotype fre-quency and allele frequency in control group and CHD group were met the Hardy-Weinberg equilib-rium. By chi-square test, in control group and CHD group, the genotype frequency of AA (insertion homozygous), AB (insertion-deletion heterozygous) and BB (deletion homozygous) was 21.31%, 54.09%, 24.59% and 16.75%, 46.36%, 36.87%, respectively. The distributional difference of geno-type frequency bad statistical significance (χ2=6.51, P<0.05);The allele frequency of A and B was 48.36% and 51.64% in control group, 39.94% and 60.06% in CHD group, respectively. The distribu-tional difference of allele frequency had statistical significance (χ2=5.20, P<0.05). Meanwhile, by contrast with the control group, the BB genotype frequency and B allele frequency in CHD group was higher, but the AA and AB frequency was lower. There was higher risk to suffer from CHD in-volving B allele. BB genotype had 1.907-fold increased risk of developing CHD according to AA genotype (P<0.05). It is concluded that there is insertion-deletion (GCC/-) of SNP rs10569304 in the Southern Chinese people, and the people whose hole gene involving BB genotype have higher risk to suffering from CHD.
10.Study on the risk factors of measles among the 8-month-olds and children≥15 years of age in Gansu province.
Ningjing ZHANG ; Xiaoshu ZHANG ; Hong FU ; Jing AN ; Ying CHEN ; Na JIN ; Bingling ZHANG ; Hui LI
Chinese Journal of Epidemiology 2014;35(10):1095-1098
OBJECTIVETo understand the risk factors of measles among babies under 8-months-old and people ≥15 years old, in Gansu province.
METHODSLaboratory-confirmed measles cases were divided into two groups among children below 8-months-old and those ≥15 year olds. Descriptive epidemiology and 1:3 case control study were conducted to find out those related risk factors as:hospital exposure, contact with measles cases, vaccination, history of measles etc. so as to determine the risk factors and appropriate control measures.
RESULTSHistories of hospital exposure, contact with measles cases and travelling to other cities were risk factors for measles among babies younger than 8-months and people ≥15 year olds. Vaccination appeared a protective factor for people ≥15 years of age. From 42 cases and 126 controls, through multivariate analysis, results showed that hospital exposure was the only significant factor ( OR = 29.23, 95%CI:2.82-302.89)for those babies younger than 8-months. Factors as hospital exposure and travelling among cities for 7-21 days before being infected, were with significant importance ( OR = 5.15, 95% CI:2.28-11.63; OR = 5.48, 95%CI:1.38-21.69)for people ≥15 years of age, according to the observation from 74 cases and 222 controls.
CONCLUSIONIn order to reduce the incidence of measles among babies under 8-month-old and people ≥15 years old, efforts on control of nosocomial infection should be strengthened, while the routine immunization coverage need to be increased for the children at higher risks, to reduce the source of infection.
Adolescent ; Case-Control Studies ; China ; epidemiology ; Cross Infection ; Humans ; Incidence ; Infant ; Measles ; diagnosis ; epidemiology ; prevention & control ; Measles Vaccine ; administration & dosage ; Risk Factors ; Vaccination ; utilization