Objective To develop a PDA-based wireless electrocardiographic monitoring system meeting the requirements of doctors in mobile work.Methods ECG signals were received from hospital monitor center through mobile network,and then were analyzed with digital signal processing technology and electrocardiographic information processing technology.The results were sent to patients as soon as the data were diagnosed by doctors,thus realizing the real-time monitoring.Results PDA-based wireless electrocardiographic monitoring system applied many advanced technologies such as mobile communication technology,blue-tooth technology,embedded database technology,etc.so that doctors could examine patients' records and electrocardiogram at any place and in any time.Conclusion Clinical experimental results show that the system fulfils doctors' requirements and improves their work greatly.

Objective:To investigate the preventive efficacy of interferon α -2b on scar formation after glaucoma filtering opera-tion. Methods:Totally 62 patients with primary glaucoma(68 eyes)were randomly divided into the observation group(31 cases with 36 eyes)and the control group(31 cases with 32 eyes). The patients in the observation group were given wet compress with a piece of cotton infiltrated by interferonα-2b,while the control group was given wet compress with a piece of cotton infiltrated by mitomycin C. After 4-month postoperative follow-up,the type of filtering bleb,complications and the changes of intraocular pressure were compared between the two groups. Results:The observation group was mainly with type II bleb(58. 33%),while the control group was mainly with type I bleb(53. 13%),and there was significant difference in the filtering bleb type between the two groups(P<0. 05). After the operation,the intraocular pressure at each time point in the two groups was decreased significantly when compared with that before the operation(P<0. 05),while the difference between the two groups was not statistically significant(P>0. 05). The percentage of visual invariableness or improvement in the observation group was significantly higher than that in the control group(P<0. 05),and the occurrence of complications after the operation in the observation group was significantly lower than that in the control group( P<0. 05). Conclusion:Interferon α-2b is an ideal drug for anti-scarring formation after glaucoma filtration surgery.

OBJECTIVETo investigate the effect of RNA interference of IgG gene on the radiosensitivity of the human prostate cancer PC3 cell line.

METHODSPC3 cells were trasnfected via lipofectamine by the shRNA vector FCGR1AshRNA targeting the Fc segment of IgG, using NCshRNA as the negative control. Q-PCR and Western blotting were used to analyze the expression of IgG in the trasnfected cells. The cells were then exposed to ⁶⁰Co γ ray at 0, 2, 4, 6, 8, 10 Gy, and the cell proliferation was evaluated by MTS and the cells apoptosis estimated by flow cytometry at 12, 24 and 48 h.

RESULTSMTS assay showed that ⁶⁰Co γ ray significantly inhibited the proliferation of PC3 cells transfected with FCGR1AshRNA as compared with NCshRNA-transfected and blank control cells (P<0.05). Flow cytometry showed that the cell apoptosis rate was significantly higher in FCGR1AshRNA group than in NCshRNA and blank control groups at 48 h after γ ray exposure (P<0.05). At 12, 24 and 48 h after 6 Gy radiation, the cells in FCGR1AshRNA group showed a significantly lowered proliferation rate and an increased apoptosis rate (P<0.05).

CONCLUSIONThe shRNA targeting IgG gene can significantly enhance the sensitivity of PC3 cells to radiation. The combination of RNA interference targeting IgG gene with radiotherapy may be more effective in the treatment of prostate cancer.

Apoptosis ; Cell Line, Tumor ; radiation effects ; Cell Proliferation ; Humans ; Immunoglobulin G ; genetics ; Male ; Prostatic Neoplasms ; pathology ; RNA Interference ; RNA, Small Interfering ; Radiation Tolerance ; Transfection

Objective To investigate the effect of RNA interference of IgG gene on the radiosensitivity of the human prostate cancer PC3 cell line. Methods PC3 cells were trasnfected via lipofectamine by the shRNA vector FCGR1AshRNA targeting the Fc segment of IgG, using NCshRNA as the negative control. Q-PCR and Western blotting were used to analyze the expression of IgG in the trasnfected cells. The cells were then exposed to 60Co γ ray at 0, 2, 4, 6, 8, 10 Gy, and the cell proliferation was evaluated by MTS and the cells apoptosis estimated by flow cytometry at 12, 24 and 48 h. Results MTS assay showed that 60Coγ ray significantly inhibited the proliferation of PC3 cells transfected with FCGR1AshRNA as compared with NCshRNA-transfected and blank control cells (P<0.05). Flow cytometry showed that the cell apoptosis rate was significantly higher in FCGR1AshRNA group than in NCshRNA and blank control groups at 48 h afterγray exposure (P<0.05). At 12, 24 and 48 h after 6 Gy radiation, the cells in FCGR1AshRNA group showed a significantly lowered proliferation rate and an increased apoptosis rate (P<0.05). Conclusion The shRNA targeting IgG gene can significantly enhance the sensitivity of PC3 cells to radiation. The combination of RNA interference targeting IgG gene with radiotherapy may be more effective in the treatment of prostate cancer.

Objective To investigate the effect of RNA interference of IgG gene on the radiosensitivity of the human prostate cancer PC3 cell line. Methods PC3 cells were trasnfected via lipofectamine by the shRNA vector FCGR1AshRNA targeting the Fc segment of IgG, using NCshRNA as the negative control. Q-PCR and Western blotting were used to analyze the expression of IgG in the trasnfected cells. The cells were then exposed to 60Co γ ray at 0, 2, 4, 6, 8, 10 Gy, and the cell proliferation was evaluated by MTS and the cells apoptosis estimated by flow cytometry at 12, 24 and 48 h. Results MTS assay showed that 60Coγ ray significantly inhibited the proliferation of PC3 cells transfected with FCGR1AshRNA as compared with NCshRNA-transfected and blank control cells (P<0.05). Flow cytometry showed that the cell apoptosis rate was significantly higher in FCGR1AshRNA group than in NCshRNA and blank control groups at 48 h afterγray exposure (P<0.05). At 12, 24 and 48 h after 6 Gy radiation, the cells in FCGR1AshRNA group showed a significantly lowered proliferation rate and an increased apoptosis rate (P<0.05). Conclusion The shRNA targeting IgG gene can significantly enhance the sensitivity of PC3 cells to radiation. The combination of RNA interference targeting IgG gene with radiotherapy may be more effective in the treatment of prostate cancer.

with hyperbilirubinemia admitted to the Neonatology Department of the First Affiliated Hospital of Zhengzhou University from January 2016 to October 2018 were divided into the control group (n=51) and the observation group (n=51) by random number table method. Both groups underwent auditory brainstem response hearing screening. The control group was given routine nursing intervention, the observation group was given non-drug sedation nursing intervention. The sedative effects of the two groups were compared. The Score of Newborn Facial Coding System (NFCS) and effective rates of examination between the two groups were compared. Results? After intervention, mean heart rate and mean respiratory rate in the observation group were statistically lower than those in the control group (P＜0.05), and average sleep latency was significantly shorter than that in the control group with statistical difference(P＜0.05). After intervention, only NFCS score in the control group significantly increased compared with that before intervention, and the NFCS score of the observation group was significantly lower than that of the control group during and after screening with statistical difference (P＜0.05). The effective rate of examination in the observation group was 98.04%, significantly higher than that of the control group (86.27%) with statistical difference(χ2=4.883,P＜0.05). Conclusions? The application of non-drug sedative nursing intervention in auditory brainstem response in hyperbilirubinemia neonatal hearing screening has good sedative effect, can significantly reduce the stress response of children to the outside world, and improve the efficiency of examination. It has certain application value in neonatal medical examination.

OBJECTIVETo study the expression of OCT4 protein in bladder cancer and its correlation to the clinicopathologic features and prognosis of bladder cancer.

METHODSOCT4 mRNA and protein expression was detected in 5 bladder cancer cell lines (RT-4, Tcc-Sup, KK47, T24, and 5637) and 1 normal bladder cell lines by real-time PCR and Western blotting, respectively. Immunohistochemical analysis was used to detect the expression of OCT4 protein in 46 bladder cancer samples.

RESULTSAll the 5 bladder cancer cell lines expressed detectable levels of OCT4 mRNA and proteins, whereas the normal bladder cell line SV-HUC-1 was negative for OCT4 expression. The clinical bladder cancer tissues showed a high positivity rate of OCT4 expression (76.1%), which was not detected in normal bladder tissues. Specific OCT-4 signals were localized mainly in the nuclei of the cancer cells. The expression rate of OCT4 protein was significantly higher in bladder cancer tissue than in normal bladder epithelium (P<0.05), and showed a positive correlation to the grade of tumor differentiation and metastasis (P<0.05) but not to the patients' age, gender or TNM stage.

CONCLUSIONOCT4 protein expression is associated with tumor differentiation and metastasis in bladder cancer and may play an important role in the early diagnosis and prognostic evaluation of bladder cancer.

Cell Line, Tumor ; Humans ; Neoplasm Metastasis ; Neoplasm Staging ; Octamer Transcription Factor-3 ; metabolism ; Prognosis ; Urinary Bladder Neoplasms ; diagnosis ; metabolism ; pathology