Objective:To explore the effect of diabetes specific Enteral Nutritional Suspension on patients with severe brain injury.Methods:from March 2018 to April 2021, 117 patients with severe brain injury were selected from the Department of Neurosurgery of Yantai Mountain hospital of Yantai city. They were divided into 2 groups according to the random number table. The control group received enteral nutrition (EN) preparation (n=59) , while the observation group was treated with diabetes specific Enteral Nutritional Suspension (n=58) for 2 weeks, and two groups of immunoglobulin A were compared. (IGA) , immunoglobulin G (IgG) , immunoglobulin M (IgM) , total cholesterol (TC) , low density lipoprotein (LDL) , triglyceride (TG) , glycosylated hemoglobin (HBAIC) , fasting blood glucose (FBG) , 2 h postprandial blood glucose (2hbg) and the positive rate of bacterial DNA in peripheral blood at each time period. The positive rate was analyzed by SPSS 22.0 software χ 2 test, repeated measurement was analyzed by analysis of variance, and pairwise comparison was performed by LSD-t test.Results:The positive rates of bacterial DNA in peripheral blood of the observation group were 10.34%, 5.17% and 0.00% on the 7th day and 14th day after treatment, which were lower than 25.42%, 16.95% and 8.47% in the control group (P = 0.034, 0.043 and 0.023) . 2 weeks after treatment, the levels of BG (7.46±1.22) mmol/L, FBG (5.26±1.11) mmol/L, HBAIC (6.33±0.45%) , TG (1.11±0.12) mmol/L, TC (4.22±0.68) mmol/L, LDL (1.39±0.13) mmol/L in the observation group were lower than those in the control group, 2hbg (10.69±1.57) mmol/L, FBG (8.18±1.46) mmol/L, HBAIC (7.46±0.34%) , TG (1.86±0.26) mmol/L, TC (6.65±1.17) mmol/L and LDL (2.79±0.41) mmol/L, ( P<0.001) , IgM, IgA and IgG were (1.57±0.26) g/L, (1.86±0.43) g/L, (10.13±1.46) g/L, and IgM, IgA and IgG were (1.86±0.47) g/L, (2.86±0.39) g/L, (13.28±1.96) g/L, respectively, 1 week after treatment. The improvement of all indexes were better than that of the control group The IgM, IgA and IgG of (0.86±0.13) g/L, (1.35±0.11) g/L, (8.66±1.57) g/L and 2 weeks after treatment were (1.10±0.11) g/L, (2.13±0.11) g/L and (10.45±1.46) g/L respectively ( P<0.001) . Conclusion:The special enteral nutrition suspension for diabetes patients with severe brain injury can improve the immune function and glucose and lipid metabolism of the body, and reduce the positive rate of bacterial DNA.