Objective:To investigate the effect of mannose on the radiosensitivity of six human non-small cell lung cancer cell lines and its possible mechanism.Methods:The expression of mannose phosphate isomerase in six lung cancer cell lines were detected by Western blot. The inhibitory effect of mannose on the proliferation of lung cancer cell lines were observed by MTT assay. When irradiated with 0, 2, 4, 6, 8 and 10 Gy, the effect of mannose on the radiosensitivity of six lung cancer cell lines was detected by plate clone formation assay, respectively; and the apoptosis rates of normal control, mannose, irradiation and combined groups were detected by flow cytometry.Results:The expression levels of mannose phosphate isomerase were different among six lung cancer cell lines. Among them, A549 cells had the highest expression level and H460 cells showed the lowest expression level. When aD ministrated with 11.1 mmol/L mannose, the same inhibitory effect was observed on both A549 and H460 cell lines. Moreover, the inhibitory effect on H460 cell line was significantly increased with the increase of mannose concentration. In addition, aD ministration of 11.1 mmol/L mannose could significantly increase the radiosensitivity and apoptosis rate of H460 cell line. However, it exerted limited effect upon the radiosensitivity and apoptosis rate of A549 cell line. Conclusion:In six lung cancer cell lines with high expression of mannose phosphate isomerase, the aD ministration of mannose can enhance the radiosensitivity of partial tumors cells.

Objective Cardiac arrest is a major cause of death worldwide.Hypothermia is an important protective method against global cerebral ischemia (GI) following cardiac arrest.However,the mechanisms of hypothermia underlying the changes in different regions and nodes of the brain have not been fully elucidated.Methods In this study,We investigated the metabolism of brain and its nodes in rat hypothermia GI and normothermia GI models using positron emission tomography (PET) and magnetic resonance imaging (MRI).Voxel-based analysis was used to assess the metabolic nodes.To identify the cerebral circuit of potential hypothermia protection,a white matter network of hypothermia GI and normothermia GI was constructed using fractional anisotropy from diffusion tensor imaging (DTI) and the nuclei on the pathway were also identified.Results The PET voxel-based analysis demonstrated that there was significant reduce hypometabolic region of the brain in the hypothermia GI group compared with that in the normothermia GI group.Hypothermia selectively preserved the mesocircuit including the thalamus,prefrontal cortex,and the connections between them,including those between the corpus callosum and internal capsule.Conclusion In rats that underwent hypothermia,the preserved functional integrity of the anterior forebrain mesocircuit.We provide evidence that the anterior forebrain mesocircuit is relatively preserved in hypothermia and has an important role in cerebral protection.