1.Molecular epidemiological study on human metapneumovirus infection among children in Guangzhou
Jiayu ZHONG ; Bing ZHUB ; Li DENG ; Mingxiu ZHOU ; Yi CHEN ; Jiahui XIE ; Yingying ZHANG ; Lu KUANG ; Misi XIAO
Chinese Journal of Clinical Infectious Diseases 2011;04(4):223-226
ObjectiveTo conduct a molecular epidemiological study on human metapneumovirus (hMPV) among pediatric patients in Guangzhou. MethodsA total of 1 840 clinical specimens were obtained from pediatric patients with respiratory infections in Guangzhou Women and Children' s Medical Center in 2010.hMPV was detected by real-time TaqMan RT-PCR in clinical specimens.F gene was amplified and the PCR-products were directly sequenced. ResultsIn 1 840 clinical specimens, 66 werehMPV-positive with a positive rate of 3.59%. hMPV was detected in all specimens except those collected in September and October, and the highest positive hMPV rate occurred in April (6.09%). The F genes of 3 randomly selected strains and hMPVgz01 ( isolated in 2008) were compared with subgroups A1, A2, B1,B2 and C, and the highest homology was with BJ1887 strain of genotype A2b (97%). The F genes of the randomly selected strains and hMPVgz01 were 99% identical to each other. Sequences and phylogenetics analysis revealed that the epidemical strain in Guangzhou belonged to genotype A2b. ConclusionhMPV is prevalent in spring and summer among children in Guangzhou, and A2b is the predominant genotype.