Kidney theory is an important figure in traditional Chinese medicine (TCM), it proposes that the kidney is the root of life, in which stores congenital essence and energy of human body that promotes reproduction, growth and development. The facts that body development begins from oosperm have long been elucidated in modern medicine and life science. Furthermore, oosperm is a totipotent stem cell. The totipotent stem cell with its derived embryonic stem cell (ESC) and stem cell constitute the source of growth, development and tissue regeneration. It is rational to comprehend ESC by means of the Kidney theory of TCM, investigate the effect of Kidney-nourishing on the activities of ESC, such as proliferation, aging and apoptosis, and on its related genes regarding transcription, expression and functional regulation. Our works have suggested that classical Kidney-yin-nourishing herb formula Zuo-gui-wan could inhibit the ESC differentiation and apoptosis, stimulate proliferation, and promote the process of cell cycle. These observations coincide with up-regulate expression of Wnt and Oct3/4, and down-regulate expression of P16INK4a. At the same time, classical Kidney-yang-nourishing herb formula You-gui-wan could inhibit ESC apoptosis induced by H2O2, up-regulate Notch expression and down-regulate P16INK4a expression. In addition, there have been some evidences indicated Kidney-nourishing could promote mesenchymal stem cells proliferate. These observations strongly suggest that, Kidney theory of TCM, as the root of the life, is associated with ESC and stem cell; it may provide insight into Kidney theory of TCM and stem cell research.

Objective To evaluate the comprehensive experiment in fundamental nursing by using the indicator system and analyze the teaching effect.Methods 450 nursing graduates of grade 2011 were divided into 56 groups and comprehensive experiments were graded by using the indicator system in each group.Results The four indexes in level one scored (18.08±1.50),(19.99±2.31),(28.18±3.35),(18.16±1.73) points and accounted for 90％ 、80％ 、81％、91％ respectively.The total points' scorings and proportions were (84.41±5.84) points and 84％ respectively.Some deficiencies were found to be improved during evaluation.Conclusions Nursing comprehensive experiment evaluation indicator system can assess comprehensive experiments well,and the related competencies of nursing graduates need to be improved.

Cell senescence is an important anti-cancer mechanism and may contribute to cancer therapeutic outcome. The present study observed the effects of Tenglong Buzhong Decoction (TLBZD), a Chinese herbal formula, on senescence in human colon carcinoma LS-174-T cells.

Objective: To observe the effects of Tenglong Buzhong Decoction (TLBZD), a compound traditional Chinese herbal medicine, on proliferation and apoptosis of colon carcinoma cell line LS174T in vitro. Methods: Human colon carcinoma cell line LS174T and human colon epithelial cell line CRL-1790 were treated with different doses of TLBZD. Cell proliferation was detected with cell counting kit-8 (CCK-8) assay and clone formation assay. Cell cycle and apoptosis were detected by flow cytometry, and caspase-3, -8 and -9 activities in LS174T cells were detected by colorimetric assay. Results: TLBZD had no obvious cytotoxicity in normal CRL-1790 cells. After 72-hour treatment of 1 mg/mL TLBZD, or 48- and 72-hour of 2 mg/mL TLBZD, or 24-, 48- and 72-hour of 5-20 mg/mL TLBZD, proliferation of LS174T cells was significantly inhibited. Clone formation of LS174T cells was significantly inhibited by 1 to 20 mg/mL TLBZD treatment. TLBZD at doses of 5 to 20 mg/mL also induced apoptosis and cell cycle arrest at G(0)/G(1) phase in LS174T cells. In addition, caspase-3, -8 and -9 activities were significantly elevated after 5 to 20 mg/mL TLBZD treatment. Conclusion: TLBZD can inhibit cell proliferation, arrest cell cycle at G(0)/G(1) phase, and induce apoptosis in LS174T cells, which may be related to activating of caspase-3, -8 and -9.

HPV DNA were detected in 60 biopsies from women with condyloma acuminata (vulva 47,Vagina 12,cervix 1)by dot blot hybridiztion;physical states of 8 HPV 11 DNA were analyzed by techniques of restriction endonuclease and Southern blot hybridization. HPV 11 was found in 19biopsies of vulva (13 ). vagina (5)and cervix (1),and HPV 16 and 18 in lvaginal and 1 vulvar biopsy respectively. All of HPV 11 DNA were episomal in cells . Our results provided the data for further studying the relationship between HPV and genital cancer in our country.

Objective To observe the short-term curative effect of biventricular cardiac resynchronization therapy in patients with chronic heart failure.Methods Twenty-six patients with chronic heart failure underwent biventricular cardiac resynchronization therapy,and a left ventricle lead was implanted from the coronary sinus to vein.During the 3-34(13.8?10.4)months of follow-up period,the following parameters were recorded:the cardiac function,patients' activity,heart rate at night,QRS wave width,left ventricular end-systolic dimension(LVESD),left ventricular end-diastolic dimension(LVEDD),end-systolic volume(ESV),end-diastolic volume(EDV),left ventricular ejection fraction(LVEF),left ventricular filling time,and standard deviation of time to regional peak systolic velocity(Ts-Sd-12).Results One out of the overall 26 patients died from sudden cardiac death.The cardiac function was improved in the 25 survivals in the 3-months follow-up,showing that the effective rate of CRT was 96.2%.For the 25 patients,the cardiac function was improved according to New York Heart Association(NYHA)functional class from Ⅲ-Ⅳ grade to Ⅱ-Ⅲ grade.The average total activity time was increased from 0.31?0.40 hours per day to 2.35?1.20 hours per day.Average heart rate during night-time was decreased from 84.8?15.7 beats per minute to 63.4?4.5 beats per minute,variability of cardiac rhythm was raised from 47.3?7.1ms to 96.4?15.1ms;QRS duration was shortened from 158.6?31.8ms to 129.5?30.2ms(P

Objective To investigate the relationship between the different deep venous drainage patterns in the brain and the perimesencephalic subarachnoid hemorrhage (PMSAH).Methods From January 2014 to January 2017,the clinical data of 90 patients with subarachnoid hemorrhage (SAH) diagnosed and treated in the Second Affiliated Hospital of Anhui Medical University were analyzed retrospectively.Thirty patients with PMSAH were in a PMSAH group and 60 patients with aneurismal SAH were in a control group.Unilateral cerebral hemisphere venous drainage was divided into type A (normal continuous):the basilar vein had deep middle cerebral vein drainage and was drained into the great cerebral vein of Galen;type B (normal discontinuous):there was discontinuous venous drainage between the basal vein and the anterior uncal vein and the posterior Galen vein;type C (primitive variant):did not drained into great cerebral vein of Galen,perimesencephalic vein was drained into the superior petrosal sinus or basal vein was directly drained into the transverse sinus or straight sinus.The different combinations of bilateral cerebral hemisphere venous drainage were divided into normal type drainage (typeⅠ:AA),discontinuous type drainage (types Ⅱ:AB or BB),and primitive type drainage (types Ⅲ:AC,BC,or CC).The differences of venous drainage between the two groups were compared.Results In the PMSAH group,both types Ⅰ and Ⅱ drainages accounted for 26.7％ (n=8 in each type) and type Ⅲ accounted for 46.7％ (n=14).In the control group,typeⅠaccounted for 48.3％ (n=29),type Ⅱ accounted for 28.3％ (n=17),and type Ⅲ accounted for 23.3％ (n=14).There were no significant differences in the distribution of three venous drainage patterns between the two groups (χ2=5.804,P=0.055).However,there was significant difference in the types Ⅲ venous drainage between the two groups (χ2=5.081,P=0.024).Conclusion Most of the deep cerebral venous drainage in patients with PMSAH showed basilar venous drainage into the venous sinuses of dura mater,but not to the large cerebral vein drainage,suggesting the way of primitive drainage into the dural sinus was more prone to rupture compared with that of drainage into large cerebral veins.

Cyber -knife a new stereotaxic radiosurgery instrument, which structure and technical characteristics are introduced. By application of real-time image aiming and synchronous dynamic tracking techniques, abandoning vicious location frame to solve the questions of traditional directional radiosurgery technique only use pate tumour. Cyber-knife is an innovative stereotaxic radiosurgery instrument, extends the indications of stereotaxic radiosurgery from intracranial neoplasms to extracranial tumors and has a widest development prospect in medical treatment.

Objective To investigate the expression of costimulatory molecules CD80and CD86on peripheral blood lymphocytes(PBLC)in patients with systemic lupus erythematosus(SLE)and its significance.Methods The expression of CD80and CD86on PBLC was measured by flow cytometric assay in30patients with SLE and25normal controls.Results Expression of CD86on PBLC in patients with SLE was significantly lower than that of normal controls(P