Fall has seriously impaired the quality of life of old people. The main risk factors for fall in the old people are concerned with balance dysfunction which is caused by ageing. Taiji Quan exercise can promote the lower limb muscle strength, proprioception, neuro-muscular reaction, gait and cognition of the old people, that plays a role in fall prevention.

PCI-32765,an oral selective and irreversible inhibitor of Bruton tyrosine kinase (BTK),inhibits survival,activation,proliferation and migration of malignant B cells by blocking B cell receptor signaling pathway.PCI-32765 not only acts on malignant B cell,but also prevents resistance to chemical drugs.Therefore,PCI-32765 has broad prospects in the treatment of B cell malignancies.

Objective To investigate the association between SERPINH1 gene expression in gastric cancer tissue with the pathology and prognosis and its action mechanism in gastric cancer.Methods The gastric cancer data in the TCGA and GEO public databases were collected,and the retrospective analysis and survival analysis were conducted on the SERPINH1 expression data of the tumor tissue samples and related clinical information;the gene set enrichment analysis(GSEA) was used to analyze the SERPINH1 expression related functional gene sets and to investigate the SERPINH1 action mechanism.Results In 421 cases of gastric cancer sample,the SERPINH1 expression level had no significant differences with the tumor grade,N stage and metastasis.However,its high expression was significantly correlated with the tumor invasion depth(T stage)(P=0.049).The higher the expression level,the preater the invasion depth.In the survival analysis,the patients with high SERPINH1 expression showed a poorer prognosis than those with low SERPINH1 expression[P<0.001,HR(95%CI):1.97 (1.61-2.41)].The gene set enrichment analaysis found that the related gene sets such as tumor signal pathway and tumor microenvironment were enriched in the SERPINH1 high expression samples.The gene expression chip data in 69 cases of gastric cancer and paracancerous tissue also suggested that SERPINH1 was highly expressed in gastric cancer (P<0.000 1) with a greater diagnostic value (AUC=0.988 1).Conclusion High expression of SERPINH1 is correlated with the occurrence and development of gastric cancer,and has a greater clinical significance.

OBJECTIVE:To establish a method for the rapid identification of Xiangsha pingwei pill(watered pill)from different manufacturers. METHODS:Near-infared spectroscopic method was conducted to collect the infrared spectroscopy of 95 batches of Xiangsha pingwei pill(watered pill) from 5 manufacturers,first derivative was combined with vector normalization to analyze the spectral data,mathematical models were established and validated. RESULTS:When the CI value was 5.6,the established model can identify samples from Li Shizhen Pharmaceutical Group Co.,Ltd. quickly,the model was validated to be achievable;and when the CI value was 4.7,the established model can identify Samples from Jinma Pharmaceutical Co.,Ltd. in Shangqiu city quickly, the model was validated to be achievable. CONCLUSIONS:The method is convenient,rapid and non-destructive,and can identify different Xiangsha pingwei pill(watered pill)from different manufacturers quickly.

OBJECTIVE: To observe the effects of different concentrations of arsenic trioxide (As(2)O(3)) on apoptosis and proliferation of human lung cancer cell line A549 in vitro under hypoxia and normoxia. METHODS: A549 cells were treated with 0, 1, 2, 4 micromol/L As2O3 for 12, 24 and 48 h under hypoxia (5% O(2)) and normoxia (21% O(2)). The proliferative inhibition rate of A549 cells was measured with methyl thiazolyl tetrazolium assay, and the apoptotic rate of A549 cells was detected by Annexin V/propidium iodide (PI) double staining. RESULTS: Under normoxia and hypoxia, 1, 2, 4 micromol/L As(2)O(3) could significantly inhibit the proliferation of A549 cells and induce the apoptosis of A549 cells. The results depended on the drug concentration and action time. And the hypoxia couldn't influence the effects of As(2)O(3). CONCLUSION: As(2)O(3) can inhibit the proliferation and induce the apoptosis of A549 cells under hypoxia and normoxia.

Objective To analyze left ventricular(LV) and right ventricular(RV) myocardial function in fetuses,quantitative tissue velocity imaging (QTVI) was used to detect myocardial performance index (Tei index).Methods The study group consisted of 77 second and third trimester conceived normal fetuses.The isovolumic relaxation time (IRT) + isovolumic contraction time (ICT) and ejection time (ET) of LV,RV and internal ventricular septum were obtained by QTVI.Tei indexes were calculated using the formula (IRT+ICT)/ET.Results The fetal IRT+ICT,ET and Tei index can be easily obtained in all fetuses.There was no significant difference for LV and RV Tei index (LV: 0.49 ? 0.10 ;RV: 0.50 ? 0.13 ).The IRT + ICT and ET were inversely proportional to fetal heart rate.The Tei index,however,appeared to be independent of fetal heart rate and of gestation age.Conclusions QTVI provides a new,easily obtainable and reproducible measure to assess fetal ventricular function.

BACKGROUND: Some researches demonstrate that tea polyphenols (TP) has protective effects on neurotoxicity of hippocampal nerve cells induced byβ-amyloid peptide (Aβ), 6-hydroxydopamine (6-OHDA) and oxidative substances. In addition, clinical preliminary examination indicates that TP plays a certain preventive and therapeutic effects on the reduction of recognition function in high-risk population with Alzheimer disease (AD); however, its target and mechanism are still hot topics.OBJECTIVE: To observe the interfering effects of TP on cerebral nerve cell apoptosis induced by D-galactose and Aβ25～35 in mice.DESIGN: Randomized controlled animal study.SETTING: Department of Pharmacology, Pharmacological College of Jinan University.MATERIALS: The experiment was carried out in the Experimental Center of Jinan University from September 2004 to January 2005. A total of 90 healthy Kumning mice, aged 2 months, each gender in half, weighing 26-28 g, were provided by Guangdong Provincial Medical Laboratory Animal Center. Tea polyphenols was provided by Zhejiang Oriental Tea Science and Technology Corporation (batch number: 20040203); D-galactose by Shanghai Number 2 Reagent Plant (batch number: 20030708); Aβ25～35 by Sigma (batch number: 13/01/2004); vitamin E (Vit-E) by Shanghai Xinyi Pharmaceutical Factory (batch number: 20030708).METHODS: Experimental interference: Mice based on body mass were randomly divided into 6 groups: sham operation group (n =17), model group (n =16), vitamin E group (n =16), low-dose (n =13), moderate-dose (n =14) and high-dose (n =14) tea polyphenols groups. In above-mentioned animals, except those in the sham operation group, all were given 120 mg/(kg·d) D-galactose for 12 consecutive weeks, and Aβ25～35 (4 nmol) was slowly injected into the lateral cerebral ventricle. In sham operation group, the same volume of artificial cerebral spinal fluid (CSF) was internally injected into lateral ventricle. Drug treatment began at the first week. Mice in the sham operation group and model group were given distilled water, and the animals in other groups were given the above-mentioned drugs (100 mg/kg Vit-E, 100, 250 and 625 mg/kg TP), respectively. The volume of perfusion was 10 ml/kg, and the treatment lasted for 12 consecutive weeks. Experimental evaluation: After administration, LW-Ⅱ water maze was used to measure learning and memory condition; brain, liver tissues and serum were obtained to measure activity of superoxide dismutase (SOD) and content of malondialdehyde (MDA); Fura-2/AM loading method was used to measure Ca2+ concentration in erythrocytes and neurons; flow cytometer was used to detect cerebral nerve cell apoptosis.MAIN OUTCOME MEASURES: Cl) Learning and memory ability; (2) SOD activity and MDA content in serum, liver and brain tissues; (3) Ca2+ concentration in erythrocytes and neurons;flow cytometer was used to cerebral nerve cell apoptosis.MAIN OUTCOME MEASURES：①Learning and memory ability;②SOD activity and MDA content in serum,liver and brain tissues；③Ca2+ concentration in erythrocytes and neurons；④cerebral nerve cell apoptosis．RESULTS：All 90 mice were involved in the final analysis．①At 12 weeks after administration,time to swim out of the water maze in the moderete-dose and high-dose TP groups and Vit-E group was shorter than that in the model group,and numbers of errors in passing the blind alleys in the water maze was reduced as compared with those in the model group,and there was significant difference(P＜0．05-0．01)．②SOD activities in the moderate-dose and high-dose TP groups were increased as compared with that in the model group,but MDA content in the high-dose TP group was decreased as compared with that in the model group．There was significant difference(P＜0．05-0．01)．③Ca2+ concentration in erythrocytes and neurons in the modemte-dose and high-dose TP groups and Vit-E group was lower than that in the model group,and there was significant difference(P＜0．05-0．01)．④The rates of brain neurons apoptosis in treatment groups with different doses of TP were 12．6％，18．6％,and 24．1％ respectively, exhibiting significant difference as compared with the mice in sham operation group(P＜0．05-0．01) CONCLUSION：TP can inhibit cerebral nerve cell apoptosis induced by D-galactose and Aβ25～35 and improve learning and memory ability in model mice．The effects may be related to its action of raising general anti-oxidative ability and improvement of intrecellular Ca overload induced by oxidative stress injury．

Objective:To investigate the effects of maternal malnutrition on the expression of the insulin signal transduction proteins in the liver of perinatal rats.Methods:(1)The dams were semi-starvation from the first day during pregnancy to build the animal model of intrauterine growth retardation(IUGR).On days 15,17,19,21(E15,E17,E19,E21)of gestation,the fetuses were delivered by cesarean section.The fetal rats,placenta,fetal liver and the fetal brain were weighed respectively.And the placenta weight to the body weight ratio(PWR),the liver weight to the body weight ratio(LWR),the brain weight to the body weight ratio(BWR)were calculated;The expressions of the hepatic insulin receptor(IR),insulin receptor substrate-1(IRS-1),insulin receptor subtrate-2(IRS-2),phosphatidyl inositol 3-kinse(PI3K)mRNA in the rats during perinatal period were detected by RT-PCR.Results:(1)The PWR of the IUGR fetus on E15 was lower than that in the control(P0.05).Conclusion:(1)In order to protect the development of brain,the fetus that suffered maternal malnutrition throughout the pregnancy had liver weight and body weight reduced much more than brain weight,which was known as "brain spare effects".(2)The expressions of hepatic IR mRNA and PI3K mRNA of the fetus were in normal ranges;But the expressions of the hepatic IRS-1 mRNA and IRS-2 mRNA of the IUGR fetus were decreased during the perinatal period.These may be related to the growth retardation and insulin resistance of the adult IUGR rats.

Objective:To investigate the expressions of the insulin receptor(IR),insulin receptor substrate-1(IRS-1),insulin receptor substrate-2(IRS-2),phosphatidyl inositol 3-kinase(PI3K) and insulin-like growth factor-1(IGF-1) of the livers of the male adult rats born with intrauterine growth retardation(IUGR),and to find out the relationship between IUGR and insulin resistance in their adult life.Methods:The foods were available ad libitum throughout the pregnancy to the control group and the rats in the experimental group were fed 50% of the control group to build the IUGR animal model.Liver samples were collected when the male offspring grew up to 12 weeks old.The mRNA expressions of hepatic IR,IRS-1,IRS-2,PI3K,and IGF-1 were detected by RT-PCR.The protein expressions of hepatic IR,IRS-1,and IRS-2 were analyzed by immunohistochemistry.Results:The mRNA expressions of hepatic IR in the adult male rats with IUGR were significantly lower than control group[(0.41?0.06) vs(0.62?0.11),P0.05] in both groups.The mRNA and protein expressions of the hepatic IRS-1[mRNA:(0.77?0.20) vs(1.32?0.42),P0.05].The expressions of hepatic IGF-1 mRNA of the rats with IUGR were significantly lower than those in control group [(0.55?0.12) vs(1.22?0.34),P

Objective To analyze the CRE and UREA result of university students,and establish the refer-ence interval for CRE,UREA adapted to this university.Methods Performing serum analysis of 2 926 freshmen for CRE,UREA with the automatic biochemical analyzer ( Olympus AU400 ) .Results Reference intervals were get among freshmen as CRE:74.00-109.00μmol/L (M),59.00-85.00 μmol/L (F),and it was different between male and female(Z =-27.27,P <0.01);UREA:2.70 -6.70mmol/L (male aged 16 -24 years),1.90 -5.70mmol/L (female aged 16-24 years),2.30-6.57mmol/L (male aged >24-45 years),1.94-5.11mmol/L ( female aged 25-45 years) .Conclusion The reference intervals disaggregated by sex and age are initially estab-lished.