1.Effect of Extracts of Herba(RPMP) on the Growth and Proliferation of Cultured Melanocytes
Kuanhou MU ; Xianqi ZHANG ; Bing YU
Journal of Chinese Physician 2000;0(11):-
Objective To study of the effect of traditional Chinese medicine(TCM): Herba radix polygoni multiflori preparata (RPMP) on the cultured melanocytes(MC). Methods Extracts of TMC RPMP treated the cultured melanocytes, and tyrosinase activity, melanin level and proliferation of the cells were determined. Meanwhile, method of micropore filter was used to measure the ability of cell migration. Results TCM RPMP markedly promoted the proliferation and migration of MC in a dose-dependent manner at a range of 0~150?g/ml. Conclusions TCM RPMP can promote the proliferation and migration of MC in vitro. And it suggests that this drug could be used to treat vitiligo.
2.Clinical study of the emodin thermosensitive hydrogel in the treatment of chronic periodontitis
Bing ZHAO ; Quan ZHAO ; Wei PENG ; Yu MU
Chinese Journal of Primary Medicine and Pharmacy 2010;17(16):2165-2167
Objective To evaluate the clinical effects of scaling and root planning combined with application of the emodin thermosensitive hydrogel on periodontitis. Methods Forty patients with chronic periodontitis with a total of eighty teeth were included in the study.For each patient after scaling and root planning,the teeth on one side were assigned as the test group and treated with the emodin thermosensitive hydrogel in the pocket once a week for four weeks.The teeth on the other side were assigned as the control group.All the clinical parameters were recorded at the baseline,the 6th week and 12th week after the treatment. Results At the baseline,there was no difference in the clinical parameters between the test group and the control group(P>0.05).A statistically significant improvement of all clinical parameters was observed in both groups 6w later(t = 39.06、62.76、20.50、8.05、158.32、31.45、46.53、8.43、8.08、8.03)(P<0.01=,and all clinical parameters of the test group were better than those of the control group,the differences were statistically significant(t = 31.50、27.91、3.92、7.38、20.09)(P<0.01=. Conclusion The emodin thermosensitive hydrogel could effectively control the symptoms of periodontitis.
3.High-dose methotrexate plus hematopoietic stem cell transplantation supplemented by rituximab intrathecal injection for primary central nervous system lymphoma:two cases report and literature review.
Jin-hong JIANG ; Bing-mu FANG ; Ye-hui XU
Chinese Journal of Hematology 2013;34(2):162-163
Adult
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Antibodies, Monoclonal, Murine-Derived
;
therapeutic use
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Central Nervous System Neoplasms
;
therapy
;
Female
;
Hematopoietic Stem Cell Transplantation
;
Humans
;
Injections, Spinal
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Lymphoma, Non-Hodgkin
;
therapy
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Male
;
Methotrexate
;
administration & dosage
;
therapeutic use
;
Middle Aged
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Rituximab
4.Study of hibernating myocardium with high altitude heart disease.
Xian-bo PENG ; Xin-bing MU ; Feng HE
Chinese Journal of Applied Physiology 2003;19(2):148-149
Adult
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Aged
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Altitude
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Echocardiography, Stress
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Female
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Heart Failure
;
diagnostic imaging
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physiopathology
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Humans
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Male
;
Middle Aged
;
Young Adult
5.In vitro differentiation of islet-like cells from mouse bone-marrow stem cells on a PLGA scaffold
Yimin SONG ; Chen LIN ; Xiaobin LI ; Bing MU ; Yupei ZHAO
International Journal of Biomedical Engineering 2014;37(5):-
Objective To investigated the potential influences of poly(lactic-co-glycolicacid)(PLGA)scaffold as a platform on the differentiation of mouse bone-marrow stem cells to islet-like cells.Methods Mouse bonemarrow stem cells were grown and differentiated in culture with or without PLGA scaffold,and cell morphology and functions were compared within these groups.Results The PLGA scaffold showed fine biological compatibility.Differentiated islet-like cells were dithizone (DTZ) positive,insulin and C-peptide double positive,glucagon positive and somatostatin positive in both groups.Under electron microscope there were ultrastructures similar to that of islet β cells in cells of both groups.Cells with PLGA scaffold secreted more insulin under high level glucose stimulation (P<0.01).Conclusions PLGA scaffold was biologically compatible and improved function of the differentiated islet-like cells.
6.Management and prognosis of urothra calculi(Report of 46 cases)
Shu CUI ; Jiankuen YANG ; Mu ALI ; Bing MA ; Lati MU ; Xinfu SHENG
Journal of Clinical Urology 2001;16(6):263-264
Purpose:To investigate the management and prognosis of urethral calcali.Methods:46 case of urethral calculi have been studied and followed up, 45 cases are males, 1 case is female. Mean age of the patients was 41 years. All patients complained of dysuria and urinary pains, acute retention was present in 10 cases. 3 cases have haematuria. Associated urethral disease was found in 12 patients.Results:The 37 cases were treated by endoscopic manipulation of choice for urethral calculi and have been followed up and have been all recovered except only 1 case.Conclusions:Endoscopic manipulation was found to be the safest and the most effective procedure.
7.Discussion on the impact of acupuncture for the pregnancy of in vitro fertilization-embryo transfer.
Zhengyun XIE ; Bing YAO ; Li CHEN ; Yanyun MU ; Jie CHENG ; Qian LI ; Zhao GAO ; You-bing XIA
Chinese Acupuncture & Moxibustion 2016;36(5):449-455
The impact of acupuncture for the pregnancy of in vitro fertilization-embryo transfer (IVF-ET) is discussed in the paper. Nowadays there are various conclusions about the impact of acupuncture for IVF-ET, and it may result from the differences in research designs. The effect is closely related to the demographic and clinical characteristics of subjects, such as age, the diagnosis of barrenness, blood flow index of uterine spiral arteries, the cycle of IVF, etc. Besides, the efficacy is influenced by treatment based on syndrome differentiation or not, the frequency and course of acupuncture in both the treating group and the control group, etc. If more reasonable design is achieved in the further study based on them, more reliable evidence will be provided for the effect and mechanism of the pregnancy of IVF-EF by acupuncture.
Acupuncture Therapy
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Embryo Transfer
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Female
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Fertilization in Vitro
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Humans
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Infertility, Female
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physiopathology
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therapy
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Male
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Pregnancy
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Pregnancy Rate
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Randomized Controlled Trials as Topic
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Treatment Outcome
8.Impact of high-load atorvastatin on autoimmunity in patients with unstable angina
Jingyi ZHANG ; Bonan ZHANG ; Yan LI ; Huaibin MU ; Bing LV ; Chunlai ZHANG
Chongqing Medicine 2015;(1):42-44
Objective To explore the impact of high‐load atorvastatin on T cell subsets in patients with unstable angina (UA) after percutaneous coronary intervention (PCI) .Methods One hundred and eighty patients with UA were randomly divided into high‐load atorvastatin group ,ordinary‐load atorvastatin group and routine group ,60 cases in each group .The ratios of CD4+ T cell , CD8+ T cell and the frequencies of CD4+ CD25+ Treg were detected in 3 groups 1 day before PCI and 1 week ,1 month and 6 months after PCI by flow cytometry analysis .Results Different doses of atorvastatin reduced the ratio of CD4+ T cells and in‐creased the ratio of CD8+ T cells and also the frequencies of CD4+ CD25+ Treg after PCI for 1 week ,1 month and 6 months .The longer the time to take atorvastatin ,the more obvious the effect was(P<0 .05) .The ratios of CD4+ ,CD8+ T cells and the frequen‐cies of CD4+CD25+ Treg after PCI for 1 month and 6 months in high‐load atorvastatin showed significant difference compared with those in ordinary‐load atorvastatin group and routine group(P<0 .05) .Conclusion Atorvastatin could regulate the balance of T cell subsets in patients with UA ,and thus it may reduce the UA onset and the treatment effect of high‐load atorvastatin is more sig‐nificant .
9.Aptamer-Based Microcantilever Sensor for O-ethyl S-[2-(diisopropylamino) ethyl] methylphosphonothiolate, Sarin Detection and Kinetic Analysis
Zhiwei LIU ; Zhaoyang TONG ; Lanqun HAO ; Bing LIU ; Xihui MU ; Jinping ZHANG ; Chuan GAO
Chinese Journal of Analytical Chemistry 2014;(8):1144-1149
A new method for O-ethyl S-[2-( diisopropylamino) ethyl] methylphosphonothiolate ( VX) , sarin detection and its kinetic analysis based on piezoresistive microcantilever aptasensor was developed, where VX, sarin aptamers were immobilized on the microcantilever surface by biotin-avidin binding system. A linear relationship between the response voltage and the concentration of VX in the range of 2-60μg/L was obtained. The linear regression equation was △Ue=0. 886C-1. 039 (n=5, R=0. 984, p<0. 001) and the detection limit was 2μg/L ( S/N≥3 ) . A linear relationship between the response voltage and the concentration of sarin in the range of 10-60 μg/L was obtained, the linear regression equation was △Ue=0. 716C-2. 304 ( n=5, R=0 . 996 , p<0 . 001 ) and the detection limit was 10 μg/L ( S/N≥3 ) . The sensor showed no response for O-butyl methylphosphonochloridate, a structural analog of VX and sarin, which indicated high specificity and good anti-interference ability. On this basis, a reaction kinetic model based on receptor-ligand binding and the relationship with output voltage change was established. Response voltage (△Ue ) and response time( t0 ) were obtained from the fitting equation on different concentrations of VX, sarin fitted well with the measured values.
10.Effect of angiotension-Ⅱ on rat cardia fibroblasts
Baoyu LI ; Ying GAO ; Jianying ZHANG ; Bing SHEN ; Congling QU ; Xiaojing MU
Chinese Journal of Pathophysiology 1986;0(01):-
AIM: To investigate the effects of angiotensin-Ⅱ (Ang-Ⅱ) on the proliferation and collagen synthesis in rat cardiac fibroblasts and the expression of Ang-Ⅱ receptor on fibroblasts. METHODS: [3H]-TdR and [3H]-prolin at different concentrations were incubated with the confluent fibroblasts of newborn rats stimulated by Ang-Ⅱ. Receptor of Ang-Ⅱ on fibroblasts and intracellular free calcium ions were examined by autoradiograms and fluorescence technique. RESULTS: In the presence of Ang-Ⅱstimulation, the increase in incorporation of [3H]-TdR and [3H]-prolin was much higher than that of control in a dose-dependent manner. Autoradiograms showed that Ang-Ⅱ was uniformly distributed over the membrane of the fibroblasts. The silver grains on fibroblasts were obviously decreased after adding unlabeled Ang-Ⅱ by the autoradiograms. The concentration of free calcium ions was increased in fibroblast. CONCLUSION: These findings suggest that Ang-Ⅱ promotes fibroblast proliferation and the syntheses of collagen protein, in which Ang-Ⅱ binds to the membrane receptor of fibroblasts to activate the signal system in cells, such as intracellular free calcium ions. [