Adolescent ; Adult ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Cytarabine ; administration & dosage ; therapeutic use ; Female ; Humans ; Leukemia, Myeloid, Acute ; drug therapy ; Male ; Middle Aged ; Prognosis ; Treatment Outcome ; Young Adult

According to the current problems in the management of teaching quality in colleges and universities and from the perspective of teaching total quality management,this paper discussed on the way to construct the diversified,whole and comprehensive teaching quality management system in which both on-campus and off-campus interest parties can participate in and its potential effect on the ba-sis of an empirical study.

AIM　To establish an allele specific PCR amplification (ASA-PCR) for determination of the genotype of CYP2D6*10B polymorphism in Chinese subjects. METHODS　CYP2D6*10B alleles of 65 healthy Chinese subjects were analyzed by a two-step PCR assay and the correlation of genotype and phenotype was studied. RESULTS　There were 20 CYP2D6*10B heterozygous genotypes subjects (wt/m) in 35 very extensive metabolizers (VEMs), which consisted the major part of VEM subjects (57%). Meanwhile, 20 subjects consisting 69% of 29 intermediate metabolizers were CYP2D6*10B homozygous mutant genotypes (m/m). The poor metabolizer was also m/m. The metabolic ratio of CYP2D6*10B m/m subjects were larger than wt/m and wild type, the values were -1.49±0.54, -2.20±0.49 and -2.47±0.61 (P<0.01). CONCLUSION　PCR-ASA was shown to be a rapid and specific method. It can be used to study the genetic polymorphism, especially CYP2D6 intermediate metabolism.

The author analysis the hospital spirit of study,bring forth new ideas,harmonious,benevolence,and developing this spirit in the reformation of hospital.In this way,it will direct and give impetus to the development of hospital,promote getting into the new century.

The pharmacological effects and clinical applications of fish oil preparations were reviewed. Fish oil is a natural material. Its excellent clinical tolerance and multiple pharmacological activities make it a pharmaceutical of broad prospects. Fish oil preparations have been used in the therapy and prevention of hyperlipidemia and atherosclerosis in diabetics. They have also been proved effective in improving symptoms of burns and abscesses. Additionally,fish oil preparations can replace non-steroidal anti-inflammatory drugs in the therapy of immune system disorders and have effects of slowering aging and improving the memory faculty of the aged. Applications of fish oil in organ transplantation have also been reported.

Objective To evaluate the safety and effectis of methylene blue photochemical inactivation of virus in red blood cell (RBC) by using animal models. Methods ①The effects of improving anemia was assessed by transfusing MB P RBC to Beagle’s dog. ②Duck hepatitis B virus(DHBV)was serially diluted and added into human RBC, and then one day old ducklings were inoculated with that treated RBC by intravenous injection. Sera from ducklings were collected and tested by dot hybridization. Infectivity of DHBV (ID 50 ) in RBC was calculated before and after the treatment. Results Dog RBC treated by MB P were not hemolysed after autologous transfusion. There were no changes in serum ALT, AST, BUN, and creatinine after transfusion. The ID 50 contained in RBC changed from 10 3.35 to 10 8.35 after treatment. Conclusion MB P viral inactivation of RBC is safe and effective.

Objective To observe the effects of methylene blue photochemical(MB-P) inactivation of human cytomegalovirus in blood products.Methods Plasma and red blood cell suspensions containing 10% HCMV and 5?mol/L MB were illuminated by fluorescent light of 38000 Lux for 1 hour, HCMV was used as model viruses for validation of virus inactivation.Results The 50% tissue culture infective dose (TCID_ 50 ) contained in plasma and red blood cell suspensions decreased by 4~6 times.Conclusion MB-P treatment is effective in inactivating the infectivity of HCMV in plasma and red blood cell suspension.

Objective To provide evidence for college students'sex education by analysis they status and gender identity of col-lege students'sex knowledge,attitude and behaviors in Guangdong province.Methods By investigating 928 college students of 12 colleges.Results Sex knowledge:Students'awareness of sexual knowledge is low average accuracy rate of question was 37.34%;sex attitude:for sex is a basic human need,love can be sexual,sexual fantasy dreams,masturbation,premarital sex and behavioral aspects of these ideas,the overall degree of recognition is (67.35%,35.24%,56.90%,52.26%,58.04%),boys and girls were sig-nificant differences in acceptance levels(χ2 =7.89,χ2 =48.46,χ2 =189.30,χ2 =32.44,χ2 =27.35,all P <0.01);sex knowledge:students'awareness of sexual knowledge is low;sex behaviors:college Students'heterosexual sex and homosexual sex rates were 8.08% and 1.51%,the incidence of male heterosexual sex is far higher than girls (χ2 = 52.08,P <0.01),there is no significant difference in homosexual acts(χ2 =4.82,P <0.01),condom use rate is low,often use rate was 29.03%,girls was significantly low-er than boys(χ2 = 12.08,P < 0.01 ),they have weak sense of self-protection.Conclusion there are many problems in college students'sex behaviors,attitude and knowledge,and there is need for further strengthening and guidance;we should pay attention to gender differences in education.

Objective To approach the efficacy and safety of arotinolol in treating essential hypertension.Methods The studies about arotinolol in treating essential hypertension were accessed by searching the Cochrane Central Register of Controlled Trials(Issue 3,2008),MEDLINE(1991 to March 2009),EMbase(1991 to March 2009),CBMdisc(1991 to March 2009),and CNKI(1994 to March 2009).The relevant journals and conference proceedings also hand searched.Randomized controlled trials(RCTs) in which arotinolol was used to treat patients with essential hypertension were collected.Then the retrieved studies according to predefined inclusion and exclusion criteria were screened,the quality of included studies was evaluated,and Meta analysis was performed by using RevMan 4.2 software.Results A total of 176 articles were found and 6 of which were finally included.In homogeneity test:?2=4.41,df=7,P=0.73(efficacy);?2=2.96,df=4,P=0.56(safety).In combined test,Z=0.64(P=0.52),OR=1.17,OR95%CI(0.72-1.85)(efficacy);Z=1.75(P=0.08),OR=0.60,OR95%CI(0.34-1.06)(safety).Conclusion There is no significant difference in efficacy and safety between arotinolol and control group in treating essential hypertension.

Objective To observe the clinical effect of budesonide combined with salbutamol in the treatment of children with bronchial asthma,and its influence on oxidative stress and serum interleukin-2 (IL-2),soluble IL-2 receptor (sIL-2r) levels.Methods 70 children with bronchial asthma were divided into observation group and control group according to the different treatment,35 cases in each group.The observation group was inhaled budesonide combined with salbutamol,the control group was given salbutamol inhalation therapy.The clinical efficacy of the two groups was observed,and the changes of serum oxidative stress factor,IL-2 and sIL-2r levels before and after treatment in the two groups were compared.Results In the observation group,20 cases were cured,7 cases were markedly effective,effective in 5 cases,the total effective rate was 94.3％.In the control group,17 cases were cured,6 cases were markedly effective,effective in 4 cases,the total effective rate was 77.1％.The total effective rate between the two groups had statistically significant difference (x2 =26.543,P ＜ 0.05).After treatment,the malondialdehyde (MDA),end products (AOPP) of the observation group were (16.26 ± 1.76)U/L,(42.123 ± 5.745) μmol/L,respectively,which were lower than those of the control group [(22.16 ± 2.84) U/L,(63.63 ± 6.22) μ mol/L,t =23.354,25.348,all P ＜ 0.05].The glutathione peroxidase (GSH-PX),superoxide dismutase (SOD) of the observation group were (236.46 ± 32.25) ng/L,(19.643 ± 3.587) ng/L,respectively,which were higher than those of the control group [(197.43 ± 18.42) ng/L,(18.244 ± 2.446) ng/L,t =18.943,15.123,all P ＜O.05].The IL-2,sIL-2r levels of the observation group were (5.28 ± 2.65) μg/L,(30.376 ± 2.147) mg/L,respectively,which were significantly lower than those of the control group [(9.84 ± 3.22) μg/L,(37.564 ± 1.323)mg/L],the differences were statistically significant (t =21.378,24.643,all P ＜ O.05).Conclusion Inhaled budesonide combined with salbutamol in the treatment of children with bronchial asthma is helpful to control the symptoms of asthma,and can inhibit oxidative stress,reduce serum sIL-2r and IL-2 levels,it is worthy of promotion.