35) and the third strategy of providing diagnosis to those tested positive at screenings in mid-term pregnancy could, respectively, lead to the diagnosis of 0.93 and 1.47 cases of DS per 10 thousand pregnant women, but they could also induce, respectively, 1.58 and 0.28 cases of miscarriage of normal fetuses due to diagnosis procedures. The costs of one DS diagnosis were, respectively, 108890.98 yuan and 443683.11 yuan. Conclusion Taking into consideration both cost-effectiveness and safety factors, priority ought to be given to the third strategy.

Objective In order to discuss the nursing points for chronic skin ulcer patients who had ac-cecpted local oxygen therapy and traditional chinese medicine treatment. Methods We gave totally nursing to 20 patients who accecpted local oxygen therapy and traditional chinese medicine treatment. Results Eighty percent patients had obtained clincal cure and got successful nursing effect. Conclusions Careful perioperation nursing and related monitoring can improve the cute rate of chronic skin ulcer, and then pre-vent complication.

Compounds from Mallotus apelta were isolated by a combination of polyamide columnar chromatography and HPLC method.The compounds' structure were identified by chemical and spectral Methods .Five kinds of flavonoids were isolated from Mall-otus apelta ethyl acetate extracts by HPLC techniques and their structures were determined through spectral analysis(1H,13C-NMR and MS)and comparison with the literature data.Their structures were identified as(1)vicentⅡ,(2)isoschaftoside,(3)schaftoside,(4)apigenin-7-O-β-D-glucopyranoside and(5)apigenin.In addtion, isoschaftoside and schaftoside is the first time obtained from Mallotus apelta.

Adult ; Betacoronavirus ; Clinical Laboratory Techniques ; Coronavirus Infections ; diagnosis ; epidemiology ; etiology ; prevention & control ; therapy ; Diagnosis, Differential ; Extracorporeal Membrane Oxygenation ; Humans ; Pandemics ; prevention & control ; Pneumonia, Viral ; epidemiology ; etiology ; prevention & control ; therapy ; Practice Guidelines as Topic ; Respiration, Artificial

Objective To detect the differential expression profile of microRNAs between patients with or without gall?bladder stone. Methods Samples from 30 patients with gallbladder stones (GS) and 30 without gallbladder stones (GP) were collected, in which microRNAs expression profiles were examined using high-throughput sequencing instrument Illumi?na HiSeq 2500. MicroRNA sequences were obtained and compared to Genebank and Rfam database for classification. Differ?entially expressed microRNAs were screened, and their target genes were predicted. Significant enrichment analysis of GO and KEGG were performed. Real-time quantitative PCR was performed on selected miRNAs in order to validate their expres?sion. Results Clean tags were obtained from both GS group (n=2 215 832) and GP group (n=1 424 770). A total of 17 mi?croRNAs were differentially expressed between GS and GP groups with statistical significance, among which 9 were up-regu?lated and 8 were down-regulated in GS group compared to those in GP group. GO (Gene ocology) analysis showed that target genes were enriched in ion binding and transport, apolipoprotein binding, calcium channel activity, protein kinase activity, steroid hormone biosynthesis and metabolism. KEGG(Kyoto Encyclopedia of Genes and Genomes)analysis is shown for the target genes enriched in cancer related pathways, including WNT, HIPPO pathways. qRT-PCR validation of some differen?tially expressed miRNAs confirmed the result of high-throughput data analysis. Conclusion The differential expression levels of microRNAs may play an important role in occurrence and development of gallbladder stones.

Objective To investigate the feasibility of the virtual source model in Monte Carlo dose calculation for clinical radiotherapy.Methods The Monte Carlo simulation was used to obtain the phase space files which recorded the physical properties of the particles emitted by a medical linear accelerator, and the information on the type, energy spectrum, and distribution of particles were extracted from these files and analyzed to establish the semi-empirical model of virtual two-photon source.The GMC dose calculation engine was used to obtain the 3 cm×3 cm, 5 cm×5 cm, 10 cm×10 cm, 20 cm×20 cm, and 30 cm×30 cm fields of radiotherapy and the results of Monte Carlo simulation of dose distribution in three-dimensional water phantom in 2 intensity-modulated radiotherapy ( IMRT) plans.These results were compared with the results of water phantom measurement or the results of Elekta Monaco planning system to verify the accuracy of Monte Carlo dose calculation based on a virtual source.Results As for the percentage depth-dose distribution curves of the central axis of the water phantom and the off-axis dose curves at different depths in the five fields for radiotherapy, the difference between the results of Monte Carlo simulation and the results of measurement was within 1%.As for the two IMRT plans, the three-dimensional passing rates of Monaco calculation results and Monte Carlo simulation results were 98.9%and 99.4%, respectively, for 3%/3 mm, and 95.1%and 95.4%, respectively, for 2%/2 mm.Conclusions Monte Carlo simulation based on the virtual source model can obtain accurate results of radiotherapy dose calculation.

OBJECTIVEThe aim of this study was to determine the accuracy of personalized implant fabricated via 3D printing and fused deposition modeling technique (FDM) and to compare the results with a real tooth.

METHODSSix prepared extracted orthodontic teeth (in vivo) were scanned via cone beam computed tomography (CBCT) to obtain 3D data and to build the data models by using Mimics 15.0 software. The extracted orthodontic teeth (in vitro) and the personalized implants designed via 3D printing and FDM were scanned via CBCT to obtain data and to build the data models at the same parameters. The 3D deviations were compared among the in vivo teeth data models, in vitro teeth data models, and printing personalized implant data models by using the Geomagic studio software.

RESULTSThe average deviations of high and low areas between date models of in vivo teeth and personalized implants were 0.19 mm and -0.16 mm, respectively, and the average deviations between in vitro and in vivo teeth were 0.14 mm and -0.07 mm, respectively. The independent t test showed that no statistically significant difference was observed between the two groups (P>0.05).

CONCLUSION1) The personalized dental implants were manufactured via 3D printing and FDM with a high degree of precision. 2) Errors between the data models of in vitro and in vivo teeth were observed at the same CBCT parameters.

Objective To evaluate the effects of celecoxib on tumor growth and cell apoptosis in human triple-negative breast cancer (TNBC) xenografts in nude mice.Methods Human TNBC MDA-MB-231 cells were inoculated subcutaneously into BALB/c nude mice.The mice (n=32) were then randomly divided into 4 groups,the control group and the celecoxib group (receiving 25,50,100 mg·kg-1·d-1 respectively).At the end of the study,tumor tissues were collected and tumor volume was measured.Cell apoptosis was determined by flow cytometry (FCM) analysis.NF-κB p65 and pS0 protein levels were measured by immunohistochemistry.Caspase-3 and survivin protein levels were detected by western blotting.Results celecoxib at dose of 25,50 and 100 mg·kg-1·d-1 inhibited the tumor growth significantly,compared with the control group.FCM results showed that apoptotic rates were (13.58±3.16) ％ and (21.91±4.75) ％ in moderate and high dose of celecoxib-treated group,compared with (3.15±1.73) ％ in control group (t =6.736,P ＜ 0.05;t =12.151,P ＜ 0.05).p65 expressions were 79.3 ％,46.7 ％ and 23.9 ％ in low,moderate and high dose of celecoxib-treated group,compared with 89.7 ％ in control group (x2 =3.312,P ＜ 0.05; x2 =10.785,P ＜ 0.05;x2 =15.900,P ＜ 0.05).Besides,western blotting analysis demonstrated that celecoxib significantly downregulated survivin expression,while upregulated the active form of caspase-3 expression.Conclusion Celecoxib could suppress TNBC tumor growth and induce cell apoptosis,which might be partially associated with inactivation of p65 and downregulation of survivin.

With the progresses of the research on "Digital Human", more and more information has been needed for the setting up of the three-dimensional digital models of human organs. In the present paper, based on the method of block mapping with a normal-style bump mapping method, we normalized the vector of the surface of the models, computed offset of the texture coordinates and added to them. The projection of the coordinates to the tangential space complemented the disturbance to the vector of models' surface. The method was proposed with bump texture mapping on the surface of the biological models of organs to improve the impression of the visualization of the organ models and to enhance the sense of reality of the models.
Humans ; Models, Anatomic ; Models, Biological

Ear ; Eosinophilic Granuloma ; Facial Neoplasms ; Humans ; Male ; Middle Aged ; Parotid Gland