Objective:To investigate the expression and the effects of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock.Methods:18 healthy rabbits were randomly divided into control group(group A),hemorrhagic shock 1 hour group(group B) and hemorrhagic shock 2 hours group(group C),with 6 rabbits in each group.Except the rabbits in the control group,rest rabbits were made into hemorrhagic shock models with lung injury according to Wiggers modified method.TNF-?、IL-10 in plasma were measured by ELISA.Expressions of CD40 and CD40L in lung tissue were measured by immunohistohemistry.Water quantity and pathological change in the lung were observed.Results:The lung of group B and C displayed obvious injury,water quantity increased(P

OBJECTIVE To learn about the state of disinfecting quality and its impact factors of our hospital in recent 10 years.METHODS According to GB15982-1995 and GB15981-1995 the sampling and surveillance were carried out to the objects concerned for disinfecting in the hospital.RESULTS It showed ascent trend in its entirety for the qualified rate of sample surveillance(70.53-96.34%).The qualified rates of applying disinfecting liquid,medical treatment necessities and pressure steam sterilizer were also higher in the surveillance each year.CONCLUSIONS The lows and regulations correlated with disinfection and operating technological specification have become more and more perfect and flawless,supervision and management also have been intervened and strengthened,and the level of the consciousness and knowledge of disinfection among the staff have had elevation,all these have elevated the disinfection job of the hospital in quality.

Diabetic retinopathy (DR) is a common complication of eye in diabetics and is one of the major causes of blindness in adults.The current treatments of DR include surgery and laser therapy,but this does not fundamentally cure DR.Recent studies have found that mesenchymal stem cells (MSCs) for cell therapy to DR have a wide range of applications.MSCs have regenerative potential and play a role in the repairment of the retina,but the survival and homing ability of MSCs are poor after transplantation,which reduces the therapeutic efficiency of MSCs.Statins have a variety of beneficial effects independent of lipid regulation,which can promote the proliferation of MSCs and inhibit the apoptosis of MSCs.This paper reviewed the application and mechanism of MSCs combined with statins in the treatment of DR.

Objective: To evaluate the therapeutic efficacy of acupuncture plus Chinese medicinal fumigation and washing in treating edema of the affected limb in post-stroke hemiplegia. ＜br> Methods: Two hundred patients with edema of the stroke-affected limb from our hospital were randomized into two groups according to their visiting sequence. In the observation group, 100 subjects were intervened by acupuncture plus Chinese medicinal fumigation and washing in addition to oral administration of diuretics, while the other 100 subjects in the control group were only given diuretics. The therapeutic efficacy was evaluated after successive 2-week treatments. ＜br> Results: The total effective rate was 90.0% in the observation group, significantly higher than 66.0% in the control group, indicating that there was a significant difference in comparing the overall therapeutic effect (P＜0.01). ＜br> Conclusion: Acupuncture plus Chinese medicinal fumigation and washing in addition to oral administration of diuretics is effective in treating edema of the affected limb in post-stroke hemiplegia.

ObjectiveTo identify the proteins presented variation during ischemia/reperfusion injury (I/RI) in liver transplantation (LT).MethodsAt Mar.2009,a total of nine liver sample tissues,three samples obtained from each donor liver at three different time points,were studied.The time points were as follows:(1) the time point immediately after donor liver was harvested (T1) ; (2) the time point just before the anastomosis of hepatic artery and potal vein (T2),during this time period the donor liver was kept in ice cold and transported,then back-table prepared,during time from T1 to T2,ischemia injury occurred; (3) the time after donor reconnected to the recipient (T3),during which the donor liver was subjected to reperfusion injury.The three time points represent normal control,ischemia injury,and reperfusion injury respectively.By using method of 2DE-MALDI-TOF and mass-spectrum,the differential expression of these protein at T1,T2,and T3 time point were compared.ResultsFrom the 1580 proteins that were isolated,19 ischemia/reperfusion injury (I/RI)-related proteins that varied markedly from time point T1 to T2 and from time point T2 to T3 were identified.They were metabolic enzyme,molecular chaperone,redoxase,cytoskeleton protein,signal transduction protein,and binding protein,respectively.The character of each protein was further analized based on their functions.ConclusionsFor the first time,we detected the protein related to I/RI during LT.We obtained independent proteomics information of ischemia injury,and reperfusion injury,respectively.Further functional analyses of these proteins may improve the understanding of the critical biological processes during I/RI.Medicine intervention on the I/RI-related proteins may result in improved survival of the recipients.

Objective To explore the relationship between nuclear factor ?B (NF ?B) and the occurrence, metastasis, and treatment of colon cancer. Methods The literature on the structure and the property of molecular biology of NF ?B, the relationship between NF ?B and apopotosis, malignant tumor and colon cancer were reviewed.Results NF ?B had action of antiapopotosis. The occurrence of malignant tumor had close relation with the oncogene by NF ?B, the metastasis of malignant tumor was that cell of cancer escaped the killing and supervising of immunity by NF ?B. NF ?B affected the occurrence and metastasis of colon cancer by regulating c myc, Cox2, ICAM 1.Conclusion NF ?B has important action in the occurrence and metastasis of colon cancer. It will become a new target of treatment.

Objective To introduce the new nomenclature scheme of the International Working Group (1995) on hepatic nodules, and summarize the imaging features of various hepatic nodules in light of their pathological characteristics, and evaluate the diagnostic values of various imaging facilities.Methods Ultrasound, computed tomography(CT), magnetic resonance imaging(MRI), and angiographic CT were reviewed and introduced.Results Many of these types of hepatic nodules play a role in the de novo and stepwise carcinogenesis of hepatocellular carcinoma(HCC) in the following steps: regenerative nodule, low grade dysplastic nodule, high grade dysplastic nodule, small HCC, and large HCC. Accompanying such transformations, there are significant alterations in the blood supply and perfusion of these hepatic nodules.Conclusion Modern state of the art medical imaging facilities can not only delineate and depict these hepatic nodules, but also provide important clues for the characterization of focal hepatic lesions in most cases, thus facilitating the early detection, diagnosis and management of HCC in its early stage.

Objective To introduce the technique of three dimensional portal venography of multi detector row spiral CT and its clinical application in the evaluation of the portosystemic collateral shunts of liver cirrhosis. Methods All relevant literatures were retrospectively reviewed on the application of two dimensional and three dimensional reconstruction techniques such as MIP, SSD, VRT of multi detector row spiral CT to demonstrate the collateral vessels of liver cirrhosis.Results The distribution, pathway and anatomy of portosystemic collateral vessels were well shown by multi detector row spiral CT portal venography. Conclusion Multi detector row spiral CT portal venography provides excellent depiction of the anatomic characteristics of the collateral shunts and enables the continuous tracing of vascular structures, thus it is very helpful in the imaging evaluation of the collateral vessels of liver cirrhosis.

Objective To investigate the effects of P27Kip-1 on proliferation of airway smooth musde and airway remodeling in asthmatic rats.Methodes 30 healthy,male SD rats were randomly and equally divided into three groups :control group(C),asthmatic 4 weeks group(A1),asthmatic 6 weeks group(A2).A rat asthma model was developed by the sensitized with ovalbumin and Al(OH)3,and repeatedly exposed to aerosolized ovalbumin.After the last challenge,The animals were evaluated for AHR,the airway wall thickness(Wat /Pbm)and the bronchial smooth muscle thickness(Wam/Pbm)were measured by image analysis system.The expression of P27Kip-1 and PCNA was determined by immunohis-tochemical staining.Results(1).The expression of P27Kip-1 protein in two asthmatic groups was significantly lower than that of the control group,respectively(P

ObjectiveTo introduce the effect of trifoil peptides on the protection and treatment of gastrointestinal ulcer(GIU). MethodsWe reviewed literatures about trifoil peptide in its structure, distribution, physiologic effect and mechanism, and its relation with GIU. Results Trifoil peptides formed a layer of mucus glue by combining with glycoprotein ,and improved the defense ability of the gastrointestinal(GI) mucosa .Trifoil peptides repaired the GI mucosa by abjusting the movement of mucosal cells. Trifoil peptides also had the effect of antiproliferation and antiapopotosis. There was an ulcer-associated cell lineage (UACL) around GIU. In GIU, trifoil peptides unusually expressed new cells coming from UACL moved to the surface of ulcer, and repaired the ulcer. UACL can express three trifoil peptides.Used before and after the experiment, trifoil peptides have the protective and therapeutic effect on the experiment gastroenteric ulcer. ConclusionsTrifoil peptides may offer a new method to prevent and treat GIU.