1.Repairing effect of pedicle omentum in severe ureteral injury
Yubin CAI ; Hailun ZHAN ; Bin MIU ; Dan HE ; Tujie SI ; Xin GAO
Chinese Journal of Urology 2009;30(7):454-456
Objective To evaluate the repairing effect of pedicle omentum on severe ureteral in-jury. Methods Twenty healthy dogs were randomized into the experiment group and the control group. Firstly the model of severe ureteral injury was made. In experimental group, the pedicle omen-turn were used to wrap up the severe injured ureter, but which was no done in control group. Urinary fistula and ureteral necrosis were observed. At 12 weeks postoperatively,the models were re-operated to investigate whether the severe injured ureters had healed. Angiogenesis, VEGF and its receptor KDR were also examined in stoma and surrounding tissue histopathologically. Results No urinary fistula was observed in experimental group, but 2 cases in control group were dead because of recurrent abdominal cavity infection after the formation of urinary fistula. The mucosa and smooth muscle of stoma were completely regenerated, and the blood vessels were regenerated more significantly in expe-rimental group than in the control group. In experimental group, the VEGF and KDR were over-ex-pressed, and the positive cell rates were (12. 65±0. 02)% and (10. 23±0. 03)%. But in control group, the stoma were not healed, and severe ureters1 strictures were observed in all dogs. Angiogene-sis was not signifieant, the VEGF and KDR positive cell rates were (1.54±0. 03)% and (2. 654± 0.04)%, respectively. Conelusions Pedicled omentum can promote the repair of severe ureteral in-jury. The mechanism may be the over expression of VEGF and KDR in prompting angiogenesis.
2.In vivo concentration gradient of basic fibroblast growth factor after coronary venous retrograde perfusion
Lei ZHEN ; Xiao WANG ; Huangtai MIU ; Shibin QIAO ; Xingxin WU ; Yan QIAO ; Baiqiu LIU ; Xinmin LIU ; Bin QUE ; Shaoping NIE
Chinese Journal of Tissue Engineering Research 2013;(24):4473-4480
10.3969/j.issn.2095-4344.2013.24.015