ObjectiveTo identify the proteins presented variation during ischemia/reperfusion injury (I/RI) in liver transplantation (LT).MethodsAt Mar.2009,a total of nine liver sample tissues,three samples obtained from each donor liver at three different time points,were studied.The time points were as follows:(1) the time point immediately after donor liver was harvested (T1) ; (2) the time point just before the anastomosis of hepatic artery and potal vein (T2),during this time period the donor liver was kept in ice cold and transported,then back-table prepared,during time from T1 to T2,ischemia injury occurred; (3) the time after donor reconnected to the recipient (T3),during which the donor liver was subjected to reperfusion injury.The three time points represent normal control,ischemia injury,and reperfusion injury respectively.By using method of 2DE-MALDI-TOF and mass-spectrum,the differential expression of these protein at T1,T2,and T3 time point were compared.ResultsFrom the 1580 proteins that were isolated,19 ischemia/reperfusion injury (I/RI)-related proteins that varied markedly from time point T1 to T2 and from time point T2 to T3 were identified.They were metabolic enzyme,molecular chaperone,redoxase,cytoskeleton protein,signal transduction protein,and binding protein,respectively.The character of each protein was further analized based on their functions.ConclusionsFor the first time,we detected the protein related to I/RI during LT.We obtained independent proteomics information of ischemia injury,and reperfusion injury,respectively.Further functional analyses of these proteins may improve the understanding of the critical biological processes during I/RI.Medicine intervention on the I/RI-related proteins may result in improved survival of the recipients.

AIM　To improve the treatmemt efficacy and reduce the side effect of carboplatin (CBP), the antitumor drug was incorporated into niosomes (NS). METHODS　Lung targeted niosomes of CBP (CBP-NS) were prepared by the method of hand shaking. The ultraviolet absorption spectrophotometric method was used for the determination of CBP. The method of dynamic dialysis was used for in vitro release of CBP from CBP-NS. The S-180 lung neoplasms models were established by iv cancer cells in mice. The number of pulmonary nodules was examined for evaluating the therapeutic efficacy. RESULTS　The data showed that the mean diameter of CBP-NS was 3.72 μm, with a span of 0.66. The entrapment ratio of CBP in CBP-NS was 29.2% and the CBP-NS were stable for three months stored at 3℃-5℃, 15℃-25℃ or 37℃ (relative humidity 75%). The release profile in vitro could be described by a biexpotential equation. The calculated values of the three targeting parameters indicated that CBP-NS showed good targeting efficiency. The results of therapeutic trials showed that the antitumor effects were significantly increased by injection of CBP-NS compared with CBP in the treatment of mice with lung carcinoma. CONCLUSION　The results indicated that CBP-NS have good targeting efficiency in vivo, and the biodegradable CBP-NS may decrease the side effects of carboplatin and improve its therapeutic efficacy.

AIM　To establish innovational triangular phase diagrams for preparation of pharmaceutical microemulsion (ME) with low toxicity as a result of reducing the amount of the emulsifiers. METHODS Lecithin and polyoxyethylene octyl phenyl ether (OP) were used as emulsifiers (E), absolute alcohol and n-octyl alcohol as assistant emulsifiers (A), ethyl oleate and olive oil as the oil phase (O). For the innovational triangular phase diagrams, lecithin-ethyl oleate-water and absolute alcohol (optimized ratio W/A=0.6)system, and OP(olive oil and n-octyl alcohol (O/A=0.34)-water system were used for preparation of the ME. While for the traditional triangular phase diagrams, OP and n-octyl alcohol (E/A=Km=1.5)-olive oil-water system, lecithin and absolute alcohol (E/A=Km=1.12)-ethyl oleate-water system were used. Then, the corresponding amounts of the emulsifiers used in both methods were compared. RESULTS　In the ME of lecithin systems by the innovational and the traditional methods, the amounts of emulsifier are 6%-28% and 28%-29.6%, respectively. In the ME of OP systems by the innovational and the traditional methods, the amounts of emulsifier are 23%-40% and 27.6%-49.8%, respectively. CONCLUSION　The method of innovational triangular phase diagram for preparation of ME is superior to the traditional method in finding out the lower amounts of emulsifiers for ME, and is a feasible method with guiding significance for preparing ME.

The therapy of removing beat and toxic,strengthening the body to cultivate the fundamental is the mainstream of treating cancer in traditional Chinese medicine up to now,and the therapy of warming yang has been rarely involved.While the pathogenesis of tumor is spleen and kidney yang deficiency in root,and phlegm,stasis,and poison entangled with each other at the secondary position; the main therapeutical principle of tumor is simultaneous application of purging-tonifying therapy while focusing on protecting yang.The author believes that the treatment of cancer should pay attention to the whole concept,especially warming yang for patients with advanced tumors.The therapy of warming yang is an effective way to get rid of phlegm and blood stasis.Patients after radiotherapy and chemotherapy should be paid more attention to warming yang.

Objective:To explore the effect of Zinc finger protein 185 (ZNF185) on the migration of human gliocytoma cells. Methods: ZNF185 protein expression in human gliocytoma and its adjacent normal tissues was detected through Western blot. The ZNF185 gene was cloned and transfected into the human gliocytoma cell line SF767. ZNF185 cells were located through immunofluo-rescence staining, and cell migration was analyzed using the cell scoring method. Results:CZNF185 protein expression was lower in the human gliocytoma cell line than in normal para-neoplastic tissues. ZNF185 protein was mainly expressed in the cytoplasm and cell membrane of the human gliocytoma cells and was co-located with F-actin. ZNF185 overexpression repressed the migration of human gliocytoma cells. Conclusion:ZNF185 represses the migration of human gliocytoma cells by binding to F-actin.

OBJECTIVE:To investigate the effects of Dexmedetomidine hydrochloride injection on elderly patients underwent thoracic surgery during anesthesia recovery period. METHODS:Totally 60 elderly patients underwent thoracic surgery in our hospi-tal during Jun. 2014-Feb. 2016 were selected as research objects and then divided into control group and observation group ac-cording to even and odd-numbered admission order,with 30 cases in each group. Control group was given Propofol injection 0.5-1 mg/kg intravenously 5 min after mask oxygen inhalation. Observation group was given Dexmedetomidine hydrochloride injec-tion 0.5-1.0 μg/kg intravenously. Both group were successively given Etomidate emulsion injection 0.1-0.2 mg/kg,Sufentanil citrate injection 0.3 μg/kg and Cisatracurium besilate for injection 0.2 mg/kg intravenously for anesthesia induction;tracheal intubation was performed and ventilator was used to assist ventilation after anesthesia induction. And then control group was given continuous intravenous dripping of propofol 3-6 mg/kg·h;observation group was given continuous intravenous dripping of Dexmedetomidine hydrochloride injection 0.2-0.5 μg/(kg·h). At the same time,both group were given continuous intravenous dripping of sufentanil 0.1-0.2 μg/(kg·min)and cis-atracurium 1.5-2 μg/(kg·min),and continuously inhaled 1%-2% sevoflurane for anesthesia mainte-nance. Hemodynamic index(HR,MAP,SpO2),postoperative recovery,the occurrence of delirium,restlessness,cough and involun-tary movement were observed in 2 groups before surgery (T0),after anesthesia induction (T1),at the moment of sputum suction and extubation(T2),5 min after extubation(T3),30 min after extubation(T4). RESULTS:At T2 and T3,HR and MAP of control group were increased significantly compared to at T0,T1,and higher than those of observation group,with statistical significance (P<0.05). At T0,T1,T4,there was no statistical significance in HR and MAP between 2 groups (P>0.05). At T0,T1,T2,T3, T4,there was no statistical significance in SpO2 level between 2 groups(P>0.05). The breathing recovery time,eye openning time and extubation time of observation group were significantly shorter than those of control group,with statistical significance (P<0.05). The incidence of postoperative restlessness,delirium,cough and involuntary movement in observation group were signifi-cantly lower than control group,with statistical significance (P<0.05). CONCLUSIONS:Dexmedetomidine hydrochloride injec-tion can keep hemodynamics stable in elderly patients underwent thoracic surgery during anesthesia recovery period.,shorten recov-ery time and reduce the occurrence of stress response as postoperative delirium,restlessness,cough,involuntary movement.

Objective To study the clinical characteristics and misdiagnostic causes of acute perforation of peptic ulcer (APPU) in children ,ao as to improve the diagnosis and treatment . Methods The clinical data of 26 cases of APPU in children were analysed retrospectively. Results The clinical manifestations include abdominalgia , abdominal distension, nausea and vomiting, fever; tension of abdominal muscle , epigastrium tenderness, rebounding pain,and gurgling sound attenuation or disappearance . The diagnosis was made preoperatively in 10 cases , and misdiagnosed in the other 16 cases . All patients underwent perforation repairing operation . There was no mortality in this series. All the patients were followed up for 1 to 2 years,and only 2 cases (7.7%) had peptic ulcer reccurrence. Conclusions Peptic ulcer in children often occurrs in schoolage and most of ulcers were duodenal ulcer. The clinical manifestation of APPU in children has no specific clinical presentation . The early diagnosis of APPU in children is difficult. Combined use of X ray with B ultrasonic examination can apparently improve the accurate rate of diagnosis. Once the diagnosis is made ,the surgical treatment should be performed as early as possible. The perforation repair should be chosen at first. Anti-Hp treatment should be given postoperatively.

Food allergy( FA) is a growing health problem which affects young children′s growth. Re-cent studies have showed that leukotrienes can contract gastrointestinal smooth muscle,increase intestinal perm-bility,and it is obviously related to the development of FA gastrointestinal disorder. Leukotriene receptor antago-nists have provided a good therapeutic option and showed clinical benefits in the management of the FA gastroin-testinal disorder.

Objective To investigate the predictive factors of acute pancreatitis for patients with gallstone.Methods One hundred and eighteen patients with gallstones who were admitted to Department of Gastroenterology,People's Hospital of Wuhan University were selected,the gallbladder size,number and size of gallstones,common bile duct stones were determined by imaging study.Then the patients were grouped according to the complication of acute pancreatitis.Results Sixty-one cases of acute pancreatitis occurred out of 118 patients.The gallbladder size of 74 patients was normal,and among them 49(66.2％) developed acute pancreatitis; the gallbladder size of 44 patients was increased or decreased,and among them 12(27.3％)developed AP.Thirty-one patients had a single gallstone,and 11 (35.5％) developed AP; while 87 patients had multiple gallstones,and 50 (57.5％) was complicated with AP.Among patients with AP,11 patients had a single gallstone,and the size of the gallstone in the 8 patients (72.7％) was ≥10 mm; while 50 patients had multiple gallstones,and the size of the gallstone in the 41 patients (82.0％) was ＜ 10 mm.Nineteen patients were complicated with common bile duct stones,and 17 (89.5％) developed AP; while 99 patients were not complicated with common bile duct stones,and 44 (44.4％) developed AP.The difference between the two groups was statistically significant (x2 =16.758,P =0.000 ; x2 =4.425,P =0.029 ; x2 =13.434,P =0.001 ; x2 =12.994,P =0.000).Conclusions Acute biliary pancreatitis is associated with gallstones.The size of gallbladder,size and number of gallstone,as well as the presence of common bile duct stones are related to the development of AP.

Objective To investigate the effect of medialization of the patella component on patel-lar-femoral track and clinic result in patellar resurfacing arthroplasty in Chinese adults.Methods From September2001to March2002,patellar-femoral track of medialized patella component in total75consecu-tive total knee arthroplasties were checked with"no thumb test"sign during patellar resurfacing as well as af-ter patellar resurfacing.The patients consisted of 8males(14knees)and40females(61knees).The average age of the patients was66.2years old(ranging from62to79years old).The preoperative diagnosis of the knees were osteoarthritis96%,rheumatoid arthritis4%.There were65varus knees,9nomal knees and1val-gus knee.Eight knees had patellar subluxation or bad patellar-femoral track before arthroplasty.Results Patellar-femoral track were checked with"no thumb test"sign after putting femoral and tibial tray before patellar resurfacing,69of 75knees could reach"no thumb test".After resurfacing and medialization of patella com ponent by2to5mm,all69knees could still reach"no thumb test".But 6knees were ineffectual.After resur facing and medialization of patella component by4to8mm,5of the6knees could reach"no thumb test",the remained one reached"no thumb test"and was of 15?valgus after lateral release.Average flexion of total75knees was108.6?(from90?to115?)two weeks after operation,and increased to116.5?(from95?to125?)after an average follow-up of 15months,ranging from12to18months.No case had patellar fracture insta bility and patellar subluxation.Conclusion Medialization of the patella component in total knee arthro plasty can improve patellar-femoral track,reduce requirement of release of patellar lateral retinac-ulum,decrease patellar-femoral joint complication and increase survival ship of prosthesis.