Objective To observe the clinical efficacy of Tong Du Wen Yang (unblocking the Governor Vessel and warming yang) in treating amyotrophic lateral sclerosis (ALS).Method Twenty-eight eligible ALS patients were randomized into two groups by following the visiting sequence. Thirteen patients in the control group were intervened by orally taking Riluzole tablets; 15 cases in the treatment group were by Tong Du Wen Yang needling in addition to oral administration of Riluzole tablets. Before treatment and after 6-month treatment, the therapeutic efficacies were evaluated by using the Chinese medicine syndrome and sign scoring for Wei-flaccidity diseases and Appel function scale.Result After the treatment, the Chinese syndrome and sign scoring scores for Wei-flaccidity dropped in both groups, and the decrease in the treatment group was more significant than that in the control group (P<0.05). The Appel scores dropped in both groups after the treatment, while the decrease in the treatment group was less significant than that in the control group (P<0.05).Conclusion Tong Du Wen Yang needling plus orally taking Riluzole tablets can produce a more significant efficacy than using Western medication alone in treating ALS.

Objective To investigate the effect of spironolactone combined with valsartan on renal function in patients with early diabetic nephropathy.Methods 158 early diabetic nephropathy patients from May 2014 to June 2015 in department of endocrinology of Nanxun People’s Hospital of Huzhou were selected and divided into control group (79 cases) and experimental group (79 cases).The control group were treated by valsartan and the experimental group were treated with spironolactone on the basis of control group.The changes of renal function, potassium ion and blood rheology were observed and compared after treatment.Results Compared with control group post-treatment, the systolic pressure and diastolic pressure levels were lower, plasma urinary albumin excretion rates (UAER) level was lower (P <0.05), albumin (Alb), serum creatinine (Scr), blood urea nitrogen ( BUN) , creatinine clearance ( Ccr) and K +levels had no significant differences, whole blood specific viscosity, plasma specific viscosity and whole blood specific reduced viscosity were lower ( P <0.05 ) in experimental group.Conclusion Spironolactone combined with valsartan could effectively improve clinical efficacy of patients with early diabetic nephropathy , improve the renal function of patients, recover ion disorder and reduce blood viscosity.

Objective To explore the molecular mechanism of hypophrenia induced by Down syndrome (DS).Methods Ts65Dn mice were used as DS animal model.Three female mice and three male mice of three to twelve weeks old were mated.Among the 17 first-generation mice alive,five mice remained Ts65Dn trisomy and 12 mice were normal.Five Ts65Dn mice and five normal mice were selected randomly as Ts65Dn group and control group,and bred till 16 to 18 weeks old for experiments.Differential proteins in hippocampus of mice were tested by isobaric tags for relative and absolute quantitation (iTRAQ).Expressions of the differential proteins in Ts65Dn group were detected compared with those in control group.Results A total of 2805 proteins were identified in hippocampus of Ts65Dn group and control group,and significant differences were observed in the expressions of 374 proteins.Compared with those in control group,expressions of 195 proteins increased and 179 reduced in Ts65Dn group.Sorted by P value from low to high,the seven proteins with the lowest P value were uncharacterized protein C2orf47 homolog,isoform 2 of filamin A-interacting protein 1-like,zinc finger protein,isoform 1 of pericentriolar material 1 protein,SEC23 interacting protein,BAG family molecular chaperone regulator 3 and serpin H1.Conclusions Differential proteins are observed in hippocampus of Ts65Dn mice,perhaps closely correlating to neurological defects.The new technology of iTRAQ helps to screen and identify differential proteins in hippocampus.

BACKGROUND: It has been obtained by the animal experimental and clinical researches that electroacupuncture promotes the regeneration of peripheral nerve, but its mechanism is still unknown. Neurotrophic factor (NF) maintains the survival of neurons in injured nerve and promotes the axonal regeneration. The observation of the changes of nerve growth factor (NGF) before and after electroacupuncture stimulation probably provides a new approach on revealing peripheral nerve disorder.OBJECTIVE: To observe the effects of electroacupuncture on NGF in regeneration microenvironment after facial nerve injury.DESIGN: Complete randomized-division and controlled animal experiment.SETTING: Department of Acupuncture-Moxibustion of West China Hospital affiliated to Sichuan University.MATERIALS: The experiment was performed in Animal Experiment Center of West China Hospital affiliated to Sichuan University from September to December 2001, in which, 50 New Zealand healthy adult rabbits were employed and randomized into 2 groups, named experiment group and the control, 25 rabbits in each one.METHODS:① After intravenous anesthesia on experimental animal,the upper buccal branch of facial nerve was separated and exposed. Under surgical magnifying glass, the nerve was cut off and a silica-gel tube was used to be inlayed in two broken ends and fixed with stitching, by which, a regeneration chamber is formed. In experiment group, on the day when the rabbit was completely resuscitated from anesthesia in the operation, electroacupuncture was applied. Points: Yifeng (TE 17), Dicang (ST 4), Jiache (ST 6) and Hegu (LI 4). Method of treatment: perpendicular puncture was applied on Yifeng (TE 17), 1 cm deep; penetrating puncture was from Dicang (ST 4) to Jiache (ST 6), 1.5 cm deep; and on Hegu (LI 4), 0.5 cm deep. The electrodes was attached on Yifeng (TE 17) and Dicang (ST 4) respectively, with dense-disperse wave, at 18-20 Hz, 1.5 V. The needles were retained for 30 minutes and the treatment was given once a day, totally for 14 days.In the control,no any management was applied.② On the 3rd, 5th 7th, 10th and 14th days after surgery, 10 animals were sacrificed, 5 rabbits in each of experiment group and the control. The fluid was collected from the regeneration chamber and double antibodies sandwiched enzyme-linked immunosorbent assay (ELISA) was used to determine NGF level in regeneration chamber. ③ t test was adopted for the difference comparison of measurement data.MAIN OUTCOME MEASURES: Comparison of NGF level in regenerated chamber on the 3rd, 5th, 7th 10th and 14th days after surgery RESULTS: Fifty rabbits were all in result analysis. From the 3rd day to 7th day after surgery, NGF level in regeneration chamber was not different remarkably between experiment group and the control (P ＞ 0.05). On the 10th and 14th days, NGF level in regeneration chamber in experiment group was higher remarkably than that in the control [On the 10th day after surgery: (2 793.0±163.1), (2571.1±91.6) ng/L; on the 14th day after surgery: (2 696.1±147.5), (2 243.7±271.2) ng/L, t=4.45, 3.44, P ＜ 0.01].On the 7th day after surgery, NGF levels in regeneration chamber in both experiment group and the control were up to the peak. But, since the 14th day after surgery, NGF level in the control began declining, that in experiment group was still kept at a relatively high level.CONCLUSION: Electroacupuncture improves and maintains the stable high level of NGF in regeneration microenvironment after facial nerve injury, which is probably one of the mechanisms of electroacupuncture on promoting the regeneration of peripheral nerve.

Objective To explore a simple and effective way of establishing a 30 % small-forsize liver transplantation in rats. Methods SD rats were selected as the donors and recipients. Smallfor-size orthotopic live transplantation was performed using Kamada's two-cuff method. Donor's liver was flushed via abdominal aorta and hepatectomy in situ was done. Animals were divided into 3 groups (40 pairs of rats in each): group Ⅰ , median lobe was used as graft; group Ⅱ, right of median lobe and right lobe were used as graft; group Ⅲ, median and right lobes were used as graft. The body weight of the donor was the same as the recipient in groups Ⅰ and Ⅱ , but 100～ 120 g less than in group Ⅲ. The operating time, 7-day survival and technical complications were compared among these 3 groups. Results The operating time of hepatectomy was shorter in group Ⅲ than in groups Ⅰ and Ⅱ (8. 8±0.7 vs 11.5± 1.1 vs 10.1 ±1.0 min, P＜0.01). The cold ischemia time of graft, the anhepatic time, the operating time of recipient and the transplanting successful rate showed no significant difference among the 3 groups. Compared with groups Ⅰ and Ⅱ , the incidence of bleeding,bile leakage and IVC stricture was significantly decreased in group Ⅲ (P＜0. 05). Other complications after operation showed no significant difference among the 3 groups (P＞0. 05). Group Ⅲ had more 7-days survivors and longer median survival time, but there was no significant difference among the 3groups. Conclusion Small for donor body weight with median and right lobes as graft was a more effective and simple way of establishing a 30 % small-for-size liver transplantation in rats with shorter hepatectomy time and less complications after operation.

Cancer stem cells are a new field with the gradual deep understanding of the stem cell research. Although its history is not long, the rapid development showed a good potential prospect. The existence of cancer stem cells and its use as tumor formation, growth and metastasis-based approach has been widely recognized. In theory, cancer stem cells derived from normal stem cells can transform. Cancer-causing factors can also be derived-from the dedifferentiation induced cells. Because it contains all-round, multi-and single stem cell, with the proliferation and differentiation potential of different directions, it is possible to explain the heterogeneity of tumor cells, and reasons for hypoxic environment, anti-cancer drugs and radiation resilience. In this area, it has been made a lot of progress in recent years. However, there are still some problems to be solved. Such as the identification of cancer stem cells, biological function and mechanism.

0.05).Conclusion Omeprazole and Tegaserod improved the reflux symptoms of the NERD patients.Omeprazole improves abnormal acid exposure but not abnormal bile exposure.Tegaserod improved neither abnormal acid exposure nor abnormal bile exposure.

Aim To construct a screen assay of ?-secretase inhibitors.Methods A human ?-secretase cDNA was cloned by RT-PCR and then reconstructed in an expression vector.The recombinant ?-secretase fusion protein was expressed and purified from Escherichia coli.A screen assay of ?-secretase inhibitors was constructed in vitro using a recombinant A ?-fusion protein as substrate.The enzyme reaction was detected by SDS-PAGE and an ELISA method using metal chelating plates to catch the substrate.Results A human ?-secretaae cDNA was cloned and expressed in escherichia coli.The ?-secretase enzyme reaction can be detected by two kinds of method: SDS-PAGE and ELISA.The IC_(50) for an inhibitor statVal determined by this method was in good agreement with those reported in the literature.Seventy seven species of Chinese traditional medical plants were screened using this method and seventeen species show weak inhibitory activity.Conclusion A screen assay of ?-secretase inhibitors was successfully constructed and can be used to screen natural products.

Objective To investigate the beneficial effect on enterocyte-like cells HT-29 through adhesion of Lactobacillus. Methods Using viable bacteria counting method to evaluate inhibition of Escherichia coli adhesion and invasion to enterocyte-like cells by incubation with Lactobacillus together. Cellular membrane permeability and cell viability were examined in vitro by LDH level and MTT method respectively. Results Adhesion and invasion of enteropathogenic Escherichia coli to enterocyte-like cells was inhibited by high adhesiveness strain Lactobacillus reuteri JCM1081. In vitro, no significant changes of the morphology, structure and function of the HT-29 cells after being treated with Lactobacillus alone for 3 hours, especially cellular LDH level, viability and membrane permeability. Lactobacillus reuteri JCM1081 did not alter cell integrity and prevented the increase in permeability induced by enteropathogenic Escherichia coli infection. Conclusion Lactobacillus reuteri JCM1081 interact with intestinal epithelial cell receptor to competitively inhibit the adhesion and invasion of enteropathogenic Escherichia coli to HT-29 cells by incubation and enhance the integrity of the cells.