Objective To investigate the protective effects of Xuebijing(XBJ)on the injury of vascular endothelial cells(VEC)induced by lipopolysaccharide (LPS),and to study the mechanisms of the production of nitric oxide (NO)and the expressions of inducible nitric oxide sytnhase (iNOS)and signal transduction under XBJ intervention condition.Methods The cultured VEC were divided into control group, LPS (1 mg · L-1 )group, LPS (1 mg·L-1)+XBJ(25 g·L-1)group,LPS(1 mg·L-1)+pyrrolidine dithiocarbamate(PDTC,20μmol·L-1) group;XBJ and PDTC were administrated 1 h before incubation of with LPS.Western blotting method was used to detect the expressions of iNOS and NF-κB p65 protein.The level of NO in the supernatant was measured by Griess reagent.Results Comparaed with control group,the NO level and the expression levels of iNOS protein and NF-κB p65 protein in VEC in LPS group were significantly increased(P<0.01).Compared with LPS group,the NO level and the expression levels of iNOS protein and NF-κB p65 protein in VEC in LPS+XBJ group were significantly decreased (P<0.05 or P<0.01);the NO level and the expression levels of NF-κB p65 protein and iNOS protein in VEC in LPS+PDTC group were significantly decreased(P<0.05 or P<0.01).There were no significant differences of the NO levels and the expression levels of iNOS protein between LPS+XBJ group and LPS +PDTC group (P>0.05),but the expression level of NF-κB p65 protein in LPS+PDTC group was lower than that in LPS+XBJ group(P<0.05).Conclusion XBJ can inhibit the production of NO and the expression of iNOS protein in VEC;its mechanism may be related to inhibiting the activation of NF-κB to control inflammation.

Chemotherapy -induced peripheral neuropathy ( CIPN ) is one of the most common adverse effects in chemotherapy .The mechanism of CIPN has always been attracting researchers′attention.Recently,the intimate relationship between nerve growth factor and CIPN is one of the most hot topics .NGF protects neurons from damage of chemotherapy drugs through inhibiting apoptotsis and other pathway to relieve the neurotoxicity . However ,there are still many problems in the clinical application of exogenous NGF .To improve the mechanism of NGF in the development and application approach of CIPN ,treatment of chemotherapy -induced peripheral neu-rotoxicity is of great significance .

OBJECTIVE:To investigate the anti-angiogenic activity of ray cartilage glycosaminoglycans(RCG).METHODS:Primary culture of human umbilical vein endothelial cells(HUVEC)was performed,and MTT was adopted to evaluate the effects of RCG on the proliferation of HUVEC;Corneal neovascularization(CNV)was induced by sutures on Wistar rats,and CNV area was calculated to evaluate the effects of different concentration of RCG on CNV in Wistar rats.The model of mice with Lewis lung carcinoma was made to observe the effects of different concentration of RCG on tumor growth,with inhibition ratio of primary tumor measured and microvessel density(MVD)quantitated by immunohistochemical staining.RESULTS:RCG obviously inhibited the proliferation of HUVEC in vitro,with IC50 value at 62.93 mg?mL-1.As compared with normal saline group,RCG groups showed smaller areas of new blood vessels(P

Electronic Health Records ; Humans ; Medical Records ; Odontodysplasia

Hyperuricemia mice model was established with uricase inhibitor (potassium oxonate) and uric acids in serum were observed. Polydatin (5, 10, 20 mg · kg(-1)) and benzbromarone (16.7 mg · kg(-1)) were given ig for 7 d in mice. Kidney tissues were used to detect gene contents ofurate anion transporter 1 (URAT1), organic anion transporter 1 (OAT1) and organic anion transporter 3 (OAT3) by real-time-PCR. The results showed that polydatin and benzbromarone can significantly reduce uric acid in blood of hyperuricemia mice (P < 0.05), compared with the model group. URAT1, OAT1 and OAT3 contents of the kidney in hyperuricemia mice changed significantly (P < 0.05), compared with the blank group. Polydatin can significantly inhibit the changing trends in these genes induced by potassium oxonate in a dose-dependent manner, the difference was significant (P < 0.05), compared with the model group. Those indicated that polysatin could reduce the level of the serum uric acid through promoting uric acid excretion.
Animals ; Disease Models, Animal ; Glucosides ; pharmacology ; Hyperuricemia ; drug therapy ; Kidney ; drug effects ; metabolism ; Mice ; Stilbenes ; pharmacology ; Uric Acid ; blood

BACKGROUND:Lisfranc injury is rarely seen in clinical practice, with a low incidence and a high misdiagnosis rate. At present, open reduction and internal fixation is the major treatment, but there is little evidence available on the long-term fol ow-up fol owing injury and foot motor functions fol owing surgery. OBJECTIVE:To evaluate the change of foot functions after metal graft internal fixation in patients with Lisfranc injury. METHODS:Eighteen patients with Lisfranc injury were treated with internal fixation of metal grafts, such as Kirschner wire, screws and steel plate. At 6-8 weeks postoperatively, patients began to walk with crutches. After 1 year fol ow-up, the Footscan balance system and AOFAS scores were applied to evaluate the foot stability and function of patients. RESULTS AND CONCLUSION:After 1 year of internal fixation, al bone fractures were healed, the peak pressure of affected foot in the fourth metatarsal (M4) and the fifth metatarsal (M5) was significantly increased (P<0.05), and the impulse in the fifth metatarsal (M5) and mid-foot bottom (MID) was higher than the contralateral side (P<0.05). The AOFAS score of affected foot was 87.26 ± 21.13 points, the rate of excellent and good efficacy accounted for 88.9%. Internal fixation can rebuild Lisfranc complex stability, the body weight is transferred from the inside to the outside in the front foot, and the remaining pressure did not change significantly, thus the foot function is recovered satisfactorily.

Hedgehog (Hh) signaling pathway is critical during embryonic development. Recent studies have shown that Hh sig-naling plays an important role in oncogenesis and development of many malignant tumors. In particular, activation of the Hh signaling pathway is implicated in the aggressiveness and progression of gastric cancer. This review provides an overview of the research prog-ress on the role of Hh pathway in gastric cancer. Investigation of this pathway may reveal a novel target for gastric cancer therapy.

【Objective】 To investigate the incidence of β-th alassemia (β-thal) heterozygote carrying α-thalassemia (α-thal ) 1 gene in Guangdong area. 【Methods】 β-thal genes were screened by reve rse dot blotting (RDB). In β-thal DNA samples α-thal 1 genes were am plified using gap-PCR method. 【Results】43 α-thal-1 cases were identifi ed among the 500 β-thal traits. The rate is 8.6%. 【Conclusion】 In Guangd ong area the incidence of β-thal heterozygote carrying α-thal-1 gen e is 8.6%, which should be paid much attention in the genetic counselling and pr enatal diagnosis.

Objective To investigate the relationship between the content of ET-1, CGRP, NO in myocardium and cardiac function in chronic renal ischemia of rats caused by abdominal aortic banding. Methods Male wistar rats (weight 180 - 200g) were randomly divid-ed into 2 groups, operation group (n=30) and sham operation group (n=10). Abdominal aorta ligation between right and left renal ar-tery was made with silk suture in operation group, and the narrow degree of aorta was about 50% which was controlled by ligateing with a syr-inge needle (7#). The aorta was not ligated in sham operation group. After 16 weeks of operation, invasive measurement of blood pressure and cardiac function were performed, and content of ET-1, CGRP and NO in myocardium were determined. Results Compared with sham operation group, the blood pressures of rats in operation group were significantly elevated, with cardiac systolic and diastolic function de-creased and left ventricular mass index increased. After 16 weeks, compared with sham operation group, the content of ET-1 in cardiac tissue were significantly elevated in operation group (P<0.01), while the content of CGRP (P<0.01)and NO (P>0.05)were decreased. There were negative correlation between the content of ET-1 in cardiac tissue and LV +dp/dt max(r = -0.37, P<0. 05). Conclusions In the state of chronic kidney iachemia caused by abdominal aorta ligation, content of ET-1 in cardiac tissues were increased while CGRP and NO were decreased. There were negative correlation between the content of ET-1 in cardiac tissues and LV systolic function.