Objective: To prepare chicken egg yolk antibody(IgY) against Candida albicans and to study its biological properties in the interest of its further application.Methods: Ten 25 weeks old laying hens were immunized with Candida albicans.IgY was isolated from the yolks of eggs by the water dilution(WD) method.Its yield,purity,and molecular weight were measured by Bradford method and Tris-tricine gel electrophoresis,respectively.The antigenicity of IgY was determined with Western-blot method.The specific combination of IgY and Candida albicans was assayed with immunohistochemistry method.The activity of IgY was evaluated by enzyme-linked immunosorbent assay(ELISA).Results: The yield and purity of IgY were 7.9 g/L and 91.5%,respectively.It had the same molecular weight and antigenicity in comparison with IgY of chicken serum and normal chicken IgY.It could specifically combine with Candida albicans.Its titer was 1:12 000.Conclusion:The production and purity of IgY were high.Its titer was good and did not decrease under a body temperature condition after 48 h.The IgY antibody had a combination capacity of specificity for Candida albicans in vitro.

Objective To optimize the extraction process of Zushima Gel Cream.Methods Setting the content of daphnetin as an investigating index, and the amount of solvent, soak time and the extraction time as investigating factors, the orthogonal experiment was used to optimize the extraction process.Results The best extraction process was as follows:adding 30 mL water to degreasing Zushima Gel Cream;soaking for half an hour;extracting one time for two hours. The average content of daphnetin was 9.83 mg/g.Conclusion The extraction process is stable, reliable, and energy-saving. The study provided experimental evidence for the preparation process of Zushima Gel Cream.

10.3969/j.issn.2095-4344.2013.24.022

Introduced in the paper is the development and building of motor medical equipment in China,namely vehicle-mounted field hospitals,cabin hospitals,hospital ships,and rescue helicopters among others.It is pointed out that such development falls far from complete in the country,as evidenced in the poor development for airborne,and an integrated medical rescue system covering land, sea and air needs to be further enhanced in the future.

OBJECTIVE: To study the effects of Shengji Huayu Recipe (a traditional Chinese medicine compound recipe for resolving stagnation and promoting granulation) and its decomposed formulas (Huayu Recipe for resolving stagnation and Shengji Recipe for promoting granulation) on the synthesis of collagen types I and III in granulation tissue of rats in early wound healing. METHODS: Twenty-four male Sprague-Dawley (SD) rats with full-thickness skin lesion were randomized into 4 groups: Shengji Huayu Recipe-treated group, Shengji Recipe-treated group, Huayu Recipe-treated group and untreated group. Collagen types I and III in granulation tissue of the rats were tested with immunohistochemical methods and image analysis. RESULTS: On the third day of wound healing, collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group, and collagen I of the rats in Huayu Recipe-treated group was lower than that in the untreated group (P<0.05). Collagen III of the rats in the three treated groups were lower than that in the untreated group (P<0.05). On the seventh day of wound healing, Collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group (P<0.05), and collagen III of the rats in both Shengji Recipe-treated group and Huayu Recipe-treated group was higher than that in the untreated group (P<0.05). CONCLUSION: Resolving stagnation and promoting granulation therapy can promote the wound healing in rats.

This paper reviews the basic principles and calculation methods of MR specific absorption rate, discusses the clinical application of MR specific absorption rate.
Humans ; Magnetic Resonance Imaging ; Radio Waves

is of STC.Exogenous GDNF may improve the colon motor function by up-regulation of GDNF.

BACKGROUND: It has been reported that magnesium sulfate(Mg2SO4)treatment has a satisfactory effect on brain ischemia-reperfusion injury, but its effect on spinal cord ischemic injury remains unclear.OBJECTIVE: To evaluate the effects of intravenous administration of Mg2SO4 on spinal cord ischemia-reperfusion injury and further probe into its mechanism.DESIGN: Randomized controlled repeatedly measuring design based on the experimental animals.SETTING: Central research laboratory of a university hospital.MATERIALS: The experiment was conducted in the Central Research Laboratory, Medical College of Xi' an Jiaotong University from April 2003 to June 2004. Twenty-seven New Zealand white rabbits with body mass of 1.9to 2. 5 kg were included. The rabbits were randomly divided into Mg2SO4group, normal saline group and sham-operation group with 9 rabbits in each group.METHODS: The lower segment of the kidney under abdominal aorta was occluded for 30 minutes and 48-hour reperfusion was performed to establish ischemia-reperfusion model of lumbosacral segment of spinal cord. Mg2SO4group (Group A, n = 9) received Mg2SO4 at the dose of 0. 25 mL/kg per hour throughout this procedure; the same volume of saline solution was used in normal saline group(Group B, n=9) . Animals of sham-operation group (Group C, n = 9) were anesthetized and received laparotomy without aortic occlusion. The somatosensory evoked potential(SEP) was detected before ischemia, 30 minutes after ischemia, and 1, 2, 8, 16 and 24 hours after reperfusion. Motor function score was assessed in Mg2SO4 and saline groups 24 and 48 hours after reperfusion. After reperfusion for 48 hours, the animals were killed and histopathological test was performed on the spinal cord.MAIN OUTCOM MEASURES: Motor function score, SEP monitoring and spinal histopathological test.RESULTS: The latency of SEP(Nt) was markedly longer 30 minutes after ischemia in Mg2SO4 group. It was obviously recovered during the first two hours after reperfusion compared with during ischemia, but was obviously prolonged after that. Waveform disappeared 30 minutes after ischemia in normal saline group. SEP amplitudes and latencies in sham-operation group did not change remarkably during the procedures and all the animals recovered without neurological deficits. At each reperfusion time point, the recovery of SEP(N1) latency was better in Group A than that in Group B( P ＜ 0.05). The average motor function score at 24 hours and 48 hours after reperfusion was significantly higher in Group A[ (3.7 ±0.5) and(3.4 ±0.7) points] than that in GroupB [(3.0±0.7) and (2.6±0.9) points](P ＜0.05). The normal nerve cell counting of spinal cord 48 hours after reperfusion in Mg2SO4 group(23. 4 ± 3. 4) was significantly higher than that in saline group (12.3 ±3.2)(P ＜ 0.01).CONCLUSION: Intravenous Mg2SO4 administration may reduce spinal cord injury and preserve neurological function in transient spinal cord ischemia in rabbits.

OBJECTIVE: To observe the protective effects of ginkgo leaf extracts on spinal cord after ischemia-reperfusion (IR) injury in rabbits and to find out its possible mechanism. METHODS: Twenty-seven New Zealand white rabbits were randomly divided into three groups, which were sham-operation group, untreated group and ginkgo leaf extracts-treated group. The locomotor scores of hindlimbs in rabbits after 24 and 48 h of reperfusion were evaluated, and the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in ischemia spinal cord were examined. The apoptotic index (AI) of neurons in spinal cord was detected by terminal deoxyribonucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL) method. The expressions of Bcl-2 and Bax proteins were examined by streptavidin-biotin peroxidase method. RESULTS: The locomotor scores of hindlimbs in rabbits after 24 and 48 h of reperfusion in the ginkgo leaf extracts-treated group were obviously elevated as compared with those in the untreated group (P<0.05). The activity of SOD was higher and the level of MDA was lower in ischemia spinal cord in the ginkgo leaf extracts-treated group than those in the untreated group (P<0.01). The decreased neuron AI and the expressions of up-regulated Bcl-2 protein and down-regulated Bax protein were also observed in the ginkgo leaf extracts-treated group. CONCLUSION: The protective effects of the ginkgo leaf extracts against spinal cord injury induced by IR may be related to scavenging oxygen free radicals, reducing lipid peroxidation injury and inhibiting apoptosis.

Objective The aim of this study was to analyze the influence of combined therapy with L-carnitine and erythropoietin on renal anemia and nutritional status in patients treated with maintenance hemodialysis.Methods The combination of computer and manual searches were used.A Meta analysis was made regarding the effect of combined therapy with L-carnitine and erythropoietin on the improvement of renal anemia and nutritional status in patients treated with maintenance hemodialysis.The quality of the 9 studies was evaluated with the Jadad score.Statistical analysis was carried out by RevMan 5.0.Results A total of 428 patients in 9 reports were analyzed.Metaanalysis showed that there were significant improvement in hemoglobin (Hb) [WMD =9.21,95％ CI (7.69 ～10.73)] and hematocrit (Hct) [WMD =2.61,95％ CI (2.28 ～ 2.93)],albumin (ALB) [WMD =1.64,95％ CI (1.04 ～ 2.24)] and total protein (TP) [WMD =2.72,95 ％ CI (0.67 ～ 4.77)] in the treatment group compared with the control group.The level of erythropoietin dosage [WMD =-38.24,95％ CI (-76.87 ～ 0.39)] was decreased significantly.But there were no significant differences in triglyceride and serum phosphorus.Conclusion Combined therapy with L-carnitine and erythropoietin in patients treated with maintenance hemodialysis leads to significant improvement of renal anemia and nutritional status.