Objective: To prepare chicken egg yolk antibody(IgY) against Candida albicans and to study its biological properties in the interest of its further application.Methods: Ten 25 weeks old laying hens were immunized with Candida albicans.IgY was isolated from the yolks of eggs by the water dilution(WD) method.Its yield,purity,and molecular weight were measured by Bradford method and Tris-tricine gel electrophoresis,respectively.The antigenicity of IgY was determined with Western-blot method.The specific combination of IgY and Candida albicans was assayed with immunohistochemistry method.The activity of IgY was evaluated by enzyme-linked immunosorbent assay(ELISA).Results: The yield and purity of IgY were 7.9 g/L and 91.5%,respectively.It had the same molecular weight and antigenicity in comparison with IgY of chicken serum and normal chicken IgY.It could specifically combine with Candida albicans.Its titer was 1:12 000.Conclusion:The production and purity of IgY were high.Its titer was good and did not decrease under a body temperature condition after 48 h.The IgY antibody had a combination capacity of specificity for Candida albicans in vitro.

Objective To optimize the extraction process of Zushima Gel Cream.Methods Setting the content of daphnetin as an investigating index, and the amount of solvent, soak time and the extraction time as investigating factors, the orthogonal experiment was used to optimize the extraction process.Results The best extraction process was as follows:adding 30 mL water to degreasing Zushima Gel Cream;soaking for half an hour;extracting one time for two hours. The average content of daphnetin was 9.83 mg/g.Conclusion The extraction process is stable, reliable, and energy-saving. The study provided experimental evidence for the preparation process of Zushima Gel Cream.

10.3969/j.issn.2095-4344.2013.24.022

Introduced in the paper is the development and building of motor medical equipment in China,namely vehicle-mounted field hospitals,cabin hospitals,hospital ships,and rescue helicopters among others.It is pointed out that such development falls far from complete in the country,as evidenced in the poor development for airborne,and an integrated medical rescue system covering land, sea and air needs to be further enhanced in the future.

OBJECTIVE: To study the effects of Shengji Huayu Recipe (a traditional Chinese medicine compound recipe for resolving stagnation and promoting granulation) and its decomposed formulas (Huayu Recipe for resolving stagnation and Shengji Recipe for promoting granulation) on the synthesis of collagen types I and III in granulation tissue of rats in early wound healing. METHODS: Twenty-four male Sprague-Dawley (SD) rats with full-thickness skin lesion were randomized into 4 groups: Shengji Huayu Recipe-treated group, Shengji Recipe-treated group, Huayu Recipe-treated group and untreated group. Collagen types I and III in granulation tissue of the rats were tested with immunohistochemical methods and image analysis. RESULTS: On the third day of wound healing, collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group, and collagen I of the rats in Huayu Recipe-treated group was lower than that in the untreated group (P<0.05). Collagen III of the rats in the three treated groups were lower than that in the untreated group (P<0.05). On the seventh day of wound healing, Collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group (P<0.05), and collagen III of the rats in both Shengji Recipe-treated group and Huayu Recipe-treated group was higher than that in the untreated group (P<0.05). CONCLUSION: Resolving stagnation and promoting granulation therapy can promote the wound healing in rats.

Objective To establish an HPLC method for the simultaneous determination of contents of gallic acid, corilagin and ellagic acid in aqueous extract from Sanguo Decoction. Methods The analysis was carried out on an Agilent C18 column (4.6 mm × 250 mm, 5 μm), and the mobile phase was composed of acetonitrile and 0.1%phosphoric acid aqueous with gradient elution. The detection wavelength was 260 nm. The flow rate was 1.0 mL/min at column temperature of 30 ℃. Results Gallic acid, corilagin and ellagic acid were completely separated and the peak shape was good. It showed a good linearity in the concentration range of 0.174–2.61 μg, 0.04–0.60 μg, and 0.052–0.78 μg, respectively. The average recoveries of gallic acid, corilagin and ellagic acid were 99.98%, 99.81%and 100.12%, respectively. The RSD were less than 2.0%. Conclusion This method is rapid, accurate and repeatable, which can provide references for the quality control of Sanguo Decoction.

Objective To observe the effect of acupuncture, moxibustion combined with bladder function training on the neurogenic bladder.Methods 64 patients were randomly divided into the treatment group and control group,treatment group was given acupuncture,moxibustion combined with bladder function training,while the control group was treated with simple bladder function training, then comparing the bladder function changes between two groups after a month.Results In the treatment group,the bladder capacity,residual urine volume,urinate rate were respectively ( 349.4 ±13.5 ) mL, ( 98.7 ±15.3 ) mL, 92.26% and the control group respectively ( 323.4 ± 17.8)mL,(127.8 ±17.8) mL,51.85%,the difference between the two groups were statistically significant ( t =5.48,5.60,χ2 =3.97,all P<0.05).Conclusion Acupuncture,moxibustion combined with bladder function train-ing can be effective in the treatment of neurogenic bladder,while improve the quality of patient's life.

Objective To evaluate the clinical efficacy of four different methods of laparoscopic hysterectomy: laparoscopic supracervical hysterectomy(LSH),classic intrafascial supracervical hysterectomy(CISH),laparoscopic-assisted vaginal hysterectomy(LAVH),and total laparoscopic hysterectomy(TLH). Methods A retrospective analysis was carried out on 740 cases of laparoscopic hysterectomy in this hospital from January 1999 to December 2004 in respect of operating time,intraoperative blood loss,weight of removed uterus,postoperative recovery,time to normal sexual life,sexual satisfaction,and incidence of complications. Results The operations were accomplished under laparoscope in all the 740 cases.The operating time of LSH,CISH,TLH,and LAVH were 95.3?32.4 min,84.5?31.7 min,105.3?34.5 min,and 169.4?37.4 min,respectively.The time to normal sexual life of LSH,CISH,TLH,and LAVH were 30?5 d,50?9 d,35?7 d,and 54?11 d,respectively.The incidences of complications of LSH,CISH,TLH,and LAVH were 1.7%(2/120),1.3%(4/310),2.8%(7/250),and 1.7%(1/60),respectively. Conclusions Laparoscopic hysterectomy is safe and reliable.Of four methods of laparoscopic hysterectomy,each has its advantages and disadvantages,and method selection should be based on patient's own conditions.

0.05).Conclusion Aspirin plus cilostazol showed comparable antithrombotic effect as aspirin plus ticlopidine after elective coronary stenting and may serve as an alternative treatment for patients intolerable to ticlopidine.

Objective To explore the risk factors of depression in patients with functional dyspepsia.Methods From July 2014 to September 2014, a total of 295 patients was diagnosed of functional dyspepsia (FD).All of them were evaluated with the 17-item Hamilton Depression Rating Scale (HAMD-17).They were divided into two groups: FD with depression and FD without depression.Socio-demographic and clinical features were collected and analyzed.Logistic regression analysis was used to detect risk factors of depression in FD patients.Results In our studies, 20.0％ (59/295) of FD patients were diagnosed as depression.Risk factors for depression were age, disease duration, and marriage in univariate analysis, and marriage and disease duration in multivariate analysis.FD patients with moderate to severe depression had a longer duration of disease and more sessions of gastroscopy than those with mild depression.Conclusions Prevalence of depression is higher in FD patients with incomplete marriage and long disease duration, routine depression screening should be carried out for these patients.