Analysis for 6 metals (Cd, Zn, Cu, Ni, Pb, Mn) by Atomic absorption spectrophotometer were made on hair samples of healthy 135 in urban area and 130 in rural area who have not dealt with the above metals in their daily working life. Marked variations were found. Samples of urban area contained more cadmium, copper, lead and manganese than those of rural area. No sexual difference in mean value could be observed and the amount of metals in hair did not increase with age. And no age dependency was found. There was a statistically significant correlation between the concentrations of cadmium and lead in both urban and rural people; Pb=1.50 Cd + 6.69 (r = 0.213, p<0.05) in urban area. Pb=1.44 Cd + 3.67 (r = 0.327, p<0.001) in rural area.
Absorption ; Cadmium ; Copper ; Hair* ; Humans* ; Manganese ; Metals

A lot of children with congenital heart disease have an abnormal development of nervous system. A variety of biochemical and physical examination indices can be used to predict adverse outcome of neurodevelopment caused by congenital heart disease and they contribute to early intervention and improvement of neurodevelopmental disorders. In recent years, new progresses in the laboratory tests, neurological physiccal examination and perioperative management of the neurodevelopmental abnormalities caused by congenital heart disease have been made. The predictive factors of abnormal neurodevelopment caused by congenital heart disease were reviewed in this article.

This paper analyses the problems of information service to foreign students in the library of Dali University and put forwards the proper solutions.

In all enterprises of Korea employing workers more than five, employees have a duty of conducting a periodic health screening for improvement and maintaining healthful living and working conditions of employees. The health screening is performed annually in line workers and biennially in table workers under the supervision of the government. But there are some argument to the health screening programme in view of effectiveness, efficiency, reliability and etc. In order to obtain basic reference materials for increasing the efficiency of periodic health screening programme for workers and understand the attitude of health screening institutions and enterprises toward the periodic health screening, mail questionnaire survey was conducted in July 1987. The questionnaire went to 950 health screening institutions and 700 enterprises in whole land but they were returned from 254 health institutions and 187 enterprises, which were analyzed. The results were as follows: 1) The attitude of the respondents toward the necessity and benefit of the periodic health screening was showed highly positive responses in both of screening institutions and enterprises, and it was more positive in screening institutions. The attitude toward the affirmative and the contentment was showed less positive than that of the necessity and benefit. 2) The respondent recognized may problems on regard to the screening programme. The health screening institutions lie in the state of competition with other institutions for undertaking the screening programme. 3) One of major complaints from enterprises was the lack of sincerity in performing the screening programme. They wanted more practical and reliable health screening examination rather than showy one. 4) Health screening institutions and enterprises showed some mutual contradictions in the matter of screening fee, and so the screening fee should be adjusted to the appropriate cost.
Surveys and Questionnaires ; Fees and Charges ; Korea* ; Mass Screening* ; Mortuary Practice ; Organization and Administration ; Postal Service ; Surveys and Questionnaires

Objective To explore the relationship between high-risk human papillomavirus (HPV) DNA and biological behavior of cervical carcinoma. Methods Sixty-six patients of cervical carcinoma with cytological examinations and 103 patients of cervical carcinoma followed-up after surgical operation were selected for high-risk HPV DNA test with second-generation hybrid capture technique (HC2 Ⅱ). Results ①HPV DNA was positive in 62 and negative in four of 66 patients of cervical carcinoma with an overall prevalence of 94%. ②There was no significant difference in positive HPV DNA of patients with cervical carcinoma between their varied clinical stages and pathologic grades. But, HPV positivity and HPV DNA load in patients with myometrial invasion were higher than those in patients without invasion (P < 0. 05).③ HPV DNA conversed to negative in 99 of 103 patients (96%) with cervical carcinoma after surgical operation from positivity before operation. Conclusions High-risk HPV infection may correlate with angiogenesis, invasion and metastasis of cervical carcinoma and HC2 Ⅱ can be used as an effective method to detect HPV DNA.

Penile melanosis is a new disease entity which is benign and different from pigmented nevus and acral lentiginous melanoma in situ. A 20-year-old man had hyperpigmented macules on the glans penis and penile shaft for 5 years. Clinical and histologic findings were consistent with penile melanosis and giant melanosome complexes were observed ultrastructurally.
Humans ; Male ; Melanocytes* ; Melanoma ; Melanosis* ; Melanosomes ; Nevus, Pigmented ; Penis ; Young Adult

Electronic Health Records ; Humans ; Medical Records ; Odontodysplasia

ObjectiveTo study the clinical effect of locking compression plate fixation in treatment of the elders with unstable fractures of the distal radius. Methods17 cases with unstable distal radius fractures were treated by volar locking compression plate. The function of the carpal joints and the bone healing conditions were evaluated after operations. ResultsAll cases were followed-up for 7 to 16 months ( mean 12. 6 months). Union was obtained in all the patients after 11.4 weeks. The clinical outcomes were evaluated according to modified X-rays and wrist assessment. 12 cases were graded as excellent and 4 as good. 1 case were graded as poor. The overall satisfactory rate was 94. 1％. ConclusionThe unstable distal radial fractures could be effectively treated with open reduction and LCP fixation through volar approach.

Objective:To establish an HPLC method with a refractive index detector for the simultaneous determination of fructose and glucose in multiple electrolytes and invert sugar injections. Methods: A Waters XbridgeTM Amide(150 mm × 4. 6 mm,3. 5 μm) column was used with acetonitrile-water -triethylamine (75∶25∶0. 2) as the mobile phase, the column temperature was 40 ℃, the flow rate was 1. 0 ml·min-1 , the refractive index detector was used with the detector temperature of 40 ℃, and the injection volume was 50 μl. Results:A good linear relationship between the quantity of glucose or fructose and peak area was shown within the range of 99. 8-149. 7 μg(r=0. 999 7)for fructose and 100. 3-150. 4 μg(r=0. 999 2)for glucose, the average recovery of fructose and glucose was 99. 2% and 99. 5% with RSD of 0. 81% and 0. 84%(n=9), respectively. Conclusion: The method is fast and convenient with high specificity, good accuracy and promising separation, which can be used in the content determination of fructose and glucose in multiple electrolytes and invert sugar injections.

Objective To investigate the effects of down-regulation of Netrin-1 expression on cell proliferation,apoptosis and migration of a cutaneous squamous cell carcinoma(CSCC)cell line SCL-1.Methods Unspecific(control)small interference RNA(siRNA)and Netrin-1-targeting siRNA were designed and constructed.SCL-1 cells were divided into 3 groups to remain untreated(control group 1),be transfected with control siRNA(control group 2)and Netrin-l-targeting siRNA(experiment group)by using Lipofectamine 2000 respectively.After 48 hours of additional culture,flow cytometry was performed to detect the apoptosis of cells,Western blot to determine the protein expression of caspase-3 and-9,Netrin-1 and matrix metalloproteinases (MMP)in these cells,and Boyden chamber was utilized to evaluate the migration of cells.Enzyme linked immunosorbent assay(ELISA)was performed with cholecystokinin-8(CCK-8)kit to estimate the proliferation of cells at 24,48,72 and 96 hours after the transfection.Results The expression of Netrin-1 protein was significantly downregulated in SCL-1 cells in the experiment group compared with the control group 1 and 2 (0.207 ± 0.044 vs.0.741 ± 0.111 and 0.716 ± 0.109,F =31.43,P ＜ 0.05).The Netrin-1-targeting siRNA significantly downregulated the proliferation of SCL-1 cells,but induced the apoptosis in SCL-1 cells with significant differences in the percentage of early apoptotic cells and late apoptotic cells between the control group 1,2 and experiment group(11.74 ± 0.33 vs.11.55 ± 1.22 vs.24.74 ± 2.43,F=68.63,P＜ 0.01;3.82 ± 0.32 vs.4.69 ± 0.68 vs.5.33 ± 0.18,F =8.58,P ＜ 0.05).The experiment group showed a significant increase in the expression of caspase-3 and-9(1.458 ± 0.111 vs.0.398 ± 0.057 and 0.412 ± 0.049,F =183.99,P ＜ 0.01;0.967 ± 0.099 vs.0.234 ± 0.036 and 0.205 ± 0.033,F =138.11,P ＜ 0.01),but a statistical decrease in the expression of MMP-2(0.235 ± 0.038 vs.0.862 ± 0.040 and 0.892 ± 0.035,F =291.07,P ＜ 0.01)compared with the control group 1 and 2.Conclusions The down-regulation of Netrin-1 expression can suppress the proliferation and migration of SCL-1 cells,but promote the apoptosis in SCL-1 cells.