Objective To investigate the chemical constituents of Polygala fallax.Methods The chemical constituents were isolated and purified via silica gel and Sephadex LH-20 column chromatography as well as recrystallization.Their structures were determined on the basis of spectral analysis and physicochemical properties.Results Six compounds were isolated and identified as 3-O?-D-glucopyranosyl senegenic acid(Ⅰ),tenuifolin(Ⅱ),presenegenin(Ⅲ),1,7-dimethoxy-2,3-methylenedioxy xanthone(Ⅳ),sinapinic acid(Ⅴ),ferulic acid(Ⅵ).Conclusion Compounds Ⅲ—Ⅵ are isolated from P.fallax for the first time.Compound Ⅰ is a new triterpenoid saponin,named fallaxsaponin A.

Objective To elucidate the related risk factors for the cardiorenal syndrome(CRS),and to provide a reference for the prevention and treatment of CRS. Methods A retrospective study was conducted on 269 patients with chronic heart failure. The subjects were divided into two groups in accordance with estimated glomerular filtration rate(eGFR):patient group(CRS group),eGRF<60 mL/(min ! 1.73 m2)and control group,eGRF≥60 mL/(min!1.73 m2). The patients'general information as well as the related clinical lab indices and accessory examination indi-ces were collected. The risk factors for chronic heart failure and renal insufficiency were analyzed with multivariate logistic regression analysis. Re?sults The prevalence of chronic heart failure patients suffering from CRS was 26.39%. The single factor analysis showed that there were statistically significant differences between the two groups in terms of age,body weight,history of hypertension,and history of diabetes(all P<0.05). Compared to the control group,uric acid,cystatin C,serum urea nitrogen,brain natriuretic peptide,glycosylated hemoglobin,high-sensitivity C-reactive pro-tein,and left ventricular end-diastolic diameter in the CRS group were significantly higher;white blood cell count,hemoglobin,albumin,and left ventricular ejection fraction were significantly lower(all P<0.05). There was no statistically significant difference(P>0.05)between the two groups in regard to white blood cell count,glutamic-pyruvic transaminase,glutamic oxalacetic transaminase,cholesterol,triglycerides,high density lipoprotein,low density lipoprotein,troponin I,serum free triiodothyronine,serum free thyroxine,or thyroid stimulating hormone. Multivariate logis-tic regression analysis showed that older age,low body weight,history of hypertension,history of diabetes,hypoalbuminemia,superb sensitivity C-re-active protein levels,high uric acid,high cystatin C hyperlipidemia,and low ejection fraction were independently associated with the occurrence of CRS. Conclusion Older age,low body weight,history of hypertension,history of diabetes,hypoalbuminemia,high-sensitivity C-reactive protein levels,high uric acid,high cystatin C hyperlipidemia,and low ejection fraction are the high risk factors for CRS in heart failure patients and are inde-pendently associated with the occurrence of CRS. Early identification and control of these risk factors are important for the prevention and treatment of CRS.

The presence of D-amino acid in proteins is thought to be a result of racemization of L-amino acid,which may influence the space structure and the functions of proteins and the connection to the occurence of some diseases has been demonstrated.Recently,tremendous advancement in instrumental techniques has brought the rapid development to analytical methods and detecting techniques in the research on racemization of protein amino acid.The advances in the relationship between racemizadon of protein amino acid and diseases,the influence factors on racemization of protein amino acid and the analytical techniques are reviewed in this article,and the research directions of racemization of protein amino acid are also given.

OBJECTIVE: To perform a Meta-analysis of all randomized controlled trials that compared the efficacy and adverse events profile of Mabs for LA HNSCC. METHOD: Several databases were searched, including CBM, PUBMED, EMBASE, and CENTRAL. Primary outcomes included overall response rate (ORR), overall survival (OS), progression-free survival (PFS). Secondary outcomes included serious adverse events, such as grade 3-4 skin reaction, dysphagia, mucositis, and nausea/vomiting. The results were expressed as relative ratio (RR) or hazard rate (HR) with their corresponding 95% CI. RESULT: The final analysis included 10 trials. The primary analyses indicated that Mabs did not improve ORR and PFS, except OS for locoregionally advanced (LA) HNSCC [ORR 1.21, 95% CI (0.97 - 1.49); PFS 0.87, 95% CI (0.75 - 1.01); OS 0.82, 95% CI (0.71 - 0.95)]. Analysis of adverse effects demonstrated that grade 3 - 4 skin reaction ERR 1.87, 95% CI (1.11 - 3.16)] was statistically significantly associated with Mabs except dysphagia ERR 0. 95, 95% CI (0.75 - 1.19)], Mucositis ERR 1.03, 95% CI (0.67 - 1.57)], and nausea/vomiting ERR 1.15, 95% CI (0.71 - 1.86)]. CONCLUSION Anti-EGFR Mabs may be satisfactory for improving OS of LA HNSCC. During the Mabs therapy, skin reaction should be much more monitored.
Antibodies, Monoclonal ; therapeutic use ; Antineoplastic Agents ; therapeutic use ; Carcinoma, Squamous Cell ; drug therapy ; Disease-Free Survival ; ErbB Receptors ; immunology ; Head and Neck Neoplasms ; drug therapy ; Humans ; Randomized Controlled Trials as Topic ; Squamous Cell Carcinoma of Head and Neck

Animals ; Electric Stimulation ; Neurons ; pathology ; physiology ; Pain Threshold ; Rats ; Rats, Sprague-Dawley ; Red Nucleus ; pathology ; physiology

OBJECTIVETo evaluate the short-term and long-term effects on treatment of neck pain caused by cervical spondylosis with the combination of acupuncture and moxibustion with seed-size moxa cone.

METHODSOne hundred and forty-five patients of neck pain were randomly divided into an acupuncture-moxibustion group (49 cases), an acupuncture group (48 cases) and a moxibustion group (48 cases). Acupoints of Bailao (Extra), Dazhui (GV 14), Jianzhongshu (SI15) and Zhongzhu (TE 3) were adopted for all the 3 groups. Acupuncture was applied at all the acupoints with 20 min needling retention for the acupuncture group. Moxibustion with seed-size moxa cone was used with 5 cones on each point for the moxibustion group. And both acupuncture and moxibustion with seed-size moxa cone were adopted for the acupuncture-moxibustion group. The treatment was applied once every 3 days, and 10 treatments should be finished within 4 weeks. Follow-up should be carried out for 3 months. The short-term and long-term effects were evaluated with the scores of Northwick Park Pain Questionnaire (NPQ) and McGill Pain Questionnaire (MPQ) as the indices of therapeutic effect.

RESULTSThe NPQ score and MPQ score of all the 3 groups after the treating course and the 3-month follow-up were both decreased when compared with those before the treatment (all P<0. 05). The scores of NPQ and MPQ the acupuncture-moxibustion group were lower than that of the other two groups. And the difference had obvious significance (P<0. 05). High efficiency of pain relieving for cervical spondylosis could be found in all the 3 groups, which showed that short-term and long-term effects were good for all the 3 groups. And the highest curative effect could be found in acupuncture-moxibustion group.

CONCLUSIONCombination of acupuncture and moxibustion with seed-size moxa cone has reached a superior effect in short-term and long-term for neck pain caused by cervical spondylosis.

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Combined Modality Therapy ; Female ; Humans ; Male ; Middle Aged ; Moxibustion ; instrumentation ; Neck Pain ; etiology ; therapy ; Spondylosis ; complications ; Treatment Outcome ; Young Adult

Objective:To investigate the efficacy of sequential therapy of CD19 and CD22 chimeric antigen receptor T cells (CAR-T) for relapsed/refractory mediastinal B lymphoblastic lymphoma (B-LBL).Methods:One patient with relapsed/refractory mediastinal B-LBL treated with sequential therapy of CD19 and CD22 CAR-T who was admitted to Tongji Hospital of Huazhong University of Science and Technology Tongji Medical College in March 2017 was reported. At 1, 3, 6, 12 and 18 months after CAR-T therapy, the indicators of primary disease remission were monitored and the relevant literature was reviewed.Results:The patient relapsed and progressed after third-line chemotherapy, and then received sequential therapy of CD19 and CD22 CAR-T. In the course of cellular immunotherapy, the patient presented grade 1 cytokine release syndrome. After active treatment, the patient got stable condition and was discharged. The patient came to the hospital for regular review, and the mediastinal mass of the patient was dynamically followed up. After CAR-T therapy, the mediastinal mass of the patient was significantly reduced, and the patient was in continuous remission for 18 months.Conclusions:Sequential therapy of CD19 and CD22 CAR-T provides a new therapeutic approach for relapsed/refractory B-LBL. For patients with poor curative effect of conventional chemotherapy, CAR-T therapy should be actively performed as soon as possible to improve the remission rate and the long-term prognosis of patients.

OBJECTIVE: To study the distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps. METHOD: The purulent discharges were collected from sinus of 175 patients with chronic rhinosinusitis with or without nasal polyps during endoscopic sinus surgery. The results of germiculture and drug sensitivity test were analyzed. RESULT: From 175 specimens, 118 (67%) showed positive results in germiculture. Among them, 79 strains of gram positive bacteria and 39 strains of gram negative bacteria were detected. Staphylococcus epidermidis, Staphylococcus aureus and Staphylococcus haemolyticus were the most common pathogens in gram positive bacteria. The most common pathogens of gram negative bacteria were P. Aeruginosa, Enterobacter aerogenes, Enterobacter cloacae. The sensitive antibiotic on gram positive bacteria were amikacin, Daptomycin, Linezolid, vancomycin, teicoplanin, amoxicillin and clavulanate potassium, cefuroxime, respectively. The sensitive antibiotics on Gram negative bacteria were amikacin, Cefoperazone/sulbactam and imipenem, ceftazidime ceftazidime, aztreonam, levofloxacin, respectively. CONCLUSION Bacterial infection was common happened in the sinus cavity of patients with chronic rhinosinusitis with or without nasal polyps. Gram positive bacteria were the main pathogenic bacteria and gram positive bacteria and gram negative bacteria have great differences in the sensitivity of antibiotics. For patients with chronic rhinosinusitis, the using of antibiotics should depend on the drug sensitivity test.
Bacterial Infections ; complications ; drug therapy ; Gram-Negative Bacteria ; drug effects ; Gram-Positive Bacteria ; drug effects ; Humans ; Microbial Sensitivity Tests ; Nasal Polyps ; microbiology ; Rhinitis ; microbiology ; Sinusitis ; microbiology

Objective:To establish the content determination method for astragaloside in Bushen Wenfei mixtures by HPLC-ELSD. Methods:HPLCwithanAgilentZORBAXC18(150mm×4.6mm,5μm)columnwasused. Methanol-water(75∶25)wasusedasthe mobile phase and the flow rate was 0. 8 ml·min-1 . The drift tube temperature was 80℃ and the gas flow rate was 2. 5 ml·min-1 . Results: The linear range of astragaloside was 0.297-4.944 μg (r =0.999 7). The average recovery was 98.13% with RSD of 1. 27%(n=9). Conclusion:The method is with sufficient accuracy, stability and repeatability, and can be used in determination of Bushen Wenfei mixtures.

Streptozotocin-induced diabetic rats were treated with GSH for 12 weeks.The results showed that GSH significantly improved the expressions of NF-KB and inducible nitrie oxide synthase and ameliorated the myocardial tissue injury.