Objective To investigate the effects of P27Kip-1 on proliferation of airway smooth musde and airway remodeling in asthmatic rats.Methodes 30 healthy,male SD rats were randomly and equally divided into three groups :control group(C),asthmatic 4 weeks group(A1),asthmatic 6 weeks group(A2).A rat asthma model was developed by the sensitized with ovalbumin and Al(OH)3,and repeatedly exposed to aerosolized ovalbumin.After the last challenge,The animals were evaluated for AHR,the airway wall thickness(Wat /Pbm)and the bronchial smooth muscle thickness(Wam/Pbm)were measured by image analysis system.The expression of P27Kip-1 and PCNA was determined by immunohis-tochemical staining.Results(1).The expression of P27Kip-1 protein in two asthmatic groups was significantly lower than that of the control group,respectively(P

Objective To observe the expression and correlation of Twist,p53 and E-cadherin mRNA in breast cancer.Methods Thirty breast cancer tissue and adjacent noncancerous tissue in patients underwent modified radical surgery,and the normal breast tissue in 10 patients with benign breast lesions,respectively,were detected the expression of Twist,p53 and E-cadherin mRNA by RT-PCR and the relationship with clinicl pathological factors and their interrelationships.Results There was significant correlation between the expression of Twist mRNA and the degree of differentiation[high and middle differentiation 93.33％(14/15),1ow differentiation 53.33％(8/15)],lymphatic invasion[yes 93.75％(15/16),no 50.00％(7/14)]and TNM staging[Ⅰ-Ⅱ?stage 46.15％(6/13),III-Ⅳ?stage 94.12％(16/17)]in breast cancer tissue(P＜0.05),while there was no obvious correlation between the expression of Twist mRNA and the age,tumor diameter,adventitial infiltration,estrogen receptor,progesterone receptor(P＞0.05).The expression of Twist and p53 mRNA in breast cancer tissue were significantly higher than those in adjacent noncancerous tissue and normal breast tissue[(112.36±12.53)％ vs.(101.37±9.12)％ and(98.03±9.04)％,(101.73±13.32)％ vs.(91.43±7.43)％ and(90.83±6.58)％,P＜0.01],while the expression of E-cadherin mRNA in breast cancer tissue was significantly lower than that in adjacent noncancerous tissue and normal breast tissue[(55.43±12.49)％ vs.(69.39±5.63)％ and(72.73±7.93)％,P＜0.01],there was no significant difference between adjacent noncancerous tissue and normal breast tissue(P＞0.05).The expression of Twist mRNA in breast cancer tissue was positively correlated to the expression of p53 mRNA(r=0.526,P=0.004),and negatively correlated to the expression of E-cadherin mRNA(r=-0.500,P=0.000).Conclusions Twist and p53 in breast cancer tissue show higher expression,while E-cadherin shows lower expression.Twist can promote invasion,metastasis of breast cancer by up expression of p53 and down expression of E-cadherin,and it plays an important role.

[Objective]To investigate the etioloty of the early nerve root lesion(RNRL) after collagenase chemonucleolyoio(CC) and its prevention and treatment.[Method]Twelve-hundred and eighty case of lumbar disc herniation undergoint cc were reviewed and analyzed.From January,2000 to December,2004 with follow-up time of 4~30 months,in which 12 cases(0.94%)had obvious ENRL after CC.Eighteen rabbits were randomly divided into three groups of six each:Group A,posterior longitudinal ligament intepated+intradisca injection;group B,parterior longitudinal ligamen+booteen+intradiscal injection,and group C,extradiscae injection.The disc and the nerve roots at the inject side were removed,fixed and examined by microscope,and the changes of the discs end nerve roots were analyzed in different inject side and different time after CC.[Result]As the collagenase dose was large in groups of intradiscal+intradiscal injections case of ENRL in this group were more than that in other groups.In the 12 ENRL cases,ten cases of ENRL appeared in about 7 days after CC,8 cases without nerve root compression and all cases were improved in different degree,but no one reeovered completely at follow-up with excellent and good rate of efficiency in the cases operated in 3 days than that in cases operated after 8 days.In the experiment rabbits,there lowed obvious lyeis of nucleus.Pulpus in group A and group B,with severe damage of nerve roots in group B,while in group C,there was no obvious lipis of nucleuss pulpus and damage of nerve roots[Conclusion](1)As the larger collagenas dose in the cases with intradiscal and extradiscal injections can raise.The incidence of ENRL,it is suggested that CC used in cases with broken posterior longitudenal ligament of and brous anmulus and,with nucleus pulpuses herniated into spinal canal,CC should be prudent to apply.(2)The prognoseo of the ENRL after CC is poor and is related to operation time.(3)The incidence of ENRL after CC can be lowered with absolute bed rest and changing body position after CC.

Object To study the chemical constituents of the dried root of Fordia cauliflora Hemsl which is indigenous to Guangxi and commonly used in traditional medicine of Zhuang and Yao nationalities Methods The constituents were isolated with column chromatography and their structures were elucidated on the basis of physicochemical data and spectral analysis Results The three constituents were isolated and identified as 2 [3 methoxyphenyl] 4H furo[2,3 h] 1 benzoyran 4 one (Ⅲ), 3 methoxy 2 [3 hydroxyphenyl] 4H furo [2, 3 h] 1 benzopyran 4 one (Ⅳ) and pinnatin (Ⅴ) Conclusion The all of them are isolated from this plant for the first time Compound Ⅲ,Ⅳ are new and tentatively named as cauliflorin A and cauliflorin B

AIM: To improve the acute cumulative death rates method (ACD method) in animal by oral administration. METHODS: A mathematic model was established to evaluate the dose-toxic effect relationship by twice oral administration and the experimental steps were improved too. The methodological quality was explored using the simulation data from computer program and the real experimental date from the reference paper. RESULTS: The results showed that the experimental data could be fitted to its theoretical data from LD_5/2 to LD_ 95/2. Concentration-time curve after po ordinary powder of Semen Strychni in mice were fitted to a one-compartment with T_ 1/2(ka)= 1.136 h,T_ 1/2(ke)= 7.100 h,and T_ max= 3.576 h. CONCLUSION: The improved ACD method can be used in the pharmacokinetics of TCM by oral administration.

Objective To evaluate and compare the diagnostic value of digital mammography-guided biopsy with mammography for early-stage breast cancer. Methods 68 of 156 foci(from 148 cases) with diameter≤2.0 cm from 2850 mammographic cases during Nov.2001 and Sep.2002,were urdestaken mammography-guided biopsy and then compared the coherence of the biopsy and mammographic results with the pathology. Results The obviously accuracy of mammography-guided biopsy is obviously superior to the mammography(P

Both the concept and characteristics of materials circulation management in hospital are expounded.In order to implement materials circulation management in hospital,it is proposed that not only the outside resources should be utilized rationally,but the control of internal materials circulation links should be strengthened,and specially the purchasing and storage management should be highlighted.According to the experience of materials circulation management,it is very necessary to carry on internal materials circulation management in hospital.Any improvement in any link will play a driving role in the improvement of the management level of the hospital.At meantime,the scientific system of internal logistics management should be set up and improved constantly in hospital.

OBJECTIVE: To study the change of consumption of antibacterial agents especially the ceftazidime and its effect on drug resistance of Pseudomonas aeruginosa (PA). METHODS: The consumption of the top ten antimicrobial agents in terms of cases/times of medication from 2005 to 2006 in a hospital and its correlation with the drug resistance of Pseudomonas aeruginosa were analyzed. RESULTS: A linear correlation was noted between the consumption of ceftazidime and resistance of Pseudomonas aeruginosa to ceftazidime and another seven antimicrobial agents in the next quarter (P

Objective To study testicular microlithiasis(TM)on scrotal ultrasound findings in re-lation to the incidence of varicocele in males with scrotal symptoms.Methods Ultrasound finding of 8351 cases aged 40 days to 88 years with scrotal symptoms were reviewed.Results TM was found in 170 pa-tients(2.04%)and varicocele Was found in 1449 patients(17.35%)of symptomatic individuals.Varicocele Was found in TM and non-TM were 15.88%(27/170)and 17.38%(1422/8181)respectively,statistical anal-ysis revealed that there was no close relationship between TM and varicocele(x2=0.26,P>0.05)in symp-tomatic population. Conclusion TM is a comnlofl disease and has no direct correlation with varicocele.