No abstract available.

Face ; Humans ; Reconstructive Surgical Procedures ; methods ; Skin Neoplasms ; surgery ; Skin Transplantation ; methods ; Surgical Flaps

Asian Continental Ancestry Group ; Humans ; Language ; Medicine, Chinese Traditional ; Translations

No abstract available.
Child* ; Fever* ; Humans

No abstract available.
Primary Health Care*

AIM: To investigate the effects of c-fos antisense oligoneuleotide and p21 genetic transfection on the intimal proliferation of venous autografts. METHODS: The external jugule veins were autografted into common carotid arteries in the same side in 20 New Zealand rabbits, which were divided evenly into experimental and control group randomly. The transplanted veins of experimental group were immersed in the adenovirus - mediated p21 gene solution for 15 minutes just before anastomosis and coated with c-fos antisense oligoneucleotide glue gel just after anastomosis, while the control was only treated with empty vector. The transplanted vascular sample were taken at 2 weeks after operation. The intimal thickness (IT), degree of restenosis (DR), expression of proliferating cell nuclear antigen (PCNA), quantity of VSMC were determined by immunohischemistry. RESULTS: The IT , DR and expression of PCNA, VSMC were decreased, compared to control group. CONCLUSION: Transfection of c-fos antisense oligoneuleotide and p21 gene inhibits the intimal proliferation of venous antografs. [

Objective To study the chemical constituents from alcohol extract and water extract in Lepidozia vitrea in order to find out their bioactive compounds.Methods The chemical constituents were isolated by various column chromatographic methods and the structures of the compounds were elucidated by spectral analysis.Results Six compounds were obtained and their structures were identified as phytol (Ⅰ),7-hydroxycalamenene(Ⅱ),daphnetin(Ⅲ),and daucosterol(Ⅳ) from the alcohol extract,D-glycero-D-galacto-heptitol(Ⅴ) and D-erythro-L-galacto-octitol(Ⅵ) from the water extract.Conclusion Compounds Ⅰ-Ⅵ are isolated from this plant for the first time.

Objective:To construct the expression vector pSuper.Hsp90?/pSuper for further research of the Hsp90?.Methods: In accordance with the Hsp90? sequence in gene bank,three sequences respectively targeting Hsp90? were cloned into the RNA expression vector pSuper.Results:It was confirmed by enzyme digestion,and electrophoresis that the construction of the recombinant expression vector pSuper-Hsp90?1,pSuper-Hsp90?2 and pSuper-Hsp90?3 was successfu1.pSuper-Hsp90?1 and pSuper-Hsp90?2 were successfu1 by sequencing.The expression of Hsp90? mRNA in HEK293 cells with pSuper-Hsp90?1,pSuper-Hsp90?2 and pSuper-Hsp90?3 decreased,and pSuper-Hsp90?1 notably decreased.Conclusion:The recombinant plasmid against Hsp90? RNAi can be successfully constructed in vitro,and pSuper-Hsp90?1 is the possible optimal target.

Objective:To observe the effect of oral health through periodontal sealing and oral hygiene education on the peri- odontal disease patients undergoing fixing orthodontics. Methods:81 periodontal disease subjects who had been cured or con- troled effectively were divided randomly into two groups.The experimental group:oral hygiene education and sealing one time every month. The control group:underwent oral hygiene education. Dental plaque index (PLI),gingival index (GI) and mobility (M) were examined at four times:before orthordontics,at 4 month.8 month and the end of treatment. X-ray picture before treatment and after treatment were contrasted.Datas were analysed with statistics. Results:Slatistics analysis dissovered that the PLI and the GI was remarkably different between the experimental group and the control group (P

Objective:To investigate the expression and the effects of CD40/CD40L in lung tissue of rabbits with hemorrhagic shock.Methods:18 healthy rabbits were randomly divided into control group(group A),hemorrhagic shock 1 hour group(group B) and hemorrhagic shock 2 hours group(group C),with 6 rabbits in each group.Except the rabbits in the control group,rest rabbits were made into hemorrhagic shock models with lung injury according to Wiggers modified method.TNF-?、IL-10 in plasma were measured by ELISA.Expressions of CD40 and CD40L in lung tissue were measured by immunohistohemistry.Water quantity and pathological change in the lung were observed.Results:The lung of group B and C displayed obvious injury,water quantity increased(P