Objective To detect neurotransmitters function in the brain and the brain functional index of outpatients with methadone maintenance treatment,and understand the change features of brain function.Methods Collect brain electrical power signals were collected from 58 outpatients with methadone maintenance treatment (MMT) and 44 healthy adults,and the correlations between the power of the neurotransmitters and the brain functional status were analyzed.Results (1) In MMT group,all neurotransmitters were lower generally than those in the control group,and the differences of GABA ((17.73±3.54) μV2 vs.(121.48±44.64) iμV2,P＜0.01),Glu ((42.18±12.84) μV2 vs.(105.31 ± 34.95) μV2,P＜0.05) were significant.(2) The relative powers of GABA ((17.10±51.72) μV2 vs.(78.67± 10.93) μV2,P＜0.001) and Glu((30.48±21.61) μV2 vs.(69.23±42.26) μV2,P＜0.001) reduced significantly,while 5-HT((297.18±31.54) μ V2 vs.(280.18±31.54) μV2,P＜0.01),ACh ((235.08±37.72) μV2 vs.(217.23±40.60)μV2,P＜0.05),NE((164.11±33.05) μV2 vs (146.39±30.80) μV2,P ＜0.01),DA((98.87±22.48) μV2 vs.(91.49±21.04)μV2,P＜0.05) rised significantly in MMT group.(3)The global power was lower((1012.01± 195.09)μV2 Vs.(1775.94±458.99) μV2,P＜0.01),and the excitement-inhibition index(2.19± 1.46 vs.0.99±0.47,P＜0.001) and the relative entropy((89.45±9.71) ％ vs.(75.48±9.97) ％,P ＜0.01)were higher than those in the controls.(4) In MMT group,there were positive correlation between all neurotransmitters and global power,as well as that between Glu and excitement-inhibition index (r=0.264,P＜0.05),NE and Vasomotor index(r=0.269,P＜0.05),but negative correlation between relative entropy and 5-HT(r=-0.276,P＜0.05) as well as DA(r=-0.375,P＜0.01)significantly.Conclusion The brain function of outpatients with MMT are lower than the normal significantly,and there are clearly imbalance between the excitement and inhibition among the brain neurotransmitters.

0.05).Conclusion Aspirin plus cilostazol showed comparable antithrombotic effect as aspirin plus ticlopidine after elective coronary stenting and may serve as an alternative treatment for patients intolerable to ticlopidine.

Objective To investigate the clinical effect of butylphthalide combined with naloxone in the treatment of elderly patients with cerebral hemorrhage.Methods A total of one hundred and thirty elderly patients with cerebral hemorrhage were selected in North China University of Science and Technology Affiliated Hospital from December 2012 to December 2014,and were randomly divided into two groups,each group 65 cases.Naloxone was used in the control group,while the observation group adopted butylphthalide combined with naloxone in its treatment.The curative effects were compared between two groups.Results After treatment,homocysteine,C reactive protein,hypoxia inducible factor,neurological function defect score were all significantly decreased in two groups (P<0.05).Homocysteine,C reactive protein,hypoxia inducible factor and neurological function defect score in the observation group were much lower than those of the control group ((10.2±1.3) μmol/L vs.(14.0±1.8) μmol/L,(3.9±1.1) mg/L vs.(6.9±1.4) mg/L,(51.6±10.3) pg/ml vs.(82.5±17.6) pg/ml,(3.7±1.0) points vs.(4.4±0.9) points,t=7.134,10.692,9.078,4.892,P<0.05).The total effective rate in the observation group was significantly higher than that of the control group (98.5% vs.89.2%,χ2=4.795,P<0.05).Compared the complications in two groups,the differences were not statistically significant (P>0.05).Conclusion Butylphthalide combined with naloxone is effective in the treatment of elderly patients with cerebral hemorrhage,which can significantly improve their clinical signs and neurological function.

The present paper makes a tentative study on the significance and the approaches of the undergraduates' ideological education in the present new situation,stressing that currently universities and colleges must strengthen the construction of the CPC organizations at universities and boost the construction of the education of talented people in an all-round way with the thought of "3 represents" as guidelines,the strengthening of the construction of the CYLC as foundation and the conducting of the ideological education of the construction of the CPC as means.

Purpose　To study the effect of heparin on expression of type collagen Ⅰ,ⅢmRNA in cultured fetal rat lung fibroblast (LFb). Methods　By using Northern blot method and immage analysis,the expression of collagenⅠ, Ⅲ mRNA were quantitatively analysed in the fibroblasts treated with different concentration heparin (0.1 mg/L, 1 mg/L, 10 mg/L, 100 mg/L)separately. Results　Heparin treatment at 0.1 mg/L,1 mg/L, 10 mg/L and did not influence the expression of collagen Ⅰ,Ⅲ mRNA，but the expression of collagen Ⅰ,Ⅲ mRNA were inhibited at 100 mg/L. Conclusion　Expression of collagen Ⅰ, ⅢmRNA is related to the concentration of heparin.

Objective To discuss the neuropsychological mechanism of executive control for methadone maintenance patients(MMP).Methods Using the event-related potentials (ERPs) technique to reveal the time course of information processes with high temporal resolution,and exam the function of attention monitoring and response inhibition in heroin-dependent MMP.Results (l) The behavioral results: there were significant withinsubjects Stroop interference effects for the MMP and control groups (reaction time:control group' s congruent condition(766.57±75.64) ms,incongruent condition(879.52±62.31) ms,MMP group' s congruent condition (821.89±64.44) ms,incongruent condition (906.29 ± 69.46) ms,P＜ 0.001 ; error rate: control group' s congruent condition (4.15± 2.92) ％,incongruent condition (8.70 ± 6.12) ％,P＜ 0.001,MMP group' s congruent condition (12.07 ±10.80) ％,incongruent condition (16.60±12.43)％,P＜0.01).(2)ERPs data showed that MMP demonstrated significantly smaller incongruent-N2/N450/SP amplitudes than controls,and all disappearing incongruent effects in N2,N450 and SP,comparing statistically significant incongruent effects for controls in N2,N450 and SP.In MMP group,the amplitudes of N2 and N450 incongruent condition (N2 (1.40± 2.91) μV、N450 (1.29 ± 0.55) μV) were bigger than congruent condition amplitudes(N2(0.77±3.61) μV 、N450(0.83± 1.07) μV,P＜0.05),but the amplitude of SP (0.37±3.58)μV was smaller than congruent condition(1.53±3.05) μV,P＜0.001 ;in control group,the amplitudes of N2 and N450 incongruent condition((-0.30±3.45) μV,N450(1.77± 1.55)tμV) were smaller than congruent condition (N2(1.10±3.64) μV,P＜0.001； N450(2.37±2.12) μV,P＜0.05),the amplitude of SP ((1.93±1.65) μV) was bigger than congruent condition((0.98±2.02)μV,P＜0.01).Conclusion There are impaired executive control functions during the conflict monitoring process and the conflict resolution process for MMP.These results provide the neural electrophysiological evidence to explain relapse behaviors in methadone maintenance patients.

Objective To investigate the influence of impulsivity on the functions of the conflict monitoring and the conflict resolution process for heroin addicts.Methods 80 heroin addicts were drawn randomly using simple random sampling method and finished Opioid Addiction Severity Inventory ( OASI ) and Barratt Impulsivity Scale ( BIS-11) .High impulsivity group( 7 females of 22) and low impulsivity group (8 females of 21) were divided according to 27%of the total BIS-11score followed the grouping method in psychometrics.Then the event-related potentials (ERPs) technique with the classical color-word Stroop task was used to reveal the influence of impulsivity on the executive function for heroin addicts.Results ( 1) The scores of motor impulsivity(23.00±1.73) and no-plan impulsivity(27.77±3.22) were higher in high im-pulsivity group than those in low impulsivity group(19.31±2.80,23.38±3.59)(P<0.01);and there was posi-tive correlation between impulsivity and addiction severity( r=0.415, P<0.05).(2) The behavioral data showed significant Stroop interference effects in both groups(P<0.01).(3) ERPs data showed that there were significant incongruent-N450 and SP effects in the low impulsivity group,whereas incongruent N450 and SP effects disappeared in high impulsivity group ( Low impulsivity group incongruent vs congruent condition N450:(2.82±3.09)μV vs (4.51±2.77)μV, P<0.05; SP:(3.54±1.25)μV vs (2.84±1.03)μV, P<0.05;High impulsivity group incongruent vs congruent condition N450:(4.98±4.10)μV vs (3.39±3.31)μV, P<0.05;SP:(3.43±3.84)μV vs (4.66±4.53)μV, P<0.05).Conclusion The brain time-interval change of executive function such as the conflict monitoring and the conflict resolution process is influenced by the im-pulsivity levels of heroin addicts.

Objective To investigate the difficulties of building channel in ultrasound-guided percutaneous nephrolithotomy (PCNL).Methods Surgical processes of 45 patients underwent PCNL were recorded.The difficulties and causes in building channel were analyzed.Results In the process of building channel in PCNL,60 cases of difficulties happened,41.7％ (25/60) because of depth inaccuracy of puncture and dilation,18.3％(11/60) because of angle deviation of tract dilation,15.0％(9/60) result from nondilated collecting system,13.3％(8/60) because of lacking experience in ultrasound observation.Conclusions The most often encountered difficulties is depth inaccuracy of puncture and dilation in building channel,next is angle deviation of tract dilation.To improve technique detailes is the key to gain success.

Objective To evaluate the effect of ginsenoside-Rg3 on cellular immune function of nasopharyngeal carcinoma patients with radiotherapy. Methods 50 nasopharyngeal carcinoma patients with radiotherapy were ran-domly divided into Rg3 group ( n=25 ) and control group ( n=25 ) . After the radiotherapy, the peripheral blood lymphocy of each group were collected. MTT method was used to detect lymphocyte proliferation. Lymphocyte sub-group was detected by Flow Cytometry/financal capacity model. IgG,IgM and Th1/Th2 cytokines was detected by ELISA. Results Ginsenoside Rg3 could promote the proliferation of lymphocytes in patients with nasopharyngeal carcinoma radiotherapy,upregulation of CD4 +,CD4 +/CD8 +,IgG,IgM,IL-2,downregulation of CD8 +,IL-6,and showed dose dependent. Conclusion Rg3 ginsenosides can significantly enhance the peripheral blood lymphocyte immune function of nasopharyngeal carcinoma patients with radiotherapy. Rg3 ginsenosides may be a potential im-mune immunosuppression caused by nasopharyngeal carcinoma radiotherapy enhancer antagonism.

OBJECTIVE:To establish a method for the simultaneous determination of uracil,hypoxanthine,xanthine,uridine and adenosine in Eisenia foetida. METHODS:HPLC was performed on the column of Venusil XBP C18 with mobile phase of 50%methanol-0.01 mol/L potassium dihydrogen phosphate solution(gradient elution)at a flow rate of 1.0 ml/min. The detection wave-length was 254 nm with a column temperature at 30 ℃,and the injection volume was 10 μl. RESULTS:The linear range was 0.05-1.60 μg for uracil(r=0.9991),0.05-1.60 μg for hypoxanthine(r=0.9993),0.002-0.010 μg for xanthine(r=0.9991), 0.0075-0.24 μg for uridine(r=0.9999)and 0.0250-0.80 μg for adenosine(r=0.9990);RSDs of precision,stability and accuracy tests were lower than 3%;recoveries were 98.30%-100.76%(RSD=0.8%,n=9),98.68%-100.96%(RSD=0.8%,n=9), 95.16%-97.67%(RSD=0.9%,n=9),96.15%-99.57%(RSD=1.5%,n=9)and 96.39%-101.93%(RSD=1.8%,n=9),respective-ly. CONCLUSIONS:The method is simple and stable with good reproducibility,and can be used for the simultaneous determina-tion of uracil,hypoxanthine,xanthine,uridine and adenosine in E. foetida.