Objective: To prepare chicken egg yolk antibody(IgY) against Candida albicans and to study its biological properties in the interest of its further application.Methods: Ten 25 weeks old laying hens were immunized with Candida albicans.IgY was isolated from the yolks of eggs by the water dilution(WD) method.Its yield,purity,and molecular weight were measured by Bradford method and Tris-tricine gel electrophoresis,respectively.The antigenicity of IgY was determined with Western-blot method.The specific combination of IgY and Candida albicans was assayed with immunohistochemistry method.The activity of IgY was evaluated by enzyme-linked immunosorbent assay(ELISA).Results: The yield and purity of IgY were 7.9 g/L and 91.5%,respectively.It had the same molecular weight and antigenicity in comparison with IgY of chicken serum and normal chicken IgY.It could specifically combine with Candida albicans.Its titer was 1:12 000.Conclusion:The production and purity of IgY were high.Its titer was good and did not decrease under a body temperature condition after 48 h.The IgY antibody had a combination capacity of specificity for Candida albicans in vitro.

Objective To optimize the extraction process of Zushima Gel Cream.Methods Setting the content of daphnetin as an investigating index, and the amount of solvent, soak time and the extraction time as investigating factors, the orthogonal experiment was used to optimize the extraction process.Results The best extraction process was as follows:adding 30 mL water to degreasing Zushima Gel Cream;soaking for half an hour;extracting one time for two hours. The average content of daphnetin was 9.83 mg/g.Conclusion The extraction process is stable, reliable, and energy-saving. The study provided experimental evidence for the preparation process of Zushima Gel Cream.

10.3969/j.issn.2095-4344.2013.24.022

Introduced in the paper is the development and building of motor medical equipment in China,namely vehicle-mounted field hospitals,cabin hospitals,hospital ships,and rescue helicopters among others.It is pointed out that such development falls far from complete in the country,as evidenced in the poor development for airborne,and an integrated medical rescue system covering land, sea and air needs to be further enhanced in the future.

OBJECTIVE: To study the effects of Shengji Huayu Recipe (a traditional Chinese medicine compound recipe for resolving stagnation and promoting granulation) and its decomposed formulas (Huayu Recipe for resolving stagnation and Shengji Recipe for promoting granulation) on the synthesis of collagen types I and III in granulation tissue of rats in early wound healing. METHODS: Twenty-four male Sprague-Dawley (SD) rats with full-thickness skin lesion were randomized into 4 groups: Shengji Huayu Recipe-treated group, Shengji Recipe-treated group, Huayu Recipe-treated group and untreated group. Collagen types I and III in granulation tissue of the rats were tested with immunohistochemical methods and image analysis. RESULTS: On the third day of wound healing, collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group, and collagen I of the rats in Huayu Recipe-treated group was lower than that in the untreated group (P<0.05). Collagen III of the rats in the three treated groups were lower than that in the untreated group (P<0.05). On the seventh day of wound healing, Collagen I of the rats in both Shengji Huayu Recipe-treated group and Shengji Recipe-treated group was higher than that in the untreated group (P<0.05), and collagen III of the rats in both Shengji Recipe-treated group and Huayu Recipe-treated group was higher than that in the untreated group (P<0.05). CONCLUSION: Resolving stagnation and promoting granulation therapy can promote the wound healing in rats.

Objective:To investigate the interfering effect of siRNA on the expression of VEGF in Hela cells.Methods:Three pairs of siRNA were designed according to the 1-5 extrons sequence of VEGF and synthesized by T7 RNA ploymerase transcription in vitro. To evaluate the inhibition activity of siRNA, Hela cells were transfected via siPORT Lipid. The interfering effect of siRNAs in hRPE cells was examined by semi-quantitative RT-PCR and immunofluorescence technique.Results:The results showed that the three pairs of siRNA could effectively inhibit gene expression of VEGF in Hela cells with the rates of 88.7%, 94.2% and 80.3%. But the 1-base mismatched siRNA and ssRNA(+) didn′t show significant interfering effect. Furthermore, it was found that siRNAs had a dose dependent inhibitory effect(5-10 pmol).Conclusion:The siRNAs synthesized by T7 RNA ploymerase in vitro could effectively and specifically interfere the expression of VEGF in Hela cells, providing a novel approach for gene therapy of tumor.

is of STC.Exogenous GDNF may improve the colon motor function by up-regulation of GDNF.

OBJECTIVE:To evaluate the utilization status and trend of anti-vomiting drugs in our hospital. METHODS: The anti-vomiting drugs used in our hospital during the 2001～2006 were analyzed statistically in respect of drug kinds, dosage forms, consumption sum, DDDs etc. RESULTS: Both the drug kinds and the consumption sum of anti-vomiting drugs increased year by year in our hospital over 6 years, dominating the first place was 5-HT3 antagonists, of which, Ondansetron topped the list in consumption sum for five years and its DDDs ranked at the first place for 4 years. Domestic injections took the lead among all the anti-vomiting drugs. CONCLUSION: Consumption quantity of 5-HT3 antagonists has a rapid increase and which occupy a dominate place in our hospital.

Objective:To investigate microRNA-29b ( miR-29b) expression in cerebral cortex , spinal cord, fore limb muscle, and serum of SOD1-G93A amyotrophic lateral sclerosis ( ALS) mice, and to identify the biomarker and to assess diagnostic values for ALS .Methods:Cerebral cortex , spinal cord , fore limb muscle and serum from 16 SOD1-G93 A ALS mice and 16 wild-type mice were taken and then microRNA extracted , detecting the expression of miR-29 b by real-time quantitative polymerase chain re-action ( RT-qPCR ) .The diagnostic performance of miR-29b for ALS was estimated by the receiver operating characteristic ( ROC ) curve . Results: The results from the validation indicated that the differences in miR-29b between the cerebral cortex of SOD1-G93A ALS and the healthy control subjects were statistically significant (P=0.001).Meanwhile, the expressions 8, 12, and 16 weeks later were higher than those of the controls ( ALS vs.Control: 8 weeks, P=0.044; 12 weeks, P=0.018; 16 weeks, P=0.045).When the relative expression level of miR-29b was used to diagnose ALS in SOD1-G93A ALS mice, the area under the ROC (area under the curve, AUC) was 0.885, if the diagnostic threshold was set at 0.185 6, the sensitivity and specificity were 92.9%and 71.4%.Conclusion:MiR-29 b may act as medical monitoring indices of ALS in early time .

This paper reviews the basic principles and calculation methods of MR specific absorption rate, discusses the clinical application of MR specific absorption rate.
Humans ; Magnetic Resonance Imaging ; Radio Waves