Objective To investigate somatostatin effect of on curative effect of laparoscopic operation in treatment of adhesive intestinal obstruction.Methods 80 patients with adhesive intestinal obstruction from January 2013 to May 2014, were randomly divided into control group and observation group, 40 cases in each group; the control group: treated with laparoscopic operation at the same time, from the patients admitted to the hospital after the foundation treatment; the observation group: somatostatin injection based on the treatment in control group.To observe the therapeutic effect of two groups, gastrointestinal decompression drainage, anal exhaust time, hospitalization time, the rate of conversion to surgical operation, postoperative analgesia, postoperative complications, follow-up treatment within 1 years after the recurrence rate, and application of functional EORTC life quality evaluation of the quality of life of two groups of patients with postoperative score.Results After treatment, the total effective rate of control group was 85%, the total efficiency of observation group was 92.50%, but the difference was not statistically significant (χ2 =2.635).After treatment, patients in observation group,the amount of gastrointestinal decompression, intestinal peristalsis recovery time, anal exhaust time, hospitalization time surgical operation and transfer rate were significantly lower than control group (P<0.01).By measuring the quality of life of EORTC scores between two groups, the observation group was significantly higher than the control group, there was significant difference ( P <0.01 ) .The success rate of the operation of the two groups was 100%, the observation group recurrence after 1 years of follow-up, the number of analgesic were significantly lower than the control group, there were significant differences.Complications in the observation group compared with the control group, the difference was not statistically significant (χ2 =2.813).Conclusion Laparoscopic operation in treatment of adhesive intestinal obstruction, given somatostatin can improve the clinical curative effect, shorten the recovery time of patients, reduce the recurrence rate, improve the survival of treatment.

Background and purpose:Studies have shown that DOC-2 could work as a potential tumor suppressor geue,and the role of DOC-2 in terms of the inhibition of cell growth and its mechanism remain unknown.Our paper is to investigate the effect and mechanism of DOC-2 expression on the tumorigenesis viability of ovarian cancer cell line HO-8910 from the aspects of clone efficiency,cell cycle and animal model test.Methods:Three cell lines were used including HO-8910,8910-P93(transfected with DOC-2 gene) and 8910-pcDNA3.1(transfected with the vector pcDNA3.1).Firstly,soft agar method was used to measure the clone efficiency.The cell cycle were analyzed by flow cytometer.The tumorigenesis viability was compared by athymic mouse test.Results:After being transfected with DOC-2 gene,the clone efficiency of 8910-P93 was markedly reduced.There was no difference between the 8910-pcDNA3.1 and HO-8910.G1 and G2 arrest were observed for 8910-P93.The athymic mouse test showed that the neoplasm derived from 8910-P93 was much smaller than that in the controls.Conclusions:DOC-2 could iniibit the tumorigenesis viability of human ovarian cancer line HO-8910.

Objective To evaluate the effects of the two methods of propaganda and education, conventional training method and that combined with hand contamination survey method, on the medical and nursing staffs' hand hygiene compliance. Methods From October 2017 to April 2018, all medical and nursing staffs in 6 Departments:General Surgery Ⅰ, General Surgery Ⅱ, Orthopedics Ⅰ, Orthopedics Ⅱ, Internal Medicine Ⅰ and Internal Medicine Ⅲin Cangnan Second People's Hospital receiving health education were divided into two groups; both groups received routine training, while in the observation group, on the basis of routine training, the hand hygiene pollution investigation training mode was added, In the combined training method, when the wrong hand hygiene mode occurred, the hand specimens were collected and the correct hand washing method was instructed on the spot, and then the following hand specimens were taken to investigate the contaminated condition. One month before the training, the real situations of hand hygiene compliance in the two groups were investigated. In the 1st month after the beginning of training, both groups were cultivated by conventional propaganda and training methods. From the second month to the sixth month, the observation group was additionally trained to investigate hand hygiene pollution situation on the basis of conventional propaganda and training methods. After 6 months, the compliance rates of hand hygiene in the two groups were observed. Results Before training, there was no significant differences in the compliance rate of hand hygiene in the observation group and the routine trainning group [37.14% (52/140) vs. 36.36% (48/132), P > 0.05]. The compliance rates of hand hygiene after 1, 2, 3 months of training in the observation group were 56.61% (77/136), 60.61% (80/132) and 61.20% (82/134), respectively, and those in the routine trainning group were 56.25% (72/128), 59.26% (80/135) and 58.70% (81/138), which were all significantly higher than those before the training (all P < 0.05), but there were no statistical significant differences between the two groups (all P > 0.05). From the 4th month to the 6th month after training, the compliance rates of hand hygiene in the observation group were significantly higher than those in the observation group [61.97% (88/142) vs. 49.23% (64/130), 62.50% (80/128) vs. 47.73% (63/132), 62.31% (81/130) vs. 46.03% (58/126)], the differences between the two groups being statistically significant (all P < 0.05). Conclusion The routine training combined with hand contamination investigation survey training for consecutive 3 months can nicely promote the improvement of hand hygiene compliance of medical and nursing staffs.

Objective To compare the effect of primary suture and T tube drainage of laparoscopic common bile duct exploration(LCBDE) in the treatment of patients with choledocholithiasis.Methods A total of 57 patients with calculus of extrahepatic bile duct were selected and they were randomly divided into observation group(n =25,treated by LCBDE) and control group(n =32,treated by T tube drainage).The operation time,postoperative recovery time of gastrointestinal tract,abdominal drainage time,postoperative hospital stay,hospitalization expenses and postoperative complications were recorded and compared between the two groups.Results All the patients were successfully completed surgery,and no patients had open surgery.The recovery time and postoperative hospital stay of the observation group were (1.7 ± 0.5) d,(5.3 ± 1.6) d,respectively,which were significantly shorter than (2.1 ± 0.3) d,(9.2 ± 1.9) d of the control group (t =11.73,6.27,all P ＜ 0.05),and the cost of hospitalization of the observation group was (6873 ± 2 541)RMB,which was significantly higher than (6 079 ± 2 492)RMB of the control group (t =5.93,P ＜ 0.05).The operation time and drainage time of the observation group were (86.3 ± 19.6) min,(89.7 ± 21.6) h,respectively,which were shorter than (97.1 ± 21.1) min,(98.2 ± 22.7) h of the control group,but the differences were not statistically significant(all P ＞ 0.05).There were 4 cases of postoperative bile leakage and 1 case of common bile duct residual stones in the observation group,and the incidence rate of complications was 20.00％.There were 2 cases of bile leakage,3 cases of common bile duct residual stones and 2 cases of biliary peritonitis in the control group,and the incidence rate of complications was 21.87％.There was no statistically significant difference in the incidence rate of complications between the two groups (x2 =0.15,P ＞0.05).All the patients were followed up for 1 to 2 years,the recurrence rate of stones in the observation group was 12.00％,which was similar to 15.60％ in the control group (x2 =0.36,P ＞ 0.05).Conclusion Primary suture of laparoscopic common bile duct exploration has the advantages such as less trauma,less pain,faster recovery and shorter hospital stay in the treatment of patients with choledocholithiasis,which is worthy of clinical application and popularization.