1.Evaluation of ocular refraction in silicone oil temponade eyes by the IOL-Master system
Jun, SHAO ; Bijun, ZHU ; Ying, FAN
Chinese Ophthalmic Research 2010;28(3):271-274
Background The combination procedure of the removal of silicon oil and cataract extraction+intraocular lens (IOL) implantation is the main method of treating cataract in silicone oil tamponade eye.However,the conventional measuring method of the IOL power is limiting in eyes with silicone oil tamponade.The IOL-Master system is confirmed to be an ideal non-contact optical measuring instrument in vivo.Objective The aim of this study is to measure the preoperative IOL power,evaluate the accuracy of biometry with the IOL-Master system and compare the relationship between different factors and refractive error.Methods IOL power was measured in 29 silicone oil tamponade eyes of 29 patients prior to the silicone oil removal combining IOL implantation with the IOL-Master system according to different factors such as placement time,axis oculi,complications and so on.The reasons of visual acuity recovery and measurement error were discussed.Results Postoperative visual acuity was improved in all of the patients.The average predictive refractive error was 0.329±0.846 (-1.5--2D).Classified factors for the cause of disease for axis oculi included rhegmatogenous retinal detachment (RRD)(t=0.478,P=0.637),macular hole(t=0.135,P=0.895),myopia(t=0.435,P=0.667)and oil-placed time (good for less than 1 year).These factors showed an indirect relationship with errors.Conclusion Silicone oil removal combined with IOL implantation can improve visual acuity.The IOL-Master biometry is accurate,safe and convenient in measuring the IOL power in silicon oil temponade eye,but future research should be performed to decrease the measuring error.
2.Treatment of aminoguanidine in retina of diabetic of rats with selective inhibits induced nitric oxide synthase
Dawei LUO ; Haidong ZOU ; Kun LIU ; Zhi ZHENG ; Xiaodong SUN ; Xun XU ; Bijun ZHU
Chongqing Medicine 2014;(19):2440-2442
Objective To investigate the treatment and mechanism of aminoguanidine in retina of diabetic of rats .Methods To-tal 60 rats were divided into control group(n=20) ,diabetic group(n=20) and aminoguanidine treatment group(n=20)which would be treated by aminoguanidine for 14 days .Then the eye tissue of rats were took after 14 days administration for pathological obser-vation(HE staining) ,and the induced nitric oxide synthase(iNOS) ,endothelial nitric oxide synthase(eNOS) ,nerve type of nitric ox-ide synthase(nNOS) level and the expression of differences content and expression were investigated by ELISA ,Western blot and PT-PCR .Results HE staining showed that retinal tissue defects decreased and neuronal cells of rats in aminoguanidine treatment group were increased and significant (P<0 .05) compared rats in diabetic group .The iNOS content and expression of rats in amin-oguanidine treatment group were lower than diabetic group by ELISA ,Western blot and PT-PCR ,it was significantly difference (P<0 .05) and without significant difference between the normal group and diabetic group (P> 0 .05) .Compared with diabetes group ,iNOS ,eNOS ,nNOS protein expression in the rat retina in aminoguanidine treatment group were reduced (P< 0 .05) ,and without significant difference between the normal group and aminoguanidine treatment group (P>0 .05) .The iNOS mRNA expres-sion was lower than that of eNOS mRNA and nNOS mRNA in aminoguanidine treatment group .Conclusion Aminoguanidine can improve retinal tissue of diabetic rats with lesions ,the pathways may be selectively inhibit inducible nitric oxide synthase activity of iNOS .
3.Gankyrin involved in nimesulide induced apoptosis of human gastric cancer cells
Yanjie ZHANG ; Bijun WANG ; Peng CHENG ; Yujie BAO ; Liming ZHU ; Wenyan YANG ; Qiang DAI ; Fohu JIANG
Chinese Journal of Digestion 2010;30(1):33-36
Objective To elucidate the expression of gankyrin in human gastric cancer cells and it's role in nimesulide induced apoptosis. Methods Four human gastric cancer cell lines including MKN28 (well differentiated), AGS (poorly differentiated), MKN45 (poorly differentiated), and SGC7901(moderately differentiated) were cultured and treated with nimesulide. Nimesulide induced growth inhibition and apoptosis of the cells were detected by methyl thiazolyl tetrazolium assay, and confirmed by flow cytometry. The expressions of gankyrin gene and protein were further assessed by real-time PCR and Western blotting. Results Gankyrin mRNA and protein were detected in all four human gastric cancer cell lines. The proliferations of AGS and SGC7901 cell lines were significantly suppressed by nimesulide in a time-dose dependent manner. When treated with 400 μmol/L of nimesulide for 48 hours, the significant apoptosis was found in AGS cells (23.30%±2.50%) and SGC7901 cells (16.80%±1.55% ) in comparison with controls (0.57%±0.19% and 0.88%± 0.17%, respectively, all P values <0.01). Apoptosis of AGS cells induced by nimesulide was accompanied by a considerably decreased gankyrin expression that was more significant at 24 hours (0.0035±0.0014) and 36 hours (0.0980±0.0160) in comparison with controls (0.4690±0.1190, all P values<0.01). Conclusion Gankyrin expresses in human gastric cancer cell lines and may be involved in nimesulide induced apoptosis of AGS cells.
4.Application of case-based learning in ophthalmology course and its assessment
Haiyun LIU ; Bijun ZHU ; Kun LIU ; Ying FAN ; Xiaodong SUN ; Xun XU
Chinese Journal of Medical Education Research 2013;(4):325-327
Objective To assess the effect of case-based learning (CBL) in ophthalmology teaching.Methods Lecture based learning combined with CBL was applied in ophthalmology teaching for 19 undergraduates majored in clinical medicine of 2007 grade from Shanghai Jiao Tong University School of Medicine.Questionnaire survey was conducted for students after the course ended to understand their evaluation on CBL.Proportion of selected data was analyzed comparatively.Results 100% (19/19) students believed that CBL was essential in ophthalmology teaching and rational at the end of course.84.2% (16/19) students accounted that CBL could only be assistant teaching method.94.7% (18/19) and 84.2% (16/19) students considered that CBL was beneficial for cognition of eye diseases completely and clinical thinking formation.4-6 class hours for CBL were approved by 73.7% (14/19) students.Conclusions CBL is essential in ophthalmology teaching and is accepted and supported by students,therefore it should be the main assistant teaching method.
5.Deletion of the LMP-1 gene integrated in the nasopharyngeal carcinoma cell line SUNE-1
Peng LIU ; Zhenyu ZHU ; Qingxiu ZHANG ; Bijun HUANG ; Manzhi LI ; Danyun HUANG ; Jianqua MA
Chinese Journal of Pathophysiology 2000;0(08):-
AIM: To study the EBV LMP-1 gene integrated in the chromosome of poorly differentiated nasopharyngeal carcinoma cell line SUNE-1. METHODS: The LMP-1 gene of SUNE-1 was detected with PCR; Deletion of LMP-1 was examined by restriction endonuclease analysis and PCR. The deletion was precisely localized by DNA sequencing. RESULTS: The LMP-1 gene integrated in the chromosome of SUNE-1 could be deleted or non-deleted. The two introns of LMP-1 gene were shown being lost in SUNE-1 cell line. CONCLUSION: Deletion of intron 1 and intron 2 happen in some of the LMP-1 gene integrated in the chromosome of SUNE-1.
6.Analysis and thinking on the post marketing quality of the disposable sterilized syringe.
Jingli LI ; Wanjuan YANG ; Qing HAO ; Yuan JI ; Bijun ZHU
Chinese Journal of Medical Instrumentation 2012;36(6):441-443
By scattered-sampling testing the disposable sterilized syringe according to legal inspection and explorative research methods of vitro cytotoxicity and easy oxide etc. testing, this article comprehensively evaluated and analyzed the product quality and found the potential risk. The results will help to improve the work process and product quality.
Disposable Equipment
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Product Surveillance, Postmarketing
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Quality Control
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Syringes
7. A retrospective study of docetaxel combined with ADT therapy in the treatment of metastatic hormone-sensitive prostate cancer
Bijun LIAN ; Jing LI ; Huan CHEN ; Feng ZHU ; Min QU ; Yan WANG ; Zepeng JIA ; Xu GAO
Chinese Journal of Urology 2020;41(1):26-31
Objective:
To investigte the efficacy of docetaxel combined with androgen deprivation therapy for the treatment of metastatic hormone-sensitive prostate cancer based on Chinese population.
Methods:
A total of 497 patients were enrolled from January 2004 to July 2018 in the Changhai Hospital. 459 patients received androgen deprivation therapy alone and 38 patients received androgen deprivation therapy combined with docetaxel. The mean age was (72.1±8.7)years. The median PSA level was 100.0 ng/ml, ranging 42.3-999.0 ng/ml. Patients of clinical T2, T3, T4 stage were 213(42.9%), 160(32.2%), 124(24.9%), respectively. Patients of clinical N0, N1, Nx stage were 319(64.2%), 144(29.0%), 34(6.8%), respectively. Patients of clinical M0, M1a, M1b, M1c, Mx stage were 100(20.1%), 51(10.3%), 332(66.8%), 9(1.8%), 5(1.0%), respectively. Gleason scores of biopsy showed that 146(29.4%) patients was ≤7, 103(20.7%) was 8 and 248(49.9%)was ≥9. Propensity score matching was used to match the baseline between groups. Caliper value was set at 0.02. SPSS 22 software was used to achieve a 1∶1 match between the two groups. There were no statistical difference in the age(
8.Analysis on the screening and follow-up of cytomegalovirus infection in infants in Lishui
Chenfu LAN ; Sipeng LI ; Xiaohong XU ; Shaonan SHEN ; Yanhua ZHONG ; Guanjin CHEN ; Junsheng LI ; Xiaohong WANG ; Ruying LAN ; Aolin ZHANG ; Bijun ZHU ; Yahong ZHOU
Chinese Journal of Postgraduates of Medicine 2020;43(8):678-685
Objective:To investigate the current situation of cytomegalovirus (CMV) infection in infants in Lishui, and summarize the related factors of CMV infection, evaluate its influence on the growth and development of infants, and provide evidence for the prevention and control of CMV infection.Methods:In this study, 2 254 cases of infants admitted in pediatric ward in Lishui Maternal and Child Health Hospital, Qingtian County People′s Hospital, Suichang County People′s Hospital, Qingyuan County People′s Hospital from January 1, 2015 to December 31, 2017 with integral clinical data were selected. All the babies were followed up from the time when they were born to 1 year old. The serum CMV antibody and the urine CMV-DNA were screened, the general situation and clinical features of CMV infection were summarized, and the relevant factors of infants CMV infection were analyzed and screened by the single factor and multiple factors analysis. They were followed up to 1 year old to clarify the influence of CMV infection on the growth and development of infants.Results:From 2015 to 2017, the total positive infection rate of CMV-IgM in infants under 1 year old in Lishui was 10.43%(235/2 254), and CMV-IgM positive infection decreased year by year. The positive rate of CMV-IgG did not change significantly with time. The positive rate of CMV-IgM was the highest at 1—3 months, and up to 15.29% (61/399). The positive rate of CMV-IgM decreased with the age of the babies. The positive rate of CMV-IgG increased with the age of the babies. The positive rate of CMV-IgM in infants showed no significant difference in gender ( P>0.05). The positive rate of CMV-IgM was higher in men than that in women [65.43% (810/1 238) vs. 55.51% (564/1 016)], and there was significant difference ( P<0.05). The gestational age of the infected group was lower than that of the non-infected group [(37.41 ± 1.63) weeks vs. (38.97 ± 0.97) weeks], and the breast-feeding rate of the infected group was higher than that of the non-infected group [57.87%(136/235) vs. 40.00%(40/100)], and there were significant differences ( P<0.05). Thrombocytopenia, the increase of transaminase, necrotizing enterocolitis of newborn, and hepatosplenomegaly of infected group is higher that of the non-infected group [18.72%(44/235) vs. 1.00% (1/100), 29.36% (69/235) vs. 13.00% (13/100), 26.81% (63/235) vs. 10.00% (10/100), 9.79% (23/235) vs. 0], and there were significant differences ( P<0.05). Gestational age and breast-feeding were possible risk factors for CMV infection in infants under 1 year old ( P<0.05). There was no significant difference in height, weight, head circumference and intelligence score between the infected group and the non-infected group at the age of 1 year ( P>0.05). The total abnormal rate of hearing development and the abnormal detection rate of B-ultrasound in the infected group were higher than those in the non-infected group [13.62%(64/470) vs. 1.00%(2/200), 6.38%(15/235) vs. 0], and there were significant differences ( P<0.05). Conclusions:The CMV active infection rate of infants under 1 year old in Lishui is relatively high and decreases year by year. It decreases with the prolongation of birth time, and there is no gender difference. Gestational age and breast-feeding are the risk factors for active CMV infection in infants. CMV infection affects the hearing development and the brain development of infants under 1 year old, which is the main cause of hepatitis. It is necessary to pay attention to the prevention of CMV infection, strengthen maternal perinatal health care, and strengthen the screening of CMV infection in high-risk groups.