Objective: To study the effectc of different growing areas and seasonal variation on the components and contents of Ginkgo Biloba terpene lactones. Methods: The contents and components of different Ginkgo Biloba terpene lactones were determined by RP-HPLC. Results: The contents of Ginkgo Biloba terpene lactones among the samples of Ginkgo Biloba Leaves of different growing areas were obviously different, the terpene lactones content of Ginkgo Biloba Leaves growing in Zaoyang county, Hubei Province, was higher. Based on the monthly variation of the contents of different Ginkgo Biloba terpene lactones, the suitable seasons for the harvest of Ginkgo Biloba Leaves were from Sep. to Oct. Conclusion: The growing areas and seasonal variation had great influence over the contents and components of Ginkgo Biloba terpene lactones.