2.Establishment and identification of L783V/3T3 cell line infected with leukemia viruses
Chinese Journal of Pathophysiology 1989;0(06):-
L783 murine lymphocytic leukemia was established in 1978 by Dept. of Pathophysiology of Shanghai Medical University. L783V/3T3 cell line was established by infecting NIH-3T3 cells with the cell-free extracts of blood, spleen respectively of L783 mice. The plaque assay of L783V/3T3 cells was positive, typical A-type and C-type virus particles could be observed under electronmicroscope. Lymphocytic leukemia could be induced by inoculation of cell-free extracts from L783V/3T3 cells into Kunming strain newborn mice.
3.The exploratory development of mental intervention combined with simple brachial plexus anesthesia in children'upper extremity operation
Qinquan JI ; Biao JIANG ; Hongwei LIN
Chinese Journal of Primary Medicine and Pharmacy 2011;18(5):610-611
Objective To evaluate the effect of mental intervention combined with simple brachial plexus anesthesia in children' upper extremity operation. Method 80 cases of8 ~ 12 years old children were selected after strict evaluation and screening,of who needed upper extremity operation(fractures internal fixation not included) ,and the surgery time less than 120 minutes. All the operations were completed under the simple brachial plexus anesthesia, and combined with mental intervention preoperatively and intraoperatively. The changes of HR and BP intraoperatively the psyche condition after the operation completed were observed and followed up the mental change postoperatively. Result All the operation were completed. The HR and BP were stable intraoperation (P > 0. 05), the psyche was quiet after the operation completed,and no mental disorder was happened postoperative. Conclusion The mental intervention combined with simple brachial plexus anesthesia was safe in 8 ~ 12 years old children' upper extremity operation, and it deserved to spread and apply.
4.Clinical analysis of QT interval dispersion in predicting susceptibility to ventricular tachycardia in patients with coronary heart disease
Yong JI ; Biao FAN ; Weiqun WANG
Chinese Journal of Practical Internal Medicine 2002;0(08):-
0 05) for inducible VT; Utility of QTD≥70ms combined with positive VLP had a more satisfied outcome than using either of these abnormalities in predicting inducible VT. Conclusion QTD is an easily measurable electrocardiographic index for predicting coronary heart disease.
5.Study on the release of copper ion and corrosion for intrauterine contraceptive device with two kinds of Cu-containing stent.
Fengping ZOU ; Shuze HUANG ; Rong JI ; Biao DING ; Tianping YAO
Chinese Journal of Medical Instrumentation 2014;38(3):222-224
Two kinds of Cu-containing intrauterine contraceptive device (Cu-IUD) has been evaluated through the study on the release of copper ion and corrosion of copper surface in artificially formulated fluid. The result showed that the copper ions were released profusely from the stainless steel stents Cu-IUD in the first week of soak (25-12 microg/d), and then it became slowly and steady. The release of the copper ions from the titanium nickel shape memory alloy stents Cu-IUD were always slowly and steady in two months (0.5-5 microg/d). It obviously shows that the corrosion on copper surface of stainless steel stents Cu-IUD were more serious than titanium nickel shape memory alloy stents Cu-IUD by using 200 times microscope testing before and after soaking in formulated fluid.
Copper
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chemistry
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Corrosion
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Intrauterine Devices
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Ions
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chemistry
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Stents
7.Application of degree of portal systemic shunting in assessing upper gastro-intestinal bleeding in patients with schistosomiasis cirrhosis
Shuai JU ; Ying LI ; Changxue JI ; Biao ZHANG
Chinese Journal of Schistosomiasis Control 2017;29(3):286-289
Objective To discuss the application of the degree of portal systemic shunting in assessing the upper gastrointes-tinal bleeding in patients with hepatic schistosomiasis. Methods Thirty - three patients with upper gastrointestinal bleeding caused by hepatic schistosomiasis(a bleeding group)and 29 schistosomiasis cirrhosis patients without bleeding(a non-bleeding group)were enrolled as investigation subjects in Jinshan Hospital. The subjects were scanned by the 128 abdominal slice spiral CT. The portal systemic shunting vessels were reconstructed by using thin slab maximum intensity projection(TSMIP)and multi-planar reconstruction(MPR). The degrees of the shunting vessels of the subjects were evaluated and compared,and the relation-ship between upper gastrointestinal bleeding and the degree of the shunting was analyzed. Results In the bleeding group,the occurrence rates of the shunting vessels were found as follows:86.4% in left gastric varices,68.2% in short gastric varices, 50.0% in esophageal varices,50.0% in para-esophageal varices,37.9% in gastric varices,69.7% in gastric-renal varices, 51.5% in spleen-renal varices,25.8% in abdominal wall varices,15.2% in omentum varices,63.6% in para-splenic varices, 34.8% in umbilical varices,40.9% in retroperitoneal-paravertebral varices,and 36.4% in mesenteric varices. In the bleeding group,the occurrence rates and the degree of shunt were significantly higher than those in the non-bleeding group in esophageal varices,esophageal vein,left gastric vein and gastric varices(all P < 0.05). Conclusions CT portal vein reconstruction can accurately display the location,degree and walking of all kinds of shunting vessels. Esophageal varices,esophageal vein,left gastric vein and gastric varices can accurately predict the risk of upper gastrointestinal bleeding in patients with hepatic schisto-somiasis. The patents with higher degree of the shunting vessels have a higher risk of gastrointestinal bleeding.
8.Relative and Absolute Quantification-Expression Analysis of CsSAMDC Gene As a Case
Ji-Yu ZHANG ; Yan-Rong WANG ; Zhi-Biao NAN ;
China Biotechnology 2006;0(08):-
This experiment was based on a full-length SAMDC sequence from the drought stress induced cDNA library of Cleistogenes songorica.Two methods,absolute quantification and relative quantification,were used to analyze the gene expression under different tissue and drought stress treatment by real-time quantitaive PCR experiments and were compared each other,while CsSAMDC gene was used as a case.Leaves and roots tissue were sampled from the plants at 0,4,6,8,and 10 d of drought stress and 1 and 4 d after rewatering.The 2-CT method was used to analyze the relative changes in gene expression from quantitative real-time PCR experiments.A standard curve was generated by the purified plasmid DNA.The primer concentration in the realtime PCR was optimized.All absolute quatification data were normalized by the normalization factor from the most stable house-keeping genes in C.songorica.Relative quantification results using 2-CT method showed that CsSAMDC transcripts increased 5.93?0.71 fold in roots,down regulated 0.62?0.13 fold in leaves,under drought stress.Absolute quantification results showed that CsSAMDC transcripts up-regulated in roots significantly under drought stress as 1ong as 8 to 1 0 days after drought stress,while down-regulated in both roots and leaves after drought stress within 6 days and 1 to 4 days after rewatering,compared with control.CsSAMDC transcipt showed similar tendency of gene expression compared by using relative and absolute quantification methods.
9.ANALYSIS OF PYROLYSIS GAS CHROMATOGRAPHY OF SALMONELLA TYPHI
Biao JI ; Peishang REN ; Yin TANG ; Hongen XIONG ; Li TAN ;
Microbiology 1992;0(06):-
The chromatograms of pyrolysis gas chromatography (PGC) of fifteen strains of Salmonella typhi were reported to see if there is any possible relation of the graphs to serological typing and drug-resistance spectra of the bact. In the fifteen strains there were fourteen clinical strains and one reference strain (Hg01). The results indicated that the clinical strains might be divided into group A and B (7 strains each) by PGC. The graphs drawn from group A organisms were entirely the same in shape while those of group B showed only basic similarity. According to the diversity of graphs of group B organisms, the group B organisms, may be subdivided into group B1 (4 strains), B2 (2 strains) and B3 (1 strain). The graphs within each subgroup were completely the same and those of subgroup B1 exhibited close similarity to that of the reference strain. Albeit no regular connection of chromatograms with serological types and drug-resistance could be seen, the graphs yet provided useful reference for founding a bank of chromatograms, compaed with which salmonella typhi graph could then be identified.
10.Analysis on the reasons for diffuse 18F-FDG uptake by bone marrow using whole-body PET/CT
Ji-lin, YIN ; Xin-lu, WANG ; Jin-he, ZHANG ; Yu, LIU ; Wei-biao, ZHANG ; Xi, OUYANG
Chinese Journal of Nuclear Medicine 2011;31(3):151-154
Objective To analyze the causes for diffuse bone marrow uptake of 18F-FDG on PET/CT scans. Methods Sixty-six patients with diffuse bone marrow uptake on whole-body FDG-PET/CT imaging were enrolled for this study. Seventy-nine healthy subjects ( with no history of tumor or recent fever) were selected as normal control. The SUVmax and SUVmean were measured in bone marrow and mediastinum in both groups. The maximum (bone marrow SUVmax/ mediastinum SUVmax) and mean value ratios (bone marrow SUVmean/ mediastinum SUVmean) were calculated. Statistical analysis was performed by one-factor variance analysis. Results With diffuse bone marrow uptake pattern of 18F-FDG, 27 were caused by injection of hematopoietic growth factor, 21 by hematopathy and 18 due to fever. SUVmeanof those three causes were 3.076±1.955, 3.633±2.405 and 2.546±0.791 respectively, each was significantly different from that of the control group (1.026±0.190; F =34.465, P<0.001). Conclusion Diffuse bone marrow uptake on FDG-PET/CT are caused by both benign and malignant reasons.