Cisplatin is a first-line anticancer drug widely used in clinic. However, its resistance reduces its efficacy. With a non-specific cell cycle, cisplatin's main targets are nucleophilic protein, DNA and RNA in cells. Among cisplatin's multi-factorial resistance mechanisms, abnormal expression of transport protein, intracellular detoxification enhancement, DNA repair capacity increase and apoptosis blocking are the main mechanisms. Because traditional Chinese medicines (TCMs) have unique advantages in cancer treatment, their combination with cisplatin can improve the efficacy. In this paper, the authors summarized the advance in studies on cisplatin's resistance and the combination of TCMs and cisplatin in recent years.
Antineoplastic Agents ; administration & dosage ; pharmacology ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Apoptosis ; drug effects ; Cell Cycle ; drug effects ; Cisplatin ; administration & dosage ; pharmacology ; Drug Resistance, Neoplasm ; drug effects ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Medicine, Chinese Traditional ; methods ; trends ; Neoplasms ; drug therapy ; pathology ; therapy

0.05) in tail flick test.MPE% in group MK was always higher than group M and descended more slowly than group M,especially from the d4 to d8(P0.05). Conclusion Ketamine could block the development of morphine tolerance partly due to its inhibition effect on pCREB protein.

Animals ; Gene Expression Regulation ; drug effects ; Gene Targeting ; methods ; Humans ; Oligonucleotides, Antisense ; genetics ; pharmacology ; RNA Interference ; RNA, Small Interfering ; genetics ; Transfection

Adolescent ; Adult ; Cholecystitis ; complications ; drug therapy ; surgery ; Cholecystolithiasis ; complications ; drug therapy ; surgery ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Pancreatitis ; drug therapy ; etiology ; surgery ; Phytotherapy ; Postoperative Period ; Sphincterotomy, Endoscopic

Objective:To explore the correlation among morning blood pressure surge (MBPS) ,ambulatory arterial stiffness index (AASI) and carotid artery intima‐media thickness (IMT) .Methods :A total of 212 hypertension pa‐tients received 24h ambulatory blood pressure monitoring and carotid artery ultrasonography to measure carotid IMT .According to IMT thickened or not ,they were divided into normal IMT group (n=84) and thickened IMT group (n = 128) ,then MBPS and AASI were calculated .According to MBPS existed or not ,patients were divided into MBPS group (n = 120) and no MBPS group (n = 92) ,then the correlation among MBPS ,AASI and carotid IMT were observed .Results : Compared with normal IMT group ,there were significant rise in AASI [ (0.42 ± 0.13) vs .(0.68 ± 0.14)] and MBPS value [ (25.94 ± 4.57) mmHg vs .(36.57 ± 8.41) mmHg] in thickened IMT group , P < 0.05 both ;compared with no MBPS group ,there were significant rise in AASI [ (0.43 ± 0.13) vs .(0.71 ± 0.14)] and IMT [(1.01 ± 0.20) mm vs .(1.25 ± 0.17) mm] in MBPS group ,P < 0.05 both ;Linear correlation analysis indicated that MBPS and AASI were positively correlated with carotid IMT ( r = 1.22 ,0.51 , P < 0.05 both) .Conclusion : Morning blood pressure surge and ambulatory arterial stiffness index are independent risk factors for carotid artery intima‐media thickness ,and they possess predictive value .

Medical implant communication service (MICS) is a standardized short-range wireless communication with ultra low power consumption applied for medical implant devices,which has been widely used all over the world.It is necessary to establish a unified international MICS standard.This paper details ITU-R SA.1346 standard from four aspects of technical parameters,compatibility,anti-interference methods and reliability of data transmission,and introduces the applications of MICS standard in Europe and America.Furthermore,comparison and analysis of the technical criteria in the above areas is conducted.

Objective To investigate the dynamic changes of Trk(tropomyosin receptor kinase) and Akt(protein kinase B), and to explore the neuroprotective role and its mechanism of β-NGF (β-nerve growth factor) after focal cerebral ischemia. Methods A focal cerebral ischemia rat model was made and β-NGF was injected intra abdominally. The phosphorylation of Trk and Akt were tested by immunocytochemistry and Western blot assay. Results Trk receptor was increased obviously in the penumbra area after 8 h of infarction. The level of phosphorylated Trk(p-Trk) was increased after 2 h of infarction, while the level of phosphorylated Akt(p-Akt) had a decrease and then recovered gradually. The level of p Akt in cerebral ischemia group was decreased by 76.5% compared with that in control group after 8 h of infarction(P<0. 01). After the injection of β-NGF, the level of p-Trk was increased by 74.4% after 12 h of infarction(P<0.01), while the level of p-Akt was recovered significantly after 8 h of infarction, and had no statistical difference compared with the control group after 24 h of infarction (P>0.05). Conclusions Ischemia induces the activation and increased expression of Trk receptor, andβ-NGF may play a protective role in cerebral ischemia by increasing the phosphorylation of Trk and regulating the phosphorylation of Akt.

Objective To evaluate the criteria for diagnosis of atypical squamous cells(ASC)and its two subtypes: undetermined significance ASC (ASC US) and atypical squamous cells cannot exclude high grade intraepithelial lesion (HSIL, ASC H) defined in 2001 the Bethesda system(TBS) Method One thousand one hundred and eighty six pap smears and liquid based thin layer preparations with diagnosis of atypical squamous cells of undetermined signification (ASCUS) according to TBS 1991 criteria, were re classified by a cytopathologist using TBS 2001 criteria Results The cytologic diagnosis according to TBS 2001 criteria was: negative in 285 cases(24 0 %), ASC US in 627 cases (52 9 %), ASC H in 254 cases(21 4 %), low grade intraepithelial lesion (LISL) in 16 cases(1 3 %), and HISL in 4 cases (0 3 %), respectively Out of 1186 cases, 168 received histological examination Cervical intra epithelial neoplasia (CIN) II III were diagnosed in 18 9 % of patients with ASC H, 5 2% of patients with ASC US, and 14 3% of patients with ASCUS defined by using TBS 1991 criteria, respectively ( P

Objective To research the value of dynamic CT enhancement in diagnosing lung cancer.Methods 35 patients with benign disease and 25 patients with lung cancer underwent dynamic CT scan,the results of CT were analysed in comparison with that of pathological specimens,the relationship between the dynamic CT enhancement phenomena and histological results.Results CT enhanced peak,the enhanced ratio of focus and aorta,the form of enhancement and time-dense curve were different between two groups.CT enhanced peak was related to the histological types and clinical stages of pulmonary cacinoma,and metastasis of lymph nodes.Conclusion Dynamic CT enhanced scan can reflect the characteristic of blood in lung cancer.The invasion and metastasis in lung carcinoma can be predicted according to the CT enhanced peak.

Objective To develop a method for determining cyanogen chloride (CNCl) in drinking water. Methods Pyridine-barbituric acid spectrophotometry was employed and some parameters including the pH value of the phosphate buffer, the time of color reaction, temperature, the concentration of chloramine T, the proportion of the phosphate buffer, the proportion of color agent and the maximum absorption wavelength were optimized. The linear range, limit of detection, precision and recovery rate were studied. Results Trace quantity of CNCl obeyed the Law of Beer. In the range of 0-0.1 ?g/ml CN-, the correlation coefficient was 0.999 4, the regression equation was y=0.194x-0.033, the limit of detection was 0.003 ?g/L, the lower limit of detection was 0.01 mg/L, the relative standard deviations were 1.5%-6.2% and the recovery rates were 92.9%-107.1%. Conclusion The method has the characters of accurate, sensitive, simple and rapid, it can be used in monitoring of drinking water quality.